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Journal : Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal

Hubungan Kekerabatan Fenetik Jamur Shiitake (Lentinula edodes (Berk.) Pegler) berdasarkan Karakter Morfologi Ekowati, Nuraeni; Kasiamdari, Rina Sri; Pusposendjojo, Nursamsi; Soegihardjo, C. J.
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.267

Abstract

Phenetic relationship of shiitake mushroom (Lentinula edodes (Berk.) Pegler) cultivated in Indonesia especially in Java is still undisclosed. In order to increase adaptability and high production most of cultivators in Indonesia make inter-isolates breeding. Consequently  L. edodes isolates grown in Indonesia may have morphological and genetic diversity. Diversity among the isolates of L.edodes can be determined by morphological and molecular assessments. Isolates originating from different locations can show specific morphological characters, and requires more in-depth assessment of the macroscopic and microscopic morphological characters. Four  L.edodes isolates from Malang, Cianjur, Lembang and Yogyakarta were understudy to determine their morphological characters and phenetic relationship. Degree of similarity between the isolates was carried out through numerical taxonomy approach and the data obtained were analyzed using the Numerical taxonomy program and multivariate analysis system, version 2.1. The results showed that there was diversity of morphology of the four isolates and based on the results of data analysis obtained, the degree of similarity between the isolates ranged between 83-95%. Isolates of L. edodes from Malang to Cianjur showed the closest phenetic relationship (95%) and isolates from Malang to Yogyakarta showed the most distant phenetic relationship (83%). Based on the morphological characters obtained from this research revealed the diversity and phenetic relationship among the four isolates of L. edodes.
Potensi Daun Trembilungan (Begonia hirtella Link) sebagai Antibakteri dan Antifungi Ngazizah, Febri Nur; Ekowati, Nuraeni; Septiana, Aisyah Tri
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 33, No 3 (2016)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2016.33.3.309

Abstract

Begonia hirtella leaf are commonly used by people in mountainous areas to treat itchy due to insect bites and skin infection. This study aims to (1) determine proper solvent extract of B. hirtella leaf on antibacterial activity of S. aureus and E. coli and on antifungal activity of C. albicans (2) determine the lowest concentration of leaf extract of B. hirtella that indicate the formation of inhibition zone (3) know the content of bioactive compounds contained in each leaf extract of B. hirtella. The method used in this study is experimental with completely randomized design (CRD). The treatments were type of solvents (n-hexane, ethyl acetate, ethanol and water) and a serie of the ethyl acetate extract concentration of 500 ppm, 450 ppm, 400 ppm, 350 ppm, 300 ppm and 250 ppm against S. aureus, E. coli and C. albicans. Data were analyzed using analysis of variance (Anova) and the significant differences between the treatments were analyzed by Duncan test at 95% confidence level. The result showed that each solvent extracts affect microbes growth with a highly significant difference p <0.05. Solvent extracts that have the best inhibitory zone is ethyl acetate against S. aureus with an average diameter of inhibitory zone 13.75 ± 1.26 mm. Increasing concentrations of ethyl acetate extract of 250 ppm to 500 ppm increase inhibition zone against microbes. The lowest concentration that show inhibition zone was 300 ppm. The formation inhibition of zone on microbes growth happened due to their compounds in the extract. Extract of n-hexane contains stigmasterol, ethyl acetate contains neophytadiene, while the ethanol extract contains ethyl palmitate
Aktivitas Antioksidan Ekstrak Tubuh Buah Jamur Paha Ayam (Coprinus comatus) dengan Pelarut Berbeda Susanto, Agus; Nuniek, Nuniek Ina; Ekowati, Nuraeni
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 35, No 2 (2018)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2018.35.2.566

Abstract

Coprinus comatus or with local name is  drum stick chiken mushroom that includes edible and medicinal mushroom. This mushroom contains bioactive compounds that have several pharmacological effects, like as antibacterial, anticancer, immunomodulator, anti-inflammatory, prevent diabetes and antioxidant. bioactive compounds includes in fruit body C. comatus can be obtained by extraction used solvent. The objective of this study was to determine whether the C. comatus fungus was potential as an antioxidant producer and to know which solvent produced the best antioxidant activity from the extract of the fruit body of C. comatus. The method used in this research is experimental with two treatments ie n-hexane and ethanol 70% solvent. Fruit body of C. comatus is extracted by maceration method, then the viscous extract obtained by phytochemical test and antioxidant activity test using DPPH method (1,1-diphenyl-2-picrihidazil). The results obtained are then analyzed using t-test for independent samples. The results showed that the average yield of ethanol extract 70% higher than n-heksan  were each 61.33% ± 9,21 and 14.8% ± 0,87. The test of total phenol extract ethanol was 70% higher than n-hexane , each with an average of 325,19 ppm ± 50,01 and 110,08 ppm ± 34,67. The result of antioxidant activity test using DPPH method of ethanol extract 70% and n-heksan has IC50 value 2,48 mg/ml and 3,86 mg/ml. Phytochemical test results for flavonoid test showed positive result for both samples extract, terpenoid test showed positive result in both samples , the tannin test showed negative results  in both samples.