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Analisis Filogenetik Mangifera odorata Sumatera Tengahdan Kerabatnya Menggunakan Gen rbcL JULIANTARI, ERWINA; , FITMAWATI; SOFIYANTI, NERY
Jurnal Riau Biologia Vol 1, No 2 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Mangifera Sumatera bagian Tengah memiliki kemampuan unik dalam beradaptasi pada wilayah dengan curah hujan yang tinggi, sehingga berpotensi sebagai sumber plasma nutfah Sumatera. Secara morfologi genus Mangifera cukup sulit dibedakan karena tingginya plastisitas morfologi. Mangifera odorata diduga sebagai spesies hibrid dari M. indica dan M. foetida, sehingga diperlukan analisis untuk memperkuat status taksonomi M. odorata. Tujuan penelitian ini untuk merekonstruksi hubungan kekerabatan M. odorata dan kerabatnya berdasarkan gen rbcL. Rekostruksi menggunakan program PAUP* Versi 4.0b10 dengan metode maximum parsimony (MP). Kladogram dengan metode MP membentuk klad monofiletik dengan dua klad utama Mangifera. Klad pertama yaitu M. indica dan klad kedua terdiri dari M. foetida dan M. odorata. Kladogram mendukung asumsi bahwa M. odorata adalah hibrid dari M. foetida dan M. indica. 
Identifikasi Jamur Penyebab Penyakit Pascapanen pada Beberapa Komoditas Bahan Pangan PRATIWI, NOVA WAHYU; JULIANTARI, ERWINA; NAPSIYAH, LUTFI KHOTUN
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Food commodities can actually be stored for a long term after harvesting, but there are some constraintsof decrease in the quality of the fruit because of several postharvest diseases caused by microorganismssuch as fungi, bacteria, and viruses. The most frequently postharvest diseases trigger found is a group ofpathogenic fungi. The results of identification of fungi causing disease in several types of fruitpostharvest which were performed by blotter test method and medium PDA showed that Colletotricumcapsici fungi was found in chili, Rhizoctonia solani in bananas and tomatoes, Amerosporium sp. inpapaya, Pythium sp. on bananas, Fusarium solani on mango, banana, and papaya, and Phomopsis sp. ontomatoes.Keywords: fungi, food commodity, identification, postharvest diseases
The Potential of Wild Mango Leaves from Sumatera as the Immunostimulant Agent Fitmawati, Fitmawati; Juliantari, Erwina; Saputra, Agus; Sinaga, Patar; Roza, Rodesia Mustika; Isda, Mayta Novaliza
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.16549

Abstract

Wild Mango from Sumatera is potentially new immunostimulant. It contains mangiferin, which is potentially become the immunostimulant candidate. This study aimed to prove the immunostimulatory effect of wild mango leaves extract on white rat peritoneum fluid induced by Staphylococcus aureus bacteria. The immunostimulatory effect was determined based on the activity and capacity of macrophage phagocytosis. This study used 54 male white rats about 130 g - 290 g. Based on the results,the highest phagocytosis activity of macrophage cells was found in ethanol extract of Mangga Hutan leaves dose 2 with a percentage of phagocytosis activity was 84%. The highest phagocytosis capacity of the macrophage cells was also found in ethanol extract of Mangga Hutan leaves dose 2 with an average phagocytic capacity of 171.67 from 50 active macrophages. Results of this study indicated that wild mango leaves from Sumatera has the potential immunostimulant activity. This indicates that the wild mango have the potential for therapeutic efficacy for the prevention of degenerative diseases caused by immune deficiencies.
VARIASI INFRASPESIES MACANG (Mangifera foetida) BERDASARKAN SEKUEN GEN rbcL Rafidah, Nurul; Fitmawati, Fitmawati; Juliantari, Erwina; Sofiyanti, Nery
Al-Kauniyah: Jurnal Biologi Vol 12, No 1 (2019): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v12i1.5516

