Articles

Why Mozart compositions during pregnancy should be exposed in the night. Study on apoptotic index of Rattus norvegicus offsprings’ brain neurons Maulana, Eka Nasrur; Joewono, Hermanto Tri; Widjiati, Widjiati; Purnomo, Windhu
Majalah Obstetri & Ginekologi Vol 25, No 3 (2017): December
Publisher : Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.826 KB) | DOI: 10.20473/mog.V25I32017.97-102

Abstract

Objectives: To analyze the optimal time to expose Mozart compositions(in the night/ dark) by measuring brain neural apoptotic index of Rattus norvegicus offspringsMaterials and Methods: This study used experimental random-ized post test only control group design in pregnant Rattus norvegicus. Subjects were divided into three groups at random, ie control group, 1 (1 hour Mozart exposure in dark) and 2 (1 hour Mozart in light), each comprised 8 females. After delivery, 2 heaviest Rattus norvegicus offsprings were chosen, totally 15 neonates in each group, then they were sacrificed with decapita-tion and the brain was prepared and stained using TUNEL assay method and the index of neurons cell apoptosis was calculated using microscope in 1000x magnification. This study was conducted at the Faculty of Veterinary Medicine, University of Airlangga, after conducting ethical feasibility test.Results: There were no miscarriages, congenital malformation, preterm birth and deaths in all groups. The control groups neural apoptosis index was 55.98 ± 4.12, group one 24.87±3.45 and group two 23.82±3.89. Based on statistical test result, there was no significant difference of apoptosis index between treatment group 1 and treatment group 2, with p value 0,836 but significant difference was found between control group and treatment group 1 (p=0.002) and between control group and treatment group 2 (p=0.009). In dark group we found the lower average of apoptotic index, the lowest apoptotic index, 9 of 15 had the lowest, the heaviest head weight and 4 of 15 subjects had the heaviest head weightConclusion: There was no significant difference between the pro-vision of Mozart music in dark and light on brain neuron of Rattus norvegicus offsprings although lower average in the dark group especially if the extreme value exluded. In the dark group: we found  the lower average of apoptotic index, the lowest apoptotic index, biggest proportion of the lowest index, the heaviest head and biggest proportion of heaviest head.
PEMBERIAN BUAH MERAH (PANDANUS CONOIDEUS LAM) SEBELUM DIPAPAR TIMAH HITAM MENEKAN EKSPRESI CASPASE-8 DAN JUMLAH SEL HOFBAUER MENCIT (MUS MUSCULUS) BUNTING (THE PROVISION OF RED FRUIT (PANDANUS CONOIDEUS LAM) BEFORE EXPOSED BY LEAD DECREASE EXPRESSION OF C Wahyuni, Ika; Widjiati, Widjiati; Madyawati, Sri Pantja; Rantam, Fedik Abdul
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2017.18.1.128

