Rani Agustina Wulandari, Rani Agustina
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PENGEMBANGAN METODE PENYARINGAN KLON TEBU TAHAN KERING MENGGUNAKAN METODE PENGENDALIAN KADAR LENGAS Pinilih, Yasinta; Taryono, Taryono; Wulandari, Rani Agustina
Vegetalika Vol 8, No 4 (2019)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (248.263 KB) | DOI: 10.22146/veg.38433

Abstract

Tebu merupakan salah satu tanaman pengahasil gula yang digunakan di Indonesia. Luas areal tebu di lahan sawah beririgasi di Jawa semakin berkurang. Kini areal tebu di lahan sawah tinggal sekitar 40 %, selebihnya telah beralih ke lahan kering. Pada fase perkecambahan,  kebutuhan air tanaman rendah, kemudian mulai meningkat pada fase pertunasan dan mencapai puncaknya pada fase pemanjangan batang. Tujuan penelitian ini, akan dilihat ketahanan bibit tebu terhadap cekaman saat fase perkecambahan dan proses beradaptasinya kembali tebu saat fase pertumbuhan tunas dari kondisi tercekam. Penelitian dilakukan pada bulan Mei hingga September 2016 di Kebun Percobaan Banguntapan, Fakultas Pertanian, Universitas Gadjah Mada. Rancangan Percobaan yang digunakan yaitu Rancangan Acak Lengkap (RAL) dengan 2 faktor. Faktor pertama yaitu kadar lengas yang terdiri dari 4 aras yaitu 100%, 80%, 60%, dan 40%. Faktor kedua adalah klon tebu yaitu klon PS 862, PS 864, Kidang Kencana (KK), Bululawang (BL), dan VMC 76-16. Hasil menunjukkan bahwa perlakuan pengaturan kadar lengas mampu mempengaruhi pertumbuhan tebu dengan menurunnya angka pertumbuhan beberapa klon ketikakadar lengas diturunkan hingga 40%. Klon KK menjadi klon yang memiliki hasil terendah dan kurang tahan terhadap perlakuan cekaman kekeringan
PENGARUH PEMERAMAN EKSPLAN DAUN DENGAN KOLKISINA SECARA IN VITRO TERHADAP KEBERHASILAN PEMBENTUKAN TERUNG TETRAPLOID Nabihaty, Fathin; Taryono, Taryono; Wulandari, Rani Agustina
Vegetalika Vol 7, No 1 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (937.085 KB) | DOI: 10.22146/veg.33555

Abstract

Perbaikan tanaman untuk meningkatkan daya tawar terung dapat dilakukan melalui poliploidisasi. Pemberian kolkisina secara in vitro berpeluang meningkatkan efisiensi poliploidisasi. Penelitian dilakukan untuk mengkaji pengaruh pemeraman eksplan daun dengan kolkisina secara in vitro terhadap keberhasilan poliploidisasi dan pembentukan terung tetraploid (4x), serta mendapatkan tetraploid berdasarkan ciri morfologi planlet dan jumlah kromosom. Tiga aras periode peram dengan 2,5 mM kolkisina (24, 48, dan 72 jam) dan satu perlakuan pemeraman tanpa kolkisina sebagai pembanding, diujicobakan pada lima jenis terung (Rimbang, Lokal Bantul, Pipit, Hijau Malang, dan Limao) menggunakan rancangan acak lengkap. Tahap utama yang dilalui eksplan yaitu pra-pemeraman, pemeraman dengan kolkisina, regenerasi, penggantian media tanam 1-6 kali, pengakaran, dan regenerasi ulang hingga 2 kali. Tata cara ini berhasil membentuk Terung Rimbang poliploid yang terdiri dari 2x+3x dan 3x+4x. Jumlah individu poliploid diperkirakan sebanyak 59%, sisanya 41% merupakan diploid. Planlet yang memiliki sel tetraploid sekaligus ploidinya tertinggi diperoleh dari pemeraman kolkisina selama 72 jam.
PENDUGAAN PARAMETER GENETIK KOMPONEN HASIL UNTUK SELEKSI TIDAK LANGSUNG TANAMAN PADI (ORYZA SATIVA L.) BERDAYA HASIL TINGGI Faiqon, M. Mujadid; Supriyanta, Supriyanta; Wulandari, Rani Agustina
Vegetalika Vol 6, No 2 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (508.828 KB) | DOI: 10.22146/veg.26168

