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KARAKTERISASI α-AMILASE DARI ASPERGILLUS VERSICOLOR 3A1 YANG DIPRODUKSI PADA MEDIA LIMBAH CAIR TAPIOKA Aini, Fitratul; Mubarik, Nisa Rachmania; Manaf, Lisdar A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.985 KB) | DOI: 10.24002/biota.v14i3.2581

Abstract

The aim of this experiment was to characterize A. versicolor 3a1 ?-amylase produced on cassava liquid waste media. Two types of media, base and combination media, were used as a comparison. Cassava liquid waste media contains 1% cassava starch, 1% yeast extract, 0.13% KH2PO4, and 0.05% MgSO4 diluted in cassava liquid waste. Base media contains same composition but using aquadest as a solvent, and combination media using mixture of aquadest and cassava liquid waste. A. versicolor 3a1 ?-amylase showed its maximum specific activity in cassava liquid waste, base, and combination media after 3, 7, and 4 days incubation, respectively. Crude extract of ?-amylase fromA. versicolor 3a1 was precipitated in 20-80% (w/v) ammonium sulphate. Precipitation of A. versicolor 3a1 ?-amylase with 70% (w/v) ammonium sulphate on cassava liquidwaste, 60% on base media, and 60% on combination media will increase its specific activity 16.6, 4.28, and 5.65 times, respectively, compared to the specific activities ofcrude before precipitation. ?-Amylase crude extract from A. versicolor 3a1 from all media showed its highest specific activity at 70oC and pH 5.0, and addition of FeSO4 increased the specific activity. Precipitated A. versicolor 3a1 ?-amylase from all media showed its highest specific activity at 70oC and pH 6.0. Addition of FeSO4 precipitated 3a1 ?-amylase from base and combination media will increase its specific activity, while MgSO4 will increase its specific activity in cassava liquid waste media. Thermostability assay revealed that the crude and the precipitated 3a1 ?-amylase were relatively stable at 70oC up to 180 minutes incubation, except for precipitated3a1 ?-amylase on cassava waste media. Crude ?-amylase 3a1 was relatively stable at pH 5-9 up to 1 hour incubation with wide pH ranges, while the precipitated with narrow pH ranges.
THE EFFECTIVITY OF BACTERIA ISOLATED FROM OF LIQUID WASTE PALM OIL PLANTATION ON GANODERMA BONINENSE Aini, Fitratul
International Journal of Ecophysiology Vol. 1 No. 1 (2019): International Journal of Ecophysiology
Publisher : Talenta Publisher

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Abstract

Ganoderma boninense is one of the main pathogenic fungus in oil palm plantations. Generally, these pathogen cause root rot (basal stem rot). Biological control that has been widely used reduce the infection is using bacteria. Liquid waste palm oil has potential to produce bacteria that is able to degrade Ganoderma boninense that causes root rot in oil palm. Liquid waste were obtained from Muaro Sabak Regency Jambi Province. Bacteri were isolated and cultivated in nutrient agar medium, characterized and identified for antagonistic test against G. boninense. Results showed that 16 bacterial isolates were identified, among of them are able to inhibit Ganoderma boninense.
PENGARUH PENGENDALIAN INTERN, PEMANFAATAN TEKNOLOGI INFORMASI, DAN KUALITAS SUMBER DAYA MANUSIA TERHADAP KETERANDALAN PELAPORAN KEUANGAN DAERAH (Studi Pada Satuan Kerja Perangkat Daerah Kota Payakumbuh) Aini, Fitratul; Tanjung, Amries Rusli; Anggraini, Lila
Jurnal Online Mahasiswa (JOM) Bidang Ilmu Ekonomi Vol 2, No 2 (2015): Wisuda Oktober 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Ilmu Ekonomi

