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Rapid Assessment of Diverse Trichodermal Isolates of Indonesian Origin for Cellulase Production Fahrurrozi, Fahrurrozi; Ratnakomala, Shanti; Anindyawati, Trisanti; Lisdayanti, Puspita; Sukara, Endang
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/36

Abstract

Trichoderma is a well -known candidate to be promoted as cellulase producer  for the hidrolysis of lignocelluloses that contain  in  woody  biomass. The  number of trichodermal  isolates  in our  laboratory collected  from diverse ecosystem types in Indonesia increases significantly during the last 5 years. It is our aim to assess the cultures for its ability in producing cellulase. Sixty-six trichodermal isolates used in this experiment are obtained from Biotechnology Culture Collection (BTCC), Research Center for Biotechnology, Indonesian Institute of Sciences(LIPI)  The 31 isolates were isolated from District of Liwa (South Sumatra, Indonesia) and the 35 isolates from District of Maros (South Sulawesi, Indonesia). The  isolates were screened qualitatively,  7  isolates  from  Liwa and 12  isolates from Maros showed cellulolytic activity. From the results of quantitative test, two strains  (ID08-T004 and ID08-T63) showed the higher cellulolytic activity among the selected strains, 133.5 and 133.5 U/ml, respectively.  These  extracellular  enzymes  were  characterized  their  temperature  and  pH  optimum.  The temperature  optimum  for  both  enzymes  was  the  same,  50C,  with  activity  213.6  U/ml  for  enzyme  extracted from ID08-T004 and 197.3 U/ml for enzyme from ID08-T0063. The pH optimum was pH 5 of ID08-T004 with activity137.7 U/ml and pH 6 for ID08-T063 with activity 75.0 U/ml. The enzymes from ID08-T004 and ID08-T063 were stable in their temperature and pH optimal condition even after 90 minutes incubation with activity 179.0 U/ml and 86.7 U/ml, respectively. The enzyme stability was approximately 150 minutes for both enzymes in the temperature and pH optimum.   Key Words: Trichoderma, cellulase, Culture Collection
POTENSI SELULASE DALAM MENDEGRADASI LIGNOSELULOSA LIMBAH PERTANIAN UNTUK PUPUK ORGANIK Anindyawati, Trisanti
JURNAL SELULOSA Vol 45, No 02 (2010): BERITA SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.401 KB)

Abstract

It is quite evident that agriculture product waste is abundant. Further process of it will produce a value-added product such as organic fertilizer. Lignocellulose waste contain important compounds, i.e cellulose, hemicellulose and lignin (include rice straw, wood, bagasse). In the degradation process maximum results will be attained through a necessary pretreatment - mechanical, physico-chemical, chemical and biological. Lignocellulolitic microbes consisting of molds, bacteria and actinomycetes were able to degrade lignocellulosic materials to produce organic fertilizers, whereas anaerobic bacteria can produce multi-enzyme complex / cellulosome. Key words : cellulase, agricultural wastes, lignocellulose, organic fertilizer INTISARILimbah pertanian yang berlimpah merupakan suatu bahan yang mempunyai nilai tambah bila diproses lebih lanjut, salah satunya adalah untuk pupuk organik. Limbah lignoselulosa seperti jerami padi, kayu, bagas terdiri dari selulosa, hemiselulosa dan lignin. Untuk memperoleh hasil yang maksimal pada proses degradasi, diperlukan perlakuan awal yang bisa dilakukan secara mekanik, fisika-kimia, kimia dan biologi. Mikroba lignoselulolitik yang terdiri dari kapang, bakteri dan aktinomisetes dapat mendegradasi bahan lignoselulosa untuk menghasilkan pupuk organik, termasuk bakteri anaerob yang dapat menghasilkan multi enzim kompleks/selulosom.Kata kunci : selulase, limbah pertanian, lignoselulosa, pupuk organik
KONVERSI SELULOSA TANDAN KOSONG SAWIT (TKS) MENJADI ETANOL Sarwono, Rakhman; Triwahyuni, Eka; Aristiawan, Yosi; Kurniawan, Hendris Hendarsyah; Anindyawati, Trisanti
JURNAL SELULOSA Vol 4, No 01 (2014): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (517.307 KB) | DOI: 10.25269/jsel.v4i01.50

