Benny Rusli, Benny
Bagian Ilmu Patologi Klinik FK UNHAS–BLU RS DR Wahidin Sudirohusodo, Makassar

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Journal : Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)

VANCOMYCIN RESISTANT STAPHYLOCOCCUS AUREUS (VRSA) IN DR. WAHIDIN SUDIROHUSODO HOSPITAL MAKASSAR Ahmad, Fatmawaty; Sennang, Nurhayana; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1385

Abstract

Vancomycin Resistant Staphylococcus aureus (VRSA) refers to bacteria that have complete resistance to Vancomycin. The increasing prevalence of VRSA can be a significant clinical problem with the limited therapeutic options. Therefore, the identification of? VRSA is necessary to improve the management of antibiotic therapy, infection control and to prevent the occurrence of wider resistance. This retrospective descriptive study used secondary data of antibiotic susceptibility test on Staphylococcus aureus identified using VITEK 2 and patient medical records from January 2015 to December 2016. The data is processed using SPSS. Of 387 Staphylococcus aureus isolates obtained, 45 (11%) were VRSA. The most common VRSA isolates were male patients (57.8%) with the mean age being 41-60 years old (35.6%). The treatment ward with the most VRSA isolates were from surgical ward (20%), and internal medicine ward (15.6%) whereas the? VRSA isolates from outpatient record were from surgical? department (8.9%), dermatology department and ENT department (2.2%). The most common specimens were blood (28.9%), pus (26.7%), and sputum (20.0%). The highest prevalence of VRSA was found in the use of ? 3 invasive medical devices and from clinical outcome was found 11 (24.4%) of the patients died. Linezolid, Quinupristin / Dalfopristin, Tigecycline, and Nitrofurantoin as a therapeutic option have > 90% sensitivity. Identification of ?VRSA in Dr. Wahidin Sudirohusodo Hospital Makassar emphasized the need for? immediate action by infection control division, especially in related units to prevent the transmission.
MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII (MDRAB) PREVALENCE AND CHARACTERISTICS IN DR.WAHIDIN SUDIROHUSODO GENERAL HOSPITAL OF MAKASSAR Tungadi, Dewi; Sennang, Nurhayana; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1363

Abstract

BackgroundMultidrug-resistant Acinetobacter baumannii (MDRAB) is a strain of Acinetobacter baumannii which is resistant to three or more classes of antibiotics. As prevalence of MDRAB increases, the antibiotics of choice become limited. Identification of MDRAB is required to manage and control infection.MethodThis was a retrospective study, conducted in Dr. Wahidin Sudirohusodo General Hospital of Makassar, dated from January to December 2016. Bacterial identification and antimicrobial susceptibility testing (AST) were performed using VITEK 2. The patient data were obtained from electronic medical records.Results and DiscussionA total of 323 Acinetobacter baumannii isolates were obtained, consisted of 188 isolates in January-June 2016 and 36 of which was MDRAB (19.15%) with the average length-of-stay 33 days; and 135 isolates in July-December 2016 and 31 of which was MDRAB (22.96%) with the average length-of-stay 27 days. MDRAB was mostly discovered from patients using 3 or more medical devices and on single antibiotic therapy. MDRAB isolates were mostly obtained in sputum and pus specimens, and majority of patients had respiratory diseases. The result of AST showed 100% and 96% susceptibility to Polymyxin B; 71.43% and 54.84% susceptibility to Amikacin; 66.67% and 50% susceptibility to Trimethoprim/Sulfamethoxazole in January-June and July-December 2016, respectively.Conclusion and SuggestionsThe prevalence of MDRAB in our hospital in 2016 was high, suggesting the needs to improve hospital infection prevention and control. Polymyxin B, Amikacin, and Trimethoprim/Sulfamethoxazole are the antibiotics of choice to treat MDRAB.
RESISTENSI TERHADAP METHICILLIN (METHICILLIN RESISTANT) STAPHYLOCOCCUS AUREUS DI INSTALASI RAWAT INAP ., Wildana; Sennang, Nurhayana; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 17, No 1 (2010)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v17i1.1047

