Nurhayana Sennang, Nurhayana
Bagian Ilmu Patologi Klinik FK UNHAS–BLU RS DR Wahidin Sudirohusodo, Makassar,

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VANCOMYCIN RESISTANT STAPHYLOCOCCUS AUREUS (VRSA) IN DR. WAHIDIN SUDIROHUSODO HOSPITAL MAKASSAR Ahmad, Fatmawaty; Sennang, Nurhayana; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1385

Abstract

Vancomycin Resistant Staphylococcus aureus (VRSA) refers to bacteria that have complete resistance to Vancomycin. The increasing prevalence of VRSA can be a significant clinical problem with the limited therapeutic options. Therefore, the identification of? VRSA is necessary to improve the management of antibiotic therapy, infection control and to prevent the occurrence of wider resistance. This retrospective descriptive study used secondary data of antibiotic susceptibility test on Staphylococcus aureus identified using VITEK 2 and patient medical records from January 2015 to December 2016. The data is processed using SPSS. Of 387 Staphylococcus aureus isolates obtained, 45 (11%) were VRSA. The most common VRSA isolates were male patients (57.8%) with the mean age being 41-60 years old (35.6%). The treatment ward with the most VRSA isolates were from surgical ward (20%), and internal medicine ward (15.6%) whereas the? VRSA isolates from outpatient record were from surgical? department (8.9%), dermatology department and ENT department (2.2%). The most common specimens were blood (28.9%), pus (26.7%), and sputum (20.0%). The highest prevalence of VRSA was found in the use of ? 3 invasive medical devices and from clinical outcome was found 11 (24.4%) of the patients died. Linezolid, Quinupristin / Dalfopristin, Tigecycline, and Nitrofurantoin as a therapeutic option have > 90% sensitivity. Identification of ?VRSA in Dr. Wahidin Sudirohusodo Hospital Makassar emphasized the need for? immediate action by infection control division, especially in related units to prevent the transmission.
RESISTENSI TERHADAP METHICILLIN (METHICILLIN RESISTANT) STAPHYLOCOCCUS AUREUS DI INSTALASI RAWAT INAP ., Wildana; Sennang, Nurhayana; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1047

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. MRSA infection typically aggravatesthe patient condition. MRSA infection increases morbidity and mortality. The study was aimed to find out the MRSA occurrence inDr. Wahidin Sudirohusodo Hospital Makassar patients during July 2008?June 2009. A retrospective study was performed using datafrom the medical records including the results of culture and antimicrobial susceptibility test in Dr. Wahidin Sudirohusodo HospitalMakassar. Among 1082 results of the culture test, 5.2% were identified as Staphylococcus aureus, consist of 51.8% MSSA (MethicillinSensitive Staphylococcus aureus) and 48.2% MRSA. Most of the MRSA patients were treated in orthopaedic surgery (30%), internal(22%), and paediatric (19%) wards. Based on the clinical conditions, most of the patients were in post surgery care (44.4%), pneumonia(18.5%), and diabetic foot (7.5%). All of the MRSA isolates were multiresistant (resistant to three or more antimicrobials) but 96%remain sensitive to vancomycin. It was concluded that most of MRSA patients were staying in the orthopaedic surgery ward. Based onthis clinical condition, most of the patients were in the post surgery care. All of the MRSA isolates were multiresistant, but most of themremain sensitive to vancomycin.
RERATA VOLUME TROMBOSIT, HITUNG LEUKOSIT DAN TROMBOSIT DI APENDISITIS AKUT Festiawan, Jayadi; Sennang, Nurhayana; Samad, Ibrahim Abdul
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1075