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The research aims to observed the provision of red fruit in placenta pregnat mice before exposed by lead. The observed case are expression of caspase-8 and number of hofbauer cells. Red fruit was expected to decrease expression of caspase-8 and number of hofbauer cells. The study design used was complete randomized design. Each treatment utilized four pregnant mice as negative control group (K-), for this group given distilled water orally during gestation 6th-15th. Positive control group (K+) given lead (0.011mg/20 g BW in 1 mL distilled water) orally during gestation 6th-15th. The treatment group namely P1, P2, and P3 treated by red fruit with different doses i.e., 8.29 mg/20 g BW, 23.98 mg/20 g BW, and 25.68 mg/20 g BW, respectively, during gestation 6th-15th, then one hour later exposed with lead. The obtained data were analyzed by Kruskal-Wallis and Mann Whitney test for calculate caspase-8 expression, ANOVA and Duncan for calculate the number of hofbauer cells. The result indicated that treatment groups which provided by red fruit of 0.8 mL/20 g BW and 0.9 mL/20 g BW showed decline expression of caspase-8 and number of hofbauer cells compare control group without admission of red fruit antioxidant and P1. Inconclusion red fruit can decrease expression of caspase-8 and number of hofbauer cell that means decrease apoptosis. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian buah merah pada plasenta mencit bunting sebelum dipapar timah hitam. Variabel yang diamati adalah ekspresi caspase-8 dan jumlah sel hofbauer. Buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer. Penelitian ini menggunakan rancangan acak lengkap (RAL). Setiap kelompok perlakuan menggunakan empat mencit bunting sebagai kelompok kontrol negatif (K-), untuk kelompok ini diberi aquades pada kebuntingan hari ke-6 sampai 15. Kelompok kontrol positif (K+) diberi timah hitam dosis 0,011 mg/20 g BB dalam 1 mL aquades selama kebuntingan hari ke-6 sampai 15. Kelompok perlakuan (P1, P2, dan P3) diberi minyak buah merah dengan dosis yang berbeda yaitu 8,29 mg/20 g BB, 23,98 mg/20 g BB, dan 25,68 mg/20 g BB. Data dianalisis dengan Kruskall-Wallis dan Mann Whitney untuk menghitung ekspresi caspase-8, sidik ragam dan uji Duncan digunakan untuk menghitung jumlah sel hofbauer. Hasil penelitian menunjukkan kelompok perlakuan yang diberi buah merah 23,98 mg/20 g BB dan 25,68 mg/20 g BB dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer dibandingkan dengan kelompok kontrol positif dan P1. Simpulan penelitian ini adalah bahwa minyak buah merah dapat menurunkan ekspresi caspase-8 dan jumlah sel hofbauer yang berarti menekan terjadinya apoptosis.
THE EFFECT OF UREA SUPLEMENTATION IN MATURATION MEDIA OF BOVINE OOCYTE IN VITRO TOWARDS EXPRESSION OF BAX, BCL-2 AND BAX/BCL-2 RATIO Kartikasari, Dhesy; Mulyati, Sri; Utama, Suzanita; srianto, Pudji; Widjiati, Widjiati; Plumeriastuti, Hani
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.15283

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This study aims to evaluate the expression of BAX, BCL-2, and BAX/BCL-2 ratio in maturation media of cow oocytes which supplemented with Urea in vitro because BAX and BCL-2 are the main regulators of apoptosis. A total of 263 oocytes from follicle aspirations originating from ovaries taken from slaughterhouses and were saturated with 3 addition of urea which was divided into three groups. The control group (P0) was control group without the addition of urea, P1 group was added with urea 20 mg/dL, while P2 group was added with urea 40 mg/dL. The results of in vitro oocyte maturation were continued with identification using immunocytochemical staining with the addition of BAX and BCL-2 antibodies. Positive results showed a brownish color on the oocyte and its cumulus. The results of this study indicated that there were significant differences (P 0.05) in BAX and BCL-2 expression, although both curves were equally increase. The increase in BCL-2 was more significant than BAX, while the BAX BCL-2 ratio did not show a significant difference (P 0.05) in whichthe curve of BAX/BCL-2 ratio was decreased. It can be concluded that the addition of urea does not affect the level of apoptosis.
ANTIOXIDANT HERBS SUPPLEMENTATION INHIBITS ENDOMETRIOSIS EXTENSION IN MICE Trisetiyono, Yuli; Widjiati, Widjiati; Hidayat, Syarief Thaufik; Pramono, Noor
Journal of Biomedicine and Translational Research Vol 5, No 2 (2019): December 2019
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v5i2.4716

