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ANALISIS MOLEKULER FILOGENETIK DAN STRUKTUR ANTIGENIC VIRUS AVIAN INFLUENZA SUBTIPE H5N1 ISOLAT LAMPUNG TAHUN 2008-2013 Srihanto, Eko Agus; Asmara, Widya; Wibowo, Michael Haryadi
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2799

Abstract

Penelitian ini bertujuan melakukan karakterisasi molekuler antigenic site terhadap isolat virus avian influenza (AI) Balai Penyidikan dan Pengujian Veteriner (BPPV) Regional III Lampung dari tahun 2008-2013. Amplifikasi RNA dilakukan dengan teknik reverse transcription polymerase chain reaction (RT-PCR) menggunakan 4 pasang primer referens dari Australian Animal Health Laboratory (AAHL) Geelong Australia (HA10, HA20, dan HA30) dan dilanjutkan dengan proses pengurutan. Analisis hasil pengurutan dengan menggunakan perangkat lunak MEGA versi 5.05 yang meliputi multiple alignment, deductive amino acids prediction, dan phylogenic tree analysis diperoleh hasil perbedaan genetik antar isolat Lampung dari tahun 2003-2013 ditemukan berkisar 1,1-9,1% dengan tingkat homologi mencapai 90,9-98,9%. Variasi genetik ditemukan adanya substitusi pada posisi 53 (R53K), 126 (D126E), 136 (P136), 138 (H138Q, dan H138L), 140 (R140K, R140S, dan R140N), 141 (S141P), dan 189 (K189R). Berdasarkan analisis filogenic tree isolat Lampung tahun 2008-2011 termasuk ke dalam clade 2.1.3. Analisis filogenik isolat AI tahun 2012-2013 yang menginfeksi unggas air mempunyai homologi sekitar 98,5-99,1% dibandingkan dengan isolat AI yang menginfeksi unggas air asal Jawa dan termasuk ke dalam clade 2.3.2.1.
The establishment of PCR amplification, cloning, and sequencing of bovine herpesvirus 1 (BHV-1) glycoprotein D gene isolated in Indonesia Hidayati, Dewi Noor; Srihanto, Eko Agus; Untari, Tri; Wibowo, Michael Haryadi; Akiyama, Koichi; Asmara, Widya
Indonesian Journal of Biotechnology Article in Press
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.17 KB) | DOI: 10.22146/ijbiotech.44298

Abstract

Considering the increasing incidence of infectious bovine rhinotracheitis (IBR) in Indonesia, it is necessary to conduct a more in-depth study of bovine herpesvirus-1 (BHV-1) as the causative agent of IBR disease. Previous research reports indicate that one of the BHV-1 subtypes found in Indonesia is the subtype 1.1. Currently, IBR field case detection in Indonesia still uses the serological method (ELISA) that has the potential to give false positive results and cannot explain the virus subtype. Other detection methods such as viral isolation methods take longer time and require adequate resources. This study aimed to determine the BHV-1 subtypes of Indonesian isolates using molecular techniques. The nested PCR using two pairs of primers has succeeded. The nested PCR using two pairs of primers has succeeded to amplify the glycoprotein D (gD) gene. The gD gene fragment was cloned into the pGEM-T plasmid. The analysis of the gD gene sequence was carried out to determine the BHV-1 character of the Indonesian isolates. The results indicated that the isolates were different from the previous isolates, and had similarities (100%) with subtype 1.2 strain SP1777 and SM023.
AEVI-10 INVESTIGASI OUTBREAK MCF DI KABUPATEN MUSI RAWAS TAHUN 2016 Srihanto, Eko Agus; Siswanto, A J; Triwibowo, B
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.536 KB)

