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MICE OOCYTES RESPOND AFTER VITRIFICATION FOLLOWED BY ARTIFICAL ACTIVATION USING A VARIOUS CONCENTRATION OF STRONTIUM CHLORIDE AND CYTOCHALASIN B Ridlo, Muhammad Rosyid; Widyastuti, Rini; Lubis, Alkaustariyah; Fahrudin, Mokhamad; Boediono, Arief
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Oocyte vitrification is the important part of gamete preservation for further purpose. The objective of this study was to evaluate the response or development of vitrified-mice oocyte following activation using various concentrations of Strontium Chloride (Sr Cl2). Oocytes were collected from superovulation-induced female mice. Oocytes vitrification was then performed using a gradual equilibration of 2 M Ethylene Glycol in 0.25 M sucrose and 7 M Ethylene Glycol on 0.5 sucrose. Subsequently, the vitrified oocytes were thawed and activated using various Strontium Chloride concentration in each group. Control 1 is unvitrified oocyte and without Sr Cl2. Control 2 is unvitrified oocyte then activated by 20 mM Sr Cl2. Zero (0) mM  Sr Cl2 is vitrified oocyte without Sr Cl2. Group Ten (10) mM is vitrified oocyte then activated by 10 mM Sr Cl2. Group Twenty (20) mM is vitrified oocyte then activated by 20 mM Sr Cl2. The viability of vitrified-thawed oocytes was observed based on ooplasm integrity. Whereas the oocytes respond to artificial activation was observed based on pronucleus formation after 10 hours of activation. The result showed that 39% of oocyte degenerated following vitrification. The respond of vitrified-thawed oocytes following artificial activation using Strontium Chloride was significantly lower compared to fresh oocytes (p<0,05). Interestingly the highest percentage of activated oocytes (36.36%) was present in a group achieved 20 mM Strontium Chloride. As conclusion is Strontium Chloride 20mM has a best result (36,36%) to activate vitrified oocyte than 0 mM and 10 mM of Strontium Chloride. 
Penguatan Kelompok Tani Ternak Kerbau dan Introduksi Teknologi Reproduksi untuk Peningkatan Produktivitas Kerbau Lumpur di Kelompok Tani Ternak Kerbau Warnasari Kecamatan Plered Kabupaten Cirebon Widyastuti, Rini; Indika, Deru; Syamsunarno, Mas Rizky Anggun Adipurna; Budinuryanto, Dwi Cipto
Dharmakarya Vol 7, No 3 (2018): September
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.224 KB) | DOI: 10.24198/dharmakarya.v7i3.16583

Abstract

Populasi kerbau di kelompok Tani Ternak Kerbau Warnasari Kecamatan Plered Kabupaten Cirebon sekitar 328 ekor.  Walaupun telah terbentuk kelompok tani di wilayah tersebut belum dapat berfungsi secara optimal karena belum terbentuk struktur organisasi serta masih minimnya dinamika di dalam kelompok tersebut. Permasalahan lain yang dihadapi kelompok tersebut adalah terus menurunnya populasi kerbau disebabkan oleh rendahnya tingkat reproduksi kerbau, keterbatasan memanajemen ternak kerbau dan minimnya pengetahuan tentang aplikasi teknologi reproduksi. Kegiatan Pengabdian Masyarakat ini dilaksanakan untuk penguatan kelompok budidaya kerbau, pengaktifan dinamika pada kelompok peternak kerbau, dan pengenalan teknologi reproduksi. Metode pengabdian yang dilakukan adalah melakukan pelatihan organisasi dan pengenalan teknologi reproduksi kepada kelompok ternak kerbau Warnasari. Kegiatan yang telah dilakukan penyuluhan dengan materi meliputi:  penguatan organisasi kelompok tani dan. pengenalan teknologi reproduksi dalam beternak kerbau.  Hasil yang dipeoleh adalah terbentuknya struktur organisasi dan meningkatnya pemahaman masyarakat dalam penerapan manajemen beternak kerbau seta aplikasi teknologi reproduksi untuk meningkatkan produktivitas kerbau.
Tingkat Pengetahuan dan Respon Peternak Kambing Perah terhadap Penyakit Hewan Studi Kasus: Kelompok Tani “Simpay Tampomas” Cimalaka, Sumedang Widyastuti, Rini; Wira, Dwi Wahyudha; Ghozali, Mohammad; Winangun, Kikin; Syamsunarno, Mas Rizky Anggun Adipurna
Dharmakarya Vol 6, No 2 (2017): Juni
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.952 KB) | DOI: 10.24198/dharmakarya.v6i2.14812