Abstract

A serious global energy crisis is thought to be originated from the imbalance rapid consumption and the non-renewable nature of the fossil fuels. A potential, yet promising route for diminising this problem might involve rapid conversion of organic waste and biomass into fuels as an alternative. Oil-palm empty fruit bunch (EFB) is the waste from the oil palm plantation which abundant amount of lignocellulosic EFB biomass. EFB biomass was used as raw material of the second generation of bioethanol production. EFB was converted into ethanol through enzymatic hydrolysis and fermentation simultaneously. Cellulose waste was then turned into glucose by enzymatic saccharification and finally fermented into ethanol. The experiment of 20 liter broth resulted in ethanol concentration of about 7.93% (w/w). Conversion of cellulose into glucose was about 60.02%, and conversion of glucose into ethanol was about 88.44%. Following distillation, ethanol of 1970 mL was obtained at a concentration of 63% (v/v).Keywords: EFB, saccharification, fermentation, glucose, ethanol  ABSTRAKAdanya krisis energi minyak bumi secara global disebabkan oleh ketimpangan antara konsumsi dan produksi minyak bumi. Guna mengimbangi ketimpangan tersebut, maka dilakukan konversi limbah organik dan biomassa menjadi bahan bakar secara tepat dan cepat. Tandan Kosong Sawit (TKS) merupakan limbah dari perkebunan sawit yang melimpah jumlahnya. Penelitian etanol generasi kedua berbahan baku biomassa lignoselulosa dilakukan melalui proses sakarifikasi selulosa menjadi glukosa secara enzimatis dan fermentasi glukosa menjadi etanol. Berdasarkan hasil yang diperoleh dari 20 liter hidrolisat didapat konsentrasi etanol sebesar 7,93% (b/b). Hasil konversi selulosa menjadi glukosa sebesar 60,02%, sedangkan konversi glukosa menjadi etanol sebesar 88,44%. Setelah dilakukan distilasi didapatkan etanol sebanyak 1970 mL dengan konsentrasi 63% (v/v).Kata kunci: TKS, sakarifikasi, fermentasi, glukosa, etanol
PROSPEK ENZIM DAN LIMBAH LIGNOSELULOSA UNTUK PRODUKSI BIOETANOL Anindyawati, Trisanti
JURNAL SELULOSA Vol 44, No 01 (2009): BERITA SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4599.154 KB) | DOI: 10.25269/jsel.v44i01.149

Abstract

Substitution bioethanol as one of energy source has been selected as an alternative source for the fossil fuel substitution. The arricultural and industrial waste can be used for the production of bioethanol. The main component in those waste materials is lignocelluloses that contained cellulose, hemicelluloses and lignin. Lignocellulose will be the main source for bioethanol production in the long term. Several enzymes have been recorded in degrading lignollulose wich are cellulolytic, hemicellulolytic as well as lignolytic. The main enzyme which has the most important role in bioethanol production are complex enzyme which degrade lignocelluloses. Bioethanol prodaction is much affected by raw materials composition, type of microorgranisms, and the fermentation condition used.Key words: lignocelluloses, cellulase, hemicellulase, lignin degrading enzyme, bioethanolINTISARI Bioetanol merupakan salah satu energy alternative pengganti minyak bumi. Bahan limbah pertanian dan industry dapat digunakan untuk produksi bioetanol. Komponen utama pada limbah pertanian dan industri yang digunakan untuk produksi bioetanol adalah lignoselulosa yang terdiri dari selulosa, hemiselulosa dan lignin. Lignoselulosa merupkan bahan utama produksi bioetanol untuk jangka panjang. Enzim yang berperan dalam degradasi lignoselulosa adalah enzim yang bersifat selulotik, hemiselulolitik dan lignolitik. Enzim utama yang berperan penting pada produksi bioetanol merupakan enzim kompleks yang mampu mendegradasi lignoselulosa. Produksi bioetanol sangat di pengaruhi oleh komposisi bahan baku, jenis mikroorganisma dan kondisi fermentasi yang digunakan.Kata Kunci : lignoselulosa, selulase, hemiselulase, enzim pendegradasi lignin, bioetanol
ULTRAVIOLET MUTAGENESIS OF LOCAL ISOLATE Trichoderma sp. T065 FOR IMPROVING CELLULASES ACTIVITY (Mutagenesis Isolat Lokal Trichoderma sp. T065 menggunakan Ultraviolet untuk meningkatkan Aktivitas Selulase) Anindyawati, Trisanti; Jusuf, Eddy; Abimanyu, Haznan
JURNAL SELULOSA Vol 6, No 01 (2016): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1043.034 KB) | DOI: 10.25269/jsel.v6i01.65

