Yuli Purwandari Kristianingrum, Yuli Purwandari
Fakultas Kedokteran Hewan, Universitas Udayana, Bali

Published : 6 Documents
Articles

Found 6 Documents
Search

PAT-2 RAPID DIAGNOSTIC TEST OF RED SEA BREAM IRIDOVIRAL DISEASE (RSIVD) IN GROUPER EPINEPHELUS SP. BASED ON SEROLOGICAL CO-AGGLUTINATION AND MOLECULAR STUDY Sulistiyono, Dwi; Amanu, Surya; Kurniasih, Kurniasih; Kristianingrum, Yuli Purwandari
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.403 KB)

Abstract

Red sea bream iridoviral disease (RSIVD) is caused by red sea bream iridovirus (RSIV), adouble stranded DNA of Icosahedral virus with a diameter of 120-240 nm [1]. RSIV is  one  of the  species  of  the Megalocytivirus,  Genus  of  the  Iridoviridae Family,  first reported to infect red sea bream (Pagrus major) fish, at Sikoku Island, Japan 1991, and since then it has been noted to cause considerable economic losses to fisheries in Singapore, Taiwan, Thailand, Korea, Philippines, Malaysia and also in Indonesia [2,3,4]. Rapid transmission with high mortality rates in fish populations infected becomes a serious threat to the aquaculture fishery business. Stained imprints or tissue sections [1], monoclonal antibody technique (MAb), Immunofluorescent Antibody Tests (IFAT) [5], Polymerase Chain Reaction (PCR) [6] Electron Microscope and Multiplex PCR [2] methods have been introduced.  Although it is very effective for detecting RSIVD in infected fish, but requires training and specialized equipment at a high cost.Co-agglutination test is a diagnostic method, used both in humans and animals in detecting bacterial or viral diseases [7], this method is fast, easy to use, and does not require special equipment. Test results from co-agglutination are easily seen macroscopically, so it is suitable if developed in RSIVD detection in the field case. This study aims to create and conduct RSIVD co-agglutination kit field tests supported by molecular studies and diagnostic analysis of the sensitivity and specificity of the accuracy and reliability of the kit. Then the test results will be compared from the pooling and individual samples.
IMMUNODIAGNOSIS INFEKSI AEROMONAS HYDROPHILA PADA IKAN Kristianingrum, Yuli Purwandari; Widyarini, Sitarina; Kurniasih, Kurniasih; Sutrisno, Bambang; Tabbu, Charles Rangga; Sugiyono, Sugiyono
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8456.913 KB) | DOI: 10.22146/jsv.38858

Abstract

Aeromonas hydrophila causes a disease that often infects fish and is known as Motile Aeromonas Septicaemia (MAS), Hemorrhagi Septisemia, Ulcer disease or Red-Sore disease. The   aims of this study were to develop polyclonal antibody of  Aeromonas hydrophila in the rabbits to   confirm the diagnosis of Aeromonas hydrophila  in the fish by immunohistochemistry staining method. Preparation of polyclonal antibodies was performed on the rabbits used to Aeromonas hydrophila bacteria that have been tested biochemically by intravenous and intraperitoneal injection. Doses of Aeromonas hydrophila  bacteria were 109 CPU/ml  of 0.5 ml at first injection, 1 ml at second injection, 2 ml at thirth injection and 3 ml at fourth injection. Blood serum collection was performed at week 5 after injection from  an  ear and intracardial vein. The result of antibody titer was 28 = 1024 which measured by   tube test. Furthermore, polyclonal   antibody was used to immunohistochemistry  staining with 400x dilution. The results of the staining showed that an immunopositive reaction in the liver, skin,lien,  gill, kidney, and heart of fish to Aeromonas hydrophila antibody. The research conclution was polyclonal antibody from rabbit can be used to accurately confirm the diagnosis of Aeromonas hydrophila  based on antigen and antibody reaction. 
GAMBARAN HISTOPATOLOGI OTAK TIKUS AKIBAT INJEKSI TRIMETYLTIN SEBAGAI MODEL PENYAKIT ALZHEIMER Kristianingrum, Yuli Purwandari; Sitarina Widyarini, Sitarina Widyarini; Kurniasih, Kurniasih; Bambang Sutrisno, Bambang Sutrisno; Charles Rangga Tabbu, Charles Rangga Tabbu Charles Rangga Tabbu; Sugiyono, Sugiyono
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.772 KB) | DOI: 10.22146/jsv.22820

Abstract

Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex.
DETEKSI BOVINE HERPESVIRUS-1 SECARA IMMUNOHISTOKIMIA PADA MEMBRAN KORIOALLANTOIS TELUR AYAM BEREMBRIO (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Kristianingrum, Yuli Purwandari; Tabbu, Charles Rangga; Sutrisno, Bambang; Widyarini, Sitarina; ., Kurniasih; Untari, Tri; Kusumawati, Asmarani
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.
STUDI IN-VIVO EKSTRAK DAUN TEH HIJAU (CAMELLIA SINENSIS) SEBAGAI ALTERNATIF ANTI BAKTERI ESCHERICIA COLI PADA AYAM BROILER Sutrisno, Bambang; Wasito, R.; Kurniasih, Kurniasih; Widyarini, Sitarina; Kristianingrum, Yuli Purwandari; Sugiyono, Sugiyono
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (441.379 KB) | DOI: 10.22146/jsv.44953