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Abstract

This study aimed to examine the influence of the internal control, information technology utilization, and quality of human resources on the local government financial reporting reability. This study was conducted at SKPD of Payakumbuh city. The population in this study were employed who worked in the SKPD Payakumbuh city (25 government agencies). The sampling method used in this study is purposive sampling method. The respondent in this study is the head of SKPD, the head of financial, treasurer of SKPD and financial staff is a side direct related in the financial management area on government of Payakumbuh city. The sample used in this study were 75 respondents. The hypotheses then tested is multiple linear regression analysis by using SPSS version 20.0.The result of this study indicated that internal control and quality of human resources tend to have effect on local government financial reporting reability. While information technology utilization has no effect on local govermeny financial reporting reability. The magnitude of the effect (R2) internal control, information technology utilization and quality of human resources on the local government financial reporting reability was 35,9%. While the remaining 64,1% is influenced by other independent variabel that are not observed in this study.Keywords: Reporting, Control, Information Technology, and Human Resources.
PENGARUH PEMBERIAN PAKAN TAMBAHAN DARI KOMBINASI TEPUNG CACING TANAH DAN TEPUNG AMPAS TAHU TERHADAP PERTUMBUHAN IKAN BETOK (Anabas testudineus) Berlian, Zainal; Aini, Fitratul; Aliah, Dinatul
Biota Vol 1 No 1 (2015): Jurnal Biota 2015
Publisher : Faculty of Science and Technology State Islamic University Raden Fatah Palembang

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Abstract

soil Worm and knowing Waste to own womb very high protein until Soil Worm and knowing Waste can to be used as the good addition food to betoks fish. with fish age betok 4 month Soil Worm Giving and knowing Waste to be expected can to level it growing to Fish Betok. this Research have go to know the influence of Soil Worm-giving and Waste Know in Fish growing Betok (Anabas testudineus) and to know foodwhich is to get it result that maximal in Fish growing Betok (Anabas testudineus). the Research is executed in Laboratory TO MIPA UIN Raden Fatah Palembang . This Research to use it Random Planing Complete (RAL) with 4 Treatment and 6 Repeating. The mentioned Treatment is P0= pelet, P1= knowing waste 8 gr and soil worm 4 gram, P2= knowing Waste 6 gr and soil worm 6 gr, P3= Soil Worm 8 gr and knowing waste 4 gr. show analysiical Result that growing grew highest to long of fish to treatment P3=4,36 cm and lowest 1,96 cm. To highest heavy to treatment P3= 4,76 gr, and lowest 1,5 gr. Its conclusion is Soil Worm giving and Waste Know to fish Betok (Anabas testudineus) influency in long growing and heavy of body betoks fish
KARAKTERISASI DAN IDENTIFIKASI BAKTERI RIZOSFER TANAMAN SAWIT JAMBI Khairani, Khairani; Aini, Fitratul; Riany, Hesti
Al-Kauniyah: Jurnal Biologi Vol 12, No 2 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.108 KB) | DOI: 10.15408/kauniyah.v12i2.11723