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Background: Increased oxidative stress causes inflammation and increases angiogenesis. It presumed to promote the proliferation of endometriosis tissue. Kebar grass (Biophytum petersianum) and other herbs such as green tea and Cucumis melo, which contain high antioxidants, are expected to decrease oxidative stress, inflammation, angiogenesis, and reduced endometriosis implants.Objective: To investigate the effects of Kebar grass, green tea, and Cucumis melo to malondialdehyde serum, tumor necrosis factor alpha, and vascular endothelial growth factor expression, and the area of the endometriotic implants.Methods: Twenty-eight mice were divided into four groups, i.e., the first group of endometriosis mice was given Kebar grass extract; the second group was assigned green tea extract, the third group was given the combination of Cucumis melo extract?gliadin, and the last containing the untreated endometriosis mice as the control. Each treatment was given for 14 days. The data of MDA serum level, the area of the endometriotic implants, TNF-?, and VEGF expression were collected and analyzed.Results: The MDA serum levels of the groups treated with Kebar grass extract, green tea extract, and Cucumis melo extract ? gliadin were significantly lower (p=0.001) than the control group. TNF-? expression of the groups provided with each treatment also lower than the control groups (p=0.002). However, only the administration of the Cucumis melo extract?gliadin resulted in lower VEGF expression compare with the control (p=0.017). Finally, the area of the endometriotic implants of the mice models administered with each treatment was smaller than the control group (p=0.003).Conclusion: Kebar grass as well as green tea and Cucumis melo?gliadin inhibits endometriotic implants extension by decreasing MDA serum and TNF-? expression.
Red fruit oil increases trophoblast cells and decreases caspase-9 expression in placenta of lead exposed mice Sumarsono, Portia; Widjiati, Widjiati; Susilowati, Suherni
Universa Medicina Vol 35, No 2 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.110-118

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BACKGROUNDLead is able to pass through the placental barrier and interfere with fetal development. Red fruit has high antioxidant activity, due to carotenoids (pro-vitamin A), tocopherols (vitamin E) and unsaturated fatty acids. This study aims to examine the effect of red fruit oil towards placenta in pregnant mice before lead exposure.METHODSThis was a laboratory experimental post test only study, using 20 pregnant mice (Mus musculus) that were randomized into five groups. The negative control group was not exposed to lead; intervention group 0 (P0) was exposed to lead at 0.011 mg/20 g BW/day on days 6-15 of gestation. The intervention groups P1-P3 were given red fruit orally at 0.3 mL/20 g BW/day, 0.8 mL/20 g BW/day, 0.9 mL/20 g BW/day, respectively, before lead exposure. Normal trophoblast cell count and caspase-9 expression of trophoblasts were calculated. One-way ANOVA, Kruskal-Wallis and Mann Whitney tests were used to analyze the data.RESULTSThe intervention groups P2 and P3 given red fruit at 0.8 mL/20 g BW/day and 0.9 mL/20 g BW/day showed a significant increase in normal trophoblast cell count of 88.2% (p=0.024) and a decrease in caspase-9 expression of 70.8% compared with P0 to which was not given red fruit (p=0.020). CONCLUSIONRed fruit increased normal trophoblast cell count by 88.2% and decreased caspase-9 expression by 70.8% in pregnant mice before lead exposure. It should be noted that red fuit may prevent oxidative damage to cells.
Penurunan Progesterone-Induced Blocking Factor (PIBF) Sebagai Penanda Preeklampsia Faridz, Achmad Salman; Wicaksono, Budi; Dachlan, Erry Gumilar; Widjiati, Widjiati
Majalah Obstetri & Ginekologi Vol 24, No 1 (2016): Januari - April 2016
Publisher : Majalah Obstetri & Ginekologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.595 KB) | DOI: 10.20473/mog.v24i1.2756