Abstract

Mutagenesis of indigenous fungal isolates Trichoderma sp. T065 was achieved by UV light in a laminar air flow and UV crosslinker to increase cellulase activity. Thirty-four mutants were tested for their growth capacity in mineral agar with several carbon sources: Whatman filter paper no.1, 1% carboxymethylcellulose (CMC), 2% cellulose powder, 1% Avicel and 4% delignified oil palm empty fruit bunches (DOPEFB) with granule size of 200 mesh. Three mutants (UV-1.1, 1.2-UV and UV-1.3) showed bigger growth zone on cellulose substrate of 4% DOPEFB than that of wild type Trichoderma sp. T065. The highest cellulase activities were 0.65 FPU/mL and 0.57 FPU/mL from UV-1.1 and UV-1- 3, respectively higher than wild type that is equal to 0.038 FPU/mL.Keywords: Trichoderma sp. T065, mutations, UV light, carbon source, cellulase activityABSTRAKMutagenesis isolat lokal kapang Trichoderma sp. T065 dilakukan dengan sinar UV pada laminar air flow dan UV crosslinker untuk meningkatkan aktivitas selulase. Tiga puluh empat kapang mutan diuji kapasitas pertumbuhannya pada mineral agar dengan beberapa jenis sumber karbon yaitu kertas saring Whatman no.1, 1% carboxymethylcellulose (CMC), 2% serbuk selulosa, 1% avicel dan 4% tandan kosong sawit (TKS) dengan ukuran granula 200 mesh. Tiga mutan (UV-1.1, UV-1.2 dan UV- 1.3) mempunyai zona pertumbuhan yang lebih besar pada substrat selulosa dengan sumber karbon 4% TKS daripada isolat asli Trichoderma sp. T065. Aktivitas selulase tertinggi adalah 0,65 FPU/mL dan 0,57 FPU/mL berturut-turut dari mutan UV-1.1 dan UV-1.3 yang lebih tinggi dari isolat aslinya yaitu 0,038 FPU/mL.Kata kunci : Trichoderma sp. T065, mutasi, sinar UV, sumber karbon, aktivitas selulase  
Isolasi, Uji Aktifitas Antibakteri dan Identifikasi Senyawa Aktif Kapang Endofit dari Tanaman Belimbing Manis (Averrhoa carambola L.) Anindyawati, Trisanti; Dody, Priadi
Warta Industri Hasil Pertanian Vol 34, No 1 (2017)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1062.508 KB) | DOI: 10.32765/warta ihp.v34i1.4065

Abstract

ABSTRAK: Tanaman belimbing (Averrhoa carambola) memiliki potensi farmakologis, antara lain sebagai senyawa antimikroba. Kapang endofit isolat A 1.1 yang diisolasi dari ranting tanaman belimbing, diuji potensinya sebagai antibakteri terhadap Bacillus subtilis, Staphylococcus aureus dan Eschericia coli. Uji antibakteri dilakukan dengan metoda difusi cakram. Analisis GC-MS dilakukan terhadap ekstrak etil asetat kapang endofit A 1.1 untuk identifikasi komponen kimianya. Hasil uji antibakteri menunjukkan bahwa ekstrak etil asetat dan ekstrak kloroform mampu menghambat pertumbuhan bakteri uji. Ekstrak etil asetat mempunyai aktivitas DDH (7,5 mm) lebih besar dibanding ekstrak kloroform (6,10 mm) terhadap B. subtilis. Hasil analisis GC-MS menunjukkan bahwa ekstrak etil asetat kapang endofit A 1.1 mengandung 17 komponen kimia. Komponen utamanya adalah 1-Octadecene, 1-Dococene dan 1-Hexadecene.
IMPROVING THE QUALITY OF BREAD USING CASSAVA FLOUR SUBSTITUTION AND α-AMYLASE khusniati, tatik; Purnamasari, Elisa; Effendi, Supli; Anindyawati, Trisanti
Teknologi Indonesia Vol 39, No 3 (2016)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.054 KB) | DOI: 10.14203/jti.v39i3.271

