Mae Sri Hartati, Mae Sri
Faculty of Medicine Universitas Gadjah Mada Yogyakarta

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EFEK ANTIANGIOGENIK EKSTRAK METANOL AKAR PASAK BUMI (Eurycoma longifolia, Jack) PADA MEMBRAN KORIO ALANTOIS (CAM) EMBRIO AYAM YANG TERINDUKSI bFGF Salamah, Nina; Sugiyanto, Sugiyanto; Hartati, Mae Sri; Hayati, Farida
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.799 KB) | DOI: 10.22146/tradmedj.8062

Abstract

Angiogenesis merupakan peristiwa pertumbuhan pembuluh darah baru, yang memungkinkan sel kanker mendapatkan suplai nutrisi dan oksigen, sehingga dapat terus bertahan hidup. Pasak bumi (Eurycoma longifolia, Jack) merupakan salah satu tanaman asli Indonesia yang memiliki potensi anti kanker. Ekstrak metanol, butanol, kloroform, dan air dari akar pasak bumi terbukti memiliki efek sitotoksik terhadap beberapa sel kanker. Penelitian ini bertujuan untuk mengetahui efek antiangiogenik ekstrak metanol akar pasak bumi pada CAM embrio ayam. Uji penghambatan angiogenesis dilakukan dengan membagi telur berembrio umur 8-9 hari dalam 8 kelompok perlakuan. Kelompok I sebagai kontrol paper disc, kelompok II sebagai kontrol bFGF, kelompok III sebagai kontrol bFGF+ pelarut DMSO 0,8%, kelompok IV, V, VI, VII dan VIII sebagai kelompok uji penghambatan angiogenesis, pada paper disc diberi bFGF 1 ng/μL dan ekstrak metanol akar pasak bumi berturut-turut dengan dosis 20, 40, 60, 80 dan 100 μg/mL. Setelah diinkubasi selama 3 hari (umur embrio12 hari), telur dibuka dan isi telur dikeluarkan, kemudian CAM yang melekat pada cangkang diamati secara makroskopik dan mikroskopik. Ekstrak metanol akar pasak bumi memberikan aktivitas antiangiogenik mulai kadar 40 μg/mL. Peningkatan konsentrasi ekstrak metanol akar pasak bumi meningkatkan aktivitas penghambatan angiogenesis.
EFEK ANTIANGIOGENIK EKSTRAK METANOL AKAR PASAK BUMI (Eurycoma longifolia, Jack) PADA MEMBRAN KORIO ALANTOIS (CAM) EMBRIO AYAM YANG TERINDUKSI bFGF Salamah, Nina; Sugiyanto, Sugiyanto; Hartati, Mae Sri; Hayati, Farida
Majalah Obat Tradisional Vol 15, No 1 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (618.799 KB) | DOI: 10.14499/mot-TradMedJ15iss1pp%p

Abstract

Angiogenesis merupakan peristiwa pertumbuhan pembuluh darah baru, yang memungkinkan sel kanker mendapatkan suplai nutrisi dan oksigen, sehingga dapat terus bertahan hidup. Pasak bumi (Eurycoma longifolia, Jack) merupakan salah satu tanaman asli Indonesia yang memiliki potensi anti kanker. Ekstrak metanol, butanol, kloroform, dan air dari akar pasak bumi terbukti memiliki efek sitotoksik terhadap beberapa sel kanker. Penelitian ini bertujuan untuk mengetahui efek antiangiogenik ekstrak metanol akar pasak bumi pada CAM embrio ayam. Uji penghambatan angiogenesis dilakukan dengan membagi telur berembrio umur 8-9 hari dalam 8 kelompok perlakuan. Kelompok I sebagai kontrol paper disc, kelompok II sebagai kontrol bFGF, kelompok III sebagai kontrol bFGF+ pelarut DMSO 0,8%, kelompok IV, V, VI, VII dan VIII sebagai kelompok uji penghambatan angiogenesis, pada paper disc diberi bFGF 1 ng/μL dan ekstrak metanol akar pasak bumi berturut-turut dengan dosis 20, 40, 60, 80 dan 100 μg/mL. Setelah diinkubasi selama 3 hari (umur embrio12 hari), telur dibuka dan isi telur dikeluarkan, kemudian CAM yang melekat pada cangkang diamati secara makroskopik dan mikroskopik. Ekstrak metanol akar pasak bumi memberikan aktivitas antiangiogenik mulai kadar 40 μg/mL. Peningkatan konsentrasi ekstrak metanol akar pasak bumi meningkatkan aktivitas penghambatan angiogenesis.
Cytotoxicity testing of alkaloid compounds isolated from sponge Petrosia sp: its potency for development of anticancer agent Astuti, Puji; Alam, Gemini; Hartati, Mae Sri; Sari, Dinar; Wahyuono, Subagus
INDONESIAN JOURNAL OF PHARMACY Vol 16 No 1, 2005
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (170.509 KB) | DOI: 10.14499/indonesianjpharm0iss0pp58-62

