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PEMANFAATAN LIMBAH CAIR SAGU UNTUK MEMPRODUKSI SELULOSA BAKTERI Ahmad, Sitti Wirdhana; Yanti, Nur Arfa; Muhiddin, Nurhayani H.
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3763

Abstract

ABSTRACTBacterial cellulose is an exopolysaccharide produced by bacteria and has a high purity level compared to plant cellulose and has unique structural and mechanical characteristics that can be utilized for various industrial purposes such as food, medical, plastic and paper. This study aims to determine the potential of sago liquid waste as a substrate for producing biocellulose and sugar concentration is required in producing bacterial cellulose from sago liquid waste. Production of bacterial cellulose from sago liquid waste was done with static condition for 14 days with treatment of sugar concentration 5,10,15 and 20% (w/v) using Acetobacter xylinum. Parameters were measured include of thickness, yield, crude fiber content and moisture content. Production of bacterial cellulose using sago liquid waste requires the addition of sugar as much as 10% with a thickness of 21.73 mm, yield of 34.97%, crude fiber of 4.5% and moisture content of 91.35%. Therefore, sago liquid waste is potentially used as a substrate for producing bacterial cellulose.  Keywords :Biocellulose, nata, Acetobacter xylinum, Production substrate  
ANALISIS KEMAMPUAN MENULIS MODUL PEMBELAJARAN IPA TERPADU MAHASISWA CALON GURU IPA Rusli, Muhammad Aqil; Muhiddin, Nurhayani H.
Indonesian Journal of Educational Studies Vol 22, No 2 (2019): Indonesian Journal of Educational Studies
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.396 KB) | DOI: 10.26858/ijes.v22i2.11777

Abstract

Abstrak. Penelitian ini bertujuan untuk mengetahui kemampuan menulis modul pembelajaran IPA terpadu mahasiswa calon guru IPA Program Studi Pendidikan IPA FMIPA UNM. Kemampuan tersebut dilihat dari hasil validasi dua orang ahli untuk menilai aspek format, isi, dan bahasa dalam penyusunan modul. Selain itu, akan dijelaskan aspek keterpaduan yang termuat dalam analisis tema penyusunan modul serta kesesuaian antara keterpaduan dengan model fragmented, connected dan nested. Subjek dalam penelitian ini adalah modul IPA terpadu berbasis keterampilan proses sains pada materi pokok cahaya dan alat optik yang telah dikembangkan oleh mahasiswa calon guru IPA Program Studi Pendidikan IPA. Berdasarkan penilaian dari dua orang pakar maka dapat disimpulkan bahwa modul tersebut layak digunakan sebagai modul pembelajaran IPA terpadu. Ditinjau dari kerangka analisis materi keterpaduan, terlihat bahwa seluruh materi telah didesain secara terpadu, meskipun tidak semua materi tersebut memiliki keterkaitan secara langsung dengan topik yang ditampilkan. Untuk keterkaitan dengan keterampilan proses sains, maka secara eksplisit telah memperlihatkan keterkaitan antara aspek pengetahuan dengan keterampilan proses sains, namun keterampilan sosial dan keterampilan mengorganisasikan data belum dituliskan secara eksplisit. Padahal model keterpaduan nested secara eksplisit mendeskripsikan setiap aspek keterampilan yang akan dipadukan. 
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian; Muhiddin, Nurhayani H.
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.882 KB)

Abstract

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB.Keywords : Production, PHB, Amylolytic bacteria, Sago starch.
A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria Yanti, Nur Arfa; Sembiring, Langkah; Margino, Sebastian; Muhiddin, Nurhayani H.
Indonesian Journal of Biotechnology Vol 18, No 2 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.882 KB) | DOI: 10.22146/ijbiotech.7877

Abstract

Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl ether method and PHB contentwas quantifi ed with UV spectrophotometer at 235 nm. Glucose level was determined by using kit of glucoseGOD 10” and was quantifi ed with spectrophotometer at 500 nm. Sago starch concentration was determinedby phenol method using specthrophotometer at 490 nm. The result of the study showed that Bacillus sp.PSA10 was produced PHB up to 66,81 % (g PHB/g cell dry weight) at 48 h and Bacillus sp. PPK5 up to 24,83% (g PHB/g cell dry weight) at 84 h. Bacillus sp. PSA10 has ability to converse sago starch to be PHB directlywithout glucose accumulation in the media, whereas Bacillus sp. PPK5 have to accumulate glucose as productof sago starch hydrolysis to produce of PHB. PHB synthesis by Bacillus sp. PHB production on sago starchof the Bacillus sp. PSA10 was found to be growth-associated whereas Bacillus sp. PPK5 was found to be nongrowth-associated. Therefore, two indigenous amylolytic bacteria were having of difference in biosynthesismechanism of PHB in sago starch medium and their characteristics of PHB synthesis should be consideredin developing cultivation methods for the effi cient production of PHB. Keywords : Production, PHB, Amylolytic bacteria, Sago starch.
Isolasi dan Seleksi Bakteri Pendegradasi Solar Dari Pelabuhan Penyeberangan Kendari – Wawonii, Sulawesi Tenggara Mijaya, M. Rajab Sutra; Yanti, Nur Arfa; Ardiansyah, Ardiansyah; Muhiddin, Nurhayani H.
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.347 KB) | DOI: 10.33772/biowallacea.v6i2.8825

