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PERFORMANSI PERTUMBUHAN DAN REPRODUKSI UDANG WINDU, PENAEUS MONODON YANG DIBERI PAKAN DENGAN PENAMBAHAN VITAMIN C DAN E Usman, Usman; Kamaruddin, Kamaruddin; Laining, Asda; Lante, Samuel; Tampangallo, Bunga Rante
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.14.4.2019.233-242

Abstract

Penelitian ini bertujuan mengevaluasi pengaruh penambahan vitamin C dan E dalam pakan terhadap performansi pertumbuhan dan reproduksi udang windu fase prematurasi. Dua pakan uji dengan perlakuan: penambahan vitamin C (mengandung ascorbic acid 406,1 mg/kg) dan vitamin E (mengandung 286,3 mg/kg) (PS), dan tanpa penambahan vitamin C dan E (PK). Hewan uji yang digunakan adalah udang windu fase prematurasi hasil budidaya berbobot awal 43,1±5,1 g untuk betina dan 41,9±4,4 g untuk jantan, dipelihara dalam 2 petak tambak beton berukuran 1000 m2 dengan kepadatan awal 100 ekor/tambak dan rasio udang jantan : betina adalah 4 : 6. Udang tersebut diberi pakan uji sebanyak 3% dari biomassa per hari selama 3 bulan di tambak. Selanjutnya udang dipindahkan ke bak pematangan gonad untuk mengevaluasi kinerja reproduksinya. Hasil penelitian menunjukan bahwa,laju pertumbuhan spesifik, total hemosit, jumlah sel sperma, gonada somatic indeks, dan hepatosomatic indeks cenderung lebih tinggi pada udang yang diberi pakan uji PS dibandingkan yang diberi pakan PK. Udang yang diberi pakan uji PS juga memiliki fekunditas, diameter telur, daya tetas telur, dan produksi nauplii yang cenderung lebih tinggi, meskipun secara statistik tidak berbeda nyata (P>0,05) di antara kedua perlakuan. Hasil penelitian ini menunjukkan penambahan vitamin C dan E dalam pakan cenderung meningkatkan performansi pertumbuhan dan reproduksi udang windu. This experiment was aimed to evaluate the effects of feed supplemented with vitamin C and E on the growth and reproductive performances of prematurated tiger shrimp. Two test diets were formulated as the treatments: diet with the addition of vitamin C (contain ascorbic acid of 406.1 mg/kg diet) and vitamin E (contain 286.3 mg/kg diet) (PS), and a diet without the addition of vitamin C and E (PK). The test shrimp were prematurated tiger shrimp with an average initial weight of 43.1 ± 5.1 g/ind. for females and 41.9 ± 4.4 g/ind. for males. The tiger shrimp were cultured in two of 1,000 m2 concrete ponds with a density of 100 ind./pond, and the ratio of male : female was 4 : 6. The shrimp were fed with the experimental diets at a rate of 3% of body weight/day for three months. The shrimp were then transferred to concrete tanks for reproductive evaluation. The results showed that the specific growth rates, total hemocytes, number of sperm cells, gonado-somatic index, and hepatosomatic index tended to be higher in the shrimp fed with the PS test diet compared to the shrimp fed with the PK test diet. The shrimp fed with the PS test diet had higher egg fecundity, egg diameter, hatching rate, and nauplii production compared to the shrimp fed with the PK test diet. However, the study found no significant difference (P>0.05) between the two treatments. The addition of vitamins C and E in the diet could improve the growth and reproductive performance of tiger shrimp.
PENGARUH APLIKASI DSRNA VP-15 IN VITRO DAN IN VIVO TERHADAP SINTASAN DAN RESPONS IMUN UDANG WINDU PENAEUS MONODON Parenrengi, Andi; Tenriulo, Andi; Mulyaningrum, Sri Redjeki Hesti; Lante, Samuel; Nawang, Agus
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.14.4.2019.213-223

