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UJI BRINE SHRIMP LETHALITY TEST (BSLT) UMBI BAWANG TIWAI (ELEUTHERINE BULBOSA (MILL.) URB) DAN UJI TOKSISITAS AKUT FRAKSI AKTIF Lestari, Dwi; Kartika, Rudi; Marliana, Eva
Jurnal Riset Kefarmasian Indonesia Vol 1 No 1 (2019): Jurnal Riset Kefarmasian Indonesia
Publisher : APDFI (Asosiasi Pendidikan Diploma Farmasi Indonesia)

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Abstract

Eleutherine bulbosa (Mill.) Urb come from the Iridaceae family, this species contains secondary metabolites in the form of flavonoids and quinones. This research method includes Brine Shrimp Lethality Test (BSLT) toxicity using Artemia salina Leach larvae to determine the LC50 value. This study used experimental methods pharmacologically using a completely randomized design in the same direction pattern in the selection of test animals, 25 animals used were white mice divided into 5 groups with 5 mice per group, with 4 hours observed to find out toxic symptoms and continued observation every 24 hours to see death. Testing of BSLT results showed that ethanol extract had LC50 toxicity = 66.68 ppm (very toxic category), n-hexane fraction had LC50 toxicity = 47.64 ppm (very toxic category), chloroform fraction had LC50 toxicity = 295.1 ppm ( toxic category), and the water fraction has a toxicity of LC50 = 194.54 ppm (very toxic category). The chloroform fraction is an active fraction. Acute toxicity test LD50 value according to the calculation method of Miller Tainter Probit (187,499 mg/KgBB), the method of calculation of Thompson Weil (182,810 mg/KgBB), how to calculate Indonesian Pharmacopoeia (187,068 mg/KgBB). Based on the three calculations, the LD50 value is in the medium category (50-500 mg/kgBB).
PENGARUH PENAMBAHAN IKAN KEMBUNG (RASTRELLIGER KANAGURTA L.) DALAM PAKAN TERHADAP PENURUNAN KADAR TOTAL KOLESTEROL DALAM DARAH MENCIT (MUS MUSCULUS) JANTAN YANG HIPERKOLESTEROLEMIA Fitriana, Mila; Kartika, Rudi; Panggabean, Aman Sentosa
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2017
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Fish is a source of omega-3 which is good for our health. This study used one type of fish that contains high omega-3 (Rastrelliger kanagurta L.). This fish as s feed against the decrease in total blood cholesterol levels in mice (Mus musculus) are hypercholesterolemia. There are 5 treatments used in this study, i.e positive control, negative control, and mice which given the addition of bloated fish on standard feed with 3 different treatments L1 (2:1), L2 (2:2), L3 (1:2). Parameters measurement of total cholesterol level of mice is using CHOD-PAP method. The principle used in determining the total cholesterol level of mice blood cholesterol obtained is (+) 142 ; (-) 86 ; (L1) 108 ; (L2) 87 ; (L3) 74 .The result of this study showed that bloated fish (Rastrelliger kanagurta L.) used to reduce the total blood cholesterol level of male hypercholesterolemia mice (Mus musculus).   Keywords : total cholesterol, bloated fish, omega-3, Hypercholesterolemia, male mice
peer review Jurnal Karya Ilmiah 2014 Kartika, Rudi
REPOSITORY KIMIA Dr. Rudi Kartika, M.Si
Publisher : REPOSITORY KIMIA

