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PERBAIKAN MUTU GENETIK MELALUI SISTEM GRADING TERNAK DALAM UPAYA MENUNJANG PROGRAM PEMULIAAN BERBASIS DIGITAL Irianto, Apri; Gunawan, Asep; Muladno, Muladno
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 7, No 1 (2020): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v7i1.8693

Abstract

ABSTRAKPengembangan sapi potong di Indonesia telah berkembang dibeberapa daerah dengan menerapkan teknologi dan inovasi peternakan melalui Sekolah Peternakan Rakyat (SPR). Penelitian ini bertujuan memperbaiki mutu genetik sapi PO melalui seleksi berdasarkan sistem grading untuk pembibitan dan perbanyakan sapi, serta melakukan pemantauan perkembangannya dengan program digitalisasi. Penelitian dilakukan melalui pendataan kuantitatif dan kualitatif dengan sistem wawancara langsung ke para peternak menggunakan kuisioner. Parameter penelitian diantaranya karakteristik eksternal, silsilah sapi, dan ukuran tubuh seperti bobot badan dan lingkar dada. Analisis data menggunakan Analisis  Deskriptif dan pendugaan model regresi linier. Hasil penelitian menunjukkan bahwa jumlah sapi di Desa Sekaran  lokasi Sekolah Peternakan Rakyat sebanyak 971 ekor dengan populasi terbanyak di dusun Ngantru yaitu sebesar 82%. Sistem perkawinan  sapi yang sering diterapkan yaitu kawin suntik (inseminasi buatan) sebesar 94% dan 6% dengan perkawinan alami. Produktivitas sapi PO cukup tinggi dengan ukuran lebar dada berkisar 154?165 cm dan bobot badan berkisar 282?309 kg. Body condition score (BCS) sapi PO berada pada kisaran 2?3 di skala 5. Sistem grading menunjukkan bahwa sapi PO banyak tergolong dalam grade A sebesar 58%. Sapi PO di Desa Sekaran memiliki potensi yang besar untuk dijadikan sumber bibit dan perbanyakan populasi karena telah memiliki sistem grading yang baik .Kata kunci : Desa Sekaran, digitalisasi, sapi PO, seleksi, sistem grading                                                            ABSTRACT             The development of beef cattle in Indonesia has developed in several regions by implementing animal husbandry technology and innovation through the Sekolah Peternakan Rakyat (SPR). This study aims to improve the genetic quality of PO cattles through selection based on grading systems for cattle breeding and propagation, as well as monitoring their development with a digitization program. The study was conducted with a direct interview system to farmers using a questionnaire. The research parameters included external characteristics, genealogy of cattle, and body size such as body weight and chest size. Data analysis using descriptive statistic and regression analysis. The results showed that the total of cattle in Sekaran Village was 971 with the largest population in the Ngantru approximately 94%. The cattle mating system were conducted using  artificial insemination and naturally bred were  94% and 6%, respectively.  PO cattle productivity is high with chest width measurements from 154?165 cm and body weights from 282-309 kg. The body condition score of PO cattles is in the range of 2-3 on a scale of 5. The grading system shows that many PO cattles were classified in grade A was 58%.  PO cattles in Sekaran Village have great potential to be used as a source animal genetic resources of PO breeding center.Keywords : grading system, digital, PO cattle, Sekaran village, selection
EFEK SILANG DALAM TERHADAP UKURAN TUBUH KERBAU MURRAH Syaputra, Muhammad Ary; Umar, Sayed; Gunawan, Asep
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 3 (2019): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v6i3.7602