Abstract

Peternakan kambing perah merupakan mata pencaharian utama masyarakat Kecapatan Cimalaka, Kabupaten Sumedang. Pengelolaan peternakan masih dilakukan dengan cara tradisiona, sehingga perlu dilakukan upaya peningkatkan pengetahuan kelompok peternak kambing perah mengenai pengelolaan manajemen kesehatan kambing perah serta mencegah terjadinya kerugian akibat dampak penyakit.  Salah satunya adalah melalui kegiatan penyuluhan mengenai penyakit-penyakit pada ternak terutama dari aspek klinis. Kegiatan diawali dengan survei lokasi, pemberian vitamin pada kambing perah, penyuluhan, pengisian kuesioner, pengolahan hasil kuesioner. Pada tahap akhir, dilakukan timbal balik (feedback) pada peternak atas hasil yang didapatkan dari pengobatan dan kuesioner. Hasil menunjukkan bahwa tangka pengetahuan peternak terhadap penyakit hewan dan cara pencegahannya sudah cukup baik. Kasus yang banyak berkembang di daerahpeternakan tersebut adalh Scabies, mastitis dan Bloat dengan gejala umum berkurangnya nafsu makan dan demam. Peternak biasanya memberikan pertolongan pertama dengan memberikan air asam dan obat cacing. Berdasarkan hasil tersebut, dapat bahwa peternak telah memiliki tingkatpengetahuan penyakit yang baik tetapi belum memiliki pengetahuan untuk penangann penyakit secara memadai. 
Tingkat Pengetahuan Peternak Kambing Perah Terhadap Penyakit Zoonosis Studi kasus “Kelompok Tani ternak Sympay Tampomas” Cimalaka Hartady, Tyagita; Widyastuti, Rini; Ghozali, Mohammad
Dharmakarya Vol 7, No 3 (2018): September
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.182 KB) | DOI: 10.24198/dharmakarya.v7i3.18401

Abstract

Hubungan antara peternak, hewan ternak, dan lingkungan sangat signifikan sehingga diperlukan studi untuk dapat mengetahui hubungan ketiga komponen tersebut terhadap penyakit zoonosis. Kegiatan diawali dengan menilai keadaan umum lingkungan peternakan dan kondisi kambing perah melalui tanya jawab berbagai faktor risiko timbulnya penyakit zoonosis, penyuluhan kepada peternak mengenai penyakit-penyakit yang umum menyerang kambing perah dan pentingnya pengelolaan kesehatan kambing perah. Sebanyak 75% peternak telah membuat kandang kambing dengan model panggung, beralas bambu, serta memiliki kisi-kisi di dindingnya untuk memudahkan perputaran udara, 25% kandang lainnya dibuat dengan model terbuka dan kambing langsung kontak dengan lantai. Jarak antar kandang di daerah ini umumnya tidak saling berdekatan. Di sisi lain, sejumlah 16,7 % peternak merupakan kelompok peternak berumur lebih dari 47 tahun dan beresiko tinggi terhadap infeksi penyakit. Sedangkan 16,7% peternak mengerti tentang penyakit zoonosis dari kambing ke manusia didukung dengan rendahnya upaya peternak dalam melakukan tindakan pencegahan terhadap infeksi zoonosis. Sebanyak 83,3% peternak cenderung tidak melakukan pencegahan terjadinya penyakit zoonosis. Mengenai perawatan kesehatan kambing, rata-rata 48% peternak tidak melakukan tindakan-tindakan pencegahan penyakit infeksi pada kambingnya. Kondisi ini berpotensi menyebabkan  kambing terjangkit penyakit dan tidak menutup kemungkinan menular ke ternak lainnya atau ke peternak.
Penyuluhan kesehatan reproduksi sapi perah pada peternak sapi perah di Cipageran, Cimahi, Jawa Barat Hartady, Tyagita; Widyastuti, Rini
ARSHI Veterinary Letters Vol 3, No 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (269.44 KB) | DOI: 10.29244/avl.3.1.17-18

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Cipageran, Cimahi city, West Java has a high population of dairy cattles and one of the suppliers of milk for the West Java region. However, there are limitations in farmers' knowledge about animal health, especially reproductive disorder. Data collection was carried out in the local dairy cattle farmer group. The method used were a pre-test on the reproductive health of dairy cattles, counseling on reproductive health and prevention of reproductive disorders, discussion related to the topic provided. Based on the results of the question and answer and discussion, information on the level of knowledge of the breeders' groups on the reproductive health of dairy cattles was obtained and efforts to prevent the emergence of reproductive disorders were good enough. However, the knowledge of farmers in order to prevent, handle and care for livestock is still lacking, so better support are needed.
The influence of experience and confidence on the health man-agement of dairy goat (Case study: “Simpay Tampomas Farmer Group” Village Cilengkrang, Cimalaka District of Sumedang, West Java-Indonesia) Hartady, Tyagita; Widyastuti, Rini
ARSHI Veterinary Letters Vol 2, No 3 (2018): ARSHI Veterinary Letters - Agustus 2018
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.807 KB) | DOI: 10.29244/avl.2.3.49-50

