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UJI BRINE SHRIMP LETHALITY TEST (BSLT) UMBI BAWANG TIWAI (ELEUTHERINE BULBOSA (MILL.) URB) DAN UJI TOKSISITAS AKUT FRAKSI AKTIF Lestari, Dwi; Kartika, Rudi; Marliana, Eva
Jurnal Riset Kefarmasian Indonesia Vol 1 No 1 (2019): Jurnal Riset Kefarmasian Indonesia
Publisher : APDFI (Asosiasi Pendidikan Diploma Farmasi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.051 KB) | DOI: 10.33759/jrki.v1i1.43

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Eleutherine bulbosa (Mill.) Urb come from the Iridaceae family, this species contains secondary metabolites in the form of flavonoids and quinones. This research method includes Brine Shrimp Lethality Test (BSLT) toxicity using Artemia salina Leach larvae to determine the LC50 value. This study used experimental methods pharmacologically using a completely randomized design in the same direction pattern in the selection of test animals, 25 animals used were white mice divided into 5 groups with 5 mice per group, with 4 hours observed to find out toxic symptoms and continued observation every 24 hours to see death. Testing of BSLT results showed that ethanol extract had LC50 toxicity = 66.68 ppm (very toxic category), n-hexane fraction had LC50 toxicity = 47.64 ppm (very toxic category), chloroform fraction had LC50 toxicity = 295.1 ppm ( toxic category), and the water fraction has a toxicity of LC50 = 194.54 ppm (very toxic category). The chloroform fraction is an active fraction. Acute toxicity test LD50 value according to the calculation method of Miller Tainter Probit (187,499 mg/KgBB), the method of calculation of Thompson Weil (182,810 mg/KgBB), how to calculate Indonesian Pharmacopoeia (187,068 mg/KgBB). Based on the three calculations, the LD50 value is in the medium category (50-500 mg/kgBB).
PENINGKATAN HASIL BELAJAR SISWA MENGGUNAKAN COOPERATIVE LEARNING TEKNIK INDEX CARD MATCH DI KELAS IV Marliana, Eva; Margiati, K Y; Halidjah, Siti
Jurnal Pendidikan dan Pembelajaran Khatulistiwa Vol 7, No 9 (2018): September 2018
Publisher : Jurnal Pendidikan dan Pembelajaran Khatulistiwa

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Abstract

AbstractThe general problem of research "Whether by using cooperative learning index card match techniques can improve student learning outcomes in learning the use of units of time in class IV Elementary School 11 Pontianak East?".Using descriptive method. The Class Action Research Form is collaborative. The subjects of the research are teachers as researchers and students of class IV which amounted to 24 people. Using direct observation techniques and documentation techniques, data collection tools of observation sheets and test instruments. Score data of teachers' ability to design and implement learning using cooperative learning index card match technique can improve learning outcomes of learners in learning class IV Mathematics. The study was conducted 3 cycles. Every cycle I meeting. The results achieved in this study the ability of teachers to design the learning cycle with an average score of 2.70, cycle II 3.36, cycle III 3.93. The ability of teachers to implement learning cycle I average 3.05, cycle II 3.53, cycle 3 3.81. Student learning outcomes in learning cycle I average 70.42, cycle II 82.29, cycle III 90.21. Implementation of cooperative learning index card match techniques in learning Mathematics can improve student learning outcomes fourth grade Elementary School 11 Pontianak East. Keywords:Learning Outcomes, Cooperative Learning Index Card Match Techniques, Mathematics.
UJI FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL BIJI KELENGKENG (Euphoria Iongan STEUD) DENGAN METODE PERENDAMAN RADIKAL 2,2-DIPHENIL-1-PICRYLHIDRAZYL (DPPH) Chatimah, Husnul; Saleh, Chairul; Marliana, Eva
JURNAL KIMIA MULAWARMAN Vol 9 No 2 (2012)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Kelengkeng fruit already well known for Indonesian society. Not only from the contents of the fruit, but Kelengkeng also have many other beneficial from the seed, leather, root and leaves that useful for human health. In this research, phytochemical and antioxidant activity test have been done. Antioxidant activity test was carried out with scavenging test to 2,2-diphenyl-1-picrylhidrazil (DPPH) radical. On antioxidant activity test, mix of extract and DPPH are incubated for 30 minutes at 37oC, then the absorbance measured at λ = 517 nm using spectrophotometer. The absorbance from sample, blank and negative control are converted as Antioxidant Activity Absorbance (AA%). Based on result of phytochemical etanol extract, Kelengkeng contains phenolic compounds. And based on antioxidant activity test with DPPH radical scavenging method by spectrophotometer, ethanol extract of Kelengkeng has antioxidant activity about EC50(72.23±2.55) while Vitamin C as comparison has antioxidant activity about EC50(6.07±0.36).Keywords: Kelengkeng, phytochemical, antioxidant activity and DPPH
STUDI KOMPUTASI BERBASIS DFT TERHADAP FENOMENA QUANTUM TUNNELING DALAM ISOMERISASI METILHIDROKSIKARBENA Awal, Jumaidil; Gunawan, Rahmat; Marliana, Eva
JURNAL KIMIA MULAWARMAN Vol 9 No 2 (2012)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

