Orryani Lambui, Orryani
Tadulako University

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UJI DAYA HAMBAT EKSTRAK DAUN LALUMPA (Melastoma malabathricum L.) TERHADAP PERTUMBUHAN BAKTERI Vibrio cholerae dan Staphylococcus aureus Mutmainnah, Mutmainnah; Harso, Wahyu; Lambui, Orryani
Biocelebes Vol. 13 No. 2 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Vibrio cholera and Staphylococcus aureus are a gram negative and a gram positive bacteria respectively. Both of them can cause diseaces in human. They have differences in their cell wall composition. Differences in both bacteria in the resistance to antibacterial compounds interesting to learn. The aim of this study was to observe inhibition of Melastoma malabathricum leave extract to the growth of V. Cholera and S. aureus. The study was conducted with Completely Randomized Design. The treatment was tested with 10%, 20%, 40%, 60%, 80% of leave extract concentration. Amoxicillin 2% and Na-CMC 1% were also treated as positif and negative control. Each treatments was repeated three times. Extract was obtained by maceration method. Extract was injected on bacterial growth medium by well diffusion method. The result showed that increasing extract concentration increased inhibition of growth to both bacteria. V. cholera tended to be more resistant than S. aureus.
UJI DAYA HAMBAT EKSTRAK DAUN BANDOTAN (Ageratum conyzoides L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus Mengkido, Melsi; Lambui, Orryani; Harso, Wahyu
Biocelebes Vol. 13 No. 2 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Ageratum conyzoides L. is commonly known as weed species however leaf from this plant is used as traditional medicine for wound and skin infection. Furthermore it is important to test A. conyzoides L. leaves extract can inhibit the growth of Staphylococcus aureus bacteria which is causing infection disease. The content of this leaf which can inhibit bacterial growth is also obeserved. The study was conducted by growing S. aureus bacteria on nutrient agar (NA) and then a number of leave extract from A. conyzoides leaves (7.5, 15.0, 35 and 50%) were injected to NA by well diffusion method. Amoxicillin 0,6% and DMSO 1% were also injected as positve control and negative control respectively. Inhibition zone was measured based on diameter formed. The results showed that increasing leaves extract increased inhibition of S. aureus growth however 50% leave extract inhibited S. aureus growth less than Amoxicilin 0,6%.A. conyzoides leaf can inhibit S. aureus growth may be caused by terpenoid, fenol, saponin and alkaloid on its content.
Uji Daya Hambat Ekstrak Daun Sirih Hutan (Piper aduncum L.) Terhadap Pertumbuhan Bakteri Streptococcus mutans Safriana, Nunung; Lambui, Orryani; Ramadanil, Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research about inhibition test of leaf extract of Piper aduncum L. to the growth of bacteria Streptococcus mutans have been conducted during periods of July to December 2016, on aim of this research was to study the inhibition test from leaf extract of P. aduncum L. the growth of the bacteria S. mutans and the amount of compound contained in the leaf of P. aduncum L. The extraction method  was used is maseration method and testing of the inhibition of the extract to the bacteria S. mutans by disc diffusion method. This research is compiled in a completely randomized designed (CRD) with 6 treatments and 3 replications. The treatments were leaf extract concentration 30%, 45%, 60%, 75%, antibiotic Tetracycline hydrochloride 5% as the positive control and negatif control aquades. The results showed that the concentrations  of leaf extract 75% produced the greatest inhibition zone is 13,1 mm. this indicates tha the leaf extract of P. aduncum L. have inhibitory better.  Phytochemical screening results showed that there were compound flavonoid, tannin, saponin and alkaloid that can inhibit the growth of bacteria.
Uji Daya Hambat Ekstrak Kulit Batang Kelor (Moringa oleifera Lamk.) Terhadap Pertumbuhan Bakteri Vibrio cholerae Labambe, Nur Afiyah; Lambui, Orryani; Ramadanil, Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The research about the inhibition test of bark extract of Moringa oleifera Lamk. to the growth of bactery Vibrio cholerae. It has been conducted on July until December. The aim of this esearch was to determine the effectiveness of bark extract of M. oleifera Lamk. to the growth of V. cholera bacteria and the content of bark extract M. oleifera Lamk. The extraction method used in this research was maceration testing method toward V. cholerae bacteria by using disc diffusion method. This research designed in a completely randomized design (CRD) with 6 treatments and 3 repetitions. At this research using stem bark extract concentration of 20%, 40%, 60% and 80%, the positive control using tetracycline hydrochloride 3% and a negative control using distilled water. The results showed that the concentration of bark extract 80% produced the greatest inhibition zone is 23.8 mm. This shows that the extract of the bark of Moringa oleifera Lamk. can inhibit Vibrio cholerae bactery.
KULTIVASI MIKROALGA ISOLAT LOKAL PADA MEDIUM EKSTRAK TAUGE Imelda, Silvia; Claudia, Cindy; Lambui, Orryani; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 2 (2018): Volume 7 Number 2 (August 2018)
Publisher : Univ. Tadulako