Abstract

ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh silang dalam terhadap  ukuran tubuh kerbau murrah. Penelitian ini dilaksanakan di BPTU-HPT Siborong-borong dan di peternakan masyarakat Kabupaten Deli Serdang yaitu Desa Tanjung Garbus dan Desa Amplas Tambak Rejo. Penelitian ini menggunakan 41 ekor   ternak kerbau murrah terdiri dari 23 ekor  betina dan 18 ekor jantan (umur 1-4 bulan) dan data silsilah ternak kerbau murrah. Variabel yang diamati adalah koefisien silang dalam, ukuran populasi efektif, laju silang dalam, dan pengaruh silang dalam terhadap ukuran tubuh kerbau murrah. Hasil penelitian menunjukkan bahwa nilai koefisien silang dalam di BPTU-HPT Siborong- borong dan Deli serdang  adalah 0,218 dan 0,062. Ukuan populasi efektif kerbau murrah di Sumatera Utara sebesar 61 ekor dengan laju silang dalam per generasi sebesar 0,81%. Disimpulkan bahwa inbreeding yang terjadi di BPTU-HPT Siborong-borong dan Deli Serdang berpengaruh terhadap ukuran tubuh yaitu nilai rataan tinggi pundak, panjang badan, lingkar dada, lebar dada, dalam dada, tinggi pinggul, lebar pinggul, panjang pinggang, dan lebar pinggang.Kata kunci: kerbau murrah, koefisien silang dalam, laju silang dalam, ukuran populasi efektif, ukuran tubuh  ABSTRACTThis study was aimed to identify the effect of inbreeding on the body size of murrah buffalo. The research was done in the BPTU-HPT Siborong-borong and the community farm of Deli Serdang Regency (Tanjung Garbus village and Amplas Tambak Rejo village). The sample of murrah buffalo used 41 heads consist of females and males as many as every 23 and 18 heads (age of 1-4 months) and data of murrah buffalo pedigree. The variables measurement were inbreeding coefficient, effective population size, rate of inbreeding and the affect of inbreeding to body size of murrah buffalo. The results showed that the coeficient of the inbreeding coefficient in the BPTU-HPT Siborong?borong and Deli Serdang were 0.218 and 0.062. The effective population size of murrah buffalo in North Sumatra is 61 heads, with the rate of inbreeding per generation were 0.81 percents. It could be concluded that inbreeding that occurred in BPTU-HPT Siborong-borong and Deli Serdang the value of shoulder height, body length, chest circumference, chest width, chest depth, hip height, hip width, waist length, and waist width.Keywords: body size, effective population size, inbreeding coefficient, murrah buffalo, rate of inbreeding
PENGGUNAAN DIMETHYL SULFOXIDE SEBAGAI KRIOPROTEKTAN DALAM PEMBEKUAN SEMEN AYAM KAMPUNG (THE USE OF DIMETHYL SULFOXIDE AS CRYOPROTECTIVE AGENT FOR NATIVE CHIKEN FROZEN SEMEN) Junaedi, Junaedi; Arifiantini, Raden Iis; Sumantri, Cece; Gunawan, Asep
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aim was to determine the best dimethyl sulfoxide (DMSO) concentration for native chicken?s(Gallus gallus domesticus) frozen semen. Semen was collected from three native chickens and then evaluatedmacroscopically and microscopically. Spermatozoa that used in this study had more than 75% spermmotility and more than 25 X 108 mL in sperm concentration. Semen was divided into three equal tubesand each of them diluted with Ringer lactate egg yolk (RLEY) extender consisted of 5, 7 and 9% of DMSO.Diluted semen was added into 0.25 mL of mini straw, then equilibrated at 5 °C for two hours. The semenwas then frozen placed in liquid nitrogen vapors for10 min. Finally, the semen was put into liquid nitrogenwith a temperature of ? 196oC. Post thawing quality semen was assessed after 24 hours storage bythawing the straw in warm water (37 °C) for 30 seconds. Data were analyzed using one-way analysis ofvariance. The results demonstrated that the sperm motility and viability diluted with RLEY extenderwith 7% DMSO was better (p<0.05) than those in 5 or 9% DMSO. Semen quality decreased at every stepof freezing, especially between equilibration and thawing. Its concluded that 7% was the best DMSOconcentration for freezing of native chicken semen in Ringer lactate egg yolk extender compared to 5% and9%.
Hubungan Kualitas Layanan Perpustakaan dengan Citra BAPUSPIDA Provinsi Jawa Barat Gunawan, Asep; Saepudin, Encang; Winoto, Yunus
Khizanah al-Hikmah : Jurnal Ilmu Perpustakaan, Informasi, dan Kearsipan Vol 5, No 1 (2017): June
Publisher : Program Studi Ilmu Perpustakaan UIN Alauddin