Abstract

Teknologi RNA interference (RNAi) merupakan salah satu pendekatan yang digunakan untuk meningkatkan resistensi udang windu terhadap infeksi patogen termasuk WSSV. Pengembangan teknologi RNAi melalui aplikasi untai ganda RNA (dsRNA) yang berasal dari gen pengkode viral protein (VP) dari WSSV telah mulai dikembangkan pada udang. Penelitian ini bertujuan untuk mengkaji sintasan dan respons imun udang windu yang diberi VP-15 pasca uji tantang dengan WSSV. Udang windu (panjang 15,21 ± 1,19 cm dan bobot 32,5 ± 1,83 g) diinjeksi dengan 0,2 µg/ekor dsRNA in vitro (A), dsRNA in vivo (B), dan larutan garam/kontrol (C). Setelah tiga hari vaksinasi, udang windu ditantang dengan WSSV dengan dosis 50 µL/ekor. Pengamatan sintasan dilakukan setiap hari, sedangkan respons imun (THC dan aktivitas proPO) dilakukan pada awal dan hari ke-1, ke-3, dan ke-5 pasca uji tantang, serta analisis ekspresi gen antivirus dan histopatologi hepatopankreas dilakukan pada akhir penelitian. Hasil penelitian menunjukkan bahwa aplikasi dsRNA berpengaruh nyata (P<0,05) terhadap sintasan, THC, dan proPO. Sintasan udang windu yang diberi dsRNA VP-15 in vitro dan in vivo memberikan sintasan yang lebih tinggi 75% dibandingkan dengan kontrol. Nilai proPO tertinggi didapatkan pada dsRNA in vivo (0,138); kemudian dsRNA in vitro (0,093); dan terendah kontrol (0,061); sedangkan THC tertinggi (5.704 x 104 sel/mL) pada dsRNA in vivo, kemudian dsRNA in vitro (3.516 x 104 sel/mL) dan terendah pada perlakuan kontrol (3.322 x 104 sel/mL). Ekspresi gen antivirus semakin meningkat dengan semakin lamanya udang windu terpapar dengan WSSV. Jaringan hepatopankreas udang windu pada perlakuan kontrol (tanpa dsRNA) menunjukkan adanya kerusakan sel akibat infeksi virus.RNA interference (RNAi) technology is one of the approaches used to improve tiger shrimp Penaeus monodon resistance against WSSV infection. The development of RNAi technology through double-stranded RNA (dsRNA) isolated from gene encoding viral protein (VP) of WSSV has been applied to shrimp. This study was aimed to assess the survival rate and immune response of injected-VP-15 WSSV tiger shrimp after a challenge with WSSV. The tiger shrimp (15.21 ± 1.19 cm in length and 32.5 ± 1.83 g in weight) were injected with 0.02 µg/shrimp of in vitro dsRNA (A), in vivo dsRNA (b) and saline solution (C). After three days of vaccination, the tiger shrimp were challenged with WSSV using a dosage of 50 µL/shrimp. The survival rate was observed daily. Analyses of immune responses (hemocyte total and PO activity) were performed in several stages: before the challenge test and day-1, day-3, and day-5 post-challenge test. The expression of the antivirus gene and hepatopancreas histophatology were was observed at the end of the experiment. The results showed that the application of dsRNA significantly influenced the shrimp survival rate, THC, and proPO. Tiger shrimp injected with dsRNA VP-15 of in vitro and in vivo exhibited a higher 75% survival rate than the control (P<0.05). The highest proPO activity (0.138) was obtained at dsRNA in vivo, followed by dsRNA in vitro (0.093) and the lowest (0.061) in the control. The highest THC (5,704 x 104 cell/mL) was in vivo dsRNA, then in vitro dsRNA (3,516 x 104 cell/mL), and the lowest in the control (3,322 x 104 cell/mL). The longer the exposure with WSSV, the higher the antivirus gene expression. Histopathology analysis showed some damages to the hepatopancreas cells in the control shrimp (without dsRNA) caused by the virus infection.
PENGARUH SUMBER SPERMATOFORA PADA INSEMINASI BUATAN INDUK BETINA UDANG WINDU TURUNAN PERTAMA (F-1) TERHADAP PEMIJAHAN, KUALITAS TELUR, DAN LARVA TURUNANNYA (F-2) Lante, Samuel; Usman, Usman
Media Akuakultur Vol 14, No 2 (2019): (Desember, 2019)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.14.2.2019.63-71