Abstract

This study aimed to obtain the ability of bacteria to degrade diesel fuel. Method of this research was exploration method. Bacteria were isolated by enrichment method used SMSSe enriched diesel 2% (v/v). Selection of hidrocarbonoclastic bacteria based on the ability of to grow on agar medium solid. The bacteria in the test made in the form of suspension with Mc Farland Standard 0.5. Test of bacterial isolates were used 10% of the inoculum put in 150 mL media with different concentrations of diesel fuel were 1%, 2% and 3% and incubated on a rotary shaker at 120 rpm. Samples were taken on 1, 5, 10, 15 and 20 days to test diesel emulsion by centrifugation at a speed of 3500 rpm ±15 minutes, the comparison between the media and diesel 4:1. Growth in the amount of bacteria accounted by a Standard Plate Count method. The levels of the diesel rest calculated every sampling during incubation. The selection results of obtained diesel degrading bacteria isolates that PSI.1 PSII.1 and PSIII.2. All of bacteria have the ability to degrade diesel fuel in different treatment, the best result in lowered diesel fuel that were a concentration of 3% during 20 days of incubated at PSII.1 isolate, that have the highest ability to reduce levels of diesel up to 70,70%.Keywords: Hydrocarbonoclastic, Degradation, Diesel fuels ABSTRAK Penelitian ini bertujuan untuk mengetahui kemampuan bakteri dalam mendegradasi solar. Penelitian ini adalah penelitian eksploratif. Isolasi bakteri menggunakan metode enrichment dengan media yang digunakan yaitu SMSSe yang diperkaya solar 2% (v/v). Pemilihan bakteri hidrokarbonoklastik berdasarkan kemampuan bakteri tumbuh pada media agar padat. Inokulum bakteri dibuat dalam bentuk suspensi dengan Standar Mc Farland 0,5. Pengujian kemampuan degradasi solar dilakukan menggunakan media minimal dengan variasi konsentrasi solar 1%, 2% dan 3% dan diinkubasi pada rotary shaker. Sampel diambil pada hari ke 1, 5, 10, 15 dan 20 untuk uji emulsi solar dengan menghitung volume solar yang teremulsi. Pertumbuhan jumlah bakteri dihitung dengan metode Standard Plate Count. Kadar sisa solar dihitung setiap pengambilan sampel selama inkubasi. Hasil seleksi bakteri pendegradasi solar diperoleh tiga isolat yaitu PSI.1 PSII.1 dan PSIII.2. Ketiga isolat bakteri memiliki kemampuan mendegradasi solar pada perlakuan yang berbeda, hasil terbaik dalam menurunkan kadar solar yaitu konsentrasi 3% selama 20 hari inkubasi pada isolat PSII.1 yang memiliki kemampuan tertinggi menurunkan kadar solar hingga 70,70%. Kata kunci : Hidrokarbonoklastik, Pendegradasi, Solar
Analisis Kuantitatif Mikroorganisme pada Ragi Tape Lokal dan Daya Terima Tape Jusinta yang dihasilkan Muhiddin, Nurhayani H.; Ramlawati, Ramlawati; Yanti, Nur Arfa; Mun'im, Abdul
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 6, No 2 (2019): BioWallacea and Biotechnological Science
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.347 KB) | DOI: 10.33772/biowallacea.v6i2.8950