Abstract

AbstrakBakteri rizosfer adalah bakteri yang terdapat pada daerah perakaran tanaman yang diketahui memiliki keanekaragaman tinggi. Bakteri rizosfer memiliki berbagai peran seperti menyediakan nutrisi bagi tanaman, melindungi tanaman dari infeksi bakteri patogen, menghasilkan hormon pertumbuhan seperti indol acetic acid, pelarut fosfat, pengikat nitrogen, dan lain-lain. Dengan berbagai kemampuan dan peran tersebut, maka perlu eksplorasi bakteri rizosfer tanaman sawit khususnya di daerah perkebunan agar diketahui kelompok bakteri rizosfer, sehingga dapat dimanfaatkan secara optimal. Penelitian ini bertujuan untuk mengetahui kelompok dan keragaman bakteri rizosfer pada tanaman sawit yang berumur 8, 11, dan 14 tahun. Penelitian dilakukan dengan pendekatan konvensional dengan karakterisasi morfologi dan uji biokimia. Dari umur-umur tersebut, didapatkan 18 isolat yang terdiri dari 11 genus, yaitu Bacillus, Azospirillum, Actinobacillus, Xanthobacter, Enterococcus, Paenibacillus, Klebsiella, Pseudomonas, Arthrobacter, Micrococcus, dan Streptococcus. Bakteri rizosfer tanaman sawit memiliki keragaman genus yang sama pada umur 8, 11, dan 14 tahun. Genus bakteri yang didapatkan dapat dimanfaatkan sebagai PGPR (Plant Promoting Rhizobacteria).Abstract Rhizosphere bacteria are bacteria found in root areas. Rhizosphere bacteria have various roles such as providing nutrients for plants, protecting plants from infection pathogenic bacteria, producing growth hormones such as indol acetic acid, phosphate solvents, nitrogen binders, and others. By having these various abilities and roles, it is necessary to explore the bacteria of oil palm plants, especially in the plantation areas so that the group of rhizosphere bacteria can be identified and then utilized optimally. This study aims to determine the group and diversity of rhizosphere bacteria in oil palm plants that are 8, 11, and 14 years old. The research was conducted conventionally by observing morphological and biochemical characterization. At those ages, 18 isolates were consisting of 11 genera, namely Bacillus, Azospirillum, Actinobacillus, Xanthobacter, Enterococcus, Paenibacillus, Klebsiella, Pseudomonas, Arthrobacter, Micrococcus, and Streptococcus through conventional methods. Rhizosphere bacteria in oil palm plants have the same genus diversity at the ages of 8, 11, and 14 years. The bacterial genus obtained can be used as PGPR (Plant Promoting Rhizobacteria).
AKTIVITAS ANTAGONISTIK BAKTERI SELULOLITIK ASAL RHIZOSFER KELAPA SAWIT (ELAEIS GUINEENSIS JACQ.) TERHADAP GANODERMA BONINENSE PAT. Nisa, Miratun; Aini, Fitratul; Maritsa, Hasna Ul
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v13i1.11704

Abstract

AbstrakPenyakit busuk pangkal batang pada kelapa sawit (Elaeis guineensis Jacq.) disebabkan oleh Ganoderma boninense Pat. Bakteri selulolitik dari rizosfer kelapa sawit dapat dijadikan agen hayati dalam menghambat pertumbuhan G. boninense. Penelitian ini bertujuan untuk mendapatkan bakteri selulolitik dari rizosfer kelapa sawit dan menentukan efektivitasnya dalam menghambat pertumbuhan G. boninense. Tahap penelitian meliputi pengambilan sampel dari rizosfer kelapa sawit, isolasi, pemurnian, uji aktivitas selulolitik, dan uji daya hambat terhadap G. boninense serta identifikasi isolat bakteri yang potensial. Indeks selulolitik bakteri ditentukan dengan pewarnaan congo red 0,1%. Aktivitas penghambatan dilakukan dengan menentukan persentase daya hambat bakteri dalam menghambat G. boninense. Uji efektivitas dengan membandingkan hasil uji T pada taraf 5%. Hasil menunjukkan bahwa dari rizosfer kelapa sawit diperoleh 19 isolat bakteri selulolitik dengan indeks selulolitik tertinggi 4,38 pada isolat LBS1. Berdasarkan uji T dari efektivitas bakteri selulolitik terhadap G. boninense menunjukkan 6 isolat bakteri (LBS3, LBS4, DBS1, DBS7, SBS2 dan SBS6) memiliki nilai efektif atau berpotensi sebagai antagonis dengan persentase daya hambat tertinggi sebesar 40,17% isolat DBS1 yang merupakan genus Flavobacterium.  Harapan kedepannya, bakteri selulolitik yang diperoleh dapat dijadikan agen biokontrol terhadap G. boninense.Abstract Basal stem root disease in oil palm tree (Elaeis guineensis Jacq.) is caused by Ganoderma boninense Pat. infection. Cellulolytic bacteria from rhizosphere can be used as agents to inhibit G. boninense growth as pathogenic fungi. Purpose of the research is to obtain cellulolytic bacteria from oil palm tree rhizosphere and to determine their effectiveness in inhibiting G. boninense growth. The research stages included sampling from the oil palm tree rhizosphere, isolation, purification, cellulolytic activity test and dual culture test against G. boninense and identification of effective isolates. The cellulolytic index of bacteria was determined by congo red 0.1%. Antagonistic test was conducted to determine persentation of isolate bacteria in inhibit G. boninense and effectiveness test by comparing result of T test at the level 5%. The results showed that there are 19 isolates of cellulolytic bacteria from oil palm tree rhizosphere with highest cellulolytic index 4.38 (isolates LBS1). Based on T test of the effectiveness cellulolytic bacteria against G. boninense showed 6 isolates (LBS3, LBS4, DBS1, DBS7, SBS2, and SBS6) had effective values or potential antagonists with highest percentage inhibition 40.17% isolate DBS1 genus of Flavobacterium. Hope that in the future, cellulolytic bacteria obtained can be used as biocontrol agents for G. boninense.
PENGARUH KADAR THIAMINE (VITAMIN B1) TERHADAP PERTUMBUHAN JAMUR TIRAM PUTIH (Pleurotus ostreatus) Munir, Munir; aini, Fitratul; Jariah, Siti
Biota Vol 2 No 2 (2016): Jurnal Biota 2016
Publisher : Faculty of Science and Technology State Islamic University Raden Fatah Palembang

