Eliawardani Eliawardani, Eliawardani
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Histopathological Changes of Egg Cells in the Uterine of Ascaridia galli after Treatment with Extract of Veitchia merrillii Nuts Balqis, Ummu; Muslina, Muslina; Hambal, Muhammad; Athaillah, Farida; Muttaqien, Muttaqien; Azhar, Azhar; Ismail, Ismail; Rastina, Rastina; Eliawardani, Eliawardani; Harris, Abdul; Hamzah, Abdullah; Vanda, Henni; Darmawi, Darmawi
Majalah Obat Tradisional Vol 22, No 3 (2017)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (878.758 KB) | DOI: 10.22146/mot.31544

Abstract

The aim of the study was to observe the histopathological changes of the entire egg cells in the uterine of Ascaridia galli worms treated with crude ethanolic extract of Veitchia merrillii nuts. Histopathological changes of the entire egg cells were observed to describe that the activity of extract V. merrillii nuts interfered the reproduction process could decrease the quantity and quality of A. galli eggs. The experiments consisted of 16 head A. galli female adult worms divided into four groups, contained four worms of each, and maintained in 0.9% phosphate buffered saline (PBS). First group, a negative control experiment consisted of A. galli in 0.9% PBS. Second group, concentration of 15 mg/mL albendazole was used as a positive control. Third group and fourth group, in vitro exposure of the worms to the crude ethanolic extract V. merrillii nuts at a concentration of 25 mg/mL and 75 mg/mL, respectively. The histopathological changes of egg cells in the uterine region of stained worms was evaluated by light microscopic examination at 40x magnification. The results showed that shrinkage and disintegration of the entire egg cells in the breakage of uterus. The results indicate that the possible use of the plant as a potential anthelmintic against A. galli, the intestinal nematode parasite of domestic fowl.
AKTIVITAS ANTELMINTIK BIJI Veitchia merrillii TERHADAP Ascaridia galli SECARA IN VITRO Hamzah, Abdullah; Hambal, Muhammad; Balqis, Ummu; Darmawi, Darmawi; Maryam, Maryam; Rosmaidar, Rosmaidar; Athaillah, Farida; Muttaqien, Muttaqien; Azhar, Azhar; Ismail, Ismail; Rastina, Rastina; Eliawardani, Eliawardani
Majalah Obat Tradisional Vol 21, No 2 (2016)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.642 KB) | DOI: 10.22146/tradmedj.12818

Abstract

Tujuan penelitian ini adalah mengevaluasi aktivitas antelmintik ekstrak etanol biji Veitchia merrillii terhadap nematoda intestinal, Ascaridia galli. Metode fitokimia dilakukan untuk menguji keberadaan jenis kimiawi di dalam ekstrak. Efek dari ekstrak tersebut ditentukan melalui in vitro berdasarkan hambatan pergerakan dan kematian cacing. Sebanyak empat ekor cacing masing diperlakukan triplikat dalam phosphate buffered saline, 25 mg/mL, dan 75 mg/mL larutan ekstrak V. merrillii, dan 15 mg/mL albendazole. Pergerakan cacing diamati pada interval 9, 18, 27, dan 36 jam. Kematian cacing ditentukan berdasarkan ada tidaknya pergerakan badan, bagian kepala, dan ekor cacing. Kami menemukan bahwa larutan ekstrak V. merrillii mengandung tanin, alkaloid, flavonoid, triterpenoid, saponin, tetapi tidak mengandung steroid. Berdasarkan percobaan in vitro, penggunaan konsentrasi 25 dan 75 mg/mL ekstrak V. merrillii menunjukkan efek antelmintik. Ekstrak V. merrillii tersebut hanya efektif pada konsentrasi 75 mg/mL. Kajian tersebut mengindikasikan bahwa antelmintik berbasis herbal untuk mengendalikan A. galli berpontesi dikembangkan.
20. Ectoparasites Identification of Stingrays Fish (Dasyatis Sp.) At Peunayong Fish Market, Banda Aceh Muttaqien, Muttaqien; Khaira, Arini Ulfa; Winaruddin, Winaruddin; Eliawardani, Eliawardani; Hambal, Muhammad; Azhar, Al
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.652 KB) | DOI: 10.21157/j.med.vet..v13i1.3525

Abstract

The aim of this research was to observe the histological of the thymus in tegal duck (Anas javanicus) according to the variation of ages. The animals used in this research were one month, two months and three months old of tegal duck. The thymus of each duck was analyzed by Hematoxylin-Eosin (HE) method and then observed under a light microscope. The result of the research performed differences in thymus structure of these three ages. The cortex had increased at the age of 2 months and decreased at the age of 3 months. The longest diameter of medulla at the age of 3 months was followed by ages 2 months and 1 month of tegal ducks.  The distribution of Hassall's body had increased at every age level. So, the cortex thickness decreased at the age of 2 months to 3 months, while the diameter of the medulla was getting longer.
17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate Dewi, Maryulia; Darmawi, Darmawi; Helmi, T. Zahrial; Erina, Erina; Gani, Basri; Eliawardani, Eliawardani; Azhar, Azhar
Jurnal Medika Veterinaria Vol 13, No 1 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.621 KB) | DOI: 10.21157/j.med.vet..v13i1.8884