Abstract

Malignant Catarrhal Fever (MCF) merupakan penyakit viral yang menyerang sapi, rusa, bison, kerbau dan ruminansia lainnya. Sapi muda biasanya lebih rentan terutama yang dipelihara bersama domba (Damayanti, 2005). Penyakit ini kebanyakan berakibat dengan kematian (Teankam et al., 2000; Schultheiss, et al., 2000; Barker et al., 1993). MCF disebabkan oleh alcelaphine herpesvirus 1 (AlHV-1) dan ovine herpesvirus 2 (OvHV-2) (Fenner et al., 2011). Penyakit bersifat sporadik dengan tingkat  kematian dapat mencapai  100%  (Hamilton,  1990),  meskipun ada hewan yang sembuh setelah terserang MCF (Penny, 1998). Penyakit  MCF di  Indonesia dilaporkan pertama  kali  oleh  Paszotta  pada  tahun  1894  di Kediri,  Jawa  Timur  (Mansjoer,  (1954)  dalam Khatimah, dkk. 2014).  Penyakit  MCF telah  tersebar  hampir  di seluruh  Indonesia.  Namun di beberapa  daerah  banyak  kejadian  MCF  tidak terdiagnosis  atau  tidak dilaporkan.  Kerugian yang  ditimbulkan  akibat  penyakit  MCF  cukup besar  terutama  jika  kasus  penyakit  ini  terjadi pada hewan bibit. Kejadian penyakit MCF pada sapi bali di lapangan  sering  dikaitkan  dengan adanya ternak domba (Partadiredja et al., 1988). Lesi sapi penderita MCF dapat dilihat dari  gambaran  patologi anatomi dan histopatologi yang patognomonik  yaitu  vaskulitis  berupa  infiltrasi limfosit, makrofag, neutrofil  dan  sel  plasma pada  beberapa  organ seperti otak, paru-paru, hati, jantung dan usus (Liggit dan De Martini, 1980).  Kegiatan penyidikan kematian sapi bali  yang diduga disebabkan oleh Malignant Catarrhal Fever virus oleh tim Balai Veteriner Lampung di Kabupaten Musi Rawas dilaksanakan berdasarkan permohonan investigasi oleh Dinas Peternakan Kabupaten  Musi Rawas  mengenai adanya laporan kasus kematian sapi bali dengan gejala klinis mengarah pada penyakit  MCF di desa L Sidoharjo Kecamatan Tugu mulyo. Berdasarkan laporan tersebut maka Balai Veteriner mengeluarkan Surat Perintah Tugas No. 16001/TU.040/F5.C/09.2016 untuk melakukan penyidikan bersama dinas Peternakan Kabupaten Musi Rawas.Tujuan kegiatan adalah melakukan penyidikan kejadian kematian sapi bali di Kabupaten Musi Rawas, melakukan pengumpulan data epidemiologis, pengambilan spesimen di lapangan untuk mengetahui penyebab kematian sapi bali di Kabupaten Musi Rawas.
DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card L. Rustiati, Elly; Priyambodo, Priyambodo; Asiyah, Siti; Candra, Dedi; E. Anggraini, Diah; D. Krismuniarti, Elizabeth; Srihanto, Eko Agus; Angeliya, Liza; Nurcahyani, Nuning; Saswiyanti, Enny
The International Journal of Tropical Veterinary and Biomedical Research Vol 3, No 1 (2018): Vol. 3 (1) May 2018
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (189.698 KB) | DOI: 10.21157/ijtvbr.v3i1.11368

Abstract

Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephantmitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensivestrategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, geneticstudies have opened new field studies in ecology, included conservation ecology. Patterns in variation of populationhas been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’sID Card is a necessary in database building, which included morphology, health status, and genetic profile. Geneticprofile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNAisolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captivesumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oCand isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplificationanalysis. DNA isolation was successfully done, for further individual genetic ID building.
THE ESSENTIAL CONTRIBUTION OF CAPTIVE SUMATRAN ELEPHANT IN ELEPHANT TRAINING CENTER, WAY KAMBAS NATIONAL PARK FOR WILDLIFE GENETICS CONSERVATION Rustiati, Elly Lestari; Priyambodo, Priyambodo; Yulianti, Yanti; Srihanto, Eko Agus; Pratiwi, Dian Neli; Virnarenata, Elsa; Novianasari, Tika; Krismuniarti, Elisabeth Devi; Saswiyanti, Enny
BIOVALENTIA: Biological Research Journal Vol 6, No 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24233/BIOV.6.1.2020.173

Abstract

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants.  It shares its border with 22 of 37 villages surrounding the national park.  Understanding their existence in the wild is a priority, and  wildlife genetics is a crucially needed. Besides poaching and habitat fragmentation, wildlife-human conflict is one big issue.  Elephant Training Center (ETC) in WKNP is built for semi in-situ conservation effort on captive sumatran elephants that mainly have conflict histories with local people.  Participative observation and bio-molecular analysis were conducted to learn the importance of captive Sumatran elephant for conservation effort.  Through captive sumatran elephants, database and applicable methods are expected to be developed supporting the conservation of their population in the wild.  Participative observation and molecular identification was carried on captive sumatran elephants in ETC, WKNP under multiple year Terapan grant of Ministry of Research and Technology Higher Education, Indonesia. Gene sequence and cytological analyses showed that the captive sumatran elephants are closely related and tend to be domesticated.  Translocation among ETC to avoid inbreeding, and maintaining the captive sumatran elephant as natural as possible are highly recommended. Developing genetic database can be a reference for both captive and wild sumatran elephants.