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GROWTH RESPONSE TEST ON POTENTIAL INDIGENOUS BACTERIA TO DEGRADE NAPHTHALENE AND PHENANTHRENE ON VARIOUS PH Hardianti, Alfi Rizca; Lusiana, Helga; Sawitri, Dita Widayanti
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0201932105264-0-00

Abstract

This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH. The isolates used in this study were Isolate A, Isolate E, and Isolate F as a result of isolation from oil sludge in Dumai. Based on the results of the growth response test on various concentration in previous studies, we chose E isolates to be tested for its degradation ability. The bacterial growth response data was obtained based on OD measurements every 24 hours using a spectrophotometer and TPC at 72 hours using Nutrient Agar media. Based on these data, we determined the optimum pH for indigenous bacterial growth potential to degrade naphthalene or phenanthrene. In all variations of pH, bacterial isolate E experienced growth. This shows that bacterium E can use naphthalene and fenantren at normal pH range (pH 5-9).
EKSPLORASI JAMUR ALKALOTOLERAN DARI DESA SUKOLILO BARAT, KECAMATAN LABANG, KABUPATEN BANGKALAN, MADURA, JAWA TIMUR Lusiana, Helga; Rukmi, MG Isworo; Raharjo, Budi
Bioma : Berkala Ilmiah Biologi Vol. 16, No.1, Tahun 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3031.181 KB) | DOI: 10.14710/bioma.16.1.10-17

Abstract

Mold exploration from limestone hills West Sukolilo  village, District Labang, Bangkalan, Madura, East Java,  conducted to found the  alkalotolerant mold isolates and investigate their  amylolytic, proteolytic, and cellulolytic activity. Mold isolation were done using spread plate and dilution method  on  PDA and CMA at pH 8.0, 9.0, and 10.0. Twenty-seven mold isolates have been found consist of  four genus  i.e. Aspergillus, Fusarium, Curvularia, and Trichoderma. The result of amylolytic test  showed Trichoderma longibrachiatum (IMD-26) have the  highest activity at pH 8, while  Fusarium sp 2 (IMD-24) showed the highest activity at pH 9. Aspergillus flavus (IMD-7) showed highest proteolytic activity at pH 8, while Hyphomycete (IMD-27) showed the highest activity at pH 9. The highest cellulolytic activity at pH 8 showed by Trichoderma harzianum (IMD-25) and pH 9 by Fusarium sporotrichoides (IMD-20). Keyword : alkalotoleran mold, amylolytic, proteolytic, cellulolytic.
THE POTENTIAL MICROBE AGENT TO DEGRADE NAPHTALENE AND PHENANTHRENE IN DUMAI OIL SLUDGE : A GROWTH RESPONSE TEST Hardianti, Alfi Rizca; Sawitri, Dita Widiyanti; Lusiana, Helga
The Journal of Experimental Life Science Vol 9, No 3 (2019)
Publisher : Graduate School, University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2019.009.03.07

Abstract

This study aims to find the most appropriate indigenous bacteria as Naphthalene and Phenanthrene degrading agents carried out in the growth response test. This research is very important to find the best bacterial agent, which in the future can reduce the impacts of oil pollution, as well as from the presence of oil sludgeBacterial cultures added in the media as much as 5% (v / v), which had previously been measured OD660nm = 0.5. The growth response test was carried out by growing 20 mL of bacteria in AMS media which was added with PAH substrate, naphthalene or Phenanthrene in various concentrations: 200 ppm, 500 ppm, 800 ppm, and 1000 ppm. In the naphthalene substrate with 24-hour incubation, the best treatment was in isolates A with a concentration of 200 ppm. In the phenanthrene substrate 24 hours incubation, the best treatment was in isolate A at 200 ppm, and at 48 hours incubation. Bacterial isolates F in 24-hour incubation increased growth in all treatments and controls. The bacterial E isolate on the phenanthrene substrate at 24-hour incubation has increased growth, which occurs until the incubation time is 48 hours, both in the control and phenanthrene substrate. E Isolate is tolerant of increasing substrate concentrations up to 1000 ppm, both naphthalene and phenanthrene and has the best growth ability. It can be concluded that isolate E has the best growth ability and is tolerant of increasing substrate concentrations up to 1000 ppm. E isolates were designated as the best isolates of naphthalene and phenanthrene degradation.Keywords: Dumai, Growth Response, Naphthalene, Phenanthrene