Abstract

The purpose of this study were to analyze the quantity of microorganisms in some local ragi tape and determine the panelists' acceptance of the "tape jusinta" was produced. This type of research is an experiment with a solid substrate fermentation method. The used ragi tape is 4 kinds of local ragi tape from South Sulawesi Province (ragi tape A, B, C, and D) and 1 type of local ragi tape from Southeast Sulawesi Province (ragi tape E). The Tape Jusinta was produced by fermentation of a solid mixture of purple sweet potato and cassava tubers in a ratio of 1: 1 using 5 kinds of local ragi tape. Analysis of microbial quantity in ragi tape includes the number of molds, yeasts and bacteria in viable count using the Standard Plate Count (SPC) method. Panelist acceptance of the Tape Jusinta product was analyzed using the Hedonic test with a numerical scale range of "6". Data were analyzed descriptively and inferentially using Anova and Duncan's Multiple Test. The results of the data analysis showed that the level of palatability of the panelists to the tapejusinta  fermentedragi tape B was not different from the tape jusinta fermented ragi tape A and C, but the tape jusinta fermented by Cragi tape was different from the results of fermentation ragi tape D and E. The ?tape jusinta? fermented ragi tape B is preferred over the tape jusinta fermented ragi tape A, C, D and E with the highest average panelist rating of 4.15. The quantity of microorganisms on ragi tape B are mold types as much as 1.0 x 104 cfu / g, yeast 8.0 x 103 cfu / g and bacteria 5.7 x 104 cfu/ g. Keywords: cassava roots, purple sweet potatoes, ragi tape, "tape jusinta", palatabilityAbstrakTujuan penelitian adalah menganalisis kuantitas mikroorganisme pada beberapa ragi tape lokal dan mengetahui daya terima panelis terhadap ?tape jusinta? yang dihasilkan. Jenis penelitian adalah eksperimen dengan metode fermentasi substrat padat. Ragi tape yang digunakan adalah 4 macam ragi tape lokal yang berasal dari Provinsi Sulawesi Selatan (ragi tape A, B, C, dan D) dan 1 macam berasal dari Provinsi Sulawesi Tenggara (ragi tape E). Tape Jusinta dihasilkan melalui fermentasi substrat padat campuran ubi jalar ungu dan umbi ubi kayu dengan perbandingan 1 : 1 menggunakan 5 macam ragi tape lokal. Analisis kuantitas mikroba pada ragi tape  meliputi jumlah kapang, khamir dan bakteri secara viable countmenggunakan metode Standard Plate Count (SPC). Daya terima panelis terhadap produk Tape Jusinta dianalisis menggunakan uji Hedonik dengan rentang skala numerik ?6?.Data dianalisis secara deskriptif dan inferensial dengan Anava dan Duncan?s Multiple Test. Hasil analisis data menunjukkan bahwa tingkat kesukaan (palatability) panelis terhadap tape jusinta hasil fermentasi ragi tape B tidak berbeda dengan tape jusinta hasil fermentasi ragi tape A dan C, namun tape jusinta hasil fermentasi ragi tape C berbeda dengan hasil fermentasi ragi tape D dan E. Tape jusinta hasil fermentasi ragi tape B lebih disukai daripada tape jusinta hasil fermentasi ragi tape A, C, D dan E dengan rata-rata penilaian panelis tertinggi yaitu 4.15. Kuantitas mikroorganisme pada ragi tape B yaitu jenis kapang sebanyak 1.0 x 104 cfu/g, khamir   8.0 x 103 cfu/g dan bakteri 5.7 x 104 cfu/g .Kata kunci:  Umbi ubi kayu, ubi jalar ungu, ragi tape, ?tape jusinta?, daya terima
PEMANFAATAN LIMBAH CAIR SAGU UNTUK MEMPRODUKSI SELULOSA BAKTERI Ahmad, Sitti Wirdhana; Yanti, Nur Arfa; Muhiddin, Nurhayani H.
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3763

Abstract

ABSTRACTBacterial cellulose is an exopolysaccharide produced by bacteria and has a high purity level compared to plant cellulose and has unique structural and mechanical characteristics that can be utilized for various industrial purposes such as food, medical, plastic and paper. This study aims to determine the potential of sago liquid waste as a substrate for producing biocellulose and sugar concentration is required in producing bacterial cellulose from sago liquid waste. Production of bacterial cellulose from sago liquid waste was done with static condition for 14 days with treatment of sugar concentration 5,10,15 and 20% (w/v) using Acetobacter xylinum. Parameters were measured include of thickness, yield, crude fiber content and moisture content. Production of bacterial cellulose using sago liquid waste requires the addition of sugar as much as 10% with a thickness of 21.73 mm, yield of 34.97%, crude fiber of 4.5% and moisture content of 91.35%. Therefore, sago liquid waste is potentially used as a substrate for producing bacterial cellulose.  Keywords :Biocellulose, nata, Acetobacter xylinum, Production substrate  
PENGARUH MODEL PEMBELAJARAN DISCOVERY LEARNING TERHADAP HASIL BELAJAR PESERTA DIDIK KELAS IX SMP NEGERI 31 MAKASSAR PADA MATERI LISTRIK STATIS Taufiq, A. Husnawati; Muhiddin, Nurhayani H.; Yunus, Sitti Rahma
Jurnal IPA Terpadu Vol 2, No 1 (2018)
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/ipaterpadu.v2i1.11149

