Articles

Found 8 Documents
Search

GENETIC PROFILE ASSESSMENT OF GIANT CLAM GENUS TRIDACNA AS A BASIS FOR RESOURCE MANAGEMENT AT WAKATOBI NATIONAL PARK WATERS Findra, Muhammad Nur; Setyobudiandi, Isdradjad; Butet, Nurlisa Alias; Solihin, Dedy Duryadi
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (801.089 KB) | DOI: 10.14710/ik.ijms.22.2.67-74

Abstract

Giant clam population has been decreased in a few years. Resource management requires information from various aspects, such as ecological, population, and other aspects. This study was aimed at assessing the genetic profile of Tridacna giant clam in Wakatobi National Park waters using Cytochrome oxidase subunit I (COI) genetic marker. Sample collection was conducted around the three main islands, i.e., Wangi-wangi, Kaledupa, and Tomia. Genetic analysis using COI gene may contribute in identifying giant clams up to the species level and showed the relationship among species. The research found 41 specific nucleotide sites for the clams. T. crocea, T. squamosa and T. maxima had 2, 15 and 24 sites, respectively. COI gene as a biological marker was able to separate groups of giant clam by species. Nucleotide variation of T. crocea from Wakatobi was the highest among other locations, so it could be used as a genetic source for translocation and domestication. Keywords: cytochrome oxidase subunit I, specific nucleotide, Tridacna, Wakatobi National Park
KEPASTIAN TAKSONOMI DAN SEBARAN BELANGKAS TACHYPLEUS TRIDENTATUS LEACH 1819 DI PERAIRAN BALIKPAPAN TIMUR Erwyansyah, .; Wardiatno, Yusli; Kurnia, Rahmat; Butet, Nurlisa Alias
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 10 No. 3 (2018): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5143.096 KB) | DOI: 10.29244/jitkt.v10i3.21917

Abstract

ABSTRAKBelangkas Tachypleus tridentatus adalah salah satu hewan bentik laut yang menghuni perairan pesisir Balikpapan Timur. Informasi tentang populasi belangkas di lokasi masih terbatas, dan IUCN secara luas mengklasifikasikannya dalam kategori data deficient. Penelitian ini bertujuan untuk menjelaskan kepastian taksonomi secara genetik dan sebaran T. tridentatus di perairan pesisir Balikpapan Timur. Belangkas diperoleh menggunakan alat tangkap nelayan setempat (dogol - sejenis pukat dan jaring insang) di daerah Teritip dan Manggar, masing-masing pada jarak sekitar 1 dan 2 mil dari garis pantai. Pengambilan belangkas dilakukan mulai Januari hingga Maret 2018 pada fase bulan berbeda. Belangkas yang tertangkap dihitung dan dicatat lokasi penangkapannya serta diamati karakter morfologinya untuk analisis sebaran dan sebagian belangkas diambil darahnya untuk analisis kepastian taksonomi dengan menggunakan penanda gen CO1. Karakter morfologi utama berupa telson yang berbentuk segitiga dan duri kecil yang banyak terdapat pada ophisthosoma, secara genetis menunjukkan bahwa jenis yang dikumpulkan adalah T. tridentatus. Sebagian besar belangkas yang ditemukan di lokasi penelitian adalah belangkas dewasa yang menyebar secara acak, dengan lebar prosoma berkisar antara 22,5 hingga 30,5 cm untuk jantan dan 28,5 hingga 37,5 cm untuk betina. Jumlah belangkas terbanyak ditemukan di daerah Teritip dan berdasarkan fase bulan terjadi pada kuarter ketiga. Penelitian ini menguatkan kepastian adanya jenis T. tridentatus di lokasi penelitian dengan ciri yang mudah dikenali serta konektivitas pergerakan T. tridentatus ke lokasi lain di sekitar perairan Balikpapan Timur. ABSTRACTThe tri-spine horseshoe crab Tachypleus tridentatus is one of marine benthic animals inhabiting East Balikpapan coastal waters. Information on horseshoe crab population in the location is still limited and IUCN broadly classifies the crab in deficient data category. This study was aimed at elucidating the taxonomic certainty genetically and distribution of T. tridentatus in coastal waters of East Balikpapan. The crabs were collected by local gear of fisherman (dogol ? a trawl like fishing-gear and gill net) in the Teritip and Manggar areas, each at a distance of 1 and 2 miles from coastline. Collecting takes place between January to March 2018 at different moon phases, location, number and morphological characters of the crabs was recorded and blood was taken from some individuals. Primary morphological characters in the form of triangular shape of telson and abundant tiny spines on ophisthosoma, genetically ascertained that the collected crab is T. tridentatus. Most of these crabs found was adult and randomly dispersed; with the width of prosoma ranged from 22.5 to 30.5 cm for males and 28.5 to 37.5 cm for females. The highest number of crab was found in the Teritip area and in the third quarter moon phase. This study reinforce the certainty of T. tridentatus in the study site with easily recognized morphological characters and the connectivity with other sites around Eastern Balikpapan waters.
DISTRIBUSI SPASIAL DAN TEMPORAL KEPITING KELAPA (BIRGUS LATRO LINN 1767) DI DAEO KABUPATEN PULAU MOROTAI, MALUKU UTARA Serosero, Rugaya; Sulistiono, .; Butet, Nurlisa Alias; Riani, Etty
Jurnal Ilmu Pertanian Indonesia Vol. 23 No. 3 (2018): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.422 KB) | DOI: 10.18343/jipi.23.3.211