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Abstract

Indonesia is one of the centers of biodiversity in particular areas of food, which one of them is from a species of white oyster mushroom (Pleurotus ostreatus). To improve the effectiveness and efficiency of production time associated with fulfilling the request of the oyster mushroom (Pleurotus ostreatus) is high in the market, the utilization of thiamine (vitamin B1) need to be investigated. This study aims to determine the levels of thiamine (vitamin B1), which is best used in the growth of oyster mushroom (Pleurotus ostreatus). This study used quantitative experiments and research design complete randomized design with four treatments and six times as many repetitions as well as research along with the observations made on 21 June to 11 July 2015 (in 20 days) in the House of White Oyster Mushroom Production Nurseries, Ogan Komering Ulu Timur (OKUT). As for the levels of thiamine (vitamin B1) used in this study use the unit ppm (parts per million), namely: B0 (control), B1 (0.1 ppm), B2 (0.2 ppm), B3 (0.3 ppm). Parameters measured were the growth of oyster mushroom (Pleurotus ostreatus) which include emerging buds time (day), the time of harvest (days), weight (grams), wide hood (cm), and stem length (cm). Conclusions from the study and based on data analysis (ANOVA) and F test showed that administration of thiamine (vitamin B1) does not provide an effective influence on the growth of oyster mushroom (Pleurotus ostreatus). 
UJI KADAR ALKOHOL PADA TAPAI KETAN PUTIH DAN SINGKONG MELALUI FERMENTASI DENGAN DOSIS RAGI YANG BERBEDA Berlian, Zainal; Aini, Fitratul; ulandari, Resti
Biota Vol 2 No 1 (2016): Jurnal Biota 2016
Publisher : Faculty of Science and Technology State Islamic University Raden Fatah Palembang