Abstract

Penelitian ini bertujuan mengevaluasi pemijahan udang windu betina F-1 dan mutu telur, serta larva turunannya (F-2) dengan inseminasi buatan menggunakan sumber spermatofora udang jantan yang berbeda. Perlakuan yang dicobakan adalah induk udang windu betina F-1 diinseminasi menggunakan spermatofora dari sumber induk jantan berbeda yaitu: spermatofora induk jantan F-1 hasil budidaya (S-1), dan spermatofora induk jantan alam (S-A). Data pemijahan induk betina, kualitas telur, dan profil asam amino pada daging dan hepatopankreas jantan, serta morfologi larva dianalisis secara deskriptif, sedangkan data uji vitalitas larva dianalisis uji t. Hasil penelitian menunjukkan bahwa induk udang windu betina F-1 yang diinseminasi dengan perlakuan S-1 memiliki tingkat pemijahan 67%; fekunditas 179.257 butir/induk; total produksi telur 1.434.053 butir; tingkat pembuahan telur 86,2%; daya tetas telur 59,8%; dan total produksi nauplii 738.439 ekor yang lebih rendah dibandingkan pada induk udang F-1 yang diinseminasi perlakuan S-A yang memiliki tingkat pemijahan 75%; fekunditas 215.489 butir/induk; total produksi telur 1.939.399 butir; tingkat pembuahan telur 88,9%; daya tetas telur 62,7%; dan total produksi nauplii 1.081.140 ekor. Sementara diameter telur (248-255 µm) dan mutu larva relatif sama di antara kedua perlakuan. Profil asam amino hepatopankreas dan daging pada induk udang jantan alam lebih tinggi dibandingkan pada induk udang jantan F-1. Penggunaaan spematofora jantan alam masih lebih baik daripada jantan budidaya pada inseminasi induk betina F-1 udang windu.This study was aimed at evaluating the spawning rate of F-1 female tiger shrimp and the quality of their egg and larvae derivatives (F-2) by artificial insemination using different sources of male shrimp spermatophore. The treatments consisted of broodstock of F-1 female tiger shrimp inseminated with different male spermatophores, namely: cultivated F-1 male spermatophore (S-1), and wild male spermatophore (S-A). Data on spawning performance of F-1 female tiger shrimp and amino acid profile in the hepatopancreas and muscle of male tiger shrimp and larval morphology were analyzed descriptively.The vitality of larvae was analyzed using t-test. The results showed that the broodstock of F-1 female tiger shrimp inseminated with S-1 treatment had spawning rate of 67%; fecundity of 179,257 egg; total egg production of 1,434,053 eggs; egg fertilization rate of 86.2%; hatching rate of 59.8%; and total nauplii production of 738,439 ind. Broodstock of F-1 female inseminated with S-A treatment had higher values for spawning rate of 75%, fecundity of 215,489 egg; total egg production of 1,939,399 egg; egg fertilization rate of 88.9%; hatching rate of 62.7%; and total nauplii production of 1,081,140 ind. The produced egg diameter (248-255 ¼m) and larva quality were relatively same between the two treatments. Amino acid profiles in the hepatopancreas and muscle were higher in the wild male broodstock compared to the cultivated (F-1) male broodstock. In conclusion, wild male spermatophore is generally better than the cultivated F-1 male spermatophore for artificial insemination of female broodstock F-1. 
Pengaruh Kadar Protein Pakan terhadap Pertumbuhan dan Sintasan Udang Windu, Penaeus monodon Fab.Transveksi Lante, Samuel; Usman, Usman; Laining, Asda
Jurnal Perikanan Universitas Gadjah Mada Vol 17, No 1 (2015)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9936