Abstract

The diagnosis of acute appendicitis and its complications still depends on the radiological examination and laparoscopy techniques.The aim of this study was to know the Mean Platelet Volume (MPV), WBC and platelet value in the diagnosis of acute appendicitis,associated with the incidence of existing complications. This research was carried out by a retrospective study by taking secondary datafrom the medical records, including the results of routine blood tests of acute appendicitis patients with and without complications atthe Dr. Wahidin Sudirohusodo Hospital between May 2011? May 2012. The data obtained in the study were MPV, White Blood Cell(WBC) and platelet count which were then analyzed using independent T test. The results obtained from 44 samples were that MPVvalues showed a significant difference (p=0.02), while WBC (p=0.12) and platelet count (p=0.13) were statistically not significantlydifferent between patients with and without complications. In conclusion, it was found that acute appendicitis with complications hadhigher MPV average values than those without complications and although there were significant differences between them, these werestill within the normal values.
DIAGNOSTIC VALUE OF URIC ACID IN PLEURAL EFFUSION Hartono, Saraswati Wulandari; Sennang, Nurhayana; Mangarengi, Fitriani
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 2 (2018)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i2.1314

Abstract

Pleural effusion is excessive pleural fluid accumulation in the aimed pleural cavity, are categorized into exudate and transudate. Light?s criteria (1972) has become a classic criteria to distinguish pleural effusion types. Other criteria were evaluated such as pleural fluid uric acid. The aimed of this study was to analyze the difference of uric acid level between transudate and exudate and to compare it with Light?s criteria. A cross-sectional study was conducted in Clinical Pathology Laboratory of the Dr. Wahidin Sudirohusodo Hospital Makassar in September 2016. Uric acid level of pleural effusion samples was measured by ABX Pentra 400. Statistical analysis used Mann-Whitney U test, significance was indicated if p<0.05. Cut-off, sensitivity and specificity of uric acid used ROC curve. Total subjects were 56 patients, mean age 49.54+13.63 years-old, 31 males (55.4%) and 25 females (44.6%). Most cases were exudative effusion (58.9%) with 19 malignancy cases (33.9%). Uric acid level median was 6.6 mg/dL (3.24-17.50 mg/dL) higher in transudate than exudate 5.01 mg/dL (0.6-9.40 mg/dL) (p=0.001). The cut-off point for pleural fluid uric acid was 5.845 mg/dL, with a sensitivity of 78.3% and specificity of 66.7%. Sensitivity and specificity of Light?s criteria was 97% and 60.9%. There was a significant difference between pleural fluid uric acid level in transudate and exudate, higher in transudate than exudate. Light?s criteria were higher in sensitivity than uric acid, but lower in specificity. Further research is needed with better sampling method to reduce bias.?
MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII (MDRAB) PREVALENCE AND CHARACTERISTICS IN DR.WAHIDIN SUDIROHUSODO GENERAL HOSPITAL OF MAKASSAR Tungadi, Dewi; Sennang, Nurhayana; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1363

Abstract

BackgroundMultidrug-resistant Acinetobacter baumannii (MDRAB) is a strain of Acinetobacter baumannii which is resistant to three or more classes of antibiotics. As prevalence of MDRAB increases, the antibiotics of choice become limited. Identification of MDRAB is required to manage and control infection.MethodThis was a retrospective study, conducted in Dr. Wahidin Sudirohusodo General Hospital of Makassar, dated from January to December 2016. Bacterial identification and antimicrobial susceptibility testing (AST) were performed using VITEK 2. The patient data were obtained from electronic medical records.Results and DiscussionA total of 323 Acinetobacter baumannii isolates were obtained, consisted of 188 isolates in January-June 2016 and 36 of which was MDRAB (19.15%) with the average length-of-stay 33 days; and 135 isolates in July-December 2016 and 31 of which was MDRAB (22.96%) with the average length-of-stay 27 days. MDRAB was mostly discovered from patients using 3 or more medical devices and on single antibiotic therapy. MDRAB isolates were mostly obtained in sputum and pus specimens, and majority of patients had respiratory diseases. The result of AST showed 100% and 96% susceptibility to Polymyxin B; 71.43% and 54.84% susceptibility to Amikacin; 66.67% and 50% susceptibility to Trimethoprim/Sulfamethoxazole in January-June and July-December 2016, respectively.Conclusion and SuggestionsThe prevalence of MDRAB in our hospital in 2016 was high, suggesting the needs to improve hospital infection prevention and control. Polymyxin B, Amikacin, and Trimethoprim/Sulfamethoxazole are the antibiotics of choice to treat MDRAB.
MYCOBACTERIUM TUBERCULOSIS DAN PCR Widaningsih, Yuyun; Amin, Ismawati; Sennang, Nurhayana; Bahrun, Uleng; Arif, Mansyur
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.756

