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Aktivitas Larvisida Ekstrak Terpurifikasi Rimpang Zingiber zerumbet(L) Smith terhadap Larva Aedes aegypti Murini, Tri; Wahyuningsih, Mae Sri Hartati; Tunggul Satoto, Tri Baskoro; Fudholi, Achmad -
Jurnal Kedokteran Brawijaya Vol 29, No. 4 (2017)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2017.029.04.2

Abstract

Saat ini ditemukan bahwa larva nyamuk telah  resisten terhadap larvisida konvensional. Oleh karena itu, penting untuk mengembangkan larvisida baru dari sumber bahan alam seperti rimpang Z. zerumbet, pada penelitian yang sebelumnya berpotensi sebagai larvisida pada Artemia salina Leach dan Anopheles nunestovary. Aktivitas larvisida ekstrak metanol rimpang Z. zerumbet yang telah dipurifikasi belum pernah diteliti. Tujuan penelitian ini adalah untuk mengkaji aktivitas larvisida ekstrak terpurifikasi rimpang Z. zerumbet terhadap larva Ae. aegypti. Penelitian ini merupakan penelitian eksplorasi laboratories dengan rancangan post test-only control group. Penelitian ini  dilakukan pada dua ekstrak yaitu  ekstrak metanol rimpang Z. zerumbet dan ekstrak metanol rimpang Z. zerumbet yang dipurifikasi dengan petroleum eter (PE). Dua puluh lima  larva instar III-IV Ae. aegypti  dipapar dengan tujuh konsentrasi yang berbeda dari ekstrak metanol rimpang Z. zerumbet. Tingkat kematian  larva dihitung dan kemudian dianalisis dengan menggunakan regresi analisa probit untuk mendapatkan LC50. Setelah mengetahui LC50 tersebut, studi dilanjutkan dengan purifikasi ekstrak metanol Z. zerumbet menggunakan PE sebagai pelarut. Hasil ekstrak yang telah dipurifikasi baik yang larut maupun tidak larut PE diperlakukan pada  larva instar III-IV Ae.aegypti menggunakan metode yang sama seperti yang  disebutkan terdahulu. Nilai LC50 ekstrak metanol, larut PE dan tidak larut PE hasil purifikasi ekstrak metanol rimpang Z. zerumbet adalah 153,57±4,01 ppm; 67,01±2,35 ppm; dan 2287±147,91 ppm. Purifikasi ekstrak metanol rimpang Z. zerumbet  yang larut PE memiliki aktivitas larvisida yang kuat terhadap larva instar III-IV  Ae. aegypti  dan mempunyai potensi untuk dikembangkan sebagai bahan larvisida  baru.
The effect of a-terpineol on cell cycle, apoptosis and Bcl-2 family protein expression of breast cancer cell line MCF-7 Candrasari, Damiana Sapta; Mubarika, Sofia; Wahyuningsih, Mae Sri Hartati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.147 KB) | DOI: 10.19106/JMedSci004702201502

Abstract

ABSTRACT The cytotoxic activity of a-terpineol on T47D and HeLa cancer cell lines have been reported. This study was conducted to evaluate the effect of a-terpineol on cell cycle, apoptosis and Bcl-2 as well as Bax expression on MCF-7 cell line. The cytotoxic activity of a-terpineol was determined using MTT cell assay. Cell cycle and apoptosis were analysed using flowcytometry, whereas Bcl-2 and Bax expression were evaluated using immunohistochemistry. The results showed that a-terpineol had cytotoxic effect on the MCF-7 cell lines with an IC50 value of 33.0 ± 5.4 μg/mL. a-Terpineol induced cell accumulation in Sub-G1 lead to apoptosis of the MCF-7 cell. Moreover, a-terpineol inhibited Bcl-2 and induced Bax expressions. In conclusion, a-terpineol has potential anticancer activity against MCF-7 cancer cell line trough through cells cycle inhibition and apoptosis stimulation.
Antifibrotic Effect of Ethanolic Extract of Nerium indicum Mill. Standardized 5α-Oleandrin on Keloid Fibroblasts Cells Wahyuningsih, Mae Sri Hartati; Yuliani, Fara Silvia; Rahmawati, Dwiki Yuliya; Pratiwi, Annisa Nurul
Majalah Obat Tradisional Vol 23, No 1 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.022 KB) | DOI: 10.22146/mot.35116