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Abstract

Structure characterization of Alkoloid Scorodocarpines  Derivatif from fruits of Sorodocarpus Borneesis Becc (Olacaceae)
UJI ANTIKANKER KULIT KAYU BAWANG HUTAN (Scorodocarpus borneensis Becc.) TERHADAP SEL LEUKEMIA L1210 Kartika, Rudi; Simanjuntak, Partomuan
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2013
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Penelitian ini bertujuan untuk memperoleh informasi farmakologi dari senyawa kimia yang terkandung pada kulit batang kayu bawang hutan (Scorodocarpus borneensis Becc) yaitu nilai aktivitas antikanker terhadap sel leukemia L1210. Hasil uji menunjukkan semua fraksi memiliki aktivitas IC50 yang cukup tinggi, dimana hasil tertinggi diperoleh dari fraksi etil asetat dengan IC50 = 25,2685 ppm. 
UJI BSLT DAN ANTI OKSIDAN EKSTRAK N-BUTANOL DAN AIR PADA RANTING TANAMAN SIRIH HUTAN (Piper aduncum .L.) Ningsih Kalauw, Syelvia Lousiana; Ilang, Yerwanto; Kartika, Rudi; Rachman, Fauzi; Simanjuntak, Partomouan
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2014
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Forest betel plant is a plant belonging to the Piperaceae tribe. Sap of plants are often used by people as a cure boils and leaves as toxicity to mosquito larvae o f Aedes aegypti L. Was tested for BSLT (Brine shrimp Lethality Test) and antioxidants in ethanol and water ext ract of the twigs of the plant betel leaves this forest, the concentration used in the test BSLT 10 , 100 and 1000 pp m and antio xidant tests with obstacle (inhibit ion)% 5, 10, 25, 50 and 100pp m. Principles used in to xicity tests of ethanol and water extracts of p lant twigs forest betel Piper aduncum .L ( family :p iperaceae) BSLT (Brine shrimp Lethality Test) determined by the method of total mortality of larvae shrimp Artemia salina Leach extract co mpounds due to the influence of natural materials with a part icular concentration and antioxidant principle is based on the color of the solution changes DPPH (1,1-di fenil-2-pikrilhidrasil) from purple to yellow due to the presence of antio xidant compounds. Where the results of research in BSLT test showed that the LC 50 value twigs of 33.88 pp m ethanol extract and water extract of 323.593 ppm. The antio xidant test IC 50 values obtained at 140.732 pp m ethanol extract and water ext ract of 438.687 pp m with a b lank absorbance by 0.813.
THE EFFECT OF MACKEREL (Scomberomorus commerson) IN THE FEED TO THE DECLINE IN BLOOD CHOLESTEROL LEVEL HYPERCHOLESTEROLEMIC MALE MICE (Mus musculus) Kartika, Rudi; Pratiwi, Djihan Ryn; -, Arifwan; Utama, Wahyu Budi; Santoso, Wahyu Teguh
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2014
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Blood cholesterol levels wh ich were higher than normal is trigger of various diseases. The objective of the research was to know “The Effect of Mackerel (Scomberomorus co mmerson) in the Feed to the Decline in Blood Cholesterol Levels in Hypercholesterolemic Male Mice (Mus musculus). The study was conducted over 40 days using completely randomized design with treatments, the positive control, negative control, and the mice were given the addition of mackerel on a standard feed with 3 different levels so grouped in L1 (2:1); L2 (1:1) and L3 (1:2). The parameters measured are total cholesterol blood serum of mice with the CHOD-PAP method. Cholesterol levels are measured with a photometer 5010 at a wavelength of 500 nm. The result in the (-) 86 mg/d L; (+) 113 mg/d L; L1 102 mg/d L; L2 86 mg/dL; and L3 82 mg/dL.
UJI AKTIVITAS ANTIOKSIDAN DAN TOKSISITAS EKSTRAK N- HEKSAN DAN ETIL ASETAT RANTING SIRIH HUTAN (Piper aduncum L.) Ilang, Yerwanto; Kalauw, Syelvia Lousiana; Kartika, Rudi; Rachman, Fauzi
PROSIDING SEMINAR KIMIA SEMINAR NASIONAL KIMIA 2014
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

The aim of this study was to determine the antio xidant and toxicity act ivities of n-hexane and ethyl acetate of Piper aduncum L. (family : Piperaceae). Antio xidant activities of the ext racts was assessed by means of DPPH free radical scavenging method used ascorbic acid as standard with IC 50 value was 4,841 μg/mL. Piper aduncum L. showed n-hexane extract demonstrated the good antioxidant activity with IC 50 value of 40,034 μg/ mL and the ethyl acetate extract is 59,838 μg/ mL. The Brine shrimp letality bioassay met od as sed to determine t e cytotoxicity activities. The LC 50 values of n-hexane and ethyl acetate extracts were 25,12 and 17,78 μg/ mL, respectively.
Isolasi dan Identifikasi Senyawa Kimia dari Ekstrak n-heksan Batang Benalu Tanaman Jeruk (Dendrophtoe pentandra (L.) Miq.) hanif, rizky maulida amalia; Kartika, Rudi; Simanjuntak, Partomuan
JURNAL KIMIA MULAWARMAN Vol 14 No 1 (2016)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Telah dilakukan penelitian isolasi dan identifikasi senyawa kimia dari ekstrak n-heksan batang benalu tanaman jeruk (Dendrophtoe pentandra (L.) Miq.). Penelitian ini dilakukan dengan metode maserasi dengan pelarut n-heksan, etil asetat, etanol dan air, uji fitokimia, uji mortalitas larva udang dengan metode BSLT (Brine Shrimp Lethality Test) serta isolasi dengan kromatografi kolom dan identifikasi dengan menggunakan spektrofotometer Fourier Transform Infra Red (FT-IR) dan Kromatografi Gas – Spektrometri Massa (KG-SM). Dari hasil uji mortalitas larva udang didapatkan bahwa ekstrak n-heksan adalah ekstrak yang paling aktif kemudian dilanjutkan pada proses isolasi dan diperoleh isolat n-heksan 3.2 yang memiliki nilai toksisitas (LC50) sebesar 92,197 ppm. Berdasarkan hasil analisis dengan spektrofotometer FT-IR dan KG-SM senyawa kimia yang diduga terdapat dalam ekstrak n-heksan batang benalu tanaman jeruk (Dendrophtoe pentandra (L.) Miq.) adalah senyawa Stigmasterol.
AKTIVITAS ANTIHIPERGLIKEMIK DARI EKSTRAK ETANOL DAN n-HEKSANA DAUN KEMBANG BULAN [Tithonia diversifolia A.Gray] PADA TIKUS PUTIH JANTAN Darmawi, Ary Rizki; Saleh, Chairul; Kartika, Rudi
JURNAL KIMIA MULAWARMAN Vol 12 No 2 (2015)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Phytochemical test and antihyperglicemic activity from ethanol extract dan n-hexane extract from kembang bulan (Tithonia diversifolia A.Gray) leaf has been carried out. Based on the test result of phytochemistry of secondary metabolites compounds from kembang bulan (Tithonia diversifolia A.Gray) leaf showed that ethanol extract fraction containing flavonoid, fenolik, alkaloid and saponin. Whereas at n-hexane extract fraction containing alkaloid, saponin and steroid. Antihyperglicemic activity test using the method of glucose tolerance where a white male rats got induced by sucrose. Measurement of blood sugar levels using glucometer and glucose test strip. From this test shows that the most active fractions to reduce blood sugar levels was ethanol extract with a dose of 77 mg/kg BB with percentage of blood sugar levels reduction in the amount of 54.15 % it was close with reduction percentage from acarbose as positive control in the amount of 55.57 %.
Pengaruh Pemberian Ekstrak Etanol Daun Kecapi (Sandoricum koetjape (Burm.f.) Merr.) Terhadap Penurunan Kadar Kolesterol Total pada Mencit Jantan (Mus musculus) Kartika, Rudi
JURNAL KIMIA MULAWARMAN Vol 13 No 2 (2016)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

