Cut Nila Thasmi, Cut Nila
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ISOLASI DAN KARAKTERISASI PROTEIN SPESIFIK PREGNANCY ASSOCIATED SUBSTANCE (PAS) SERUM SEBAGAI KANDIDAT UNTUK TEST KEBUNTINGAN DINI PADA SAPI LOKAL Dasrul, Dasrul; Thasmi, Cut Nila; Harris, Abdul
Jurnal Kedokteran Syiah Kuala Vol 6, No 2 (2006): Volume 6 Nomor 2 Agustus 2006
Publisher : Universitas Syiah Kuala

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Abstract

Abstrak.  Penelitian   ini  bertujuan   untuk  rnengetahui   karakterisasi   protein   PAS  serum  sapi  bunting sebagai  kandidat  bahan  aktif  untuk  test  kebuntingan   dini  pada  sapi  Aceh, Penelitian   ini  merupakan penelitian    eksperimental     laboratorium    dengan    menggunakan     serum   darah   sapi   bunting    yang diperoleh   dari  petemakan   masyarakat   disekitar   Darussalam-Banda    Aceh.  Preparasi   dan  ekstraksi protein  PAS dilakukan   dengan  mengunakan   metode  sonikasi  dan identifikasi   protein  PAS dilakukan dengan   elektroferosis     rnelalui   SDS-PAGE    dan   Western   bloting.    Sedangkan    uji   kadar   protein dilakukan   rnelalui metode biuret.  Hasil  penelitian  menunjukan  bahwa  serum  darah  sapi bunting  dapat memproduksi   epitop  protein  PAS,  dengan  berat  molekul  protein  PAS    sebesar  65, 15 kDa.   Protein PAS  serum  darah  dapat   menginduksi   antibodi   terhadap   PAS  yang  efektif  sebagai   bahan  bioaktif deteksi  kebuntingan  dini pada sapi Aceh.  (JKS  2006; 2:59-68)  Kata Kunci: kotiledon  sapi aceh,  protein  PAS,  anti-PAS,  deteksi  dini kebuntinganAbstract.    This research  was aimed  to study  the characteristic   of PAS protein  of serum as a candidate of active  material  for early  detection   of cow  pregnancy.   This  study  was  conducted  as a  laboratory experimental   by using  serum  of pregnant  cow,  which  was  obtained  from  livestoxk  in Darussalam- Banda   Aceh.   Preparation   and  extraction    of  PAS   protein  ··were  performed    using  sonication   and identification    of  PAS   protein,   by  SDS-PAGE   electrophoresis    and  Western   blotting.   The  results showed   that  serum  is able  to produce   the epitope  of PAS protein.  The  weight  of molecule  of PAS protein  epitope  was  6~, 15 kDa.  PAS  protein  of serum  could  cause  an induction  of antibody  to PAS, which   is effective  as a bioactive  material  for early detection  of cow pregnancy.  (JKS 2006;  2:59-68)Keywords:  serum Aceh  bovine, protein  PAS, Anti PAS  and early pregnant
THE IMPROVEMENT OF SEMEN QUALITY AND TESTOSTERONE LEVEL OF BALI CATTLE AFTER PROSTAGLANDIN F2α ADMINISTRATION Sari, Eka Meutia; Tanjung, Satria; Sari, Dewi Ratna; Akmal, Muslim; Siregar, Tongku Nizwan; Thasmi, Cut Nila
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): J. Ked. Hewan
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i4.14821