Abstract

Bread in Indonesia generally used wheat flour as a basic material and it is an imported flour product up to now. On the other hand, cassava flour can be used as wheat flour alternative for substitution. This research was aimed to improve the quality of bread using cassava flour substitution and α-amylase. Variables used were wheat and cassava flour with comparison: 100:0, 95:5, 90:10, 85:15, and α-amylase with concentration: 0, 0.5, 1.0, 1.5%, respectively. The contents of water, ash, protein, carbohydrate, and salt were determinated by modifying the AOAC method. The pH of bread dough was measured and α-amylase activities were determined by Iodine method. The dough and substituted-bread volumes were measured, and organoleptic tests were conducted by training 20 panelists. Statistical analysis used factorial completely randomized experimental design (FCRD). The results show that the highest values of water, ash, protein and carbohydrate were found in bread of A4B2 (25.13%), A1B1 (0.97%), A1B2 (8.62%), A4B2 (47.89%), sequentially (P<0.05). A2B4 was bread with the best organoleptic value and biggest volume than the others with nutritional contents of water (25.10%), ash (0.87%), protein (7.98%), carbohydrate (46.89%), and salt (2.17%). The values of these nutritional contents was in scope of bread SNI standard. Cassava flour substitution and α-amylase affected significantly to organoleptic values of bread produced (P<0.05). The α-amylase activities of all treated bread were not significantly different (P<0.05). Based on volumes of bread dough and bread as well as organoleptic tests, the best bread is A2B4 (95% wheat flour using 5% cassava flour and 1.5% α-amylase).
The Evaluation of Substrates and Trichoderma sp. Isolates for Cellulase Production Triwahyuni, Eka; Aristiawan, Yosi; Ariani, Novita; Abimanyu, Haznan; Anindyawati, Trisanti
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 20, No 1 (2018)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (650.106 KB) | DOI: 10.14203/jkti.v20i1.384

Abstract

AbstractAs higher interest was on the lignocellulose-based or second generation bioethanol production, the research was then more focused on the production of cellulase, especially on the domestic enzyme. Trichoderma sp. is considered as one of the most efficient producer of cellulase. This study was conducted to investigate the performance of Trichoderma sp. on a variety of substrates to produce cellulase. Three types of substrate variations and three types of Trichoderma sp. were used in this experiment. The substrate used were wheat bran, rice bran and oil palm empty fruit bunches (EFBs), whereas Trichoderma sp. isolates were encoded as T004, T051 and T063. Production of cellulase was made by solid fermentation for 7 days. The analysis of cellulase activity was done by National Renewable Energy Laboratory (NREL) method for filter paper assay. The results showed that the type of substrate affected the performance of Trichoderma sp. All types of fungus produced cellulase on wheat bran substrate with activity of 0.52 FPU /ml for T004, 0.23 FPU/ml for T051 and 0.27 FPU /ml for T063. With the rice bran substrate and EFBs, only T004 could produce cellulase and the enzyme activity analyzed were 0.08 FPU /ml and 0.008 FPU/ml respectively. Optimation of the buffer addition on enzyme extraction process produces the highest activity 0.85 FPU/mL for T004 with wheat bran substrate. Keywords: cellulase, EFBs, rice bran , Trichoderma sp. , wheat bran
Characterization of Protease Crude Extract from Indigenous Lactic Acid Bacteria and the Protein Degradation Capacity in Local Tuber and Cereal Paste Flour Khusniati, Tatik; Nur Kasfillah, Nanda Sabbaha; Syafriana, Vilya; Zahara, Resti Sofia; Citroreksoko, Padmono; Sulistiani, Sulistiani; Anindyawati, Trisanti
Jurnal Kimia Terapan Indonesia (Indonesian Journal of Applied Chemistry) Vol 21, No 1 (2019)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (35.694 KB) | DOI: 10.14203/jkti.v21i1.419