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Tujuan: Membandingkan kadar PIBF serum dan berat plasenta pada model preeklampsia.Bahan dan Metode: Penelitian cross sectional yang dilakukan pada 16 Mus musculus bunting normal dan 16 Mus musculus bunting yang diberikan anti Qa-2 untuk membentuk suatu model preeklampsia. Kemudian dilakukan terminasi pada hari ke 16 kebuntingan dan dilakukan pemeriksaan kadar PIBF serum dengan metode ELISA serta pengukuran berat plasenta.Hasil: Didapatkan penurunan kadar PIBF serum pada Mus musculus model preeklampsia dibandingkan kontrol (33,21±29,36 ng/ml vs 105,76±59,69 ng/ml; p<0,05). Tidak ada perbedaan berat plasenta Mus musculus model preeklampsia dibandingkan kontrol (97,75±50,74 mg vs 116,09±44,45 mg; p>0,05). Tidak didapatkan hubungan antara kadar PIBF serum dengan berat plasenta.Simpulan: Penurunan kadar PIBF serum dapat dijadikan sebagai penanda kejadian preeklampsia. Penurunan kadar PIBF serum diduga berhubungan dengan kelainan plasenta pada preeklampsia.
HYALURONAN EXPRESSION ON VITRIFIED OOCYTES BEFORE AND AFTER IN VITRO MATURATION (EKSPRESI HYALURONAN PADA OOSIT YANG DIVITRIFIKASI SEBELUM DAN SESUDAH MATURASI IN VITRO) Faizah, Zakiyatul; Aswin, R. Haryanto; Lunardhi, Hamdani; Widjiati, Widjiati
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2018.19.1.71

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Oocyte vitrification is a major challenge in assisted reproductive technology. Oocyte vitrification with cumulus cells provide benefits in the process of maturation and fertilization. Vitrification leads to rapid temperature changes, therefore the decreasing in temperature could damage the cells even when the morphology was normal. Vitrification of mature oocytes is common because of its low sensitiveness towards low temperatures than immature oocytes. The aim of the research was to compare the effect of vitrification before and after in vitro maturation to the expression of hyaluronan. Maturation was operated in medium TC 50 ?L in CO2 incubators for 24 hours. Vitrification started with washing oocyte in PBS basic medium supplemented with 20% serum for 1-2 minutes, then in equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose (K1); 2) PBS + 20% serum + 0.25 M sucrose (K2); and 3).PBS + 20% serum + 0.1 M sucrose (K3). Immunocytochemical stain was performed to evaluate the hyaluronan expression. Remmele scale index (Immunoreactive score, IRS) was used to read the result. There was no differences of hyaluronan expression in oocyte and cumulus group of K1, K2 and K3 at p&lt; 0.05, statistically. We concluded that there was no difference of hyaluronan expression on oocyte and cumulus between vitrified oocyte of pre and post in vitro maturation which indicated that oocyte could be vitrified in the immature and mature state.
Nicotine supplementation blocks oocyte maturation in Rattus norvegicus Noor, Meitria Syahadatina; Bahkriansyah, H.M; Widjiati, Widjiati; Santoso, Budi
Universa Medicina Vol 32, No 2 (2013)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2013.v32.92-98

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BACKGROUNDIndonesia has the third largest tobacco consumption in the world after China and India. Nicotine as the main component of cigarette smoke has negative effects on the reproductive system, such as oocyte maturation, ovulation, and fertilization, and increasing the diploidy of oocytes. The goal of this researchwas to evaluate the effect of nicotine on oocyte maturation in Rattus norvegicus.METHODSThis was an experimental study with post test only control group design. The subjects were 40 rats selected homogenously and randomly. They were divided into a control group (receiving carboxy-methyl-cellulose sodium and 3 treatment groups (I-III) receiving nicotine subcutaneously for 7 days at dosages of 21 mg/ kgBW, 41 kg/kgBW and 84/kgBW, respectively. The observations comprised oocyte maturation stage, viz. germinal vesicle (GV), germinal vesicle breakdown (GVBD), metaphase I and metaphase II. Data were analyzed by one-way Anova with á=0.05, followed by Tukey’s HSD test.RESULTSOne-way Anova showed significant differences in oocyte  maturation in all groups. Tukey’s HSD test showed that for GV, the differing groups were control and I, control and II, I and III. For GVBD, the differing groups were control and I, I andII, I and III. For metaphase I, the differing groups were control with I, II, and III, I and II, I and III. For metaphase II, the differing groups were control versus I, II, and III, I and II, I and III.CONCLUSIONLow dose of nicotine is capable of affecting oocyte maturation in Rattus norvegicus.
Pemberian Topikal Ekstrak Daun Pepaya (Carica papaya) pada Hewan Coba Mencit (Mus musculus) Bunting Meningkatkan Kepadatan Kolagen Jaringan Vagina Djunaidi, Fauzan; Mardiyan K, Eighty; Widjiati, Widjiati
Majalah Obstetri & Ginekologi Vol 23, No 3 (2015): September - Desember 2015
Publisher : Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.42 KB) | DOI: 10.20473/mog.V23I32015.118-127