Abstract

Udang windu transveksi merupakan udang yang menggunakan teknologi transgenetik dimana suatu teknologi rekayasa gen dengan mengintroduksikan satu atau lebih Deoxyribo Nucleic Acid (DNA) asing ke dalam tubuh udang dengan maksud memanipulasi genotipenya ke arah yang lebih baik dan selanjutnya di transmisikan ke keturunannya. Aplikasi transfeksi pada udang diharapkan dapat memperbaiki karakterkarakter yang berguna bagi akuakultur seperti peningkatan laju pertumbuhan dan daya tahan tubuh udang terhadap penyakit. Namun demikian, domestikasi udang windu transveksi dengan menggunakan pakan komersial selama ini memberikan pertumbuhan udang yang relatif lambat. Pertumbuhan udang yangrelatif lambat diduga salah satu penyebabnya adalah kebutuhan protein dalam pakan yang belum optimal. Penelitian ini bertujuan untuk mendapatkan kadar protein yang baik untuk mendukung pertumbuhan dan sintasan udang windu transveksi. Perlakuan yang diterapkan adalah kadar protein yang berbeda yaitu: 30%, 40%, dan 50% protein dalam pakan. Penelitian didesain dengan menggunakan rancangan acak kelompok yang terdiri dari 3 perlakuan dan 2 ulangan. Hasil penelitian menunjukkan bahwa kadar 40% protein dalam pakan memberikan efi siensi protein tertinggi dan berbeda nyata (P<0,05) dengan kadar 30% protein dalam pakan, namun keduanya tidak berbeda nyata (P>0,05) dengan kadar 50% proteindalam pakan. Pada kadar 30% dan 40% protein dalam pakan menghasilkan sintasan yang tidak berbeda nyata (P>0,05), namun keduanya berbeda nyata (P<0,05) dengan sintasan udang windu yang diberi 50% protein dalam pakan. Laju pertumbuhan spesifi k udang pada ketiga perlakuan tidak menunjukkan perbedaan yang nyata (P>0,05). Hasil penelitian ini mengindikasikan bahwa dengan kadar 40% protein dalam pakan menghasilkan efi siensi protein dan sintasan yang baik pada udang windu transveksi selama 81 hari pemeliharaan dalam bak resirkulasi.Udang windu transveksi merupakan udang yang menggunakan teknologi transgenetik dimana suatuteknologi rekayasa gen dengan mengintroduksikan satu atau lebih Deoxyribo Nucleic Acid (DNA) asing kedalam tubuh udang dengan maksud memanipulasi genotipenya ke arah yang lebih baik dan selanjutnyadi transmisikan ke keturunannya. Aplikasi transfeksi pada udang diharapkan dapat memperbaiki karakterkarakteryang berguna bagi akuakultur seperti peningkatan laju pertumbuhan dan daya tahan tubuh udangterhadap penyakit. Namun demikian, domestikasi udang windu transveksi dengan menggunakan pakankomersial selama ini memberikan pertumbuhan udang yang relatif lambat. Pertumbuhan udang yangrelatif lambat diduga salah satu penyebabnya adalah kebutuhan protein dalam pakan yang belum optimal.Penelitian ini bertujuan untuk mendapatkan kadar protein yang baik untuk mendukung pertumbuhan dansintasan udang windu transveksi. Perlakuan yang diterapkan adalah kadar protein yang berbeda yaitu:30%, 40%, dan 50% protein dalam pakan. Penelitian didesain dengan menggunakan rancangan acakkelompok yang terdiri dari 3 perlakuan dan 2 ulangan. Hasil penelitian menunjukkan bahwa kadar 40%protein dalam pakan memberikan efi siensi protein tertinggi dan berbeda nyata (P<0,05) dengan kadar30% protein dalam pakan, namun keduanya tidak berbeda nyata (P>0,05) dengan kadar 50% proteindalam pakan. Pada kadar 30% dan 40% protein dalam pakan menghasilkan sintasan yang tidak berbedanyata (P>0,05), namun keduanya berbeda nyata (P<0,05) dengan sintasan udang windu yang diberi50% protein dalam pakan. Laju pertumbuhan spesifi k udang pada ketiga perlakuan tidak menunjukkanperbedaan yang nyata (P>0,05). Hasil penelitian ini mengindikasikan bahwa dengan kadar 40% proteindalam pakan menghasilkan efi siensi protein dan sintasan yang baik pada udang windu transveksi selama81 hari pemeliharaan dalam bak resirkulasi.
PENINGKATAN PERFORMA REPRODUKSI INDUK UDANG WINDU, Penaeus monodon JANTAN TAMBAK MELALUI APLIKASI BAHAN ADITIF DALAM PAKAN MATURASI Laining, Asda; Lante, Samuel; Kamaruddin, Kamaruddin
Jurnal Perikanan Universitas Gadjah Mada Vol 16, No 2 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9106