Abstract

Dairy goat is profitable object since the productivity is relatively brief, easy in maintenance and does not require much investment. Dairy goat become one of the important commodity for farmers in the village Cilengkrang, Cimalaka District of Sumedang. This study used a qualitative approach where 18 farmers who become interviewees. Collected data were observed and compared with the evaluation on the participants’ routine. The majority farmers are depending on individual experiences and conclusions without related guidance and supported references of diseases and procedures in nurturing dairy goats (61.1%), while the rest are consulting the problem to the vet or the group (83,3%). Some of them used the conventional treatment. However, when the condition of sick animal never improved, only 83,3% of farmers who contact veterinarian and the rest would prefer sell the animal in the market. It can be concluded that the majority farmers in the village, has experienced to overcome certain diseases of dairy goats. Nevertheless, the knowledge and sources of supporting references are still limited that affect to the use of inappropriate traditional herbs or regular human medicines from nearby stalls is still relatively high, so the awareness and knowledge of breeders about health management of dairy goat of breeders needs to be improved.
LOW CONCENTRATION OF ETHYLENE GLYCOL IMPROVED RECOVERY RATE OF HUMAN SPERMATOZOA AFTER VITRIFICATION (ETILEN GLIKOL KONSENTRASI RENDAH MENINGKATKAN RECOVERY RATE SPERMATOZOA MANUSIA PASCAVITRIFIKASI) Widyastuti, Rini; Sumarsono, Sony Heru; Boediono, Arief; Rasad, Siti Darodjah
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of cryoprotectants for the cryopreservation of human spermatozoa, oocytes, zygote, earlycleavage stage of embryos and blastocyst is an integral part of almost every human In Vitro Fertilizationprogram. Moreover, the cryopreservation of these types of cells by direct plunging into liquid nitrogen (-196°C) usually requires a high concentration of cryoprotectant with a consequent of cytotoxic effect. Theaim of this study was to observe the effect of ethylene glycol concentration on the spermatozoa recoveryrate following vitrification process. Earle?s balanced salt solution + 0.25 M sukrosa + 1 % human albumineserum as basic solution supplemented with some different concentrations of etylene glycol (ie: 36.25%;18.25%; 9.12%; 4.56%; 1.14% and 0.57%) were used to evaluate the motility and viability of spermatozoafollowing vitrification. Human?s spermatozoa from ejaculates with progressive motility and viability above50% were used as samples. Samples were mixed with vitrification solution and then loaded into 0.25 mLstraws, equilibrated for 10 minutes at room temperature before plunged into liquid nitrogen. Spermatozoathawing was done at 24 hours after the vitrification. The results showed that, the decrease of spermatozoamotility and viability were observed at the highest (100%, 96.70%, respectively) in the samples that wereadded with vitrification medium contained 36.25% of ethylene glycol. On the other hand, the decrease ofthe spermatozoa motility and viability were found at the lowest (14.11%, 43.81 %, respectively) in thesamples without ethylene glycol supplementation. It can be concluded that the highest spermatozoa recoveryrate was obtained from the vitrification using a low concentration of ethylene glycol.
PENGARUH KEBERADAAN CORPUS LUTEUM TERHADAP KUALITAS DAN TINGKAT MATURASI OOSIT DOMBA LOKAL UMUR PUBERTAS AWAL SECARA IN VITRO Widyastuti, Rini; Syamsunarno, Mas Rizky Anggun Adipurna; Yusuf, Alvin; Ridhlo, Muhammad Rosyid; Prastowo, Sigit
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.503 KB) | DOI: 10.17969/agripet.v18i2.12103