Abstract

This study aimed to determine the effect of prostaglandin F2? (PGF2?) administration on improving semen quality and testosterone levels on Bali cattle. This study used 3 Bali cattle aged 2, 3.5, and 5 years old. The sample used in this study was Bali cattle semen. In this study, the Latin Square Design was used with three different treatments were administered over three time periods. The treatments performed were P1 (5 mL physiological NaCl), P2 (25 mg prostaglandin), and P3 (37.5 mg prostaglandin) while treatment period was three weeks of treatment administration, which were 1st. 2nd, and 3rd week. Blood collected through coccygea vein 30 minutes after treatment to measure testosterone levels using enzyme-linked immunosorbent assay (ELISA) technique. Statistical analysis showed that PGF2? administration at a dose of 25 mg and PGF2? at a dose of 37.5 mg had no effect (P 0.05) on volume, color, pH, consistency, concentration, and motility of Bali cattle spermatozoa. The volume of semen obtained from P1; P2; and P3 were 6.5±0.9; 6.3±0.6; and 6.2±1.2 mL, respectively. The color of the semen in P1, P2, and P3 were beige and milky white. The pH in groups P1; P2; and P3 were 6.7±0.6; 7.1±0.2; and 6.5±0.2, respectively. Spermatozoa concentration in P1; P2; and P3 were 1,328±96.43 x 106 cells/mL; 1,354±102.19 x 106 cells/mL; and 1,353.3±88.55 x 106 cells/mL, respectively. Spermatozoa motility in P1; P2; and P3 were 73.3±2.8%; 71.6±2.8%; and 73.3±2.8%, respectively. Testosterone levels in P1 and P3 were 5.05±0.22 and 6.74±1.38 ng/mL, respectively (P 0.05). Based on the results of this study, it was concluded that the administration of PGF2? does not affect semen quality but can increase the level of testosterone on Bali cattle.
PHYSICAL PROPERTIES OF CERVICAL MUCUS OF REPEAT BREEDER ACEH CATTLE Siregar, Tongku Nizwan; Agustina, Iin; Masyitah, Dian; Azhar, Al; Dasrul, Dasrul; Thasmi, Cut Nila; Sulaiman, Rusli; Daud, Razali
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.3.378

Abstract

This study aimed to investigate the changes in physical properties of cervical mucus of repeat breeder aceh cattle. Subjects were three normal (K1) and repeat breeder (RB) adult female aceh. K1 were post partus cattle >2 months had successful pregrncy by one insemination and had regular cycle twice whereas K2 consisted of repeat breeders, i.e. cattle had normal estrus but failed to be pregnant after three times artificial inseminations. Cervical mucus samples, 50 ml, were collected by aspiration using a sterile catheter and disposable syringe. Catheter was inserted via vagina followed by rectal palpation to direct catheter reached the cornua uterus or vaginal folds. Result of evaluation on the physical properties of cervical mucus from fertile and repeat breeder cattle showed that their cervical mucus was unavailable/small in quantity (0.00 vs. 66.67%), cloudy color (0.00 vs. 66.67%), thick consistency (0.00 vs. 100%), fern pattern (0.00 vs 66.67%), spinnbarkeit (5.16±1.60 vs. 2.83±2.02 cm) and pH values (7.33±0.57 vs 9.33±1.52). It can be concluded that cervical mucus of repeat breeder aceh cattle were lesser in quantity and had cloudier color, thicker consistency, and lower pH than that of fertile aceh cattle.
DIAGNOSIS KEBUNTINGAN DINI PADA KAMBING PERANAKAN ETAWAH (Capra hircus) DENGAN MENGGUNAKAN HARNESS DAN CRAYON (Early Pregnancy Diagnosis in Etawah Cross Goat (Capra hircus) Using Harness and Crayon) Prabudi, Tomi; Riady, Ginta; Azhar, Azhar; Dasrul, Dasrul; Thasmi, Cut Nila; Winaruddin, Winaruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 3 (2017): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.734 KB) | DOI: 10.21157/jim vet..v1i3.3292