Abstract

Low mating rate in domesticated tiger shrimp both in pond and controlled tank is probably due to immature spermatozoa because of defi ciency of several micronutrients such as amino acid, certain vitamins and minerals. The purpose of this experiment was to determine the effect of commercial feed additive and to obtain its optimum dose in improving the sperm quality of pond-reared male black tiger. Test diet containing three different levels of feed additive used as treatment at 0 (SX0), 20 (SX20) dan 30 g/kg pakan (SX30). Formulation used in this experiment was standard formulation developed through previous experiment. Two hundreds and fi fty male tiger shrimps were obtained from traditional earthenpond  with weight range from 57 to 99 g and months of age around 8 months (prematuration stage). In order to evaluate the effect of dietary treatments on natural mating rate and fertiled eggs, after 4 months feeding trial, male stocks were mated with wild female tiger shrimp then observed their performances. After 2 moths feeding trial (age of male around 10 months), from 15 male stocks  visually observed, it was still found undevelope spermatophore in each of the tank. Slight development of spermatophore was observed in male stocks fed SX30 around 67% and 53% was found in shrimp fed SX0 and SX20. Furthermore, spermatophore with white color ranged from 13-20% in all dietary treatments. Number of sperm cell in male fed with SX20 was the highest 24 x106 cell and the lowest was found in male fed SX0 yaitu 75 x106 cell. Female maturing without spermatophore in its thelicum was not found in group fed SX0 and female maturing bearing spermatophore was occured in 4 females. Egg fecundity were relative similar in the two groups which was 164,166 eggs obtained in SX0 and 186,489 eggs in SX30. Even though fecundity of the two groups was quite similar, no fertiled egg was spawned by female fed SX0 while in group SX30, 34% of the eggs was fertile and hacthed around 33%. The application of additives as much as 30 g / kg in male tiger shrimp broodstock feed ponds can increase the level of fertilization of eggs by 34% higher than 0% in the feed additive.
REPLACEMENT OF FISH MEAL WITH POULTRY OFFAL MEAT IN DIETS FOR HUMPBACK GROUPER Cromileptes altivelis GROW-OUT Usman, Usman; Rachmansyah, Rachmansyah; Lante, Samuel; Kamaruddin, Kamaruddin; Ahmad, Taufik
Indonesian Aquaculture Journal Vol 1, No 1 (2006): (June 2006)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.057 KB) | DOI: 10.15578/iaj.1.1.2006.45-52

Abstract

Fish meal (FM) has traditionally ben a major ...........
EXPRESSION OF ANTIVIRAL GENE ON TIGER SHRIMP Penaeus monodon AT DIFFERENT TISSUE AND BODY SIZE Parenrengi, Andi; Tenriulo, Andi; Lante, Samuel
Indonesian Aquaculture Journal Vol 7, No 2 (2012): (December 2012)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.161 KB) | DOI: 10.15578/iaj.7.2.2012.95-104