Abstract

TB?s coinfection in HIV and AIDS is on the increase together with the high mortality rate. The disseminated TB infection (in blood) of?HIV and AIDS patients in Africa (2005) is about 10?25%. The aim of this study was to identify M.tb disseminated infection in HIV and AIDS?patients. In this study was done in six HIV and AIDS patients suffering from suspected TB co infection. The sputum was tested using Fast Acid?Bacilli and culture (using LJ and MGIT media) and DST. The identification of M.tb in blood used PCR with IS6110 primer (123 bp). The?study was done at Dr. Wahidin Sudirohusodo Hospital, Labuang Baji Hospital, NEHCRI and at the Biomolecular Laboratory of the Medical?Faculty, Hasanuddin University. From the six patients, four of them had CD4<50 sel/?L, two patients had FAB positive, three patients had?positive LJ culture on day 41?47, three patients had positive MGIT culture on day 9?47 and four patients had positive PCR. In this study?there was one case of negative FAB but LJ, MGIT and PCR were positive. There were two cases with positive FAB, culture and PCR, one case?with negative FAB and culture but positive PCR. The M.tb disseminated infection was 66.7% in six HIV and AIDS patients.
MALARIA KONGENITAL S, Sri Wahyunie; Sennang, Nurhayana; Daud, D.; Arif, Mansyur
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 21, No 2 (2015)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v21i2.1109

Abstract

Congenital Malaria is an infectious disease caused by the malaria parasite that is transmitted from mother to child through theplacenta during pregnancy or at delivery. Clinical manifestations which may arise due to Plasmodium infection are: the irritability,fever, anaemia, jaundice and hepatosplenomegaly. The incidence of congenital malaria according to the National Basic Health Research2010 is only about 0.3%. Forty two days old male baby with the main complaints fever and pale since he was three (3) weeks old. Fromthe physical examination the reviewer found anaemia, jaundice and splenomegaly. Plasmodium vivax was detected by serologic andmicroscopic examination. From the pregnancy history of mother the reviewer found that at the age of seven (7) months of pregnancyshe suffered from malaria caused by Plasmodium vivax the same as the type of Plasmodium infected the baby. The baby was born innon malaria endemic area which enhanced the diagnosis of congenital malaria of this patient. The patient was fully recovered aftertreated with dehydroartemisin piperaquin and the reviewer reported one case of congenital malaria, forty twodays old male baby. Thediagnosis was made based on the malaria history of mother at seven (7) month of pregnancy, the serologic and microscopic examinationfrom the patient blood and the baby was born in a non malaria endemic area. The prognosis of patient with congenital malaria causedby Plasmodium vivax generally was good. The clinical condition was improved and fully recovered after treated with dehydro-artemisinpiperaquin.
BAKTERI AEROB PATOGEN DAN UJI KEPEKAAN ANTIMIKROBA DI RUANGAN PERAWATAN PENYAKIT DALAM Raya, Fedelia; Sennang, Nurhayana; Aprianti, Suci
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 18, No 3 (2012)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v18i3.374