Abstract

ABSTRAK Penelitian bertujuan mengetahui efektivitas alat Harness dan Crayon yang digunakan sebagai alat diagnosis kebuntingan dini pada kambing peranakan Etawah (Capra hircus). Penelitian ini menggunakan 10 ekor kambing betina dalam masa laktasi dengan berat badan rata-rata 30 kg dan kisaran umur 2-2,5 tahun dan 1 ekor kambing jantan dengan berat badan sekitar 70 kg dan kisaran umur 4-4,5 tahun. Sepuluh ekor kambing betina pada penelitian ini dikawinkan secara alami dengan 1 ekor kambing jantan yang sudah dikenakan Harness dan Crayon selama 30 hari periode kawin. Diagnosis kebuntingan dini menggunakan Harness dan Crayon dilakukan pada hari ke 18-22 setelah perkawinan. Akurasi diagnosis kebuntingan dini dengan Harness dan Crayon dikonfirmasi menggunakan alat Ultrasonography (USG) pada hari ke-35 setelah perkawinan. Tujuh dari sepuluh ekor kambing betina mengalami kawin alami yang ditandai adanya warna Crayon pada punggungnya. Hasil diagnosis kebuntingan dini menggunakan Harness dan Crayon mencapai 100% (7 ekor kambing betina tidak kawin kembali) selama 5 hari pengamatan tidak kembali berahi (Non-Return to Estrous Rate). Tingkat akurasi diagnosis kebuntingan dini dengan Harness dan Crayon menurun menjadi 85,7% setelah dikonfirmasi status kebuntingan kambing dengan alat USG. Alat Harness dan Crayon memiliki tingkat akurasi yang tinggi sebagai penetapan status positif bunting pada kambing betina peranakan Etawah. Kata kunci: Kambing PE (Capra hircus), Harness dan Crayon, Ultrasonography (USG).ABSTRACT             This study aimed to determine the effectiveness of harness and crayon as an early pregnancy diagnosis tool for Etawah cross goat (Capra hircus). This study used 10 lactating female goats with average weight was 30 kgs and the ages ranging from 2-2.5 years old and one male goat with weight of 70 kgs and the age of 4-4.5 years old. Ten female goats in this study were mated naturally with the male goat, while wearing harness and crayon during 30 days of mating period. Pregnancy diagnosis was carried out on days 18-22 postservice. Seven out of ten female goats were marked with crayon on their backsas on indication of mating. Pregnancy status was subsequently confirmed through USG on day 35 after mating. Early pregnancy diagnosis using harness and crayon showed 100% NRR. Accuracy of harness and crayon as pregnancy diagnosis tool decreased until 85.7% following USG confirmation. The harness and crayon has a high accuracy rate for early pregnancy diagnosis means for pregnantly status in etawah goats. Keyword: Etawah cross goat (Capra hircus), harness and crayon, ultrasonography (USG).
JUMLAH CEMARAN Escherichia coli PADA DAGING AYAM BROILER DI PASAR RUKOH, BANDA ACEH Selfiana, Dwi Rosa; Rastina, Rastina; Ismail, Ismail; Thasmi, Cut Nila; Darniati, Darniati; Muttaqien, Muttaqien
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 2 (2017): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.002 KB) | DOI: 10.21157/jim vet..v1i2.2708

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ABSTRAK      Penelitian ini bertujuan untuk mengetahui jumlah cemaran Escherichia coli pada daging ayam broiler di Pasar Rukoh, Banda Aceh. Sampel penelitian yang digunakan adalah 4 paha dan 4 punggung  daging ayam broiler. Pengambilan sampel dipilih secara acak pada empat pedagang Pasar Rukoh, Banda Aceh. Setiap pedagang diambil 2 sampel bagian daging ayam broiler sekitar punggung dan paha atas dan dimasukkan ke dalam plastik yang steril lalu masukkan ke dalam Cooler box. Metode pengujian yang dilakukan mengacu pada Bacteriological Analytic Manual dan Standar Nasional Indonesia (SNI) 01-2897-2008. Data yang diperoleh dianalisis secara deskriptif. Nilai  cemaran Escherichia coli terendah 2,6 x 102 cfu/gr dan nilai  tertinggi cemaran yaitu 5,2 x 102 cfu/gr. Semua sampel daging ayam telah tercemar Escherichia coli, yang memperlihatkan perubahan koloni berwarna metalik kehijauan pada media Eosin Methylen Blue Agar (EMBA). Dari penelitian ini dapat disimpulkan, bahwa jumlah cemaran Escherichia coli pada daging ayam broiler di Pasar Rukoh, Banda Aceh melebihi batas maksimum cemaran mikroba. Menurut SNI 01-7388-2009 bahwa batas maksimum cemaran mikroba (BMCM) Escherichia coli pada daging ayam segar adalah kurang dari 1 x 101 cfu/gr.Kata kunci: Esherichia coli, daging ayam broiler, Pasar Rukoh.                                              ABSTRACT      This study aims to determine the level of Escherichia coli contamination in chicken meat sold in Rukoh traditional market, Banda Aceh. The samples were four thighs and four back parts of chicken meat. Sampling was collected randomly in four stores There were 2 samples taken from each store. Testing methods refers to the Bacteriological Analytical Manual and SNI 01-2897-2008. The data then analyzed descriptively. Escherichia coli contamination at the lowest value is 2.6 x 102 cfu / g and the highest contamination is 5.2 x 102 cfu / g. All samples of chicken meat has been contaminated with Escherichia coli, these results of contamination marked by the metallic green colonies on Eosin Methylen Blue Agar (EMBA). Thus, the author can conclude that the number of Escherichia coli contamination in chicken meat sold in Rukoh traditional market, Banda Aceh exceeds the maximum limit of microbial contamination. According SNI 01-7388-2009 that the maximum limit of Escherichia coli contamination (BMCM) allowed on fresh chicken meat is less than 1 x 101cfu / g.Keywords: Esherichia coli, Chicken meat, Rukoh traditional market.
EFEK PEMBERIAN AMPAS KEDELAI NONFERMENTASI DAN YANG DIFERMENTASI Aspergillus niger TERHADAP JUMLAH FOLIKEL TELUR AYAM KAMPUNG (Gallus domesticus) Putri, Yohanna; Thasmi, Cut Nila; Adam, Mulyadi; -, Nurliana
Jurnal Medika Veterinaria Vol 7, No 2 (2013): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.346 KB) | DOI: 10.21157/j.med.vet..v7i2.2934