Abstract

Cancer is still a major problem and common cause of death around the world. Various therapeutic agents have been developed to fight against cancer, but none of these agents give satisfactory results and without debilitating side effects. A number of researches have been conducted to search anticancer compounds with renewed vigour.Sponges, marine invertebrates, are known as rich sources of compounds which pronounced pharmacological activities. The aims of this study are to determine cytotoxic effect of two toxic compounds isolated from chloroform fraction of Petrosia sp sponges collected from Bunaken on myeloma cells.The two toxic compounds were isolated based on bioassay guidedisolation on brine shrimp larvae. Isolation was conducted using column chromatography followed by preparative TLC. Cytotoxic effect of the two compounds was conducted in 96 well plate using RPMI 1640 as medium. The number of viable cells was determined using MTT assay and LC50 (μg/mL) of the compounds was analysed using probit analysis.The results showed that the two compounds were alkaloid and toxic to larva A. salina with LC50 of 7.23 (compound 1) and 5.69 μg/mL (compound 2). These compounds were also toxic to myeloma cells with LC50 values of 16.95 μg/mL (compound 1) and 18.8 μg/mL (compound 2). The longer the incubation time, the compounds were more toxic as showed by the lower LC50 values .Key words: cytotoxic, Petrosia sp, Artemia salina Leach, myeloma
Isolation and identification of eurycomanone from akar pasak bumi (Eurycoma longifolia, Jack) and its antiangiogenic activity ., Sugiyanto; Salamah, Nina; Hartati, Mae Sri; Hayati, Farida; Jumariyatno, Pinus
INDONESIAN JOURNAL OF PHARMACY Vol 20 No 3, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (371.242 KB) | DOI: 10.14499/indonesianjpharm0iss0pp118-126

Abstract

Pasak bumi is one of the Indonesian original plants having anti cancer activity. One compound of akar pasak bumi, i.e eurycomanone was proven to have cytotoxic effect on cancer cell culture by apoptosis induction on HeLa cells. Therefore, this study was aimed to isolate and identify euycomanone from akar pasak bumi and to look at the anti angiogenic activity of the compounds on corio alantois membrane (CAM) of chicken embryo induced by bFGF.The akar pasak bumi was ground and was macerated with methanol. The macerate was fractioned by mean of VLC. Six fractions was obtained. The second fraction exhibited two spots on a TLC system, one spot was cochromatographed with eurycomanone as the reference standart and another spot exhibited blue fluorecence at 254 nm. This second fraction, then, was chromatographed on a preparative scale TLC to separate isolate A. Anti angiogenic test was performed for Isolate A at concentration series of 5, 10, 15, 20, 40 μg/mL respectively. Egg embrio (8-9 days) were divided into 8 groups. The first group was implanted paper disc only, the second group were given bFGF on Tris HCl buffer and the third group were given bFGF (on Tris HCl buffer) + 0,8 % DMSO. The fourth to eighth group were induced by 10 ng bFGF and the test preparation at the respective concentration. After incubation at 39 °C for 3 days, each egg was opened, the content of each was aspired and discarded, the CAM sticked to its shell was examined macroscopically and microscopically for the formation of new blood vessels.Based on spectral data of UV, IR, 1H NMR, 13C NMR and MS of isolate A and comparison with the spectral data of the standart, the isolate A was eurycomanone. Eurycomanone inhibited angiogenesis at concentration of 10 μg/mL.Key words: antiangiogenic, angiogenesis, E.longifolia, eurycomanone,CAM.