ANAK AGUNG NGURAH GEDE SUWASTIKA, ANAK AGUNG NGURAH GEDE
Jurusan/Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana

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KAJIAN PEMANFAATAN LIMBAH SABUT KELAPA MENJADI LARUTAN MIKROORGANISME LOKAL DHARMA, PUTU ANANTA WIDHIA; SUWASTIKA, ANAK AGUNG NGURAH GEDE; SUTARI, NI WAYAN SRI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.2, April 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Study of Coconut Husk Waste Utilization Become Local Microorganism Fermented Solution The coconut husk has the most component in the coconut fruit, it cover 35 % of their composition. The research aim to understand the impact of the substrate dose, fermentation period and interaction between factors in quality measurement of local microorganism solution. This research use randomized block design and factorial treatment arrangement. Parameter measurement in research were C organic, total N, C/N ratio, available P and K, C/P ratio, pH, salinity, total bacterial population and total fungi population. The result of the research show that significant interaction between factor with exception on total N. Overall dose of substrate show that the 300 grams and three weeks fermentation has the best result.
APPLICATION OF TRICHODERMA SPP. AND LIGNOHUMATE TO SUPPRESS A PATHOGEN OF CLUBROOT (PLASMODIOPHORA BRASSICAE WOR.) AND PROMOTE PLANT GROWTH OF CABBAGE Suada, I Ketut; Suwastika, Anak Agung Ngurah Gede; Pradnyana, I Kadek Ngestika; Shchegolkova, Nataliya; Poloskin, Rodion; Gladkov, Oleg; Yakimenko, Olga; Stepanov, Aleksey
International Journal of Biosciences and Biotechnology Vol 6 No 2 (2019)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2019.v06.i02.p01

Abstract

The high economic value of cabbage crop leads farmers to make various efforts to suppress the pathogen of club root which is detrimental to plants. Efforts that need to be put forward must be environmentally safe. One way that is environmentally friendly is to control a pathogen biologically using antagonistic microbes. Therefore, the use of microbes such as Trichoderma which has been widely studied is important because it was able to suppress clubroot incidence and stimulate plant growth as well. Meanwhile, the need for plant nutrients to maximize plant growth requires an input of organic materials such as lignohumate which preserve soil nutrients, improve soil structure and increase plant resistance to biotic and abiotic stresses. The previous study on this scheme found an effective indigenous Trichoderma to suppress clubroot, therefore it is used in the current study. The objective of this study was to find out a combination treatment of Trichoderma and lignohumate which can suppress clubroot and increase plant growth. This experiment used a Randomized Block Design with 2 factors and 3 replications. Trichoderma concentration consisted of 3 levels, namely 0 spores (control), 1x106 spores. 2x106 spores, and 3x106 spores per plant which were suspended in 150 ml of water. The lignohumate treatments were 0.01, 0.02, 0.05, 0.1, 0.5, 1.0, and 2%. The results showed that lignohumate treatment was interact with Trichoderma population number on disease incidence, total clubroot, root dry weight, but not to canopy dry weight. The most suitable combination of treatments was the Trichoderma population of 3x106 spores (15 g) in combination to lignohumate of 0.5%.  This combination resulted in the lowest disease incidence, the lowest total clubroot, root dry weight, and the highest canopy dry weight. The higher the lignohumate concentration up to 0.5%, the higher the number of microbes (fungi and bacteria) growth, howeverit decreased above the concentration of 0.5%.
ISOLATION AND IDENTIFICATION OF PSEUDOMONAS SPP. TO CONTROL PLASMODIOPHORA BRASSCIAE, THE PATHOGEN OF CLUBROOT DISEASE ON CABBAGE Suada, I Ketut; Suwastika, Anak Agung Ngurah Gede
International Journal of Biosciences and Biotechnology Vol 4 No 2 (2017)
Publisher : Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/IJBB.2017.v04.i02.p01