Abstract

This study aims to describe the size distribution of coconut crabs spatially and temporally in Daeo, Morotai Island, North Maluku. The captching was carried out at three stations, namely in areas adjacent to residential areas (station I), steep terrain with varying vegetation (station II), and shallow areas with various vegetation and coconut trees (station III). The collections of samples were conducted using coconut bait and catching directly by hand. Their lengths of cephalotorax plus rostrum (Cp+r) and thorax (TL) and weight were measured. The habitat conditions of natural vegetation were also observed. Data collected in catch (spatial) were analyzed with non-parametric Mann- Whitney (?0.05) and temporal tests with Kruskal Wallis test (?0.05). The results showed that the total numbers of catched coconut crabs were 581 individuals consisted of 314 and 267 males and femalescoconut crabs). The weight ranges of male and female coconut crabs were 50-990 and 50-520 grams, respectively. The ranges of Cp+r on male and female coconut crabs were 43.98-114.72 mm and 43.98-90.67 mm, respectively. The sizes of the TL in male and female coconut crabs were 19.56-54.86 mm and 19.56-48.65 mm, respectively. Non-parametric Mann-Whitney test of the number of spatial coconut crab catched in station I was different from those in station II and stasion III (P<0.05). The numbers coconut crabs catched were not temporally different (P>0.05). The spatial environmental qualities were not different (P>0.05) and the temporal parameters were significantly different (P<0.05) except pH that was not significantly different (P>0.05). Station III had denser vegetation than stations I and II.
TWO NEWLY RECORDED SPECIES OF THE LOBSTER FAMILY SCYLLARIDAE (THENUS INDICUS AND SCYLLARIDES HAANII) FROM SOUTH OF JAVA, INDONESIA Wardiatno, Yusli; Hakim, Agus Alim; Mashar, Ali; Butet, Nurlisa Alias; Adrianto, Luky
HAYATI Journal of Biosciences Vol. 23 No. 3 (2016): July 2016
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1284.902 KB) | DOI: 10.4308/hjb.23.3.101

Abstract

Two species of slipper lobster, Thenus indicus Leach, 1815, and Scyllarides haanii De Haan, 1841, are reported for the first time from the coastal waters of South of Java, part of the Indian Ocean. A total of two specimens, one specimen of T. indicus from Palabuhanratu Bay and one specimen of S. haanii from Yogyakarta coastal waters, were collected in April and September 2015, respectively. Descriptions and illustrations of the morphological characteristics of the two species and their habitat are presented.
ANALISIS ORDE SUNGAI DAN DISTRIBUSI STADIA SEBAGAI DASAR PENENTUAN DAERAH PERLINDUNGAN IKAN SIDAT (ANGUILLA SPP.) DI DAS CIMANDIRI, JAWA BARAT Hakim, Agus Alim; Kamal, Mohammad Mukhlis; Butet, Nurlisa Alias; Affandi, Ridwan
Tropical Fisheries Management Journal Vol. 3 No. 1 (2019): Jurnal Pengelolaan Perikanan Tropis
Publisher : IPB PRESS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jppt.v3i1.29476