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Abstract

Penelitian ini bertujuan untuk mengetahui sejauh mana hubungan kualitas layanan perpustakaan dengan citra perpustakaan Bapusipda Provinsi Jawa Barat. Metode penelitian ini menggunakan pendekatan kuantitatif dengan analisis survei eksplanatif. Teknik pengumpulan data dalam penelitian ini menggunakan kuesioner, wawancara, observasi, dan studi pustaka. Adapun populasi dalam penelitian ini adalah anggota perpustakaan Bapusipda yang aktif memanfaatkan perpustakaan pada bulan April tahun 2016 yaitu sebanyak 664 orang, sedangkan sampel dalam penelitian ini sebanyak 87 orang. Teknik pengambilan sampel yang digunakan dalam penelitian ini yaitu teknik simple random sampling. Hasil dari penelitian ini menujukkan bahwa terdapat hubungan positif yang signifikan antara kualitas layanan perpustakaan dengan citra perpustakaan Bapusipda Provinsi Jawa Barat, dengan koefisien korelasi (0.721), artinya hubungan kualitas layanan perpustakaan dengan citra perpustakaan Bapusipda Provinsi Jawa Barat pada taraf yang kuat.ABSTRACTThe aim of the research is to know the correlation between the service quality and the image Bapusipda Library West Java Province. This research uses a quantitative approach with survey explanative analysis. Data collection techniques used in this research are questionnaire, interview, observation, and literature study. The population in this research are 664 active members of Bapusipda who use the library in April 2016, while the sample in this research as many as 87 people. The sampling technique used in this research is simple random sampling technique. The results of this research indicate that there is a significant positive correlation between the quality of library services and the image of the Bapusipda library West Java Province, with correlation coefficients (0721), it means that the correlation between the service quality and the image Bapusipda Library West Java Province is on a strong level.
g.640T>C Polymorphism of the TGF-β2 Gene is Associated with Salmonella pullorum Resistance in Indonesian Chickens Muhsinin, Muhammad; Ulupi, Niken; Gunawan, Asep; Wibawan, I Wayan Teguh; Sumantri, Cece
ANIMAL PRODUCTION Vol 19, No 2 (2017)
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2017.19.2.597

Abstract

The objectives of this study were to identify polymorphism of transforming growth factor β2 (TGF-β2) gene associated with Salmonella pullorum resistance in Indonesian chickens. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assays were used to genotype Indonesian chickens. Analysis of polymorphism was conducted by using PopGen 3.2 software. The effect of genotype on S. pullorum resistance was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 278 chickens from 7 Indonesian chicken populations (Sentul, Merawang, Pelung, Kampung, Parent Cobb broiler, The F1 crossbreed of Kampung x Parent Cobb broiler (KB) and F2 KB x KB. The product of amplification was 284 bp. The TGF-β2| RsaI locus was polymorphic in all populations, producing two alleles (T and C) and three genotypes (TT, CT, and CC). The result from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the C allele in all populations. The χ2 analysis showed that the 6 chicken populations were deviated from Hardy-Weinberg equilibrium, with the exception of the Parent Cobb broiler chicken. The association result showed that TT genotype was significantly associated with S. pullorum resistance in Sentul chicken. Although the leukocyte concentration, leukocyte differentiation and H/L ratio in Sentul chicken with three of TGF-β2 genotypes (TT, TC, and CC) were not statistically different. In conclusion, polymorphism in the TGF-β2 chicken gene can be used as a candidate marker to increase S. pullorum immune response.
THERMOREGULATION, HAEMATOLOGICAL PROFILE AND PRODUCTIVITY OF HOLSTEIN FRIESIAN UNDER HEAT STRESS AT DIFFERENT LAND ELEVATIONS mariana, Elmy; Sumantri, Cece; Astuti, Dewi Apri; Anggraeni, Anneke; Gunawan, Asep
Buletin Peternakan Vol 43, No 1 (2019): BULETIN PETERNAKAN VOL. 43 (1) FEBRUARY 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.272 KB) | DOI: 10.21059/buletinpeternak.v43i1.37648