Abstract

Clubroot is very detrimental disease to cabbage production so as farmers work on various efforts to control it. The use of fungicides not only ineffective but also pollute the environment, therefore biological control system need to be pursued. The use of antagonistic agents such as Pseudomonas has been widely studied and known effective in suppressing various pathogens. Therefore it is worth trying its effectiveness against Plasmodiophora brassicae, a pathogen of cabbage. The purpose of this study was to obtain indigenous Pseudomonas which effectively suppress the pathogens and may also increase plant growth. Microbes were isolated from the cabbage area using the Kings'B medium with multilevel dilution. All isolates were tested for their effectiveness in pots in a Completely Randomized Design with a concentration of 1.5x106 CFU (Colony Forming Unit) per pot. The variables observed were plant growth, number of club roots, and percentage of disease incidence. Fourteen isolates of Pseudomonas were isolated. Three Pseudomonas isolates were found most effective at suppressing clubroot disease and increasing plant growth. The best isolate obtained was Pseudomonas-6, followed Pseudomonas-9, and Pseudomonas-8. 
DINAMIKA AMONIUM DAN NITRAT LAHAN SAWAH LATOSOL PADA BUDIDAYA KONVENSIONAL PADI LOKAL DAN HIBRIDA DI SUBAK JATILUWIH PERMATASARI, GITA YUNI; KESUMADEWI, ANAK AGUNG ISTRI; SUWASTIKA, ANAK AGUNG NGURAH GEDE
Agrotrop: Journal on Agriculture Science Vol 9 No 2 (2019)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Ammonium Dynamics and Latosol Wetland Nitrates in Conventional Cultivation of Local and Hybrid Rice in Jatiluwih Subak. Plants absorb nitrogen in the form of ammonium (NH4+) and nitrate (NO3-). Each sample was analyzed for its ammonium and nitrate levels using macro Kjeldahl method. The results showed that the concentration of ammonium and nitrate in soil of the two rice fields were similar. The ions contents were as following: nitrate (2,67%) and ammonium (2,67%) in local rice, while those in hybrid rice fields were amounted of 1,48% and 4,59% respectivelly. The concentration of ammonium and nitrate on 21 DAC in soil samples remained low and rose at 28 DAC, then decreased at 56 DAC. It means that the times of fertilizing always done by the farmers were not appropriate.The content of ammonium and nitrate in water phase much lower than that in soils for both types of rice plants. The highest concentration of ammonium in local rice fields was 0,093% at the time of tillage and the lowest 0% on 21 DAC, while the highest nitrate concentrations in local rice water was 0,37% at 70 DAC and the lowest was 0% at of 21 DAC. The highest ammonium concentrations in hybrid rice 0,33% at 14 DAC, and the lowest 0% at 42 HST, while the highest nitrate content 0,47% at 42 DAC and the lowest 0% at the time of tillage. The fertilizing times recommended based on the findings of this reaserch is at 10-15 DAC for both rice types and another subsquent fertilization on 60-65 DAC for local rice and 40-45 DAC in hybrid rice.
ANALISIS KUALITAS LARUTAN MIKROORGANISME LOKAL DAUN GAMAL (GLIRICIDIA SEPIUM) PADA BEBERAPA WAKTU INKUBASI SUWASTIKA, ANAK AGUNG NGURAH GEDE; SUTARI, NI WAYAN SRI; MURIANI, NI WAYAN
Agrotrop: Journal on Agriculture Science Vol 5 No 2 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Analysis of Local Microorganism Leaf Gamal (Gliricidia Sepium) Solution Quality onthe Fermentation Period. The study aims to determine the quality of local microorganismsolution under the influence of concentration leaves of Gliricidia (Gliricidia sepium) andfermentation period. This research was conducted at the Soil Laboratory, Faculty ofAgriculture, UdayanaUniversity. The design of this research used a randomized block designfactorial pattern of two factors. The first factor was the concentration of Gliricidia leavesconsisted of 0, 100, 300 g, and the second factor was the fermentation period, consists of 1,2,and 3 weeks. Results of statistical analysis showed a highly significant interaction betweenthe concentration factor and fermentation period of Gliricidia leaves the parameters observed.Concentration of 30% (300 g) Gliricidia leaves with a two-week fermentation period has thebest effect on the quality of the biological properties of microorganisms local solution with atotal population of bacteria (9.5 x 107 SPK mL-1) and total fungus (1.9 x 106 SPK mL-1),while the best quality chemical properties of the solution of local microorganisms present in aconcentration of 30% (300 g) gliricidia leaves with a three-week fermentation period with thecontent of total-N (1.59%), pH (6.03), and organic-C (3.35%). Concentration of 30% (300 g)Gliricidia leaves with fermentation period of two weeks, it can be used as a biologicalfertilizer and a concentration of 30% (300 g) Gliricidia leaves with a three-week fermentationperiod, could be used as organic manure.
DINAMIKA AMONIUM DAN NITRAT PADA LAHAN SAWAH SEMI ORGANIK UNTUK TANAMAN PADI LOKAL DAN HIBRIDA DI SUBAK JATILUWIH KABUPATEN TABANAN KHOTIMAH, KHOSNUL; SUWASTIKA, ANAK AGUNG NGURAH GEDE; ATMAJA, I WAYAN DANA
Agrotrop: Journal on Agriculture Science Vol 10 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Dynamics Of Ammonium And Nitrate In Semi Organic Rice Fields For Local And Hybrid Rice Plants In Subak Jatiluwih, Tabanan Regency. The study was conducted to determine the dynamics of ammonium and nitrate and time subsequent fertilization in paddy fields with local and hybrid rice. Soil and water samples in two replications were taken each the two paddy fields periodically, namely during the tillage (DTT), 0, 7, 14, 21, 28, 42, 56, 70, 84, 98, and 112 days after planting (DAP). Ammonium and nitrate levels were analyzed by Macro Kjeldahl method. The results showed that the dynamics of ammonium and soil nitrate were different in two rice varieties. The highest ammonium content of local rice at the age of 7 DAP rice and the highest nitrate content at age 14 DAP. Ammonium and nitrate levels are highest in hybrid rice fields at 28 DAP rice age. The lowest ammonium and nitrate levels in two different varieties of rice land, namely the lowest local rice varieties at the age 28 DAP, whereas in hybrid rice land at age of 98 DAP. The results of this study indicate that supplementation of urea can be carried out at the age of 21 and 98 DAP for local rice varieties, and at the age of 14 DAP and 42 DAP for hybrid rice varieties.