Abstract

Keloid is a skin lesion caused by an abnormal proliferation of fibroblasts and the deposition of collagen in the wound healing process. Therapy for keloids is relatively limited and mostly has side effects. Recent studies show that 5α-oleandrin from N. indicum Mill. (N. indicum) has anti-keloid effect by inhibiting (proliferation, migration activity and TGF-β1 expression) keloid fibroblast. Further studies with N. indicum extract standardized 5α-oleandrin should be conducted to develop it as an anti-keloid agent by performing at proliferative effects, collagen accumulation, and keloid fibroblast migration. The antifibrotic activity of N. indicum extract on the proliferation of keloid fibroblasts was measured by MTT assay, whereas collagen deposits were observed by Sirius Red method. Observation of keloid fibroblast migration by scratch assay is according to Liang et al. (2009). Ethanol extract of N. indicum standardized 5α-oleandrin can inhibit the proliferation of keloid fibroblas with IC50 0.458 μg/mL, also inhibited collagen deposits with IC50 0.055 μg/mL at incubation for 72 hours. In incubation 48 hours after treatment with ethanol extract of 1.0 μg/mL occurred inhibition of migration significantly compared with control.
Pemberian jahe instan terhadap kejadian mual muntah dan asupan energi pada ibu hamil trimester pertama Setyawati, Nurfalah; Wahyuningsih, Mae Sri Hartati; Nurdiati, Detty Siti
Jurnal Gizi Klinik Indonesia Vol 10, No 4 (2014): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, Fakultas Kedokteran UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijcn.18871

Abstract

Background: Pregnancy causes increased energy metabolism, therefore needs of energy and other nutrients increase during pregnancy. Lack of energy and nutrients could adversely affect the mother and fetus. Nausea and vomiting is a common problem in the first trimester of pregnancy. If not treated the mother and fetus can be at risk of malnutrition due to lack of energy intake. Traditional treatment is an option that is considered safe. Ginger has been used to treat a variety of medical problems including nausea and vomiting. Ginger works in the digestive tract by increasing the gastric and intestinal motility.Objective: This study aimed to determine the effectiveness of instant ginger containing 290 mg ginger juice, to reduced the incidence of nausea and vomiting of pregnancy and increased maternal energy intake during the 24 hours.Method: The study design is experimental, the pre-post control group, involving 101 pregnant women with gestational age less than 15 weeks in RSB Sayang Ibu, puskesmas Klandasan Ilir and Sepinggan in Balikpapan. Research subjects were drawn at random and given the instant ginger or placebo, 2 times a day for a week. All of the subjects were assessed on the level of nausea, vomiting and a 24-hour energy intake during pre and post intervention. The use of drugs or supplementation from the doctor/midwife were recorded and not be stopped.Results: The rate of nausea and vomiting decreased significantly in the ginger group (52%) compared to placebo (17.7%) (p<0.05). Instant ginger may reduce nausea and vomiting rate 6 times higher than placebo (p<0.05). The average amount of energy intake (calories) for 24 hours increased significantly in the ginger group (1629.58 kcal ± 468.04 kcal; p<0.05) compared to placebo (1160.27 kcal ± 341.85 kcal; p>0.05). Instant ginger may increase the average amount of energy intake 24.5 times higher than placebo (p<0.05).Conclusion: Instant ginger effective to reduce nausea and vomiting of pregnancy and increase the amount of energy intake (calories) in pregnant women who experience nausea and vomiting of pregnancy.
EFEK KOMBINASI EKSTRAK Tithonia diversifolia (HEMSLEY) A.GRAY DAN Curcuma domestica VAL TERHADAP NODUL DAN BERAT BADAN TIKUS MODEL KANKER Halimah, Wahyu Nur; Pratiwi, Woro Rukmi; Wahyuningsih, Mae Sri Hartati
Majalah Farmaseutik Vol 13, No 1 (2017)
Publisher : Fakultas Farmasi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] merupakan kandidat tanaman berpotensi antikanker yang telah dibuktikan sebelumnya memiliki efek sitotoksik terhadap sel kanker WiDr, IC50=61,55 ug/mL. Kunyit (Curcuma domestica Val.) banyak digunakan dalam pengobatan tradisional, mengandung kurkuminoid yang memiliki aktivitas antikanker dengan memodulasi fungsi imun yang dimediasi oleh limfosit. Dengan adanya efek imunostimulan dari kunyit apabila dicampur dengan kembang bulan sebagai kandidat antikanker diharapkan akan bersinergis lebih baik. Tujuan penelitian ini untuk mengkaji potensi kombinasi ekstrak etanol T. diversifolia dan C. domestica pada hewan model kanker dengan cara menghitung berat badan, jumlah nodul dan volume nodul hewan coba. Penelitian ini adalah penelitian kuasi eksperimental. Kombinasi ekstrak etanol 70% daun kembang bulan dan rimpang kunyit dibuat suspensi dan dibagi menjadi 3 dosis yaitu dosis 40:150mg, 80:150mg, dan 160:150mg. Kemudian diberikan peroral pada masing-masing kelompok tikus yang telah mengalami kanker payudara setelah diinduksi dengan DMBA. Kemudian diamati berat badan, jumlah nodul, dan volume nodul tikus yang dibandingkan dengan kelompok kontrol negatif dan kelompok kontrol positif (doksorubisin). Persentase perubahan pada masing-masing kelompok perlakuan dibandingkan dan diuji secara statistik menggunakan analisis Anova. Hasil menunjukkan bahwa perbandingan berat badan tikus antara kelompok kontrol doksorubisin dan kelompok dosis 40:150mg, 80:150mg, dan 160:150mg menunjukkan tidak berbeda signifikan. Perbandingan volume nodul kanker payudara antara kelompok kontrol dan kelompok uji menunjukkan hasil yang tidak berbeda signifikan. Kombinasi ekstrak etanol  kembang bulan dan rimpang kunyit tidak mempunyai efek signifikan terhadap berat badan dan nodul kanker payudara
5α-Oleandrin reduce Bcl-2 protein and increase Bax protein expression on Hela cervical cancer cell Wahyuningsih, Mae Sri Hartati; Mubarika, Sofia; Ganjar, Ibnu G.; Wahyuono, Subagus; Takeya, Tatsuo
Universa Medicina Vol 36, No 2 (2017)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2017.v36.102-109