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The purpose of this research was to determine the effect of heat stress on thermoregulation, haematology, and productivity of Holstein Friesian (HF) dairy cows raised in different elevations. A total of 63 HF in a normal lactation period were used in this study. The research was conducted for 3 months during dry season in three different areas, which were at Pondok Ranggon (97 m.a.s.l) which categorized as a lowland, Ciawi (576 m.a.s.l) which categorized as a lower-upland, and Lembang (1241 m.a.s.l) which categorized as an upland. Observation on microclimate aspects which includes environmental temperature (Ta), relative humidity (RH) and Temperature-Humidity Index (THI) was done by recording each variable for every 2 hours starting from 08.00 to 16.00 WIB. The thermoregulation analysis was done based on the given physiological responses which consisted of the skin temperature (Ts), rectal temperature (Tr), body temperature (Tb), heart rate (Hr), respiratory rate (Rr), Heat Tolerance Coefficient (HTC), plasma cortisol level and haematological profile observation. The Ta, Rr, and THI measurements showed that in the lowland and lower-upland, the HF experienced moderate heat stress, while the HF raised in the upland area experienced less heat stress. The results showed that the dairy cows which raised in lowland had the highest HTC, Tr, Ts and Tb (P<0.05) and lowest Hr (P<0.05). All of the physiological and haematological parameters in the three study area showed a normal value.Furthermore, the erythrocyte, Hb and PVC concentration in a lowland raised HF were higher (P<0.05), while the plasma cortisol levels were not significantly different. The milk production of the observed dairy cows in different elevations was significantly different (P<0.05), with the highest milk yields, were found in the upland raised HF (13.1±3.52 kg), followed by the lower-upland (11.3±4.73 kg) and lowland (7.0±3.36 kg). In general, all of the HF raised in different land elevations was exposed to heat stress during dry seasons, even though the cows showed the ability to physiologically adapt and cope with the conditions. 
Peningkatan Protein Susu Sapi Melalui Pendekatan Seleksi Gen Pengontrol Protein Susu (Review) Asmarasari, Santiananda Arta; Sumantri, Cece; Gunawan, Asep; Taufik, Epi; Anggraeni, Anneke
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Jurnal Ilmu dan Teknologi Peternakan Tropis

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (34.169 KB)