Abstract

Seleksi langsung terhadap daya hasil seringkali sulit dilakukan karena sifat alami hasil yang kompleks dan besarnya pengaruh lingkungan terhadap hasil. Seleksi tidak langsung mendasarkan pada komponen hasil yang berkorelasi terhadap hasil dapat dilakukan sebagai solusi untuk masalah tersebut. Pendugaan parameter genetik dilakukan untuk memperoleh informasi mengenai komponen hasil yang dapat digunakan untuk meningkatkan hasil per hektar. Sembilan genotipe padi  diasumsikan  berdistribusi  random  ditanam  mengikuti  rancangan RCBD di dua lokasi, Klaten dan Sleman. Komponen varian dan kovarian yang diduga melalui ANOVA dan ANCOVA digunakan untuk menduga heritabilitas, korelasi genetik, koheritabilitas, dan rasio nilai harapan CRA/RA. Analisis lebih lanjut untuk korelasi genetik dilakukan dengan analisis lintas untuk memperoleh informasi tambahan  mengenai hubungan  antara hasil dengan komponen hasil. Tinggi tanaman, panjang daun bendera, umur berbunga, umur panen, bobot 100 butir biji, jumlah malai per rumpun, panjang malai, dan kerapatan malai diketahui memiliki heritabilitas tinggi (lebih dari 0,7) dan lebih tinggi dibandingkan heritabilitas hasil per hektar (0,55 di Klaten dan 0,42 di Sleman). Jumlah malai per rumpun memiliki korelasi genetik positif dan tinggi dengan hasil per hektar sementara panjang daun bendera dan umur berbunga memiliki pengaruh langsung positif dan tinggi terhadap hasil per hektar di lokasi Klaten. Bobot 100 butir biji dan panjang malai memiliki korelasi genetik yang positif dan tinggi terhadap hasil per hektar sementara tinggi tanaman dan jumlah biji per malai memiliki pengaruh langsung yang tinggi dan positif. Akan tetapi, tidak terdapat komponen hasil yang memiliki rasio nilai harapan CRA/RA lebih tinggi dari 1 yang menunjukkan bahwa tidak ada komponen hasil yang dapat meningkatkan kemajuan genetik hasil per hektar melalui seleksi tidak langsung. Seleksi langsung terhadap daya hasil lebih efektif dibandingkan dengan seleksi tidak langsung melalui komponen hasil tertentu.
PENGENDALIAN MUTU BENIH TEBU (SACCHARUM OFFICINARUM L.) MATA TUNAS TUNGGAL MENGGUNAKAN PENGATURAN KADAR LENGAS MEDIA SERBUK ABU SEKAM PADI Panuntun, Henricus Tegar; Taryono, Taryono; Wulandari, Rani Agustina
Vegetalika Vol 6, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.921 KB) | DOI: 10.22146/veg.25985

Abstract

Bibit tebu mata tunas tunggal memiliki umur simpan yang relatif pendek. Apabila tanpa perlakuan, daya kecambah mata tunas tunggal sudah menurun pada umur simpan 2 hari. Oleh karena itu, dibutuhkan metode penyimpanan yang tepat untuk memperpanjang umur simpan, sehingga kualitasnya masih baik saat akan ditanam di lahan petani. Penelitian ini bertujuan untuk mengetahui kadar lengas media simpan dan umur simpan tebu terbaik untuk mempertahankan mutu bibit tebu mata tunas tunggal. Penelitian ini dilakukan dengan menggunakan rancangan perlakuan faktorial 3 × 4 + 1 dalam rancangan lingkungan acak lengkap (RAL). Faktor pertama adalah media simpan yang terdiri dari media simpan serbuk abu sekam padi dengan penambahan kadar lengas media 0, 20, 40 dan 60 %. Faktor kedua adalah klon tebu yang terdiri dari klon Kidang Kencana, klon Bululawang, dan klon VMC. Pengamatan dilakukan pada umur simpan 0, 4, 8, 12 hari. Hasil penelitian menunjukkan bahwa terdapat interaksi antara media simpan dan klon yang digunakan pada  jumlah mata tunas mati, jumlah mata tunas tumbuh, indeks vigor (IV), tinggi tanaman, jumlah daun, diameter batang sedangkan pada pengamatan lainnya, media simpan dan klon tebu tidak memberikan pengaruh yang nyata.  Penyimpanan menggunakan media dengan kadar lengas 20% secara umum sedikit menurunkan kualitas benih. Klon VMC menunjukkan perkecambahan benih paling baik dilihat dari gaya berkecambah dan indeks vigornya. Dari pengamatan mutu benih diketahui penambahan kadar lengas mampu memperpanjang masa simpan hingga umur simpan 12 hari , sedangkan pada mutu bibit penambahan kadar lengas mampu memperpanjang masa simpan hingga 8 hari.
GENOMIC DNA EXTRACTION METHOD FROM MATURE LEAF OF LAI (Durio kutejensis Becc.) Handayani, Fitri; Wulandari, Rani Agustina; Murti, Rudi Hari
AGRIVITA, Journal of Agricultural Science Vol 38, No 1 (2016): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i1.659

