Andi Khaeruni R, Andi
Department of Agrotechnology, Faculty of Agriculture, Halu Oleo University

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Characterization of mannanase-producing bacteria from sago hump Wahyuni, Sri; Khaeruni R, Andi; Lianto, Lianto; Sidarmin, Sidarmin; Holilah, Holilah; Utomo, Wahyu Prasetyo; Asranudin, Asranudin
Waste Technology Vol 4, No 1 (2016)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (715.804 KB) | DOI: 10.12777/wastech.4.1.1-6

Abstract

Sago is a hardwood that can be used as source of fiber hemicellulose mannan. The abundance of mannanase waste is potential for the production of functional foods such as Manno oligosaccharides. Sago hump which is sago processing waste is very potential for growth of microorganisms because it contains lignin, cellulose, starch, minerals and vitamins that can be used as sources of carbon and energy for the growth. In this study, BLS.11-01 and BLS.11-02 isolates have been isolated and characterized from waste of sago hump. Locust bean gum was used as  substrate for measuring the activity of mannanase. Both isolates had optimum temperatures at 70°C and 60°C while they remain stable at temperature range of 30°C - 90°C. Mannanase from BLS.11-01 and BLS.11-02 had optimum pH at 6 and 7, respectively, and were also stable in wide pH range of 2-8. Co2+ was a mannanase activator for both isolates. Cu2+, Zn2+, Ni2+, Ca2+ and Fe2+ were  mannanase inhibitors for two isolates. Tween 20 (0.5% and 1.0%) and NaCl (0.5% and 1.0%) were chemical reagents that could enhance the activity of BLS.11-01 and BLS.11-02 mannanase isolates.  While tween 80 (0.5% and 1.0%), urea (0.5% and 1.0%) and SDS (0.5% and 1.0%) were chemical reagents that were not effective for both isolates mannanase activity
RESPON PERTUMBUHAN VEGETATIF TIGA VARIETAS TANAMAN JAGUNG YANG DIBERI AGENS HAYATI BIOFRESH DENGAN KOMPOSISI BAHAN ORGANIK YANG BERBEDA Musa, Musril Hakim; Aida, Nur; Khaeruni R, Andi; Wijayanto, Teguh
Berkala Penelitian Agronomi Vol 7, No 1 (2019)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/bpa.v7i1.9911

Abstract

Penelitian ini bertujuanuntuk mengetahui pengaruh perlakuan Bokashi komposisiA dan Bokashi komposisi B terhadap Karakteristik Morfologi Tiga Varietas Tanaman Jagung, dan untuk mengetahui perlakuan yang memberikan pengaruh terbaik terhadap pertumbuhan tiga varietas tanaman jagung hibrida. Penelitian ini dilaksanakan di Kebun Percobaan Balai Proteksi Tanaman PangandanHortikultura Provinsi Sulawesi Tenggara, yang berlokasi di Kecamatan Konda, Kabupaten Konawe Selatan.Penelitian ini dilakukan dengan menggunakan 2 taraf. Kedua faktor menghasilkan 9 unit petak perlakuan dan setiap perlakuan diulang sebanyak 3 kali sehingga semua perlakuan terdapat 27 unit petak  percobaan. Rancangan yang digunakan adalah Rancangan Acak Kelompok (RAK).Variabel yang diamati untuk pertumbuhan vegetatif yaitu tinggi tanaman, jumlah daun, diameter batang, panjang daun dan lebar daun.Hasil penelitian tersebut menunjukkan bahwa terdapat pertumbuhan berbeda nyata pada tanaman jagung hibrida dan salah satu perlakuan yaitu Bokashi komposisi B (B2) memberikan respon terbaik terhadap karakteristik morfologi tanaman jagung.Kata kunci : Bahan organik, Mikroba lokal, Tanaman jagung, Varietas