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Penelitian ini bertujuan membandingkan efek pemberian ampas kedelai nonfermentasi dan yang difermentasi Aspergillus niger terhadap jumlah folikel telur ayam kampung (Gallus domesticus). Hewan yang digunakan dalam penelitian ini adalah 15 ekor ayam kampung betina umur 16 bulan dengan berat badan berkisar 1,2-1,7 kg dan dibagi dalam 2 kelompok  perlakuan. Masing-masing kelompok perlakuan terdiri atas 5 ekor ayam kampung. Kelompok I (P0) sebagai kontrol diberi pakan komersil 324-2 sebanyak 100 g/ekor/hari dan Kelompok II (P1) diberi pakan komersil sebanyak 85 g + ampas kedelai non fermentasi sebanyak 10 g/ekor/hari, dan Kelompok III (P3) diberi pakan komersil sebanyak 85g + ampas kedelai fermentasi sebanyak 10 g/ekor/hari, dan air minum diberikan secara adlibitum. Masing-masing kelompok diberi perlakuan selama 30 hari berturut-turut. Pada hari ke-31, ayam dibedah untuk melihat jumlah folikel telur pada masing-masing kelompok perlakuan. Rata-rata (±SD) jumlah folikel telur pada  P1 dan P2 berturut-turut adalah 15±27,5 dan 23,2±16,7 folikel. Hasil penelitian menunjukkan bahwa jumlah folikel telur ayam kampung yang diberi ampas kedelai fermentasi lebih tinggi dan berbeda nyata (P<0,05) dari kelompok yang diberi ampaskedelai nonfermentasi
PENGARUH METODE PENCUCIAN SPERMATOZOA SAPI ACEH TERHADAP MOTILITAS, PERSENTASE HIDUP, DAN INTEGRITAS MEMBRAN PLASMA UTUH SPERMATOZOA (The Infuence of Aceh Bull Spermatozoa Washing Method on Spermatozoa Motility and Plasma Membrane Integrity of Intact Spermatozoa) Handayani, Listin; Dasrul, Dasrul; Akmal, Muslim; Thasmi, Cut Nila; Hamdan, Hamdan; Adam, Mulyadi
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.844 KB) | DOI: 10.21157/j.med.vet..v9i2.3808

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This study aimed to determine the effect of sperm washing by swim up and centrifugation in isotonic medium on sperm quality of aceh bull. In this study, fresh semen from healthy male aceh bull aged 3-4 months was collected using artificial vagina. Immediately after semen collection, fresh semen quality was examined macroscopically and microscopically. Subsequently, sperm washing was performed by centrifugation and swim up in sperm washing medium. Group 1 (P0) as control group, cement washed with isotonic solution (andromed medium: saline solution) with ratio of 1:8. 2. Group 2 (P1), cement was separated by centrifugation method, group 3 (P2), all cement was separated by swim up method then examined the sperm quality sperm washing results. Each treatment was repeated 5 times. Quality parameters measured were the percentage of spermatozoa motility, sperm viability, and plasma membrane integrity intact spermatozoa. Data were analyzed with analysis of variance one-way pattern, followed by Duncans multiple test. The results showed the mean ± SD percentage of sperm motility of each treatment group (P0; P1; P2) respectively amounted to 72.00±3.74, 66.40±4.77, and 73.60±3.29%. The percentage of viability was 72.00 ±3.74%, 66.40±2.88%, 71.80±2.17%. The percentage of plasma membrane integrity is intact spermatozoa was 68.20±1.79%, 57.20±3.77%, 69.00±2.00%. Results of this study showed that the percentage of motility, live spermatozoa and plasma membrane integrity intact after separation by swim-up method were significantly different (P <0.05) compared with no separation.Key words: spermatozoa quality, aceh bulls, centrifugation, swim up
PENGARUH PEMBERIAN PMSG DAN HCG TERHADAP PENINGKATAN JUMLAH KELAHIRAN HAMSTER CAMPBELL (Phodopus campbelli) (Effect of PMSG and hCG to Increase Birth Number in Campbell’s Hamsters (Phodopus campbelli)) Afriani Nur, Novi Afriani Nur; Siregar, Tongku Nizwan; Hamdan, Hamdan; Nasution, Idawati; Thasmi, Cut Nila; Dasrul, Dasrul
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.447 KB) | DOI: 10.21157/j.med.vet..v10i1.4041