Abstract

BACKGROUNDThe leaves of Nerium indicum Mill. have been utilized traditionally to cure cancer. By using Bioassay guided extraction and isolation method, three compounds (NiO-1, NiO-2, NiO-3) were isolated from an active fraction of the CHCl3 extract of N. indicum leaves. NiO-2 identified as 5α- oleandrin is the best cytotoxic compound on HeLa cervical cancer cell in vitro among the other two. However mechanism of action of the compound hasn’t been evaluated yet. The aims of this study were to determine the mechanism of action at molecular level.METHODSThe action mechanism of 5α-oleandrin on HeLa cervical cancer cells was analyzed by staining the cells with Hoechst 33342; the agarose gel electrophoresis was aimed to determine the DNA fragmentation and the western blotting was aimed to determine the Bcl-2 and Bax protein expression.RESULTSIncubation of HeLa cervical cancer cell with 5α-oleandrin at the concentration 3,47x10-4 mM (24 hours) followed by staining with Hoechst 33342, a broken up light blue color of nucleus was observed (compared with intensive color of untreated control). By gel electrophoresis (at the same concentration of the tested compound), a smear band at about 200 bp was observed. In addition, cells treated with 5α-oleandrin displayed a decreasing of the Bcl-2 protein expression and increasing of the Bax protein expressionCONCLUSION5α-oleandrin induced HeLa cervical cancer cells DNA fragmentation observed by the presence of a smear band at about 200 bp indicative the apoptotic occurrence. 5α-oleandrin induces apoptosis by reducing the Bcl-2 protein expression but the Bax protein expression increases.
Effects of Centella asiatica L. Capsul on Muscle Disfunction on Elderly People Pramantara, I Dewa Putu; Kertia, Nyoman Nyoman; Wahyuningsih, Mae Sri Hartati; Arfian, Nur
Majalah Obat Tradisional Vol 23, No 2 (2018)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1765.997 KB) | DOI: 10.22146/mot.37990