Abstract

Sungai merupakan habitat makhluk hidup dimana terjadi hubungan timbal balik antara makhluk hidup dengan lingkungannya. Sungai yang merupakan habitat ikan sidat (Anguilla spp.) harus tetap terjaga agar pertumbuhan dan perkembangan tidak terganggu serta keberadaannya tetap lestari. Ikan sidat pada stadia larva akan bermigrasi dari laut ke perairan sungai hingga hulu dan setelah dewasa akan kembali ke laut untuk memijah. Penelitian ini bertujuan untuk menganalisis orde sungai dan mengkaji distribusi stadia ikan sebagai dasar penentuan daerah perlindungan ikan sidat (Anguilla spp.) di DAS Cimandiri, Jawa Barat. Penelitian ini dilakukan pada bulan Desember 2014 dan April 2015 meliputi tracking sungai (pengamatan habitat sungai, pengamatan aktivitas penangkapan, dan wawancara), analisis orde sungai, dan penentuan distribusi tiap stadia ikan sidat. DAS Cimandiri memiliki panjang sebesar 69,5 km dengan komposisi sungai permanen (permanent) dan sungai periodik (intermittent) memiliki orde sungai dari 1 hingga 7. Terdapat 56 sungai permanen bagian DAS Cimandiri yang merupakan sungai dengan orde sungai 1. Sungai dengan orde sungai 1 merupakan bagian hulu yang kondisi habitat dan perairannya masih baik. Beberapa dari bagian sungai yang memiliki orde sungai 1 dapat ditetapkan sebagai daerah perlindungan ikan sidat (Anguilla spp.). Distribusi berdasarkan stadia menunjukkan bahwa ikan sidat memiliki komposisi stadia yang tercampur, tetapi glass eels terdistibusi hanya pada 5 km dari arah laut. Adanya perbedaan stadia dari setiap bagian sungai maka diperlukan pengelolaan yang berbeda pula pada masing-masing bagian sungai tersebut.
AUTENTIKASI SPESIES IKAN KERAPU BERDASARKAN MARKA GEN MT-COI DARI PERAIRAN PEUKAN BADA, ACEH Kamal, Mohammad Mukhlis; Hakim, Agus Alim; Butet, Nurlisa Alias; Fitrianingsih, Yulia; Astuti, Rika
Jurnal Biologi Tropis Jurnal Biologi Tropis Vol.19 No.2 Juli - Desember 2019
Publisher : Program Studi Pendidikan Biologi FKIP Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v19i2.1245

Abstract

Abstrak : Variasi bentuk dan pola pewarnaan tubuh ikan kerapu (Famili Serranidae) sangat variatif, sehingga pengenalan spesies secara morfologis sering tidak akurat. Penelitian ini bertujuan untuk mengautentikasi ikan kerapu dengan menggunakan marka gen COI. Contoh ikan kerapu yang diamati berjumlah 29 ekor yang dikumpulkan dari tiga tempat pendaratan ikan di Perairan Peukan Bada, Propinsi Aceh. Secara karakter morfologis, ikan kerapu tersebut teridentifikasi lebih dari 8 spesies. Untuk analisis DNA, sebanyak 30 mg daging sirip dari setiap ikan contoh diambil untuk dilakukan isolasi dan ekstraksi DNA, kemudian visualisasi elektroforesis dan fragmentasi DNA gen COI dengan metode PCR-sekuensing. Setelah diekstraksi, diperoleh 20 sampel DNA yang tervisualisasi dengan baik, yang dari jumlah tersebut terdapat 16 sampel dapat diamplifikasi. Hasilnya menunjukkan terdapat 6 spesies yang terautentikasi. Kelompok pertama adalah Variola albimarginata, Cephalopholis urodeta, dan C. sexmaculata dengan tingkat kemiripan ? 97%. Berikutnya C. boenak, Epinephelus merra, dan Scolopsis vosmeri tingkat kemiripannya ? 97%. Bila dibandingkan hasil autentikasi DNA, hasil penelitian menunjukkan bahwa 13 sampel atau > 80% tidak teridentifikasi dengan benar secara morfologis. Berdasarkan jarak genetik, pohon filogeni membentuk 2 clade antara Serranidae dan Nemipteridae. Hasil penelitian menunjukkan bahwa penggunaan marka gen COI sangat efektif untuk autentikasi spesies yang dapat dijadikan sebagai instrumen dalam pemanfaatan dan pengelolaan ikan kerapu.Kata kunci : kerapu, variasi morfologi, gen MT-COI, autentikasi.Abstract : The groupers (family Serranidae) show high variability both in body shapes and coloration leads to highly morphological-based misidentification. The research was aimed in autenthication of the grouper species using MT-COI gene. A total of 29 grouper fishes were collected from three fish landing sites of Peukan Bada, Aceh Province. These fishes were morphologically identified from which more than 8 species were obtained. A 30 mg of the fin meat of each sample was taken for DNA extraction, isolation, electrophoresis visualization, and DNA fragmentation of COI gene using PCR-sequenching.  There were 20 DNA samples was clearly visualized of which 16 has been proceeded for amplification. The results showed that V. albimarginata, C. urodeta, and C. sexmaculata showed ? 97% similarity, whereas C. boenak, E. merra, dan S. vosmeri with ? 97% similarity. Based on phylogenetic tree analysis there was 2 clearly different clades separating family of Serranidae and Nemipteridae. The use of MT-COI gene was effective and accurate tool in species authentication which could be used as an instrument for utilization and management of the grouper species.Keywords : groupers, morphological variation, MT-COI gene, autenthication.
KERAGAMAN MORFOMETRIK DAN GENETIK GEN COI BELUT SAWAH (MONOPTERUS ALBUS) ASAL EMPAT POPULASI DI JAWA BARAT Herdiana, Lella; Kamal, Muhammad Mukhlis; Butet, Nurlisa Alias; Affandi, Ridwan
Jurnal Ilmu Pertanian Indonesia Vol. 22 No. 3 (2017): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (391.923 KB) | DOI: 10.18343/jipi.22.3.180