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The aim of this study was to determine the effect of superovulation induction through Pregnant Mare’s Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (hCG) injection to increased birth number in Campbell’s hamsters (Phodopus campbelli). This study used 10 unpregnant female Campbell’s hamster, aged 4-6 months, weighing of 25-30 grams. Hamsters were divided into K1 and K2, each consist of five hamsters. K1 injected with 1 ml physiologic NaCl, while K2 intraperitoneally injected with PMSG and hCG in 5 IU. Mating was held after injection. Male and female hamsters were put in one box with ration of 1:1. The observation of vagina plug was done in order to confirm whether the mating successes or not. The average amount of fetus birth through superovulation on K1 and K2 were 3.40±1.3 and 7.80±1.1, respectively. In conclusion, the superovulation induction using the combination of PMSG and hCG increase birth number on Campbell’s hamsters.
ANGKA KEBUNTINGAN SAPI LOKAL SETELAH DIINDUKSI DENGAN PROTOKOL OVSYNCH (Conception Rates of Local Cows after Induction with Ovsynch Protocols) Efendi, Mefrianti; Siregar, Tongku Nizwan; Hamdan, Hamdan; Dasrul, Dasrul; Thasmi, Cut Nila; Razali, Razali; Sayuti, Arman; Panjaitan, Budianto
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.024 KB) | DOI: 10.21157/j.med.vet..v9i2.3804

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This study aims to know the effect of estrus synchronization using ovsynch method on local cow pregnancy rate. This study used 10 local cows without pregnant status and clinically healthy. All cows were divided into 2 treatment groups. Cows in group I (K1) were synchronized estrus using 5 mg/ml PgF2α intramuscularly with multiple injection patterns with intervals of 10 days. Group II (KII) was synchronized by ovsynch protocol, initiated with injection of 1ml GnRH on day-0. On the 7th day, the whole cows in K II were injected with 2 ml PgF2α then followed by re-injected with 1 ml of GnRH on the 9th day. Artificial insemination was performed 24 hours after GnRH last injection. The data were analyzed descriptively. The results showed that 3 cows in group I were diagnosed pregnant (60%), whereas in group II, 4 cows werediagnosed pregnant (80%). In conclusion, the estrus synchronization with ovsynch protocols provide a higher pregnancy rate than the conventional estrus synchronization.Key words: synchronization, ovsynch, GnRH, PgF2α
HUBUNGAN ANTARA INTENSITAS ESTRUS DENGAN KONSENTRASI ESTRADIOL PADA SAPI ACEH PADA SAAT INSEMINASI (Relation between Estrous Intensity and Estradiol Concentration on Local Cattle during Insemination) Ramli, Mauridatun; Siregar, Tongku Nizwan; Thasmi, Cut Nila; Dasrul, Dasrul; Wahyuni, Sri; Sayuti, Arman
Jurnal Medika Veterinaria Vol 10, No 1 (2016): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.507 KB) | DOI: 10.21157/j.med.vet..v10i1.4032

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This study aims to determine the relationship between the intensity of estrus and estradiol concentrations during insemination on local cattle. Ten cows were divided into two groups with each group consist of five cows with category: aged 5-8 years, weight 150-250 kg, clinically healthy, and not pregnant. Plasma samples for examination of the hormone estradiol concentrations were collected during insemination time from jugular vein. Prior to the collection of plasma samples, whole cow were estrus synchronized using prostaglandin F2 alpha (PGF2α) and Ovsynch protocol. The PGF2α were injected twice at intervals of 11 days. Examination of estradiol hormone concentration was performed with enzyme-linked immunosorbent assay (ELISA) using estradiol commercial kits. The results of this study indicate that statistically there is no relationship between estrous intensity and estradiol concentration.