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Abstract

Mikroalga adalah organisme perairan yang dikenal dengan fitoplankton. Mikroalga dapat melakukan fotosintesis dan hidup dari nutrien anorganik. Penelitian ini bertujuan untuk mengetahui pertumbuhan mikroalga isolat lokal asal kolam ikan di Desa Langaleso Kecamatan Dolo, Kabupaten Sigi Biromaru pada beragam konsentrasi medium dengan penambahan ekstrak tauge dan menentukan konsentrasi ekstrak tauge yang menghasilkan kepadatan sel tertinggi. Metode yang digunakan dalam penelitian ini adalah eksperimental dengan 6 perlakuan konsentrasi yaitu ekstrak tauge 0, 4, 6, 8 dan 10%.  Hasil isolasi mikroalga diperoleh satu jenis mikroalga yaitu Chlorella. Hasil penelitian menunjukkan bahwa konsentrasi esktrak tauge mempengaruhi pertumbuhan Chlorella. Penambahan ekstrak tauge 10% mampu mendorong pertumbuhan mikrolaga Chlorella pada hari ke- 1 sampai hari ke- 3 dengan kepadatan sel tertinggi sebesar 7,4 x 108 sel/mL.
UJI DAYA HAMBAT EKSTRAK DAUN SIRIH HUTAN (Piper aduncum L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Escherichia coli Hallianah, Is Patuh; Lambui, Orryani; Ramadanil, Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Research about the inhibition test of leaf extract of Piper aduncum L.  on the growth of Staphylococcus aureus and Escherichia coli have been conducted during period of July to December 2016. The aim of this research was to study the effectiveness of leaf extract Piper aduncum L. in inhibition  of S. aureus and E. coli. The extraction method used in the study was method maceration, meanwhile bioassay of extract on the bacteria. by disc diffusion method. The research was design by Completely Randomize Design (CDR) with 5 treatments and 3 replications. The treatments were leaf extract concentration 10%, 30% and 60%.  Positive control Amoxicillin 3% and negative control aquadest. The result showed that leaf extract of Piper aduncum L. had  inhibition effect to the growth of bacterias. The extract concentrate 60% produced the biggest inhibition zone for both S. aureus and E. coli 14 mm. Phytochemical analyses has showed that leaf extract of Piper aduncum L. contained compounds flavonoids, tannins, saponins and alkaloids.UJI DAYA HAMBAT EKSTRAK DAUN SIRIH HUTAN (Piper aduncum L.) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus DAN Escherichia coli
STUDI ETNOBOTANI TUMBUHAN OBAT PADA MASYARAKAT SUKU PAMONA DI DESA BUYUMPONDOLI, KECAMATAN PAMONA PUSELEMBA, KABUPATEN POSO, SULAWESI TENGAH Bandjolu, Kurniawan P.; Lambui, Orryani; Ramadanil, Ramadanil
Biocelebes Vol. 13 No. 1 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The objectives of this study was to determine value of plants for Pamona community and to identify plant species that used traditionally as medicine. The method was survey method by using direct interview to thirteen (13) respondents. The number of respondents was determined by purposive and snow ball technique sampling. We recorded that there were thirty two (32) species of plant that used by Pamona community as medicine. Based on plant organ, the Pamona community used fruit 3 species, leaf 22 species, stem 4 species, rhizome 2 species and fruit skin 1 species.
KULTIVASI MIKROALGA ISOLAT LOKAL PADA MEDIUM SUPLEMEN AIR KELAPA Claudia, Cindy; Imelda, Silvia; Lambui, Orryani; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 3 (2018): Volume 7 Number 3 (December 2018)
Publisher : Univ. Tadulako