Abstract

Methicillin Resistant Staphylococcus aureus (MRSA) is a major nosocomial pathogen worldwide. MRSA infection typically aggravatesthe patient condition. MRSA infection increases morbidity and mortality. The study was aimed to find out the MRSA occurrence inDr. Wahidin Sudirohusodo Hospital Makassar patients during July 2008?June 2009. A retrospective study was performed using datafrom the medical records including the results of culture and antimicrobial susceptibility test in Dr. Wahidin Sudirohusodo HospitalMakassar. Among 1082 results of the culture test, 5.2% were identified as Staphylococcus aureus, consist of 51.8% MSSA (MethicillinSensitive Staphylococcus aureus) and 48.2% MRSA. Most of the MRSA patients were treated in orthopaedic surgery (30%), internal(22%), and paediatric (19%) wards. Based on the clinical conditions, most of the patients were in post surgery care (44.4%), pneumonia(18.5%), and diabetic foot (7.5%). All of the MRSA isolates were multiresistant (resistant to three or more antimicrobials) but 96%remain sensitive to vancomycin. It was concluded that most of MRSA patients were staying in the orthopaedic surgery ward. Based onthis clinical condition, most of the patients were in the post surgery care. All of the MRSA isolates were multiresistant, but most of themremain sensitive to vancomycin.
BAKTERI AEROB DAN BAKTERI PENYEBAB PENYAKIT DI neonATAL InTenSIve CARe unIT Suriyanti, Suriyanti; Handayani, Irda; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.464

Abstract

Infectious diseases are still the main problem due to the potency of life threatening for neonates. Patients in the Neonatal Intensive?Care Unit (NICU) are at risk for nosocomial infections resulting from the exposure to invasive procedures and medical equipments such?as ventilator, catheter, Continuous Positive Airway Pressure (CPAP), incubator and antibiotics treatment. This study was aimed to know?the related condition in NICU Dr. Wahidin Sudirohusodo Hospital Makassar by identification and determining the pathogenic bacteria?colony counts of incubator and CPAP. The study design was a cross sectional method, by using incubator and CPAP as sample instruments,?both equipments were sterilized at the NICU. The samples were collected and evaluated in July 2011. Samples were collected by usinga swab which was wiped to the instrument and put into Buffer Phosphate Saline (BPS); then diluted and put into Plate Count Agar?(PCA) media for culture. The result of this study showed that there were no bacteria colony in the incubator, while in CPAP Acinetobacter?calcoaceticus with bacteria colony count approximately 3.4?102 CFU/m3 were found. The researchers concluded that the incubator was?sterile to bacteria, however in the CPAP bacteria from the strain of Acinetobacter calcoaceticus were found
EVALUASI AKTIVITAS TRANSAMINASE, DAN KADAR BILIRUBIN PADA PENDERITA VIRUS HEPATITIS B DAN C ., Yosepin; Rusli, Benny; ., Hardjoeno
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 1 (2009)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i1.996

Abstract

Hepatitis virus replicates only in hepatocyte. In hepatocyte injury, transaminase enzymes which normally intracellularly will transform to the blood circulation. And there are variation of increasing transaminase activities in HBV and HCV. To evaluatetransaminase activities, and bilirubin level in patients with HBV and HCV. Study design was cross sectional of 76 patients with HBVand HCV, data from medical record at Wahidin Sudirohusodo Hospital, Makassar, from January 2006 until July 2008 period. Datawas analyzed with Mann Whitney test using SPSS. windows versi on 12. of 76 patients with HBV and HCV were found 59 Men and 17women, age 15?72 years old. In there were no statistical significance between transaminase activites, bilirubin direct,indirect (p > 0.05),but there was significance difference of total bilirubin between HBV and HCV (p < 0.05). There were no statistical difference of AST,ALT, bilirubin direct and bilirubin indirect between HBV and HCV. Injury hepatocelluler because of HBV and HCV can not be identifiedjust by transaminase activities and bilirubin level, but there are other factor that influence.
PATTERN OF BACTERIA AND ITS ANTIBIOTIC SENSITIVITY IN SEPSIS PATIENTS (Pola Kuman dan Kepekaan terhadap Antibiotik bagi Pasien Sepsis) Wahyuni, Wahyuni; Nurahmi, Nurahmi; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1189