Abstract

Swamp eel (Monopterus albus) is an economically important freshwater fish which has the potential to sustainable development. This species is considered as one of the taxonomically problematic spesies due to its complex characters and similar morphology to Monopterus cuchia and Monopterus javanensis that often lead to taxonomic dilemmas. Taxonomic certainty is an essential basis information in sustainable fisheries management, studies on morphometric characters, and genetic variability is a method which commonly used for species or stock identification. This study was aimed to validate the taxonomy certainty and evaluate the kinship of swamp eel from four populations (Indramayu, Subang, Tasikmalaya, and Garut distrik) in West Java. Morphometric analysis was conducted by measurement of 19 characters, whereas genetic analysis was conducted using PCR-Sequencing method of COI gene. The results from stepwise discriminant analysis showed that 7 morphometric characters ratio were powerful to classified the populations and the first two discriminant functions accounted 84.2% of the total variability. Genetic diversity indicated by alignment 14 sekuen of COI gene showed there were 11 haplotypes from 109 variable sites. Results of cluster and phylogenetic tree analysis indicated that swamp eel from four research populations were grouped into two major clusters. The first cluster consists of Indramayu and Subang population, the second cluster consists of Tasikmalaya and Garut population. This cluster is an evidence that swamp eel from West Java possesed morphometric and genetic diversity among populations and lead to assumption that at least there were two cryptic species from M. albus in West Java. 
RASIO PANJANG-LEBAR KARAPAS, POLA PERTUMBUHAN, FAKTOR KONDISI, DAN FAKTOR KONDISI RELATIF KEPITING PASIR (HIPPA ADACTYLA) DI PANTAI BERPASIR CILACAP DAN KEBUMEN Muzammil, Wahyu; Wardiatno, Yusli; Butet, Nurlisa Alias
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 1 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1161.786 KB)

Abstract

Cilacap and Kebumen are potential coastal habitat for mole crab such as Hippa adactyla. Ecosystems condition of both locations is distinct. Each organism have to develop adaptation strategy to deal with environmental variation. H. adactyla usually burry into substrate to cope with environmental stress. They have been adapted to local habitat in order to be able for survive and reproduce. However, it has not been well known whether the adaptation strategy may vary the performance of morphology. This study focused on morphological variation of mole crab H. Adactyla. The sample was carried out on August 2014. Mole crabs were caught using traditional fishing gear called ?sorok?. They were 118 individuals which where caught in Cilacap, while 102 individuals that were found in Kebumen. The result showed that H. adactyla walk vertically (backwards). These caused by carapace length and carapace width ratio. T-student analysis showed that relative growth for males were negatively allometric and isometric for female in both habitat. Mean of condition factor (K) for males in Cilacap was 0.0319 ± 0.0025 and 0.0315 ± 0.0028 in Kebumen. Those were significantly higher for females (p<0.05) in Cilacap (0.0325 ± 0.0050) and Kebumen (0.0329 ± 0.0027). Mean of relative condition factor (Kn) was higher in Cilacap for males and females. Those variations of K and Kn related to exogenous and endogenous factors.