Abstract

The role of tiger shrimp defense against invading pathogen on molecular level such antiviral gene expression is limited to be reported. Gene expression is a process which codes information of genes that is converted to the protein as a phenotype. Distribution of PmAV antivirus gene, that has been reported as an important gene on non-specific response immune, is needed to be observed to several organs/tissues and size of tiger shrimp. The aim of this study is to determine the distribution of gene antiviral expression at several organ/tissue and size of shrimp. The organs/tissues observed in this study were: gill, hepatopancres, muscle tissue, eyes, heart, stomach, gonad, and intestine. While the size of shrimp consisted of three groups, those are: (A) 10-20 g/ind., (B) 30-40 g/ind., and (C) 60-70 g/ind. Analysis of antiviral gene expression was performed by RNA extraction, followed by the cDNA syntesis, and amplification of gene expression by semi-quantitative PCR. The result of PCR optimation showed the optimal concentration of cDNA and primer was 1 μL and 50 mol, respectively for PCR final volume of 25 μL. Antiviral gene was expressed on the hepatopancreas and stomach in percentage of 50.0% and 16.7%, respectively. While the highest percentage of individual expressing the antiviral gene was observed in the shrimp size of C (66.7%), followed by B (50.0%) and A (16.7%). The result of study implied that the hepatopancreas has importantly involed in tiger shrimp defense mechanism on viral infection.
OPTIMALISASI PADAT TEBAR BENIH UDANG PAMA (Penaeus semisulcatus) PADA PENTOKOLAN DENGAN SISTEM HAPA DI TAMBAK Mangampa, Markus; Sulaeman, Sulaeman; Parenrengi, Andi; Lante, Samuel
Jurnal Riset Akuakultur Vol 3, No 2 (2008): (Agustus 2008)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (781.447 KB) | DOI: 10.15578/jra.3.2.2008.175-181

Abstract

Udang pama mempunyai nilai ekonomis yang cukup tinggi baik di pasar domestik maupun di pasar dunia. Penelitian ini bertujuan untuk menentukan kepadatan benih yang sesuai pada pentokolan yang ditokolkan di tambak dengan menggunakan hapa. Riset ini dilaksanakan pada petakan tambak 250 m2 di Instalasi Riset Perikanan Air Payau Marana, menggunakan 9 unit hapa dari waring halus berukuran 1 m x 1 m x 1 m. Benih udang pama stadia PL-12 berukuran panjang rata rata 0,11 cm/ekor dengan bobot rata-rata 0,003 g/ekor ditebar dengan kepadatan berbeda sebagai perlakuan yaitu: (A) 1.000 ekor/m2, (B) 1.500 ekor/m2, dan (C) 2.000 ekor/m2 selama 35 hari. Untuk suplai oksigen digunakan 1 aerator Hiblow dengan 2 batu aerasi/hapa. Tinggi air dalam hapa 0,80 m dan pergantian air dilakukan setelah 15 hari pemeliharaan sebanyak 30% setiap 3 hari. Diberikan pakan komersil dalam bentuk crumble dengan dosis 50%—200% dari bobot biomassa. Hasil riset menunjukkan bahwa sintasan tertinggi pada kepadatan (A) 1.000 ekor/m2 (70,83%) berbeda nyata pada kepadatan (B) 1.500 ekor/m2 (47,71%), dan (C) 2.000 ekor/m2 dengan sintasan yang sangat rendah (34,58%). Pertumbuhan tidak menunjukkan perbedaan masing-masing sebesar: (A) 3,43; (B) 3,37; dan (C) 3,42 cm/ekor. Hal yang sama ditunjukkan pada pertumbuhan bobot masing-masing perlakuan yaitu: (A) 0,348; (B) 0,286; dan (C) 0,300 g/ekor.ABSTRACT:    Differences of stocking densities of green tiger shrimp fry (Penaeus semisulcatus) reared in nursery with net-cage system. By: Markus Mangampa, Sulaeman, Andi Parenrengi, and Samuel LanteGreen tiger shrimp is an economically valuable species both in domestic and international markets. The experiment was conducted at Marana Research Station in Maros Regency using nine of one-cubic-meter net-cages submerged in a 250 m2-pond. The aim of the experiment was to investigate the optimum stocking density of green tiger shrimp post larvae during nursery with net cage system in pond. PL-12 of green tiger shrimp fry with average individual length and body weight of 0.11 cm and 0.003 g respectively were stocked at different densities i.e. A= 1,000 ind./cage; B= 1,500 ind./cage; and C= 2,000 ind./cage. Each treatment was made in triplicate and reared for 35 days. Two aeration lines were used for each cage for oxygen supply using portable aerator. Water exchange was done 15 days after stocking date at a rate of 30% of water volume in pond everyday for three days. An average water depth in pond was maintained at 0.8 m. Crumbled commercial shrimp diet was applied daily at a rate of 50%—200% of body weight (BW). Result of the experiment showed that the highest survival rate (SR) was achieved by treatment A (70.83%) which was statistically different (P&lt;0.05) to the treatment B (47.71%) and C (34.58%). Absolute length and weight growth rate were not significantly different on all treatments (P&gt;0.05).
THE EFFECT OF PHYTOECDYSTEROID OF Cycas revolua, Portulaca oleracea, AND Morus sp. ON MOLTING PERIOD, GROWTH AND SURVIVAL RATE OF TIGER SHRIMP, Penaeus monodon Rosmiati, Rosmiati; Lante, Samuel; Suryati, Emma
Indonesian Aquaculture Journal Vol 11, No 2 (2016): (December, 2016)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (465.612 KB) | DOI: 10.15578/iaj.11.2.2016.69-74