Abstract

ABSTRAKMakalah ini ditulis dengan tujuan untuk   mengupas upaya peningkatan protein susu sapi perah  melalui pendekatan seleksi gen pengontrol protein susu. Metode penulisan makalah dilakukan dengan beberapa tahapan. Tahapan pertama, tahap persiapan, dilakukan dengan mengumpulkan dan membaca jurnal, makalah prosiding, buku yang diperoleh dari hasil dari pencarian pada internet. Dilanjutkan dengan memilih dan menentukan jurnal hasil penelitian, makalah ilmiah pada prosiding, buku terkait dengan topik yang sudah ditentukan, yaitu sesuai dengan topik makalah yang akan dibuat. Tahapan ke dua, penyusunan outline makalah yang akan ditulis, dimana pada tahapan ini untuk menentukan skema berfikir penulis terkait dengan topik yang akan di bahas. Tahapan ke tiga, mengembangkan kerangka makalah dengan menggunakan literatur ilmiah, teori, hasil penelitian yang diperoleh dari pustaka yang sudah di unduh dari internet serta sudah di baca sebelumnya. Tahapan ke empat, pemeriksaan terhadap isi makalah yang sudah ditulis, baik secara substansi maupun redaksional. Dari studi pustaka yang dilakukan disimpulkan bahwa peningkatan kualitas susu sapi mulai beralih kepada peningkatan kadar protein susu, karena alsan gizi dan ekonomi. Proses pemuliaan dalam menghasilkan ternak sapi perah dengan keunggulan memiliki kandungan protein susu yang tinggi  lebih efektif dilakukan dengan pendekatan bioteknologi molekuler. Kandungan protein susu sapi dikontrol oleh gen gen pengontrol protein susu sapi. Sehingga, dalam prosesnya, untuk menghasilkan ternak sapi perah dengan keunggulan memiliki kandungan protein susu tinggi, dapat dilakukan dengan pendekatan seleksi berdasarkan gen pengontrol protein susu, yaitu CSN1S1, CSN2, CSN1S2, dan CSN3. Setelah diketahui, gen pengontrol utama yang menyebabkan tingginya kadar protein susu sapi, maka akan dapat diketahui secara mudah ternak ternak sapi perah yang memiliki keunggulan tersebut, sehingga pelaksanaan seleksi terhadap ternak sapi perah yang akan diseleksi  dapat dilakukan dari sejak usia dini.Kata kunci: protein susu, seleksi, gen pengontrol proteinABSTRACT            This paper was written with the aim to explore efforts to increase the milk protein of dairy cows through a milk protein control gene selection approach. Paper writing method is done in several stages. The first stage, the preparation stage, is done by collecting and reading journals, proceedings papers, books obtained from the results of searching on the internet. Followed by selecting and determining the research journal, scientific papers in proceedings, books related to the topic that has been determined, which is in accordance with the topic of the paper to be made. The second stage, the preparation of the outline of the paper to be written, where at this stage to determine the authors thinking scheme related to the topic to be discussed. The third stage, develops the framework of the paper using scientific literature, theory, research results obtained from libraries that have been downloaded from the internet and read earlier. The fourth stage, an examination of the contents of the paper that has been written, both in substance and editorial. From the literature study, it was concluded that improving the quality of cows milk began to shift to an increase in milk protein levels, due to nutritional and economic conditions. The process of breeding in producing dairy cattle with the advantage of having high milk protein content is more effectively carried out by molecular biotechnology approaches. The content of cows milk protein is controlled by a cows milk protein control gene gene. So, in the process, to produce dairy cattle with the advantage of having high milk protein content, it can be done with a selection approach based on milk protein control genes, namely CSN1S1, CSN2, CSN1S2, and CSN3. Once known, the main controller gene that causes high levels of cows milk protein, it will be easily known dairy cattle that have these advantages, so that the selection of dairy cattle to be selected can be done from an early age.Keywords: milk protein, selection, protein control genes
Karakterisasi Gen KIF12 (Kinesin Familly 12) serta Hubungannya dengan Komposisi Asam Lemak pada Domba Gunawan, Asep; Tazkya, Sarah; Listyarini, Kasita; Yamin, Mohamad; Inounu, Ismeth; Sumantri, Cece
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 5, No 3 (2018): JITRO, September
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.389 KB) | DOI: 10.33772/jitro.v5i3.4978