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 Tujuan: Menganalisis pengaruh pemberian gel ekstrak air daun pepaya terhadap kepadatan kolagen jaringan vagina pasca melahirkan pada hewan coba mencit saat bunting.Bahan dan Metode: Desain penelitian randomized post test only control group,dilakukan di Departemen Embriologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga, pada bulan April-Juni 2015. Terdapat empat kelompok, masing-masing kelompok terdiri dari 8 mencit bunting. Kelompok kontrol (tanpa perlakuan) dan kelompok perlakuan berupa pemberian topikal ekstrak daun pepaya konsentrasi 5%, 10% dan 15%, 1x/hari pervaginam, sejak usia kebuntingan hari ke-12 sampai melahirkan. Lama perlakuan 6-8 hari. Setelah melahirkan, mencit dikorbankan dan diperiksa kepadatan kolagen dengan pewarnaan HE. Uji statistik yang dipakai uji parametrik dan nonparametrik disertai uji komparasi gandaHasil: Kepadatan kolagen berdasarkan skor didapatkan nilai median pada kelompok I = 3,5 (3-4); kelompok II = 4 (4-4); kelompok III = 3 (3-4); kelompok IV = 4 (3-4) dengan uji Kruskal-Wallis (p=0,036) menunjukkan perbedaan signifikan, dilanjutkan uji beda tiap kelompok Mann-Whitney, hasilnya tidak didapatkan perbedaan signifikan antara kelompok kontrol dengan masing-masing kelompok perlakuan. Kepadatan kolagen berdasarkan persentase didapatkan nilai rerata pada kelompok I = 90,64%±3,35; kelompok II = 96,19%±1,34; kelompok III = 87,85%±6,69 dan kelompok IV = 92,08%±4,84. Uji Anova menunjukkan perbedaan signifikan (p=0,032), dilanjutkan uji beda LSD menunjukkan perbedaan bermakna antara kelompok I dan II (p=0,045)Simpulan: pemberian topikal ekstrak daun pepaya konsentrasi 5% pervaginam saat masa kebuntingan mencit meningkatan kepadatan kolagen jaringan vagina pasca melahirkan.
RADIATION EFFECT OF WIRELESS FIDELITY (Wi-Fi) ON OOCYTE NUMBER OF OOCYTE STIMULATION IN MICE (Mus Musculus) Nurbayatin, Anita; Widjiati, Widjiati; Primariawan, Relly Yanuari; Poernomo, Bambang; Sulistiawati, Sulistiawati; Yudiwati, Rina
Folia Medica Indonesiana Vol 53, No 3 (2017): September 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.449 KB) | DOI: 10.20473/fmi.v53i3.6440