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Abstract

Mikroalga merupakan tumbuhan tingkat rendah yang memiliki peranan yang sangat penting dalam ekosistem akuatik sebagai produsen primer dan pensuplai oksigen perairan. Pertumbuhan mikroalga dipengaruhi oleh kondisi lingkungan dan nutrien. Penelitian ini bertujuan mengetahui pertumbuhan kultivasi mikroalga isolat lokal pada medium suplemen air kelapa dan mengetahui konsentrasi air kelapa yang paling efektif dalam meningkatkan kultivasi mikroalga  isolat lokal. Penelitian ini bersifat ekperimental dengan 5 perlakuan yaitu menggunakan media air kelapa dengan konsentrasi 0, 2,5, 5, 7,5 dan 10% dengan 3 ulangan setiap perlakuan. Penelitian ini mendapatkan hasil identifikasi mikroalga jenis Scenedesmus.  Laju pertumbuhan sel tertinggi diperoleh pada medium dengan penambahan 10% air kelapa dengan kerapatan sel yaitu 6,7% × 108 sel/mL pada hari ke-3 setelah kultur
ISOLASI DAN IDENTIFIKASI BAKTERI TANAH DI HUTAN SEKITAR DANAU KALIMPA’A, KAWASAN TAMAN NASIONAL LORE LINDU, SULAWESI TENGAH Lambui, Orryani; Jannah, Magfirahtul
Natural Science: Journal of Science and Technology Vol 6, No 1 (2017): Volume 6 Number 1 (March 2017)
Publisher : Univ. Tadulako

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Abstract

Taman Nasional Lore Lindu Sulawesi Tengah memiliki produktivitas serasah yang tinggi, sehingga sangat bergantung pada keberadaan mikroorganisme tanah untuk mendekomposisinya menjadi unsur hara yang dimanfaatkan oleh tumbuhan. Kondisi hutan primer yang masih alami dapat mendukung pertumbuhan dan keanekaragaman mikroorganisme tanah terutama bakteri. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis bakteri tanah di hutan sekitar Danau Kalimpa?a kawasan Taman Nasional Lore Lindu. Penelitian ini dilakukan pada bulan Oktober sampai Desember 2016 di Laboratorium Bioteknologi Jurusan Biologi FMIPA Universitas Tadulako. Bakteri diisolasi dari sampel tanah rizosfir yang diambil secara random di beberapa area kecil, menggunakan metode enrichment dan spread plate untuk memperoleh isolat. Isolat dimurnikan dengan metode streak plate kemudian diindentifikasi berdasarkan uji mikrobiologis dan uji biokimiawi. Dari sampel tanah hutan di sekitar Danau Kalimpa?a Kawasan Taman Nasional Lore Lindu berhasil diisolasi 5 bakteri isolat terpilih dengan kode isolat DKL 1, DKL 2, DKL 3, DKL 4 dan DKL 5. Berdasarkan hasil identifikasi secara fenotipik, isolat terpilih merupakan anggota kelompok bakteri Gram negatif dengan bentuk sel coccus dan bacil, ada yang bersifat motil dan non motil, serta mampu menggunakan karbohidrat sebagai sumber energinya melalui proses fermentasi
KARAKTERISASI SELULASE ASAL BAKTERI TANAH DANAU KALIMPA’A SULAWESI TENGAH Marina, Marina; Lambui, Orryani; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 2 (2018): Volume 7 Number 2 (August 2018)
Publisher : Univ. Tadulako

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Abstract

Selulase adalah enzim yang dapat dihasilkan oleh bakteri selulolitik, memiliki kemampuan dalam menguraikan selulosa menjadi monomer yang lebih sederhana di alam. Danau Kalimpa?a yang merupakan kawasan Taman Nasional Lore Lindu Sulawesi Tengah memiliki produktivitas seresah yang tinggi, sehingga sangat bergantung pada keberadaan mikroorganisme tanah termasuk bakteri selulolitik sebagai dekomposer. Pada penelitian ini telah dilakukan isolasi dan karakterisasi enzim endogenous. Metode yang digunakan dalam penelitian ini adalah deskriptif kualitatif dan deskripsi aktivitas enzim dipaparkan secara kuantitatif. Karakterisasi selulase ditentukan dengan menguji aktivitas enzim pada variasi suhu (30°C, 40°C, dan 50°C) dan variasi pH (5.0; 6.0; 7.0; dan 8.0). Hasil isolasi bakteri diperoleh 5 isolat terbaik (K1, K2, K3, K4 dan K5) yang memiliki kemampuan dalam mendegradasi selulosa. Secara kualitatif, isolat K4 dapat mendegradasi selulosa lebih cepat dibanding isolat lain berdasarkan pembentukan zona bening dengan Indeks Aktivitas Selulase (IAS) sebesar 3,88 mm. Secara kuantitatif, aktivitas selulase ditentukan berdasarkan kadar gula reduksi yang dihasilkan menggunakan metode DNS (Di Nitro Salisilic Acid). Isolat K2 mampu menghasilkan enzim dengan aktivitas selulase tertinggi yaitu 0,30 U/ml dan aktivitas selulase terendah dihasilkan oleh isolat K1 yaitu 0,001 U/ml. Suhu dan pH optimum selulase adalah 40°C dan pH 6, yang menghasilkan aktivitas enzim sebesr 0,279 U/ml.