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ABSTRAKKinesin Family 12 (KIF12) merupakan gen yang terlibat dalam mediasi kaskade antioksidan dalam sel beta sebagai sebuah target intraselular dari kelebihan asupan lemak atau lipotoksit. Penelitian ini bertujuan untuk mengidentifikasi karakterisasai gen KIF12 melalui identifikasi keragaman dan asosiasi gen KIF12 terhadap komposisi asam lemak pada domba. Sampel domba yang digunakan sebanyak 35 ekor yang terdiri dari Domba ekor Gemuk (DEG) dan Domba Ekor Tipis (DET) masing-masing 20 dan 15 ekor. Titik mutasi gen KIF12 berada pada posisi g.9617965 C>T.  Identifikasi keragaman gen KIF12 dianalisis menggunakan Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) dengan menggunakan enzim BfaI. Hasil keragaman gen KIF12 bersifat polimorfik dengan ditemukan tiga genotipe yaitu CC, CT, dan TT. Hasil Uji chi square menunjukkan bahwa lokus KIF12 berada pada keseimbangan Hardy-Weinberg (HWE) pada pada DET, hal berbeda ditunjukakan pada DEG. Titik mutasi gen KIF12 berasosiasi secara signifikan  (P <0.05) terhadap asam lemak jenuh (SFA)  asam laurik (C12:0), asam miristik (C14: 0) and asam heptadenoik (C17: 0) juga asam lemak jenuh tunggal (MUFA), asam miristoleinik (C14: 1) and asam oleik (C18: 1n9c). Gen KIF12 memiliki potensi untuk digunakan sebagai penanda seleksi terhadap komposisi asam lemak.Kata kunci:  domba, asam lemak, KIF12, PCR RFLPABSTRACTKinesin Family 12 (KIF12) is one of gene which involved in mediates an antioxidant cascade in beta cells as an intracellular target of excess fat intake or lipotoxicitys. This study was aimed to identify the polymorphism and association of KIF12 gene as candidate gene for fatty acid composition in Indonesian sheep including fat-tailed sheep and thin-tailed sheep. The sample of sheeps used 35 heads consist fat-tailed sheep and thin-tailed sheep as many as each 20 and 15 samples. Identification of polymorphism of KIF12|BfaI gene were analyzed using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP). The results of polymorphism of KIF12 gene were polymorphic for three kind of genotypes of CC, CT, and TT. The chi square revealed that the locus of KIF12 was deviated in Hardy-Weinberg equilibrium (HWE) in fat-tailed sheep, but was in HWE in thin-tailed sheep. A SNP of KIF12 was associated (P <0.05) with saturated fatty acids (SFA) including lauric acid (C12:0), myristic acid (C14: 0) and heptadecanoic acid (C17: 0) as well as monounsaturated fatty acids (MUFA), namely miristoleinic acid (C14: 1) and oleic acid (C18: 1n9c).The KIF12 gene was the potential to be used as a marker of selection for fatty acid compositions.   Keywords : sheep, fatty acids, KIF12, PCR-RFLP
SNP Discovery of Chicken Liver with Divergent Unsaturated Fatty Acid using Next Generation RNA Sequencing Gunawan, Asep; Abuzahra, Mutasem Ali M.; Listyarini, Kasita; ., Jakaria; Sumantri, Cece
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 6, No 1 (2019): JITRO, Januari
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.625 KB) | DOI: 10.33772/jitro.v6i1.5807