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Abstract

Alcohol is widely used in industry, such as a solvent, as a synthesis in the chemical industry and at the present time alcohol is also used to fuel cars. This study aims to determine the differences in the level of alcohol contained in Tapai white sticky rice and cassava with different doses of yeast. This research was conducted in the laboratory of Chemical Biology Education Studies Program Faculty of Tarbiyah and Teaching UIN Raden Fatah Palembang in August 2015. The method used in this study is the experimental method using a completely randomized factorial design consisting of two factors, namely the type of fermentation ingredients: (Tapai white sticky rice and cassava) and a dose of yeast (0.5%, 1% and 1.5%) with four replications. Data were analyzed by ANOVA followed by two lines and test Beda Distance Real Duncan (BJND) at the level of 1%. Based on the results of the analysis showed that the alcohol content of the type of material the value of F count = 82.14> F table = 8.29 and yeast dose of F count = 812.14> F table = 6.01. From the results of this study concluded that: 1) There is a very real difference to the alcoholic content of fermented glutinous white Tapai and cassava. 2) There is a very real effect of different doses of yeast against alcohol content.
AKTIVITAS ANTIFUNGI EKSTRAK DAUN KEMANGI (Ocimum americanum L.) TERHADAP FUNGI Fusarium oxysporum Schlecht Berlian, Zainal; Aini, Fitratul; Lestari, Weni
Biota Vol 2 No 1 (2016): Jurnal Biota 2016
Publisher : Faculty of Science and Technology State Islamic University Raden Fatah Palembang

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Abstract

Fusarium oxysporum Schlecht. a parasitic fungus that cause leaf wilt disease in plants. Meanwhile, basil (Ocimum americanum L.) is a plant that contains of the active compound in the form of phenols which have antifungal activity. This study aimed to test whether the extract of leaves of basil have antifungal activity againts Fusarium oxysporum Schlecht. and determine the optimum concentration to inhibit the growth of the fungus Fusarium oxysporum Schlecht. Antifungal test is done by using paper disc diffusion method. The study design used was a completely randomized design with 4 treatments and 6 replications. The treatment is K0 (0% w/v), K1 (5% w/v), K2 (10% w/v), and K3 (15% w/v). The results showed that the leaf extract of basil have antifungal activity against Fusarium oxysporum Schlecht. Inhibition zone on K0, K1, K2, and K3 are each 0,0 mm, 1,49 mm, 2,46 mm, and 2,01 mm. The optimum concentration of antifungal activity of extract of basil, namely the K2 concentration (10% w/v). Based on analysis of variance (ANOVA), the concentration of basil leaf extract provides significant differences (p > 0,05) on fungus Fusarium oxysporum Schlecht., where Fcount > Ftable is 4,5 > 3,1.
PERBANDINGAN HASIL BELAJAR MENGGUNAKAN MODEL PEMBELAJARAN KOOPERATIF TIPETWO STAY TWOSTRAY (TSTS) DENGAN DEMONSTRASIUNTUK MENINGKATKAN HASIL BELAJARBIOLOGI SISWA KELAS VII SMPADABIYAHPALEMBANG Niswah, Choriun; aini, Fitratul; Marlisa, Lisa
Biota Vol 2 No 2 (2016): Jurnal Biota 2016
Publisher : Faculty of Science and Technology State Islamic University Raden Fatah Palembang

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Abstract

The result comparison of Biology student by using cooperative learning type Two Stay Two Stray with Demonstration method (an experiment in Junior High Scool of Adabiyah Palembang. The purpose of this research is to find out the result of comparison between the students that learn by cooperative learning  typeTwo Stay Two Stray with Demonstration method. This research was done in junior high scool of Adabiyah Palembang in VII.1 grade which consists of 35 students. The data of learning results was taken by using the test instrument of learning result (24 item) and the observation sheets of the implemention of the t-test with 5% significant percentage. It can be seen fron the result of the posttest, t count table (2,96> 2,00). It means Ho rejected and Ha is accepted. So it can be concluded that there is a difference between the Biology learning result of student who has been taught with the cooperative learning approach type Two Stay Two Stray and the Demonstration method with the mean from learning Two Stay Two Stray are 76,00 mean while, in the Demonstration method is only 65,43 so the result of the class which uses Two Stay Two Stray learning method is better than Demonstration method.