Ibnu G. Gandjar, Ibnu G.
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta

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Cytotoxicity of oleandrin isolated from the leaves of Nerium indicum Mill. on several human cancer cell lines Wahyuningsih, Mae S.H.; Mubarika, Sofia; Bolhuis, R.L.M.; Nooter, K.; Gandjar, Ibnu G.; Wahyuono, Subagus
INDONESIAN JOURNAL OF PHARMACY Vol 15 No 2, 2004
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (335.917 KB) | DOI: 10.14499/indonesianjpharm0iss0pp96-103

Abstract

Finding anticancer drugs from natural resources still proceeds. Oleandrin isolated from Nerium indicum Mill. inhibited the growth of mieloma cell line in vitro better than that of vincristine sulphate. This study was aimed to determine the cytotoxic effect of oleandrin on various human cancer cell lines. Cytotoxic test of oleandrin on seven human cancer cell lines was done by SRB-method. The analysis was conducted by comparing the ID50 of oleandrin with that of doxorubicin and cisplatin as positive controls. This result indicated that oleandrin possessed the best cytotoxic effect on breast cancer (MCF7) with ID50 at 8.85 nM. Keywords : Oleandrin, cytotoxicity, human cancer cells, ID50
Structure identification of potential compound as selective renal anticancer isolated from Nerium indicum Mill. Leaves Wahyuningsih, Mae S.H.; Mubarika, Sofia; Hamann, Mark T.; Gandjar, Ibnu G.; Wahyuono, Subagus
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 2, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (850.839 KB) | DOI: 10.14499/indonesianjpharm0iss0pp57-64

Abstract

Oleandrin is one of cardenolida compound isolated from an active fraction of Nerium indicum Mill leaves. (fam. Apocynaceae), which have cytotoxic effect on several human cancer cells, and also to normal cells in vitro. Another compound which was potential as renal anticancer has also been isolated from N. indicum. However, its chemical structure has not been discovered. The aim of this study was to identify the potential compound as selective to renal cancer present in the leaves of N. indicum.The potential compound was isolated from the active fraction using Preparative TLC and structure elucidation was done by using spectroscopic methods (UV, IR, MS and NMR).Base on this spectra and by comparison with oleandrin data, it was indicated that the potential compound as Renal anticancer was as 16,17-dehydrodeacetyl-5a-oleandrin.Key word: N.indicum, oleandrin, cytotoxic, renal anticancer, spectroscopic
Detection of apoptosis mechanism on renal cancer cell treated by 16,17-dehydrodeacetyl-5α-oleandrin compound isolated from Nerium indicum Mill. Leaves. Wahyuningnsih, Mae S.H.; Mubarika, Sofia; Gandjar, Ibnu G.; Wahyuono, Subagus; Boersma, AWM.; Nooter, K.
INDONESIAN JOURNAL OF PHARMACY Vol 19 No 4, 2008
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.149 KB) | DOI: 10.14499/indonesianjpharm0iss0pp178-184

Abstract

The 16,17-dehydrodeacetyl-5α-oleandrin was isolated from an active fraction of Nerium indicum Mill leaves (fam. Apocynaceae). This compound was cytotoxic against various cancer cells, and selective on A498 cells (Renal cancer). However, the apoptosis mechanism was still unknown yet. Therefore, the aim of this study was to know the apoptotic mechanism of 16,17-dehydrodeacetyl-5α-oleandrin on A498 cells by FITC labeled annexin V and immunucytochemical assays. The detection of apoptotic mechanism on A498 cells was performed with FITC-conjugated annexin V using Flow Cytometry. The p53 protein expression were detected using immunocytochemical. Treatment with 16,17-hydrodeacetyl-5α-oleandrin (3.88 x 10-4 mM) using FITC-annexin V increased the percentage of the dead cells in the 24th and 48th hours incubation period. The 16,17-dehydrodeacetyl-5α-oleandrin (1,94x10-4 and 3,88x10-4 mM) raised significanly p53 protein expression (p<0,05). The percentage of the p53 protein expression increased throughout the time of samples incubation.Key words: 16,17-dehydrodeacetyl-5α-oleandrin,fluorescein isothiocyanate-annexin V, immunocytochemically, A498 cells.