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ABSTRAKRNA sequencing memberikan peluang baru untuk mendeteksi variasi SNP (Single Nucleotide Polymorphism) pada perbedaan jaringan dengan perbedaan fenotipe. Tujuan dari penelitian ini adalah untuk mengkarakterisisasi penemuan SNP terbaru terkait perbedaan asam lemak tak jenuh pada ayam dengan menggunakan RNA sequencing. Sebanyak 6 sampel dipilih dari 62 sampel masing-masing 3 sampel tinggi dan 3 sampel rendah  yang merepresentasikan perbedaan fenotip yang kontras terkait asam lemak tak jenuh dianalisis dengan menggunakan RNA Sequensing. Hasil identifikasi SNP memperlihatkan  sebanyak 1208 SNP pada sampel tinggi dan rendah setelah disejajarkan dengan genom ayam Gallus gallus (GGA) v4.0. Sekitar 91% dari total SNP yang ditemukan memiliki tingkat polimorfisme yang tinggi pada 5 gen yang ditemukan terkait asam lemak yaitu gen SCD, COL6A2, CYP2J2L4, HSD17B4, dan SLC23A3. Gen SCD, HSD17B4, dan SLC23A3 memiliki jumlah titik mutasi dengan jumlah yang paling tinggi masing-masing berturut-turut  18, 13, dan 12 SNP. Tingkat level signifikan yang tinggi dan peranan dari ketiga gen tersebut yang sangat penting terkait komposisi asam lemak mengindikasikan bahwa gen SCD, HSD17B4, dan SLC23A3 merupakan tiga gen baru dan potensial untuk digunakan sebagai penanda seleksi kandungan asam lemak tak jenuh tinggi. Namun, hasil penelitian ini perlu divalidasi dan dikonfirmasi sebagai potensial kandidat gen dalam jumlah ayam yang lebih besar dan breed yang berbeda.Kata kunci: asam lemak, ayam, RNA-Seq, variasi transkriptomikABSTRACTRNA sequencing (RNA-Seq) reveals new opportunity for identification  SNP discovery in different tissues with divergent phenotype. The objective of this study was to characterize SNP profile from divergent unsaturated fatty acids using RNA-Seq. Six liver samples were selected from 62 chicken which classified 3 high and 3 low unsaturated fatty acids were analyzed using RNA-Seq. The SNP identification showed 1208 SNPs in chicken samples and a large number of those corresponded to differences between high and low chicken genome assembly Gallus gallus (GGA) v4.0. Among them, about 91% of genes had multiple polymorphisms within 5 genes (SCD, COL6A2, CYP2J2L4, HSD17B4, and SLC23A3). The SCD, HSD17B4, and SLC23A3 contained the largest number of mutations with 18, 13, and 12 SNPs respectively. Combining the significant level of SNPs and gene function related with fatty acid composition  allow us to suggest SCD, SLC23A3, HSD17B4 as the three novel and promising candidate genes for selecting unsaturated fatty acids. However, further validation is required to confirm the effect of these candidate genes in larger chicken populations.Keywords: chicken, fatty acids, RNA-Seq, transcriptome variants
FAKTOR USIA, JENIS KELAMIN, DAN JENIS CAIRAN PADA KEJADIAN FLEBITIS DI RUANG RAWAT INAP PENYAKIT DALAM Kurniawan, Rudi; Gunawan, Asep; Syafruddin, Muhammad Andri
Buletin Media Informasi Vol 13, No 2 (2017): BULETIN MEDIA INFORMASI POLTEKKES KEMENKES TASIKMALAYA
Publisher : Poltekkes Kemenkes Tasikmalaya

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Abstract

Tujuan penelitian adalah untuk mengidentifikasi gambaran faktor usia, jenis kelamin, dan jenis cairan pada kejadian flebitis di Ruang Rawat Inap Penyakit Dalam RUSD Kabupaten Ciamis. Desain penelitian menggunakan desain deskriftif. Penelitian dilaksanakan pada priode bulan Mei–Juni 2016 dengan jumlah populasi sebanyak 66 responden. Angka kejadian flebitis pasien rawat inap yang memenuhi kriteria yaitu sebanyak 32 orang (48,5%). Menurut faktor usia sebanyak 19 orang (59,4%) termasuk usia lansia (>55 tahun), menurut faktor jenis kelamin sebanyak 23 orang (71,9%) perempuan, menurut jenis cairan yaitu sebanyak 27 orang (84,4%) termasuk yang mengunakan cairan isotonik. Kejadian flebitis di Ruang Rawat Inap Penyakit Dalam RUSD Kabupaten Ciamis Priode Mei-Juni 2016 lebih cenderung terjadi pada usia lansia, berjenis kelamin perempuan, dan mengunakan cairan isotonik.