Abstract

Protease hidrolyzed protein in flour in order to more digest by human ulcer. Lactobacillus plantarum B110 and Lactobacillus satsumensis are indigenous lactic acid bacteria that produce protease. The objective of this research is to characterization of protease crude extract from indigenous lactic acid bacteria and the protein degradation capacity in local tuber and cereal paste flour. Tuber and cereal flour used were purple sweet potato (Dioscorea alata), cassava (Manihot esculenta), rice (Oryza sativa), corn (Zea mays) and wheat (Triticum) as comparison. Proteaseactivity was tested by Horikoshi method (1971) and protein degradation was by formol titration. Research results showed that optimum activities and stabilities of Lactobacillus plantarum B110 were at pH: 7.5, 45oC and pH:5.0-8.0, 35-50oC, while that L. satsumensis EN 38-32 were at pH: 7.0, 40oC and pH:6.0-8.0, 20-45oC. Increases in protein degradation capacity of the paste flour additional proteases crude extract from L. plantarum B110 were 0.0838% (purple sweat potato), 1.3299% (cassava), 0.5834% (corn), 0.7499% (rice) and 1.5551% (wheat as comparison); while that L. satsumensis EN 38-32 were 0.20% (purple sweet potato), 0.32% (cassava), 0.87% (corn), 1.17% (rice). Based on increases in protein degradation capacity, protease crude extract from L. plantarum B110  and L. satsumensis EN 38- 32 were sequently better to hidrolyze protein of cassava and rice paste flour than thatother tuber and cereal.
RAPID ASSESSMENT OF DIVERSE TRICHODERMAL ISOLATES OF INDONESIAN ORIGIN FOR CELLULASE PRODUCTION Fahrurrozi, Fahrurrozi; Ratnakomala, Shanti; Anindyawati, Trisanti; Lisdayanti, Puspita; Sukara, Endang
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (649.7 KB) | DOI: 10.14203/ann.bogor.2010.v14.n1.39-46

Abstract

Trichoderma is a well -known candidate to be promoted as cellulase producer  for the hidrolysis of lignocelluloses that contain  in  woody  biomass. The  number of trichodermal  isolates  in our  laboratory collected  from diverse ecosystem types in Indonesia increases significantly during the last 5 years. It is our aim to assess the cultures for its ability in producing cellulase. Sixty-six trichodermal isolates used in this experiment are obtained from Biotechnology Culture Collection (BTCC), Research Center for Biotechnology, Indonesian Institute of Sciences(LIPI)  The 31 isolates were isolated from District of Liwa (South Sumatra, Indonesia) and the 35 isolates from District of Maros (South Sulawesi, Indonesia). The  isolates were screened qualitatively,  7  isolates  from  Liwa and 12  isolates from Maros showed cellulolytic activity. From the results of quantitative test, two strains  (ID08-T004 and ID08-T63) showed the higher cellulolytic activity among the selected strains, 133.5 and 133.5 U/ml, respectively.  These  extracellular  enzymes  were  characterized  their  temperature  and  pH  optimum.  The temperature  optimum  for  both  enzymes  was  the  same,  50?C,  with  activity  213.6  U/ml  for  enzyme  extracted from ID08-T004 and 197.3 U/ml for enzyme from ID08-T0063. The pH optimum was pH 5 of ID08-T004 with activity137.7 U/ml and pH 6 for ID08-T063 with activity 75.0 U/ml. The enzymes from ID08-T004 and ID08-T063 were stable in their temperature and pH optimal condition even after 90 minutes incubation with activity 179.0 U/ml and 86.7 U/ml, respectively. The enzyme stability was approximately 150 minutes for both enzymes in the temperature and pH optimum.   Key Words: Trichoderma, cellulase, Culture Collection