Abstract

Lai (Durio kutejensis Becc.) is an indigeneous germplasm of Kalimantan which has some superiorities compared to its close-relative, durian (Durio zibethinus Murr.). Genetic exploration of lai is important to support its breeding program. According to rapid development in molecular biology, genetic exploration effort of lai will be easier. One of significant step in any molecular biology activities is DNA isolation to produce high quality DNA for further analysis. Leaves of lai, as other perennial crop, contain of high concen-tration of polysaccharides and polyphenol which will be co-extracted with the DNA. These compounds can interfere enzymes activities in subsequent molecular analysis. The aim of this study was to establish an optimal and effective DNA extraction method to obtain high-quality DNA from mature leaf of lai. An established extraction buffer and its modification were used in this study. The result showed that modification 4 could produce high quality DNA, and was considered to be the most effective DNA extraction method for mature leaf of lai.
Microsatellite Genetic Markers of Saccharrum spp., and Erianthus sp. on Their Hybrids Murianingrum, Mala; Taryono, Taryono; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 3, No 1 (2018): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.905 KB) | DOI: 10.22146/ipas.34673

Abstract

Progeny identification is the important step that should be done after hybridization. However, polyploidy, aneuploidy and the high chromosome segregation in sugarcane which results various phenotypic characteristics variation and environmental effects become limiting factors to identify the progenies based on morphological characteristic. Microsatellite as one of molecular marker which has codominance inheritance, multiallelic, abundant in the genome and does not influenced by environmental factor is the best tool to asses the crossing fidelity accurately. This research aimed to identify the possibility of genetic marker of Saccharum spp. and Erianthus sp. on their hybrid using microsatellite molecular marker. This study was carried out in Molecular Genetic laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) Malang, from August 2016 to July 2017. Eighty-six (86) F1 intraspecific and interspecific progeny, three commercial sugarcane varieties (PSJT941, PS881 and VMC7616) and two wild types (S. spontaneum dan Erianthus sp.) were assessed genetically by three microsatellite markers. Identification of microsatellite genetic markers was conducted by comparing the visualization band results from electrophoresis of each male and female parent through their progenies. All primers could identify Saccharum spp. and Erianthus sp. genetic markers. There were one to eleven Saccharum spp. and Erianthus sp. genetic markers could be identified such as 2-11 PS881-specific alleles; 2-3 VMC7616-specific alleles; 1-5 PSJT941-specific alleles; two S. spontaneum-specific alleles and 1-2 Erianthus-specific alleles. These findings could be used as the advance genetic marker of microsatellite in sugarcane breeding to asses the cross fidelity.
MICROSATELLITE GENETIC MARKERS OF SACCHARRUM SPP., AND ERIANTHUS SP. ON THEIR HYBRIDS Murianingrum, Mala; Taryono, Taryono; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 3, No 1 (2018): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (645.905 KB) | DOI: 10.22146/ipas.34673

Abstract

Progeny identification is the important step that should be done after hybridization. However, polyploidy, aneuploidy and the high chromosome segregation in sugarcane which results various phenotypic characteristics variation and environmental effects become limiting factors to identify the progenies based on morphological characteristic. Microsatellite as one of molecular marker which has codominance inheritance, multiallelic, abundant in the genome and does not influenced by environmental factor is the best tool to asses the crossing fidelity accurately. This research aimed to identify the possibility of genetic marker of Saccharum spp. and Erianthus sp. on their hybrid using microsatellite molecular marker. This study was carried out in Molecular Genetic laboratory, Indonesian Sweetener and Fiber Crops Research Institute (ISFCRI) Malang, from August 2016 to July 2017. Eighty-six (86) F1 intraspecific and interspecific progeny, three commercial sugarcane varieties (PSJT941, PS881 and VMC7616) and two wild types (S. spontaneum dan Erianthus sp.) were assessed genetically by three microsatellite markers. Identification of microsatellite genetic markers was conducted by comparing the visualization band results from electrophoresis of each male and female parent through their progenies. All primers could identify Saccharum spp. and Erianthus sp. genetic markers. There were one to eleven Saccharum spp. and Erianthus sp. genetic markers could be identified such as 2-11 PS881-specific alleles; 2-3 VMC7616-specific alleles; 1-5 PSJT941-specific alleles; two S. spontaneum-specific alleles and 1-2 Erianthus-specific alleles. These findings could be used as the advance genetic marker of microsatellite in sugarcane breeding to asses the cross fidelity.
CALLUS INDUCTION ON TRUE SHALLOT SEED EXPLANT USING A COMBINATION OF BA AND 2,4-D Dharmayanti, Krisna; Sulistyaningsih, Endang; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 2, No 3 (2017): December
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4176.4 KB) | DOI: 10.22146/ipas.26276