Abstract

Sepsis merupakan masalah serius karena berkebahayaan kematian yang tinggi. Penggunaan antibiotik profilaksis telah menjaditata langkah baku untuk penanganan sepsis, tetapi jika pola kuman dan kepekaan antibiotik tidak diketahui, maka akan memperbesarkemungkinan resistensi kuman terjadi yang selanjutnya akan menyebabkan peningkatan angka infeksi. Penelitian ini bertujuanmengetahui pola kuman dan kepekaannya terhadap antibiotik di pasien sepsis di RS Dr. Wahidin Sudirohusodo Makassar masa waktuJanuari 2009?Desember 2010. Metode penelitian yang digunakan adalah observational dengan desain potong lintang. Data penelitiandiambil dari hasil kultur dan kepekaan terhadap antibiotik yang tertulis di rekam medis pasien sepsis di RS Dr. Wahidin SudirohusodoMakassar masa waktu Januari 2009?Desember 2010. Data yang dikumpulkan kemudian dianalisis menggunakan metode statistiksebaran kekerapan. Telitian menunjukkan umur pasien sepsis terbanyak adalah di atas 40 tahun. Infeksi lebih banyak disebabkan olehbakteri negatif Gram (90,48%). Urutan kuman terbanyak adalah Alkaligenes faecalis (30,16%), Escherichia coli (12,70%), Acinetobactercalcoaceticus (12,70%) dan Staphylococcus aureus (7,94%). Uji kepekaan terhadap 39 jenis antimikroba, terdapat 12 antimikroba yangmemperlihatkan presentase resistensi yang tinggi (75% ke atas). Antimikroba yang paling peka yaitu Nitrofurantoin (83,33%). Dapatdisimpulkan bahwa bakteri yang terbanyak ditemukan di sepsis adalah Alkaligenes faecalis, Escherichia coli, Acinetobacter calcoaciticusdan Staphylococcus aureus. Antimikroba yang masih peka adalah Nitrofurantoin. Disarankan untuk dilakukan penelitian pola kumandan kepekaan terhadap antibiotik secara berkala.
POLA KETAHANAN (RESISTEN) DAN KEPEKAAN (SENSITIVITAS) KUMAN TERHADAP ANTIMIKROBA Tallulembang, Y F; Sennang, Nurhayana; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 16, No 2 (2010)
Publisher : PERHIMPUNAN DOKTER SPESIALIS PATOLOGI KLINIK INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v16i2.958

Abstract

In the developing countries including Indonesia, there still have problems with infectious diseases. Generally the hospitalized patientsin the Surgery section through have antimicrobial therapy. The treatment is intended to prevent nosocomial infection that usually foundin overnight treatment patients at the department of surgery. This study is carried out to know the pattern of micro-organism?s resistanceand sensitivity against various antimicrobial at department of surgery, Dr. Wahidin Sudirohusodo Hospital Makassar, January?June2008. A descriptive study of retrospectively collective data was carried out on 160 specimens by sensitivity test at the department ofsurgery, Dr. Wahidin Sudirohusodo Makassar Hospital, started from January up to June 2008. The five common bacterial found in thisstudy where Staphylococcus epidermidis, Klebsiella pneumonia, Enterobacter agglomerans, Staphylococcus saprophytic and Escherichiacoli. The sensitive antimicrobial drugs found where Vancomicin, Sulbactam/Cepoperazone, Cefepim, Gentamicin, Ceftazidim, Novobiocin,followed by the resistance antimicrobial were Methicillin, Tetracycline, Cefuroxim, Erythromycin, and Doxyciclin. The most bacterial typefound was Klebsiella pneumoniae, the most sensitive antimicrobial was Vancomicin and the most resistances were Methicillin.
KEPEKAAN ANTIMIKROBA KULTUR DARAH DI SEPSIS NEONATAL Noor, Tajuddin; Sennang, Nurhayana; Rusli, Benny
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 1 (2012)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i1.388

Abstract

Sepsis was one of the morbidity and mortality causes in neonatal. The diagnosis and treatment requires the bacterial identification and selection of sensitive antimicrobials. The aim of this study was to know the bacterial pattern and antimicrobial sensitivity of blood culture in the suspected neonatal sepsis patients who were treated at NICU in Dr.Wahidin Sudirohusodo Hospital Makassar. A retrospective study was conducted with secondary data from the culture and antimicrobial susceptibility test between the period of June 2010?July 2011. In this present study was found that from the total 91 blood culture isolates, bacteria Gram-negative group was 85.7% and Grampositive was 14.3% and the isolate encountered in order of frequency were Alkali genes faecalis 50.5%, Klebsiella pneumonia 25.3%, and Staphylococcus epidermidis 9.9%. In the Gram-negatives group, the isolate often encountered were Alkali genes faecalis 59.0%, Klebsiella pneumonia 29.4% and Enterobacter spp 6.4% while in the Gram-positive group were found Staphylococcus epidermidis 69.2% and Staphylococcus saprophytic 23.1%. The more sensitive antimicrobal that belong to Gram-negative group were Meropenem 94.4%, Levofloxacin 92.1%, and Ceftazidime 77.0% while the more resistant were Ampicillin 94.6%, Gentamycin 89.1% and Cefuroxime 82.7%. The more sensitive antimicrobal that belong to Gram-positive group were Vancomycin and Chloramphenicol 91.7% and Novobiosin 76.9% while the more resistant were Gentamycin and Ceftriaxone 100.0% and Amoxicillin 91.7%. Based on this study it can be concluded that Gram-negative aerobe bacteria was more common than the Gram-positive one. Meropenem, Levofloxacin and Ceftazidime antimicrobal were high sensitive to Gram-negative while Vancomycin, Chloramphenicol, and Novobiocin were high sensitive to Gram-positive. The resistance of Ampicillin and Gentamycin were found in both bacterially groups of sepsis suspected neonatal patients in NICU