Abstract

The problem which has still been faced in the Artificial insemination (AI) is the slow of shrimp to molt. Ecdysteroid hormone has been reported to stimulate molting of tiger shrimp. This study aims to isolate ecdysteroid hormone from Cycas revoluta, Portulaca oleracea and Morus sp. and evaluate its effect on molting period, growth and survival rate of tiger shrimp. Isolation of ecdysteroid from the leaves of three species was carried out by maceration and solvent partition method. Purification of ecdysteroid used repeated column chromatography and preparative thin layer chromatography (TLC). Evaluation of the isolated phytoecdysteroid hormone effect on molting period, growth and survival rate of shrimp was done by injecting of 100 µL phytoecdyasterod (27.5 µg/shrimp) at the first somite of ventral abdomen. As the comparison, the commercial ecdysteroid (positive control) and sterile saline solution (negative control) were also injected at the concentration of 8.6 µg/shrimp and 0 µg/shrimp, respectively. Finding showed that the highest percentage of phytoecdysteroid was obtained in Portulaca oleracea, followed by Morus sp. and Cycas revoluta with the ecdysteroid content of 0.43%, 0.22%, and 0.09%, respectively. Pytoecdysteroid isolated from the three plants was able to shorten molting period of shrimp into 4, 4, 2, and 5 days earlier for Portulaca oleracea, Morus sp., Cycas revoluta, and positive control, respectively, compared to the negative control. The highest survival rate and growth were obtained at the treatment of Portulaca oleracea, followed by Morus sp. and Cycas revoluta with the survival rate, length and weight increase of 86%, 75%, and 25%, 4.42%, 2.26% and 2.16%, and 15.90%, 10.55%, and 8.73%, respectively. 
PERFORMA REPRODUKSI UDANG WINDU, Penaeus monodon TRANSGENIK PASCA INSEMINASI BUATAN MENGGUNAKAN SUMBER SPERMATOFOR YANG BERBEDA Lante, Samuel; Tenriulo, Andi; Parenrengi, Andi
Jurnal Riset Akuakultur Vol 13, No 1 (2018): (Maret 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (478.356 KB) | DOI: 10.15578/jra.13.1.2018.11-20