Abstract

The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline  had the ability to inhibit the biofilm of Staphylococcus aureus.
29. IDENTIFICATION OF ECTOPARASITES IN STINGRAYS FISH (DASYATIS SP.) SOLD AT THE WET MARKET OF PEUNAYONG, BANDA ACEH Khaira, Arini Ulfa; Muttaqien, Muttaqien; Winaruddin, Winaruddin; Eliawardani, Eliawardani; Hambal, Muhammad; Azhar, Al
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.3547

Abstract

This study was conducted to identify ectoparasites of stingrays fish (Dasyatis sp.) sold at the wet market of Peunayong, Banda Aceh. A total of 15 stingrays were used as samples. Examination of ectoparasites on the surface of the body was conducted using scraping method and those on the gills and fins was performed using native method. Identification of ectoparasites obtained was done microscopically. The results revealed that two ectoparasites Caligus sp. were found in the gills of one out fifteen stingrays examined. The prevalence of Caligus sp. was 6.67%.
38. DENSITY FIGURE OF AEDES SPP LARVAE IN JEULINGKE VILLAGE SYIAH KUALA SUBDISTRICT BANDA ACEH Athailah, Farida; Hanafiah, Muhammad; Prapanca, Eka Ichsan; Riandi, Lian Varis; Eliawardani, Eliawardani; Muttaqien, Muttaqien
Jurnal Medika Veterinaria Vol 13, No 2 (2019): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v13i2.14814

Abstract

This study was aimed to determine the level of Density Figure of Aedes spp mosquito larvae with parameter House Index (HI) Container Index (CI), and Breteau Index (BI) at Jeulingke Village, Syiah Kuala Subdistrict, Banda Aceh. This research was conducted by using Single Larva Method. Single Larvae Method is a method used to take a single larva by using a plastic scoop on each container that is found to have a larva and visually observed by using a flashlight. The sample used consisted of 120 houses taken randomly from 6 hamlets. The larval data were obtained from various types of containers tabulated into tabular form, then the larvae index was calculated to determine the density of the larvae based on the Density figure criteria. The results showed that from 120 houses found 702 containers in Jeulingke Village with 20 positive larval houses and 20 containers that positively contained larvae such as 19 bath containers and used tires by 1 container so that the index of larvae obtained with House Index is 17% , Container Index of 3%, Breteau Index 20, so as to obtain Density Figure value in the range of numbers 2-4. The results show that the Density Figure in Jeulingke Village is in the medium category.
Motilitas Ascaridia galli Dewasa dalam Larutan Ekstrak Etanol Biji Palem Putri (Veitchia merrillii) Balqis, Ummu; Darmawi, Darmawi; Maryam, Maryam; Muslina, Muslina; Hamzah, Abdullah; Daud, Razali; Hambal, Muhammad; Rinidar, Rinidar; Harris, Abdul; Muttaqien, Muttaqien; Azhar, Azhar; Eliawardani, Eliawardani
Jurnal Agripet Vol 16, No 1 (2016): Volume 16, N0. 1, April 2016
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.142 KB) | DOI: 10.17969/agripet.v16i1.3022

Abstract

ABSTRAK. Tujuan penelitian ini untuk mengetahui motilitas Ascaridia galli dewasa dalam ekstrak etanol biji Veitchia merrillii. Ekstrak etanol V. merrillii dianalisis fitokimia. Sebanyak 16 ekor cacing A. galli dewasa dibagi kedalam empat kelompok. Cacing pada kelompok pertama adalah kelompok tanpa perlakuan. Cacing pada kelompok kedua diberi 0,6 mg/ml levamisole. Cacing pada masing-masing kelompok ketiga dan keempat diberi 50 dan 100 mg/ml crude ekstrak biji V. merrillii. Motilitas A. galli ditentukan dalam skor persentase setelah 12, 24, 36 jam dengan menggunakan kriteria: 3 (badan bergerak), 2 (hanya sebagian badan bergerak), 1 (tidak bergerak tetapi masih hidup), 0 (mati). Hasil fitokimia V. merrillii mengandung alkaloids, saponins, tannins, flavonoids, terpenoids. Ekstrak biji V. merrillii dosis 100 mg/ml secara in vitro dapat mempersingkat selama 12 jam waktu motilitas cacing A. galli dewasa. Penelitian ini mengindikasikan potensi anthelmintik berbasis herbal untuk pengendalian A. galli.  (Motility of Ascaridia galli adult worms in vitro in ethanolic extracts of Nuts Veitchia merrillii) ABSTRACT. The purpose of this research was to know the motility of Ascaridia galli adult worms in aqueous ethanolic extracts of nuts Veitchia merrillii. The ethanolic extract of the V. merrillii was analyzed. Amount of sixteen head A. galli adult worms were divided into four groups. The first group, worms were left as un-treated normal controls. The second group, worms were treated with concentrations of 0,6 mg/ml levamisole. The third and fourth group, worms were treated with crude aqueous ethanolic extract of 50 and 100 mg/ml concentrations nuts of the V. merrillii, respectively. Motility of A. galli were determined after 12, 24, 36 hour by mean of persentage scored using the following criteria: 3 (moving whole body), 2 (moving only parts of the body), 1 (immobile but alive), and 0 (died). The result of phytochemical V. merrillii contains alkaloids, saponins, flavonoids, tannins, and terpenoids. V. merrillii nuts extract concentrations of 100 mg/ml in vitro can shorten the time motility A. galli adult worms for 12 hours. The study indicated the potential for developing herbal-based anthelmintics to control A. galli.