Abstract

Pathogenic bacteria are the major causes of airborne infection at the hospital ward. Nosocomial infection can occur at the opened as well?as at the closed room. Nosocomial infection influences the morbidity and mortality in the hospital and need an extra attention, because of?the increased number of hospital patients, micro organism mutation and increased of bacteria resistance to antibiotics. The aim of this study?was to quantify the number of aerobic bacteria, and to know the pathogenic bacteria identification and its determination on the susceptibility?of the antimicrobial problems at the internal medicine ward. This research was carried by a cross sectional study, which performed by?collecting air samples in eight internal medicine ward of Dr. Wahidin Sudirohusodo Hospital using Microbiology Air Sampler 100 (MAS?100). The bacterial identification and the antimicrobial susceptibility test (AST) were conducted at the Balai Besar Laboratorium Kesehatan?(July to August 2009). In this study were found the numbers of bacteria colonies about 580?6040 CFU/m3. The pathogenic bacteria that?identified were Acinetobacter calcoaceticus, Staphylococcus saprohpyticus, Enterobacter hafniae and Stomatococcus mucilaginosus that were?sensitive to Amikasin, Gentamicyn, Azitromycin and Norfloxacyn but resistant to Ampicillin. The number of bacterial colonies exceeded the?established number standard by Decree of the Indonesian Health Minister. The pathogenic bacteria showed the most sensitive result of AST?were Acinetobacter calcoaceticus, Enterobacter hafniae, Stomatococcus mucilaginosus and Staphylococcus saprohpyticus.
BAKTERI AEROB DAN UJI KEPEKAAN ANTIMIKROBA Zuhriah, Erviani; Sennang, Nurhayana; ER, Darmawaty
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.385

Abstract

Nosocomial infection is an infection acquired in hospitals, which occur more frequently in poor and developing countries such as?Indonesia, one percent of the related cases leads to death. The occurrence of nosocomial infection causes the lengthening of hospitalization?and increased risk of disease transmission. According to Permenkes No. 1204/Menkes/SK/X/2004, the Emergency Room is one of the?high-risk areas. The objectives of this research were to quantify the number of aerobic bacteria, to know the identification method of?the pathogenic bacteria and to determine the antimicrobial sensitivity pattern in the emergency installation. A cross sectional study?was performed by collecting air samples at eleven treatment rooms of the Emergency installation in dr. Wahidin Sudirohusodo Hospital?Makassar by using Microbiology Air Sampler 100 (MAS 100). The bacterial identification and antimicrobial susceptibility tests (AST)?were conducted at the Balai Besar Laboratorium Kesehatan Makassar (July to September 2009). The number of bacterial colonies were?288?6570 CFU/m3. Pathogenic bacteria identified in the study were Stomatococcus mucilaginous, Staphylococcus haemolyticus and?Acinetobacter calcoaceticus, which were still sensitive to tetracycline and resistant to ceftazidim and kanamycin.The number of bacterial?colonies exceeded the standard number established by the Decree of Indonesian Health Minister
IDENTIFIKASI BAKTERI AEROB GRAM NEGATIF DAN GRAM POSITIF MENGGUNAKAN METODE KONVENSIONAL DAN OTOMATIK Tauran, Patricia M.; Handayani, Irda; Sennang, Nurhayana
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 2 (2013)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i2.1065

Abstract

Choosing the method of bacteria identification is crucial to obtain accurate and quick results. This study will analyze the identificationresults of Gram negative and Gram positive from aerobic bacteria by examination using conventional and automatic methods at Dr.Wahidin Sudirohusodo Hospital Laboratory. A total of 85 samples consisting of 66 Gram negative bacteria and 19 Gram positive bacteriawere identified using conventional and automated methods. In this study, there was some correspondent identification result betweenthe conventional as well as the automated methods, namely 31.5% for Gram negative bacteria and 30.8% for Gram positive bacteria.However, the non-correspondent identification result between conventional and automated methods was found greater, namely, 68.5%for Gram negative bacteria and 69.2% for Gram positive bacteria. The non-correspondent identification result was due to the developmentof bacterial taxonomy and the differences of numbers and types of the biochemical tests between conventional and automatic methods.Bacteria identification using automated method is more accurate and faster than the conventional method, so it is recommended usingthis particularly for the laboratory and educational referral center.