Abstract

BA and 2,4-D combination were commonly used for in vitro culture of Shallot (Allium cepa L. var agregatum 2n = 2x = 16) to induce callus, but there was no information for callus induction on shallot seed (TSS) explant. Callus could be utilized for in vitro selection and generating of genetic variation. The aims of the research was to identify the response of TSS (Trisula and Tuk Tuk) as explant  and to obtain the optimum combination of BA and 2,4-D (mg.L-1): (0?0, 2?1, 2?2, 2?3, and 2?4) in callus induction. The research had been carried out in the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Gadjah Mada during the year 2015-2016. Factorial treatments of variety and growth regulators were arranged in Completely Randomized Design with four replications. Data of percentage of germination, shoot height, root length, percentage of callus formation, callus weight, and chromosomes number of callus were recorded. The results showed that combination of 2 mg.L-1 BA + (1? 4) mg.L-1 2,4-D induced callus formation on TSS but inhibits shoots and roots growth. The best callus proliferation was at a concentration of 1 mg.L-1 2,4-D. Tetraploid callus chromosomes (2n = 4x = 32) was detected in Trisula grown in the 2 mg.L-1 BA + 4 mg.L-1 2,4-D, but in the Tuk Tuk callus did not detected the changing of chromosomes number.
GENETIC VARIATION IN SELECTED INDIVIDUALS BASED ON NUMBER OF CAPSULE IN M5 SESAME MUTAN LINES DETECTED BY RAPD Kholida, Masruru; Taryono, Taryono; Wulandari, Rani Agustina
Jurnal Ilmu Pertanian Vol 3, No 2 (2018): August
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7049.749 KB) | DOI: 10.22146/ipas.32699

Abstract

Sesame (Sesamum indicum L.) is major oilseed crops with advantages in health and food industry. Due to self pollinated crop, breeding program in sesame utilized gamma rays irradiation to increase genetic variation. The research material consisted of 164 genotypes from 22 selected individual mutant line based on the number of capsules in M5 generation. This study is to detect genetic variation in selected individuals based on number of capsules in M5 generation of sesame mutant line using RAPD markers. The analysis consists of percentage of polymorphic loci, analysis of molecular variance and visualized in cluster and co-ordinate analysis. Fifteen primers RAPD were able to amplified 237 loci. Each genotype in populations had the similarity coefficient of 0.29 ? 0.85. Variance within selected individual line (66%) was higher than variance among selected mutant lines (34%). Variance in each selected individual line contributed to its high value. Line 34 showed the lowest polymorphism (23.21%) and line 19 depicted the highest polymorphism (61.60%).  
Genetic Variability, Standardized Multiple Linear Regression and Principal Component Analysis to Determine Some Important Sesame Yield Components Aristya, Vina Eka; Taryono, Taryono; Wulandari, Rani Agustina
AGRIVITA, Journal of Agricultural Science Vol 39, No 1 (2017): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i1.843

Abstract

Sesame is an important commodity in supporting various industries such as low saturated fat oil producing and are often able to adapt under stressed grown conditions. Breeding sesame is undertaken to increase production and is possible by radiation induced polygenic characteristic changes with a gamma rays source. The study aims to identify the effectiveness of genetic variability, standardized multiple linear regression, and principal component analysis to determine some important sesame yield components for indirect selection. Eighteen sesame mutant lines (black and white types) were studied for eleven quantitative traits. Two sesame types were irradiated with eight doses (100-800 Gy) of gamma rays individually. Variability studies on seed yield and yield components are important raw material of high productivity for all studied traits. Standardized multiple linear regression analysis is the most effective way to provide information of relationship between seed yield and yield components in sesame mutant lines for indirect selection.