Articles

Found 4 Documents
Search

Sitotoksisitas Ekstrak Aspergillus fumigatus dari Daun Mekai (Albertisia papuana Becc.) terhadap Sel Kanker Payudara T47D dan MCF-7 Maritsa, Hasnaul; Moeljopawiro, Soekarti; Kasiamdari, Rina Sri
BIO-SITE |Biologi dan Sains Terapan Vol 1 No 1 (2015): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (681.348 KB)

Abstract

The previous studies showed that the Albertisia papuana Becc. root have cytotoxicity on breast cancer. The A. papuana root toxicity on breast cancer could not only by plant secondary metabolism, but may be also by secondary metabolism of endophytes. Aspergillus fumigatus is one of endophytes that have anticancer agent. Endophytes can be distributed dynamically in whole of plant organ, one of them are leaves. Therefore the objective of this studies were to know the presence of A. fumigatus in A. papuana leaves, and the cytotoxicity of their secondary metabolism on breast cancer cells. The sample of A. papuana were collected from Botanical Zoo of Bogor, while T47D and MCF-7 cell lines were obtained of Tropical Medicine’s Faculty, UGM. Isolation of endophytes was done by growing leaves extract on water agar 2 % medium. Secondary metabolism was extracted from fermented broth using in ethyl acetat and n-butanol. The cytotoxicity was perform by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The result showed that A. fumigatus assosiated with A. papuana leaves. Ethyl acetat extract from fermented A. fumigatus both on T47D and MCF-7 cell lines had lower (IC50. 50, 444 µg/ml and 59 µg/ml) than n-butanol (IC50. 103, 398 µg/ml and 127,188 µg/ml. It could be said that A. fumigatus from A. papuana leaves could induce cytotoxicity on T47D and MCF-7 breast cancer cells.
Sitotoksisitas Ekstrak Aspergillus fumigatus dari Daun Mekai (Albertisia papuana Becc.) terhadap Sel Kanker Payudara T47D dan MCF-7 Maritsa, Hasnaul; Moeljopawiro, Soekarti; Kasiamdari, Rina Sri
BIO-SITE |BIOLOGI Sains Terapan Vol 1, No 01 (2015): Bio-Site
Publisher : BIO-SITE |BIOLOGI Sains Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The previous studies showed that the Albertisia papuana Becc. root have cytotoxicity on breast cancer. The A. papuana root toxicity on breast cancer could not only by plant secondary metabolism, but may be also by secondary metabolism of endophytes. Aspergillus fumigatus is one of endophytes that have anticancer agent. Endophytes can be distributed dynamically in whole of plant organ, one of them are leaves. Therefore the objective of this studies were to know the presence of A. fumigatus in A. papuana leaves, and the cytotoxicity of their secondary metabolism on breast cancer cells. The sample of A. papuana were collected from Botanical Zoo of Bogor, while T47D and MCF-7 cell lines were obtained of Tropical Medicine’s Faculty, UGM. Isolation of endophytes was done by growing leaves extract on water agar 2 % medium. Secondary metabolism was extracted from fermented broth using in ethyl acetat and n-butanol. The cytotoxicity was perform by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The result showed that A. fumigatus assosiated with A. papuana leaves. Ethyl acetat extract from fermented A. fumigatus both on T47D and MCF-7 cell lines had lower (IC50. 50, 444 µg/ml and 59 µg/ml) than n-butanol (IC50. 103, 398 µg/ml and 127,188 µg/ml. It could be said that A. fumigatus from A. papuana leaves could induce cytotoxicity on T47D and MCF-7 breast cancer cells.   Keywords: cancer, Aspergillus fumigatus, secondary metabolism, Albertisia papuana Becc., cytotoxiciy
Penetapan Kadar Genistein Dalam Ekstrak Metanol BijiKedelai Glycine Max L. Merr. Varietas Grobogan MenggunakanMetode KLT Dan HPLC Aryani, Retno; Astuti, Pudji; Moeljopawiro, Soekarti; Nugroho, Laurentius Hartanto
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (572.279 KB)

Abstract

Soybean (Glycine max L. Merr.) is one of the foodstuffs that are oftenconsumed most of Indonesian. Soy contains phytoestrogens, whichhave chemical structures resemble the hormone estrogen in the body,namely the isoflavones, especially genistein. Genistein is known notonly have various beneficial physiological effects but also act as anendocrine disruptor is because estrogen can play a role as well asantiestrogen activity that affects the metabolism of sex hormones andrelated activities biologi. The objective of this study was to know thequality of genistein in soy extracts Grobogan varieties that have beenpeeled the husk with Thin Layer Chromatography (TLC) and thequantity of soy extract genistein levels Grobogan varieties that havebeen peeled the husk with a method of High Performance LiquidChromatography (HPLC). The method used for extraction ofisoflavones genistein is maceration. Soybean seed samples which havebeen hulled begins with removal of non polar compounds byextraction using n-hexane solvent extraction followed macerationusing 80% methanol. The methanol extract obtained fractionated witha stationary phase of silica gel GF 254, the mobile phase toluene-ethylacetate-aseton- formic acid 20: 4: 2: 1 (v / v). To determine whether ornot a compound genistein compounds in the extract by using acomparison standard genistein further Rf sample extract comparedwith the price of a standard Rf. While the determination of genisteincompound quantitatively with HPLC method genistein standard wear≥ 98% at a concentration of 2, 4, 6, 8, 10 ppm. The identification ofgenistein using Thin Layer Chromatography (TLC), shows the stainsthat have Rf of about 0.43. Results genistein content by HPLC showedpeaks of the chromatograms that have a retention time of about 2,050minutes. HPLC analysis results in three replication showed levels ofgenistein in soya bean varieties Grobogan was 1 g samples of soyextracts that have been flayed husk there was an average of 0.6356 mggenistein. In the soybean seed Grobogan varieties contains isoflavonesgenistein. Levels of genistein in 1 g of sample soy extracts Groboganvarieties that have been flayed epidermis was an average of 0.6356 mggenistein.
Analysis of whole cell protein profiles by SDS-PAGE to identify indigenous cellulose-producer acetic acid bacteria Sarkono, Sarkono; Moeljopawiro, Soekarti; Setiaji, Bambang; Sembiring, Langkah
Indonesian Journal of Biotechnology Vol 21, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (909.285 KB) | DOI: 10.22146/ijbiotech.27166

Abstract

This study was carried out to analyze the suitability of the identification of four indigenous cellulose-producing acetic acid bacterial isolates (ANG29, KRE65, ANG32 and SAL53) based on the analysis of whole cellular protein profiles against identification based on phenotypic traits. Whole cellular protein profiles were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. The whole cellular protein profiles obtained from sample isolates, were compared with reference isolates for species identification. The results showed that based on visual observations can be determined as much as 12 bands of protein with a molecular weight of 19,099 KDa up to 132.182 KDa. Based on the analysis of protein bands were detected visually, fourth indigenous cellulose- producing acetic acid bacterial isolates in the study had a higher similarity profile to the reference strain Gluconacetobacter xylinus BTCC 769 compared with other reference strains namely G. hansenii NBRC 14820T. This condition is consistent with the results of the identification of fourth cellulose producing acetic acid bacterial isolates based on phenotypic traits. Thus, the whole cellular protein profiles by SDS-PAGE technique can be used as a one of method to identification of cellulose producing acetic acid bacterial isolates.