Abstract

Penelitian ini bertujuan untuk mengetahui ; (1) Tingkat hasil belajar peserta didik kelas IX SMP Negeri 31 Makassar pada materi pokok Listrik Statis dengan menggunakan model pembelajaran Discovery Learning. (2) Hasil belajar peserta didik kelas IX SMP Negeri 31 Makassar pada materi pokok Listrik Statis dengan menggunakan model pembelajran langsung. (3) Ada tidaknya pengaruh positif model pembelajaran Discovery Learning terhadap hasil belajar peserta didik SMP Negeri 31 Makassar pada materi pokok Listrik Statis. Instrumen untuk mendapatkan data hasil belajar berupa soal pilihan ganda sebanyak 20 nomor, diberikan di akhir penelitian. Untuk mengetahui ada tidaknya pengaruh model pembelajaran Discovery Learning terhadap hasil belajar, data yang diperoleh dilakukan analisis statistik deskriptif dan statistik inferensial untuk kedua kelas. Hasil analisis data deskriptif diperoleh untuk kelas eksperimen nilai rata-rata sebesar 82,41 dan kelas kontrol rata-rata sebesar 76,30 masuk pada kategori tinggi. Berdasarkan hasil uji hipotesis diperoleh bahwa Ho ditolak dan Ha diterima dimana nilai thitung = 2,93 > ttabel  = 1,3  yang artinya ada pengaruh positif model pembelajaran Discovery Learning terhadap hasil belajar peserta didik SMP Negeri 31 Makassar pada materi pokok Listrik Statis.
PENGEMBANGAN MEDIA PEMBELAJARAN BERBASIS GAME PENYELAMAT LINGKUNGAN (PENLING) Fakhirah, Nurul; Muhiddin, Nurhayani H.; Yunus, Sitti Rahma
Jurnal IPA Terpadu Vol 2, No 2 (2019)
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/ipaterpadu.v2i2.11168

Abstract

Penelitian ini bertujuan untuk : Mengatahui (1) Kevalidan media pembelajaran berbasis game penyelamat lingkungan (PENLING) yang telah dikembangkan untuk materi pencemaran lingkungan (2) Efektivitas media pembelajaran berbasis game penyelamat lingkungan (PENLING) pada materi pencemaran lingkungan (3) Kepraktisan penggunaan media pembelajaran berbasis game pada materi pencemaran lingkungan. Penelitian ini merupakan penelitian pengembangan (Research and Development). Subjek penelitian ini adalah peserta didik kelas VII SMPN 3 Makassar. Hasil penelitian menunjukkan bahwa kevalidan media pembelajaran berbasis game PENLING berada pada kategori valid. Keefektifan game PENLING yang diukur dari N-Gain peserta didik yaitu 0,6 kategori sedang. Kepraktisan game PENLING oleh respons pendidik menunjukkan nilai rata-rata 83,33 dan respons peserta didik 94,25 menunjukkan kriteria sangat positif . Berdasarkkan hasil penelitian maka dapat disimpulkan bahwa media pembelajaran berbasis game PENLING yang dikembangkan memenuhi kriteria kevalidan, keefektifan  dan kepraktisan
STUDI KETERAMPILAN PROSES SAINS (KPS) PESERTA DIDIK KELAS VIII SMP NEGERI 12 MAKASSAR Saleh, Salsabila Yusuf; Muhiddin, Nurhayani H.; Rusli, Muhammad Aqil
Jurnal IPA Terpadu Vol 3, No 2 (2020)
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/ipaterpadu.v3i2.11294

Abstract

Penelitian ini bertujuan untuk mengetahui tingkat keterampilan proses sains peserta didik kelas 8 SMP Negeri 12 Makassar. Penelitian ini merupakan penelitian deskriptif kuantitatif dengan menggunakan metode penelitian survey. Populasi penelitian ini adalah seluruh peserta didik kelas VIII SMP Negeri 12 Makassar sebanyak 379 peserta didik. Sampel pada penelitian ini berjumlah 80 peserta didik dari 11 kelas yang berbeda yang dipilih melalui teknik random sampling. Instrumen penelitian berupa tes keterampilan proses sains berupa soal pilihan ganda berjumlah 25 item soal yang mewakili setiap aspek keterampilan proses sains: mengobservasi, merumuskan masalah, membuat hipotesis, merancang percobaan, mengkomunikasikan, menarik kesimpulan. Teknik pengumpulan data pada penelitian ini adalah dengan memberikan tes keterampilan proses sains kepada sampel penelitian. Analisis data dilakukan dengan menggunakan analisis deskriptif dan inferensial. Analisis deskriptif digunakan untuk menyelidiki skor rata-rata keterampilan proses sains peserta didik dan skor rata-rata peserta didik adalah sebesar 15, 24 dan berada dalam kategori sedang. Sedangkan analisis inferensial digunakan untuk mengetahui taksiran rata-rata keseluruhan populasi, diperoleh taksiran skor rata-rata untuk keseluruhan populasi berada dalam rentang 14, 41 sampai dengan 16, 04 yang berarti berada dalam kategori sedang dan tinggi.