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ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium domba umur pubertas awal terhadap kualitas oosit hasil koleksi dan tingkat maturasinya secara in vitro (IVM). Sebanyak 279 oosit digunakan, terbagi pada kelompok tanpa CL (CL-) sebanyak 143 dan dengan CL (CL+) sebanyak 136. Oosit dipilih berdasarkan homogenitas sitoplasma dan dikelompokkan sesuai jumlah lapisan sel kumulus yaitu grade 1 ( 4 lapis), grade 2 (3?4 lapis) dan grade 3 (0?2 lapis). Media IVM menggunakan Tissue Culture Media 199 ditambah antibiotik, Follicle Stimulating Hormone, dan 10% Fetal Bovine Serum. Maturasi dilakukan pada inkubator 38,5°C, 5% CO2 selama 24 jam. Pasca IVM, tingkat kematangan oosit dievaluasi berdasar kemunculan Polar Body I (PB I). Hasil menunjukkan bahwa keberadaan CL tidak berpengaruh terhadap kualitas oosit yang dikoleksi pada semua grade. Keberadaan CL berpengaruh pada tingkat kematangan oosit pada grade 1 sebesar 48,64% dibandingkan CL- sebesar 47,19% (p 0,05). Berdasarkan hasil penelitian disimpulkan bahwa berpengaruh pada tingkat kematangan oosit setelah IVM. Hasil penelitian menggambarkan potensi penggunaan oosit ternak umur pubertas awal untuk digunakan lebih lanjut dalam program produksi embrio secara in vitro. (Effect of the presence of corpus luteum on oocytes quality and in vitro maturation rate of ewes at early puberty) ABSTRACT. This study aims to know effect of presence of corpus luteum (CL) to collected oocytes quality and its maturation rate post in vitro maturation (IVM), on local ewes ovary at early puberty. In total 279 oocytes were collected, 143 without CL (CL-) and 136 with CL present (CL+). Oocytes were selected according to sitoplasma hemogenity, divided into 3 grades according to cumulus cell (CC) layer namely Grade 1, 2 and 3 indicated by 4, 3?4, and 0?2 CC layers, respectively. The IVM media was Tissue Culture Media 199 supplemented with antibiotic, Follicel Stimulating Hormone, and 10% Fetal Bovine Serum following culture at 38.5°C and 5% CO2. Twenty four hours post IVM, oocytes were evaluated on the presence of Polar Body I. Result showed that oocytes quality were not different among group in all grades. The present of CL gives better maturation rate in grade 1 compared to CL- (48.64 vs 47.19%; p 0.05). The present finding show that the presence of CL improves oocytes maturation rate post IVM. Moreover, this study shows the potency of using oocytes from ewes ovary at early puberty for further in vitro embryo production program
IMPROVING EWE OOCYTE VIABILITY AFTER VITRIFICATION WARMING USING COMBINATION OF DIFFERENT CRYOPROTECTANT AND CARRIER SYSTEM Widyastuti, Rini; Setiawan, Rangga; Syamsunarno, Mas Rizky Anggun Adipurna; Ghozali, Mohammad; Saili, Takdir; Boediono, Arief
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.347 KB) | DOI: 10.21157/j.ked.hewan.v12i3.11398

Abstract

The aim of this study was to determine the best combination of cryoprotectant (Ethylene glycol, EG), dimethyl sulfoxide (DMSO) and propanediol (PrOH) and carrier system (hemistraw and cryotop) in improving ewe oocytes viability during cryopreservation. Oocytes with multi layers of compact cumulus cells were colleted from abbatoir and matured in TCM 199 medium supplemented with 10% fetal calf serum for 24-26 h at 38.5° C under 5% CO2 in the air. Matured oocyte was divided into six parts and vitrified in three different vitrification solutions; (i) 17% EG+17% DMSO with hemistraw as carrier system, (ii) 34% EG with hemistraw as carrier system, (iii) 17% EG+17% PrOH in hemistraw (iv), 17% EG+17% DMSO with cryotop as carrier system (v), 34% EG with cryotop as carrier system (vi), and 17% EG+17% PrOH in cryotop. Oocytes were cryopreserved for one week before revived and evaluated for viability. The result showed that oocytes vitrified in media containing EG and DMSO in cryotop had the highest viability (88.16%) compared to media containing EG only or EG and PrOH (70.95% and 68.76%, respectively) (P 0.05). Moreover, oocytes viability that vitrified using cryotop and hemistraw as carrier system were not significantly different. The present results indicated that vitrification using combination of EG and DMSO as permeable cryoprotectant and cryotop as carrier system was the best system to maintain oocyte viability after vitrification-warming.
EFFECT OF PMSG ADMINISTRATION IN COMBINATION WITH VAGINAL SPONGE ON ESTROUS OCCURRENCE AND LITTER SIZE OF JAVANESE SHEEP Setiawan, Rangga; Rasad, Siti Darodjah; Solihati, Nurcholidah; Widyastuti, Rini; Soeparna, Soeparna
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.21 KB) | DOI: 10.21157/j.ked.hewan.v11i4.4349

Abstract

AbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100%  of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively.  Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, sheep