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PHYLOGENETIC ANALYSIS AND VIRULENCE-CODING GENES CHARACTERIZATION OF NEWCASTLE DISEASE VIRUS ISOLATED FROM LAYING HEN BRAIN WITH TETELO SYMPTOM Sarah, Safira Iqlima; Nugroho, Christian Marco Hadi; Kurnia, Ryan Septa; Wardhana, Dhandy Koesoemo; Puspitasari, Heni
Jurnal Kedokteran Hewan Vol 13, No 3 (2019): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.298 KB) | DOI: 10.21157/j.ked.hewan.v13i3.14493

Abstract

Newcastle disease (ND) is an acute poultry disease caused by Paramyxovirus group. It has characteristic neurological symptoms, called torticollis. The molecular assay to find out the presence of viral genes in the brain can be an option in detecting ND virus infections since it penetrates brain barrier system. The purpose of this study was to identify ND viruses in the brain of chickens with torticollis symptoms, to analyze its phylogenetic and to characterize its virulence genetic code. Samples used were 12 dead chickens with historically had torticollis symptoms, obtained from poultry farms at several areas in West Java and Banten. Chicken brains were prepared for reverse transcriptasepolymerase chain reaction (RT-PCR) test. All positive samples then sequenced to obtain its nucleotide sequences from some of Fusion (F) genes analyzed its phylogenetic by comparing with Indonesian ND isolate virus from GenBank using Mega X software. The results of RT-PCR test showed that only one sample (Virus MSL.03) contained genes of ND virus. Based on homology tests and phylogenetic analysis, the virus belonged to subgenotype VIIh with an identical level of 95.34-95.86% when compared to several isolates from Indonesia. The MSL.03 ND virus has 112RRRKRF117 pattern in F0 indicatest its virulent category.
PENGEMBANGAN BAHAN AJAR STATISTIKA BERBASIS MEDIA INTERAKTIF UNTUK SISWA SMK AKUNTANSI DI LAMPUNG BARAT Puspitasari, Heni; Koestoro, Budi; Caswita, Caswita
Jurnal Teknologi Informasi Komunikasi Pendidikan Vol 3, No 5 (2015): Jurnal Teknologi Informasi Komunikasi Pendidikan
Publisher : Jurnal Teknologi Informasi Komunikasi Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purposes of this study were, (1) to describe the conditions and the potential of the instructional materials development ; (2) to describe the process of the development of interactive media ; (3) to test the efficiency, the attractiveness and effectiveness of the use of media in learning. This research was using the method of development, with the subjects of accounting vocational students in West Lampung. Data were collected through observation, questionnaires, tests and analyzed descriptively and t-test examination. Conclusions of research were : (1) the conditionsand the potential for learning was not optimal, instructional material of interactive media was needed by students ; (2) this interactive media development process involued a computer expert, instructional design and material substance ; (3) the efficiency of product was 80,55; the attractiveness of product was 79,02% and the effectiveness of product was 3,60.Penelitian ini bertujuan, (1) mendeskripsikan kondisi dan potensi pengembangan bahan ajar ; (2) mendeskripsikan proses pengembangan media interaktif; (3) menguji efisiensi, kemenarikan dan efektivitas penggunaan media dalam pembelajaran. Penelitian dengan metode pengembangan,  dengan subyek siswa SMK Akuntansi di Lampung Barat. Data dikumpulkan melalui observasi, angket,tes dan dianalisis secara deskriptif dan uji t-test. Kesimpulan penelitian adalah: (1) kondisi dan potensi pembelajaran belum optimal, bahan ajar yang dibutuhkan siswa adalah media interaktif ; (2) proses pengembangan media interaktif ini divalidasi ahli komputer, desain pembelajaran dan substansi materi ; (3) efisiensi produk sebesar 80,55 ; kemenarikan produk sebesar 79,02% dan efektivitas produk sebesar 3,60.Kata kunci : bahan  ajar, media interaktif, statistika
THE IMMUNOSTIMULAN POTENTIAL OF TENGGULUN (PROTIUM JAVANICUM) LEAVES TOWARDS T CELL CD4+ AND IFNγ SECRETION ON PBMC CHICKEN Jayawardhana, Andi; Puspitasari, Heni; Meles, Dewa Ketut; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol 6, No 3 (2016)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.478 KB) | DOI: 10.20473/ijtid.v6i3.2992

Abstract

One of the plants with immunostimulant activity is Tenggulun leaves which contain of flavonoid, like terpenoid. The aim of this research is to find the potential of Tenggulun’s leaves extract to have the immunostimulant activities. The potential of immunostimulant activity is identified by the increasing the amount of T-cell CD4+ expression and IFNγ secretion. The research method is conducted through cultured chicken PBMC which is infected by ND virus; it is then treated with Tenggulun’s leaves extract with immunostimulant. The result of immunocitochemistry examination CD4+ secretion on PBMC cultures shows how tenggulun is significantly different from the control in the secretion CD4+. The 10μg of tenggulun extract can modulate the T cell CD4+ secretion 68.8±0.83. It is significantly different from K (control) (p<0,05) and treatment group K+, P0, and P1. The examination of IFNγ level using ELISA from tenggulun leaves extract of 10μg doses were inoculated after being infected by ND virus contained immonostimulant potential in increasing the secretion of IFNγ 120.91±6.44. It is significantly different from K-, K+, and P1, yet not significantly different from P0. The content of terpenoid can increase IFNγ secretion on the macrofag cells culture and limfosit cells. 
PRODUCTION AND CHARACTERIZATION OF IMMUNOGLOBULINE YOLK AS ANTI ANTIGEN MEMBRANE TOXOPLASMA GONDII Puspitasari, Heni; Praptiwi, Yuliana; Suwanti, Lucia
Indonesian Journal of Tropical and Infectious Disease Vol 6, No 2 (2016)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.802 KB) | DOI: 10.20473/ijtid.v6i2.1365

Abstract

Toxoplasma gondii is an obligate parasite intracellular which can infected human and other mammalian. Immunoglobulin Y technology  offers several advantages better than antibody production in mammals. This research was aimed to get immunoglobulin Y from egg yolk, to prove that antibody against membrane T. gondii antigen can produced from immunoglobulin Y and to know the characterization of  immunoglobuline Y according to  molecular weight by SDS PAGE and reactivation of  antibody antigen  by Western Blott. This research devided  from many step : passase tachyzoites T. gondii into mice by peritoneal infection, cultivated  the tachyzoite from intraperitoneal fluid, preparation of  membrane antigen tachyzoite T. gondii, then  immunization laying hens with membrane antigen, extraction and purification immunoglobuline Y from egg yolk and then protein analyzed by SDS PAGE and Western Blott.  The result of this resarch showed that immunoglobulin Y from egg yolk can  produced antibody against protein membrane T. gondii. The result of analyzed profile protein immunoglobuline  Y according SDS PAGE  has molecular weight 179,8 kDa. Analyzed from Western Blott showed that immunoglobulin Y can recognize antigen epitope of  T. gondii on molecular weight 35,7 kDa and 78,8 kDa.