Abstract

The prevalence of colibasillosis  in chicken farms in Indonesia is very high, treatment using antibiotics is experiencing resistance, so it is necessary to look for alternatives to antibacterial. The study was aimed to determine the antibacterial effect of green tea leaf extract on broiler chickens infected with Eschericia coli by looking at the score of macroscopic lesions strengthened by histopathological examination, heterophile examination, plasma protein and fibrinogen. The research used 20 day old broilers (DOC) which were randomly divided into 4 groups, group A group B, group C and group D, each consisting of 5 DOC broilers. While maintaining ND and Gumboro vaccines on schedule like maintenance in general. At the age of 21 days all broilers in each group began to be treated as controls (Group A) without infecting E. coli and were not given 0,1g/ml  water extract of green tea leaves (Camillia sinensis). Group B, intratracheal-infected broilers with local strains of E.coli were 108 cells / ml according to 0,5  Mc Farland standard, and were not given green tea leaf extract. Group C, broilers infected by intratracheal with local strains of E. coli 108 cells / ml by 0,5 Mc Farland standard, and given to drink green tea leaf extract (Camillia sinensis) 0,1 g/ml and group D, broilers were given drinking green tea leaf extract (Camillia sinensis) 0,1g/ml. During the treatment all of chickens were given food and drink ad libitum. Fourteen days after infection of E.coli, 5 chickens in each group were collected to collect blood for heterophyll, total plasma protein (TPP) and fibrinogen. And then were euthanasied  with Mg SO4 saturated solution intravenously injection and necropsied  for gross and histpathological examination. Analysis of blood tests results were used one way of anova  (SPSS version 22 program), whereas for gross and histopathological examination with descriptive analysis. The results showed that the gross examination and histopathological organs of brolier infected with E. coli without being given a green tea extract experienced airsacculitis, pericarditis, perihepatitis and peritonitis, whereas broilers infected with E. coli and given green tea extract does not indicate the presence of inflammation. Examination of heterophile counts and blood fibrinogen levels had shown a difference (P <0.05), in broilers infected with E. coli and given green tea extracts had lower amounts of hetrophils and fibrinogen levels. While blood TPP levels were not significantly different (P> 0.05). The conclusion can be drawn, that the study of in vivo green tea extract (Camelia sinensis) 0,1g/ml has the potential to inhibit the infection of Eschericia coli bacteria in broiler chickens.
GAMBARAN HISTOPATOLOGI OTAK TIKUS AKIBAT INJEKSI TRIMETYLTIN SEBAGAI MODEL PENYAKIT ALZHEIMER Kristianingrum, Yuli Purwandari; Sitarina Widyarini, Sitarina Widyarini; Kurniasih, Kurniasih; Bambang Sutrisno, Bambang Sutrisno; Charles Rangga Tabbu, Charles Rangga Tabbu Charles Rangga Tabbu; Sugiyono, Sugiyono
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (686.772 KB) | DOI: 10.22146/jsv.22819

Abstract

Trimethyltin chloride (TMT) is an organotin compound which neurotoxic at limbus system and hippocampus in human and animal. Pathology changes that caused by the induction of TMT is a neurodegenerative disorder such as nerve cell death and cognitive impairment. This study was aimed to observe brain pathology induced by TMT with multiple doses for 14, 21 and 28 days after treatment. Twenty seven of Wistar rats, at 2 months of age with weight ranging between 200-300 grams were used and divided randomly into 3 groups (n=9). Group I were injected by trimetyiltin with a dose of 6 mg / kg, group II were injected bytrimetyltin with a dose of 8 mg / kg and group III as control without injection. Observation of brain pathology was done by euthanasia on day 14, 21 and 28 after treatment, three rats each. Cortex and hippocampus of the brainwere observed using Hematoxilin and Eosin staining (HE). All of the research procedure was done with the approval and supervision of Animal Ethics Committee LPPT UGM No. 300/KEC-LPPT/VII/2015. The observation of histopathology of the brain's neuron cells injected by trimetyltin dose of 6 mg/kg and 8 mg/kg body weight was showed increasing cell death of brain neurons in the cortex and hippocampus compared to the control group. The highest cell death was on day 14 in the hippocampus and cortex cerebral on day 21after TMT injection. The neuron cell death characterized by the shrink of brain neurons as well as colored eosinophilic cytoplasm. One way ANOVA statistical analysis showed a significant difference number of neurons cell deathbetween control and treatment groups. Based on this research, it can be concluded that the trimetyltin injection dose of 6 mg / kg and 8 mg / kg of body weight caused neuron cell death in the brain rats from fourteen day aftertreatment, especially in the hippocampus and cortex.