The research about utilization of ethanol extract form leafkecapi (Sandoricum koetjape (Burm.f.)Merr.)has been done, it proved could decrease  total cholesterol levels in male mice (Mus musculus). In this research sample macerated with ethanol 96% and then concentrated by rotary evaporator, and gained 15.6% of yield. Based on the results of phytochemical test, secondary metabolites compound that contained in bark kecapi are alkaloid, flavonoid, triterpenoid, steroid, phenolic and saponin. On antihiperkolesterolemia activity test by using male mice, there are several treatments that negative control (-), positive control (+) and three treatment with different doses i.e. a low doses (9.5 mg/kg BB), middle doses (19 mg/kg BB) and high doses (38 mg/kg BB). The parameter measured was blood serum of male mice by using a Cholesterol Test Strips. From this test it showed that the effectiveness of decreased total cholesterol level doses of 19 mg/kg BB showed total cholesterol level under 100 mg/dL. Keywords:      Leaf kecapi (Sandoricum koetjape (Burm.f.) Merr.), Phytochemicals test, Total cholesterol levels, Cholesterol test strips.
Co-Authors -, Hamsiana Adha, Laila Sufina Aditya, Roy Alimuddin - Aman S Panggabean, Aman S Aman Sentosa Panggabean Arifwan -, Arifwan Ary Rizki Darmawi, Ary Rizki Aslamiah, Suaibatul Bohari Yusuf Bustanussalam Bustanussalam, Bustanussalam Chairul Saleh Destiana, Lumban Gaol Veronica Djihan Ryn Pratiwi, Djihan Ryn Dwi Lestari Dwi Susanto Eka Siswanto Syamsul, Eka Siswanto Erwin - Estrada, Rezky Eva Marliana, Eva Fauzi Rachman, Fauzi Firmansyah - Fitriana, Mila Fitriana, Mila Iskandar, Bayu Iskandar, Bayu Jaya, Edi Novian Jaya, Edi Novian Julianto, Faisal Reja Kalmud Nia Tekha, Kalmud Nia Laksono, Annuary Laksono, Annuary Maria Amelia Kristianingrum, Maria Amelia Marliani, Nelly Marlina, Eva Mira Wahyuni, Mira Nasution, Safridah Hannum Noor Hindryawati, Noor Nuryanti, Lila Karina Octavianus, Riyan Octavianus, Riyan Octavianus, Riyan Partomouan Simanjuntak, Partomouan Partomuan Simanjuntak Prastiwi, Mela Putra, Hendric Surya RAHMAT GUNAWAN Rico Ramadhan, Rico Rini, Eka Setia rizky maulida amalia hanif, rizky maulida amalia Ruga, Ritbey Rutdianti, Rutdianti Rutdianti, Rutdianti Sahriani, Andi Saibun Sitorus, Saibun Sari Setianingsih, Sari Setyningrum, Eka Damayanti Setyningrum, Eka Damayanti Shafarini, Eno Julia Shafarini, Eno Julia Subur P Pasaribu Sudrajat Sudrajat Sukanadi, I Putu Sulistiani, Oktaviana Dewi Syelvia Lousiana Kalauw, Syelvia Lousiana Syelvia Lousiana Ningsih Kalauw, Syelvia Lousiana Tandirerung, Edman Tandirerung, Edman Tanjung, Faisal Verani, Wulan Wahyu Budi Utama, Wahyu Budi Wahyu Teguh Santoso, Wahyu Teguh Wajdie, Farid Winni Astuti, Winni Wiratama, Sukma Yeni Agustina, Yeni Yerwanto Ilang, Yerwanto