Abstract

AbstrakMacang (Mangifera foetida) adalah jenis mangga kosmopolit dengan vigoritas yang kuat. Macang banyak ditemukan di perkarangan dan kebun dan bersifat semi liar. Penurunan luas lahan seperti deforestasi dapat mengakibatkan keanekaragaman kultivar macang juga mengalami penurunan secara cepat. Penelitian ini bertujuan untuk menganalisis dan merekonstruksi hubungan kekerabatan antar kultivar macang menggunakan sekuen gen rbcL. Sampel berasal dari koleksi hasil eksplorasi mangga Sumatera bagian Selatan, yaitu Provinsi Bengkulu, Lampung dan Sumatera Selatan. Ekstraksi DNA dilakukan dengan menggunakan metode CTAB yang dimodifikasi, kemudian DNA diamplifikasi dengan menggunakan primer spesifik rbcL forward dan reverse, dan dilakukan sekuensing serta analisis filogenetik. Rekontruksi pohon filogenetik menggunakan program PAUP* versi 4.0b10 dengan metode Maximum Parsimony (MP) dan Neighbor Joining (NJ). Kladogram dengan MP diperoleh dua klad, yaitu pada klad I terdiri dari kultivar Macang lonjong dan Macang lado, sedangkan klad II terdiri dari kultivar Macang bulat. Berdasarkan metode NJ, diperoleh Macang bulat memiliki jarak genetik lebih panjang sehingga dianggap sebagai individu yang lebih primitif daripada kultivar yang lain. Dengan demikian, dari penelitian ini diperoleh informasi dan bukti dari status taksonomi kultivar macang.Abstract Mangifera foetida is a species of cosmopolitan mango with strong vigor. Some M. foetida are found in some front houses and gardens and has a character of semi-wild in its cultivation. Decreases in land area, such as deforestation, can lead to a rapid decline in their diversity of cultivars. This study aimed to analyze and reconstructs the phylogenetic relationship among M. foetida cultivars using the rbcL gene sequences. Samples were collected from the exploration within the area of South Sumatra, such as Provinces of Bengkulu, Lampung and South Sumatra. The DNA extraction was carried out using the modified CTAB method, followed by DNA amplification using rbcL-specific primers, sequencing, and phylogenetic analysis. The phylogenetic trees were reconstructed using the PAUP* version 4.0b10 by using the method of Maximum Parsimony (MP) and Neighbor Joining (NJ). Cladogram of the MP tree showed two clades that the clad I consisted of M. foetida (Macang lonjong) and M. foetida (Macang lado) cultivars, whereas clad II consisted of M. foetida (Macang bulat) cultivar. The NJ tree showed that M. foetida (Macang bulat) has a longer genetic distance so it is considered as a more primitive cultivar than others. Therefore, information and evidences from the taxonomic status of the M. foetida cultivars were obtained from this study.
Histological Study of White Rats Kidney Following The Consumption of Obat Pahit From Riau Archipelago Fitmawati, Fitmawati; Saputra, Agus; Titrawani, Titrawani; Juliantari, Erwina; Kusumo, Awal Prichatin
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.15065

Abstract

Obat Pahit from Lingga Malay ethnic is a traditional medicine which has believed by the local people to maintain the body stamina (immunomodulators and antioxidants). This study aimed to investigate the effect of Obat Pahit Potion on kidney histological structure of white male rats (Rattus norvegicus). This research was an experimental study with a Randomized Complete Block Design consisted of 15 treatments and 3 replications. The experiment groups consisted of control groups (Stimuno, distilled water, and CMC-Na 1%) and three treatment groups of Obat Pahit Potion (Kalan, SP4, Linau) with 4 different dosages. The samples of a kidney of white rats were prepared for histological observation using paraffin method and Hematoxylin-Eosin staining. The results of this study showed that three kinds of Obat Pahit with four different dosages showed the expansion of glomerular cells on kidney tissue. Damage that occurs in the kidneys is still in small amounts and normal range which is less than 25%. The results of this study provide information for the society that the consumption of Obat Pahit Potion will not cause toxicity effect on the kidney.