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Abstract

Density functional theory-based methods have been applied to predict the most possible one among the isomerizations of methylhydroxycarbene considering the probability of hydrogen tunneling occurrence. B3LYP/6-31+G(d,p) and M08-SO/6-31+G(d,p) methods were applied in all computations using GAMESS-US software. There were three steps of computation in this research. First, electronic structure computations of both equilibrium and transition compounds involved in all isomerization alternatives in order to obtain the optimum structures of the compounds. Second, vibrational computations of optimum transition structures to ensure that each of the respective structures is well on its potential energy surface. Third, tunneling analysis accomplished by intrinsic reaction coordinate (IRC) computatuins for all isomerization alternatives followed by tunneling probabilitycalculation using the Wentzel-Kramers-Brillouin (WKB) formula for methylhydroxycarbene isomerizations. The result of this research showed that the DFT methods successfully produced the optimum structure of each compound. Both DFT methods also successfully mapped all the intrinsic reaction coordinates. B3LYP/6-31+G(d,p) method gave tunneling probabilities of 3.55 x 10-19 for the isomerization into acetaldehyde and 3.30 x 10-20 for that into vinyl alcohol. While M08-SO/6-31+G(d,p) method gave tunneling probabilities of 2.38 x 10-23 for the isomerization into acetaldehyde and 4.79 x 10-23 for that into vinyl alcohol.Keywords: DFT, methylhydroxycarbene, hydrogen tunneling, isomerization
THREE PRENYLATED FLAVONOIDS FROM MACARANGA PEARSONII MERR. Marliana, Eva; Tjahjandarie, Tjitjik Sri; Tanjung, Mulyadi
PROSIDING SEMINAR KIMIA PROCEEDING OF INTERNATIONAL CONFERENCE 2015
Publisher : PROSIDING SEMINAR KIMIA

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Abstract

Three prenylated flavonoids, 4’-O-methyl-8-isoprenyleriodictiol (1), 4’-O-methyl-8-isoprenylnaringenin (2) and lonchocarpol A (3) have been isolated from the leaves of Macaranga pearsonii Merr.. The structure of compounds have been elucidated based on its spectroscopic data, including UV, 1D and 2D NMR, and HREISMS spectra.
Aktivitas Antioksidan dan Antimalaria Senyawa Fenolik dari Daun Macaranga beccariana Merr. Marliana, Eva; Saleh, Chairul; Hendra, Medi
JURNAL KIMIA MULAWARMAN Vol 15 No 2 (2018)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v15i2.611

Abstract

Macaranga beccariana Merr. merupakan tanaman endemik Kalimantan yang diketahui belum banyak penelitian mengenai aktivitas biologi dan senyawa bioaktif dari tanaman ini. Oleh karena itu, untuk mengetahui potensi dari M. beccariana maka pengujian aktivitas antioksidan dan antimalaria serta isolasi senyawa bioaktif dari tanaman ini perlu dilakukan. Pada penelitian ini, daun M. beccariana diekstraksi dengan metanol sehingga diperoleh ekstrak metanol. Kemudian, ekstrak tersebut dipisahkan dan dimurnikan sehingga diperoleh senyawa 1 dan 2. Berdasarkan elusidasi struktur dengan spektrometer UV, 1H dan 13C NMR, senyawa 1 dan 2 diketahui sebagai kelompok senyawa fenolik dan diidentifikasi strukturnya masing-masing sebagai asam 4-hidroksibenzoat dan asam 3,4-dihidroksibenzoat. Selanjutnya dilakukan uji aktivitas antioksidan dengan menggunakan DPPH (2,2-diphenyl-1-picrylhydrazyl) dan uji aktivitas antimalarial terhadap Plasmodium falciparum strain 3D7 dengan pewarnaan Giemsa. Kontrol positif yang digunakan dalam uji aktivitas antioksidan adalah rutin, sedangkan uji aktivitas antimalaria adalah klorokuin difosfat. Hasil uji menunjukkan bahwa ekstrak metanol M. beccariana dan senyawa isolasi (1 dan 2) adalah aktif untuk kedua uji tersebut. Sehingga dapat disimpulkan bahwa daun M. beccariana memiliki potensi sebagai agen antioksidan dan antimalaria.
ANALISIS KADAR ASAM SIANIDA DALAM SOLANUM MELONGENA L DAN SOLANUM QUITOENSE L SEBELUM DAN SESUDAH PEREBUSAN Gama, Sabaniah Indjar; Alimuddin, Alimuddin; Marliana, Eva
Journal of Tropical Pharmacy and Chemistry Vol 1 No 1 (2010): Journal of Tropical Pharmacy and Chemistry
Publisher : Faculty of Pharmacy, Universitas Mulawarman, Samarinda, Indonesia, 75117