Abstract

Pegagan (Centella asiatica L) has been traditionally used to treat wounds, disordered nerve system, blood circulation, and prevent muscle disorders. Extract of Pegagan was considered safe and non-toxic as the acuut and chronic toxicity tests on male SD rats were not significant. It meant that further study was potential to be proceeded. Study of C.asiatica on muscle function of the elderly has not been done, therefore this study is aimed to observe  effects of C.asiatica extract on muscle masses, holding hands, walking speed (4.57 meter), walking test (6 minutes), and Time Up and Go test of the elderly. This study is designed for clinical double blind study with 2 groups of people, these are C. asiatica (18 people) treated with capsul (550 mg C. asiatica extract/day for 90 days) and placebo (17 people). This study is performed in the RSUP Dr. Sardjito hospital at the geriatric division. Subjects for this study were healthy elderly people and fit with the criteria of subjects for experiment. Result of the study, muscle masses do not decrease and all the measurements are not different with that of placebo. Except that the walking speed (6 minutes) of the treated subjects is better than that of the placebo. The conclusion of the study, the capsul containing C. asiatica extract (550 mg, once a day for 90 days) increases the strengh of hands holding, and the walking test (6 minutes) almost twice better than that of the placebo although the muscle masses does not increase significantly.
Efek Kombinasi Ekstrak Tithonia diversifolia (Hemsley) A.Gray dan Curcuma domestica Val terhadap Nodul dan Berat Badan Tikus Model Kanker Wahyuningsih, Mae Sri Hartati; Halimah, Wahyu Nur; Prtiwi, Woro Rukmi
Majalah Farmaseutik Vol 13, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.174 KB) | DOI: 10.22146/farmaseutik.v13i1.38465

Abstract

Kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] merupakan kandidattanaman berpotensi antikanker yang telah dibuktikan sebelumnya memiliki efek sitotoksikterhadap sel kanker WiDr, IC50=61,55 ug/mL. Kunyit (Curcuma domestica Val.) banyakdigunakan dalam pengobatan tradisional, mengandung kurkuminoid yang memilikiaktivitas antikanker dengan memodulasi fungsi imun yang dimediasi oleh limfosit. Denganadanya efek imunostimulan dari kunyit apabila dicampur dengan kembang bulan sebagaikandidat antikanker diharapkan akan bersinergis lebih baik. Tujuan penelitian ini untukmengkaji potensi kombinasi ekstrak etanol T. diversifolia dan C. domestica pada hewanmodel kanker dengan cara menghitung berat badan, jumlah nodul dan volume nodul hewancoba. Penelitian ini adalah penelitian kuasi eksperimental. Kombinasi ekstrak etanol 70%daun kembang bulan dan rimpang kunyit dibuat suspensi dan dibagi menjadi 3 dosis yaitudosis 40:150mg, 80:150mg, dan 160:150mg. Kemudian diberikan peroral pada masingmasing kelompok tikus yang telah mengalami kanker payudara setelah diinduksi dengan DMBA. Kemudian diamati berat badan, jumlah nodul, dan volume nodul tikus yangdibandingkan dengan kelompok kontrol negatif dan kelompok kontrol positif(doksorubisin). Persentase perubahan pada masing-masing kelompok perlakuandibandingkan dan diuji secara statistik menggunakan analisis Anova. Hasil menunjukkanbahwa perbandingan berat badan tikus antara kelompok kontrol doksorubisin dankelompok dosis 40:150mg, 80:150mg, dan 160:150mg menunjukkan tidak berbedasignifikan. Perbandingan volume nodul kanker payudara antara kelompok kontrol dankelompok uji menunjukkan hasil yang tidak berbeda signifikan. Kombinasi ekstrak etanolkembang bulan dan rimpang kunyit tidak mempunyai efek signifikan terhadap berat badandan nodul kanker payudara.
Pemberian jahe instan terhadap kejadian mual muntah dan asupan energi pada ibu hamil trimester pertama Setyawati, Nurfalah; Wahyuningsih, Mae Sri Hartati; Nurdiati, Detty Siti
Jurnal Gizi Klinik Indonesia Vol 10, No 4 (2014): April
Publisher : Minat S2 Gizi dan Kesehatan, Prodi S2 IKM, FK-KMK UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.099 KB) | DOI: 10.22146/ijcn.18871