Abstract

Udang windu transgenik merupakan udang hasil rekayasa dengan mengintroduksikan gen antivirus yang diisolasi dari udang windu untuk menghasilkan fenotipe yang lebih baik. Domestikasi udang transgenik telah dilakukan dan berhasil memijah/bertelur, tetapi umumnya telurnya infertil yang disebabkan tidak terjadinya pembuahan di tambak pemeliharaan. Udang betina tidak kawin ditandai tidak membawa spermatofor di telikumnya. Upaya untuk mendapatkan telur fertil udang dengan inseminasi buatan (IB) perlu dilakukan. Tujuan penelitian untuk mengevaluasi performa reproduksi udang betina transgenik dan mutu larva yang dihasilkan pasca IB menggunakan sumber spermatofor yang berbeda. Penelitian ini dirancang dengan tiga perlakuan yaitu: IB menggunakan spermatofor udang windu jantan transgenik (SJT), spermatofor udang windu jantan alam Sulawesi Selatan (SulSel) (SJS) dan spermatofor udang windu jantan alam Aceh (SJA). IB dilakukan pada udang windu betina transgenik setelah dua hari moulting. Hasil penelitian menunjukkan bahwa udang windu betina transgenik pasca IB perlakuan SJT menghasilkan total telur fertil sebanyak 766.949 butir, perlakuan SJS 535.644 butir dan perlakuan SJA 678.016 butir dengan daya tetas telur fertil yaitu: pada SJT, SJS, dan SJA masing-masing adalah 53,5%; 53,7%; dan 55,0%. Uji vitalitas larva dengan perendaman dalam larutan formalin 150-200 mg/L, perendaman air tawar: 5-15 menit, dan pengeringan 3-9 menit menghasilkan sintasan larva udang yang relatif sama pada ketiga perlakuan. Nilai morfologi larva perlakuan SJT, SJA, dan SJS adalah masing-masing 85,0; 84,5; dan 75,0. Dari hasil penelitian ini mengindikasikan bahwa performa reproduksi udang windu betina transgenik dan mutu larva yang dihasilkan pasca IB tidak dipengaruhi oleh sumber spermatofor induk udang windu jantan Penaeus monodon.Transgenic tiger shrimp, Penaeus monodon has been developed in the last decade to equip shrimp with immunity against viral diseases. However, the effort to produce large quantities of specific pathogen resistance (SPR) tiger shrimp seed is hampered by several constraints in the domestication process. The successfulness of domesticated broodstock in producing larvae is very low due to low fertilization rate. An artificial insemination (AI) offers a solution to increase fertilization rate in crustacean. This study was aimed to evaluate the reproductive performance of female transgenic tiger shrimp broodstock and their larval quality after artificially inseminated with males from different sources. The spermatophores of male from different sources i.e. transgenic male spermatophore (SJT), wild male from South Sulawesi (SJS), and wild male from Aceh (SJA) were collected through electric shock and inseminated to female transgenic broodstock two days after moulting. The results showed that the total numbers of fertile eggs produced from SJT, SJS, and SJA treatment were 766,949 pcs; 535,644 pcs; and 678,016 pcs, respectively and not significantly different (P&gt;0.05). Similar to the number of fertile eggs, the hatching rate of eggs of SJT (53.5%), SJS (53.7%), and SJA (55.0%) also did not indicate any significant differences (P&gt;0.05). On the larval vitality test by soaking the larvae in formalin and freshwater as well as by air exposure at different duration showed no significant difference on the survival rate (P&gt;0.05) as indicated by score value at each treatment of 85.0, 84.5, and 75.0 for SJT, SJS, and SJA, respectively. In conclusion, the reproductive performance of female transgenic tiger shrimp and their larval quality were not affected by the different sources of spermatophores inseminated artificially during the spawning cycle.