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Infertility is a problem experienced by some women and men around the world. Most infertility problems in women is caused by impaired reproductive organs or disrupted ovulation.. One factor that causes impaired oocyte maturation is wireless fidelity (Wi-Fi) radiation which has a radio frequency field of 2.45 GHz. The radiation may increase the activity of free radical cells through the fenton reaction pathways that cause infertility because of the disrupted oocyte development. This study aimed to determine the effect of Wi-Fi radiation on the number of oocytes. This was an experimental study using control group design. The subjects were 32 female mice selected through complete random sampling and divided into two groups: control (R0) and exposure (R1). Each group consisted of 16 mice. The Wi-Fi radio frequency used was 2.5 GHz. Mice (R1) were placed closer to Wi-Fi source (± 15cm), and there were two types of laptop PCs and 3G mobile phones connected to internet placed next to mice for 15 hours/28 days. The mice underwent a simultaneous cycle with intraperitoneal injection of PMSG and HCG. Furthermore, the mice were mated with vasectomized male monomatingly to induce ovulation. The fertilization pouch in both oviducts were observed for oocyte collection. The number of oocytes was calculated using an inverted microscope. There was a difference in the number of oocytes between control and exposure group. Statistical tests were analyzed using Mann Whitney U and resulted in significant values (p value = 0.00). No oocytes count in exposure group. In other words, the group underwent anovulation. In conclusion, Wi-Fi radiation affected the number of oocyte stimulation in mice. Therefore, it was important to minimize the risk factors that trigger electromagnetic radiation on reproductive health.
Co-Authors Achmad Salman Faridz, Achmad Salman Achmad Yunus, Achmad Agung Budianto Achmad, Agung Budianto Agus Sulistyono Agustina, Widayati Alfiah Hayati Amila, Ukhti Mukminah ilmi Anwar Fauzi Ardy, Carolina Aryani, Desak Ketut Ayu Aswin, R. Haryanto Aty Widyawaruyanti Azinar, Azami D Bambang Poernomo Boedi Setiawan Brahmana Askandar Budi I Santoso, Budi I Budi Prasetyo Budi Santoso Budi Wicaksono Desky, Fitria Djianto, Anindya Marcha Angelina Eighty Mardiyan K, Eighty Erry Gumilar Dachlan, Erry Gumilar Faizah, Zakiyatul Fauzan Djunaidi, Fauzan Fedik A Rantam, Fedik A FEDIK ABDUL RANTAM Fikri, Faisal Gatut Hardianto, Gatut Glamour, Seindy H.M Bahkriansyah, H.M Hadi, Tri HS Hamdani Lunardhi Hamidah, Masyitah Hani Plumeriastuti, Hani Hapsari, Andita Hari Paraton Harijani, Nenny Hartono, Poedjo Hendrawan, Viski Fitri Hendy Hendarto Hermanto Hermanto Hermanto Tri Joewono, Hermanto Tri Hidayat, Syarief T. Hidayat, Syarief Thaufik Hidayati, Agustina Hikmah, Exma Mutatal Ika WAHYUNI INDRA YULIATI, INDRA Iwan Sahrial Hamid J, Hermanto Tri Joewono, Hermanto T Kartikasari, Dhesy Kurniawati, Eighty M Maulana, Eka Nasrur Meitria Syahadatina Noor Muninggar, Laili MY Ardianta W, MY Ngakan Made Rai Widjaja Ningrum, Nyna Puspa Noor Pramono Nurbayatin, Anita Panjaitan, Harry Mangasi Binsar Parasti, Ratriana Via Pardede, Sabrina Melisa Paulus Liben, Paulus Paulus Sugianto, Paulus Permatasari, Yulia Putri Portia Sumarsono, Portia Prianto, Adi Setyawan Pudji Srianto Purnama, Muhammad Thohawi Elziyad Rahayu, Pipit Sri Estuning Relly Yanuari Primariawan, Relly Yanuari S, Salmon Charles Salman, Achmad Sardjito, Trilas Sri Mulyati Sri Pantja Madyawati, Sri Pantja Sri Ratna Dwiningsih, Sri Ratna Suherni Susilowati Sulistiawati Sulistiawati Suzanita Utama Varianida Veterini, Varianida Wahdi, Nasrul Widiatmoko, Nur AR Widoyanti, Virki Windhu Purnomo Yudhana, Aditya Yudiwati, Rina Yuli Trisetiyono, Yuli Yuliawati, Tri Hartini