Abstract

Background: Pregnancy causes increased energy metabolism, therefore needs of energy and other nutrients increase during pregnancy. Lack of energy and nutrients could adversely affect the mother and fetus. Nausea and vomiting is a common problem in the first trimester of pregnancy. If not treated the mother and fetus can be at risk of malnutrition due to lack of energy intake. Traditional treatment is an option that is considered safe. Ginger has been used to treat a variety of medical problems including nausea and vomiting. Ginger works in the digestive tract by increasing the gastric and intestinal motility.Objective: This study aimed to determine the effectiveness of instant ginger containing 290 mg ginger juice, to reduced the incidence of nausea and vomiting of pregnancy and increased maternal energy intake during the 24 hours.Method: The study design is experimental, the pre-post control group, involving 101 pregnant women with gestational age less than 15 weeks in RSB Sayang Ibu, puskesmas Klandasan Ilir and Sepinggan in Balikpapan. Research subjects were drawn at random and given the instant ginger or placebo, 2 times a day for a week. All of the subjects were assessed on the level of nausea, vomiting and a 24-hour energy intake during pre and post intervention. The use of drugs or supplementation from the doctor/midwife were recorded and not be stopped.Results: The rate of nausea and vomiting decreased significantly in the ginger group (52%) compared to placebo (17.7%) (p<0.05). Instant ginger may reduce nausea and vomiting rate 6 times higher than placebo (p<0.05). The average amount of energy intake (calories) for 24 hours increased significantly in the ginger group (1629.58 kcal ± 468.04 kcal; p<0.05) compared to placebo (1160.27 kcal ± 341.85 kcal; p>0.05). Instant ginger may increase the average amount of energy intake 24.5 times higher than placebo (p<0.05).Conclusion: Instant ginger effective to reduce nausea and vomiting of pregnancy and increase the amount of energy intake (calories) in pregnant women who experience nausea and vomiting of pregnancy.
Cytotoxic activity of simvastatin in T47D breast cancer cell lines and its effect on cyclin D1 expression and apoptosis Putra, Bayu; Wahyuningsih, Mae Sri Hartati; Sholikhah, Eti Nurwening
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 49, No 2 (2017)
Publisher : Journal of the Medical Sciences (Berkala ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.91 KB) | DOI: 10.19106/JMedSci004902201701

Abstract

Background: Statins (HMG-CoA Inhibitors) is a drug used for decreasing plasma cholesterol levels and used in therapy to prevent coronary artery disease. Research in animals and epidemiological studies showed that statin therapy can decrease risk against cancer associated with cholesterol. Based on that result then research of cytotoxic activity against simvastatin knowing cultur T47D breast cancer cells and his influence in decreasing expression of cyclin D1 and induction of apoptosis has been done.Research objectives: The aims of this research is to prove activities of simvastatin against T47D breast cancer cell culture, especially to examine cytotoxic activity, cyclin D1 expression, and simvastatin effect in apoptotic induction.Research method: The type of this research is quasi experiment with using posttest with non-equivalent control group design. Cytotoxicity test performed on T47D breast cancer cell cultures using MTT assay to determine IC50 values after given simvastatine. Expression of cyclin D1 and apoptosis induction test detected using flow cytometry with antibody monoclonal anti-cyclin D1 and Annexin V-Pi, then analyzed by FACS-Calibur program.Results: Simvastatin has cytotoxic effect against T47D breast cancer cells with IC50 values 25.25 µg/mL. Simvastatin with concentrations of 6.31; 12.62; 25.25 and 50.5 µg/mL was able to decrease the cyclin D1 expression. Furthermore, simvastatin can induce apoptosis with EC50 values 26.96 µg/mL in T47D breast cancer cells.Conclusion: Simvastatin has cytotoxic activity of in T47D breast cancer cells and decreasing cyclin D1 expression and inducing apoptosis activity in T47D breast cancer cells.Keywords: simvastatin, cytotoxic, cyclin D1, apoptotic, T47D.