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.638 KB) | DOI: 10.25026/jtpc.v1i1.10

Abstract

ABSTRACT   Analysis cyanide acid in Solanum melongena and Solanum quitoense L before and after boiling. Cyanide acid is esthabushed by argonometricm method (Volhard) with three times repetition, for each kinds of eggplants Solanum melongena L dan Solanum quitoense L. The research showed have endireated that before boiling the content of cyanide acid Solanum melongena L (Craigi eggplant 71,712 mg/kg, kopek eggplant 46,310 mg/kg, gelatik eggplant 0 mg/kg) and Solanum quitoense L (asam eggplant 30,434 mg/kg) after boiling, the cyanide acid to slop Solanum melongena (Craigi eggplant 19,321 mg/kg, kopek eggplant 6.620 mg/kg, gelatik eggplant 0 mg/kg and Solanum quitoense L (asam eggplant 3.445 mg/kg). There is significant content have been reduced of cyanide acid in Solanum melongena L dan Solanum quitoense L   Keywords: Solanum melongena dan Solanum quitoense L, Cyanide acid   ABSTRAK   Telah dilakukan analisis asam sianida dalam Solanum melongena dan Solanum quitoense L sebelum dan sesudah perebusan menggunakan metode Volhard dengan tiga kali pengulangan. Data hasil penelitian menunjukkan kandungan asam sianida sebelum perebusan Solanum melongena (terong Craigi 71,712 mg/kg, terong kopek 46,310 mg/kg, terong gelatik  0 mg/kg)  dan Solanum quitoense L (terong asam 30,434 mg/kg) sedangkan setelah dilakukan perebusan (terong Craigi 19,321 mg/kg,  terong kopek 6.620 mg/kg,  terong gelatik 0 mg/kg) and Solanum quitoense L (terong asam  3.445 mg/kg). Terdapat perbedaan kandungan penting yang dapat mengurangi kadar asam sianida dalam Solanum melongena L dan Solanum quitoense L.   Kata kunci: Solanum melongena dan Solanum quitoense L, Asam Sianida
ISOLASI DAN KARAKTERISASI SENYAWA TERPENOID DARI DAUN Macaranga beccariana Merr. Atmoko, Doni Prio; Marliana, Eva; Erwin, Erwin
JURNAL KIMIA MULAWARMAN Vol 16 No 1 (2018)
Publisher : Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30872/jkm.v16i1.670

Abstract

Isolasi dan karakterisasi senyawa terpenoid dari fraksi etil asetat Macaranga beccariana Merr. telah dilakukan. Proses pemisahan pada fraksi etil asetat menggunakan kromatografi kolom gravitasi dengan metode elusi gradien. Isolat yang diperoleh diuji kemurniannya dengan menggunakan kromatografi lapis tipis dengan berbagai eluen yaitu n-heksana, kloroform, metanol, aseton dan etil asetat dengan nilai Rf secara berturut-turut yaitu 0; 0,75; 0,80; 0,87 dan 0,97. Senyawa terpenoid yang berhasil diisolasi berbentuk kristal putih dengan massa 0,0119 gram. Analisis dengan UV menghasilkan 1 puncak pada l 207,03 nm. Analisis senyawa dengan FT-IR mengindikasikan gugus fungsi -OH, C-H alifatik, C=C alifatik, C-O dan C=O. Berdasarkan dari hasil spektrum UV dan FT-IR maka senyawa isolat diduga mengandung senyawa terpenoid.
SCREENING BACTERIAL OF ENDOPHYTIC PRODUCING AMYLASE, LIPASE AND PROTEASE FROM LEAVES OF Macaranga hullettii King ex Hook.f. Pricilia, Sherly; Astuti, Winni; Marliana, Eva
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

The objective of this study was to screening test of endophytic bacteria producing amylase, lipase and protease from leaves Macaranga hullettii King ex Hook.f. Endophytic bacteria isolation was performed on nutrient agar (NA) and 21 single colonies of endophytic bacteria were obtained. Endophytic bacteria that all of the endophytic bacteria positive producing enzym, 8 of the endophytic bacteria positive amylase, 7 of the endophytic bacteria positive lipase and 4 of the endophytic bacteria positive protease.  
PHYTOCHEMICAL TEST ON METHANOL EXTRACT OF LEAF OF Macaranga hullettii King ex Hook.f. Rismawati, Rismawati; Marliana, Eva; Daniel, Daniel
JURNAL ATOMIK Vol 3 No 2 (2018)
Publisher : Jurusan Kimia FMIPA UNMUL

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Abstract

Uji Fitokimia Macaranga hullettii King ex Hook.f telah dilakukan. Penelitian ini bertujuan untuk mengetahui metabolit sekunder menggunakan Kromatografi Lapis Tipis  dari ekstrak metanol pada daun Macaranga hullettii. Berdasarkan hasil skrining fitokimia, diketahui bahwa ekstrak metanol mengandung senyawa alkaloid, steroid/triterpenoid, polifenol dan flavonoid.