Irham Taufiqurrahman, Irham
Unknown Affiliation

Published : 28 Documents
Articles

Found 28 Documents
Search

PHYTOCHEMICAL AND CYTOTOXICITY TESTING OF RAMANIA LEAVES (Bouea macrophylla Griffith) ETHANOL EXTRACT TOWARD VERO CELLS USING MTT ASSAY METHOD (Preliminary study of adjuvant therapy materials to the preparation of the drug) Fitri, Laila; Taufiqurrahman, Irham; DH, Irnamanda
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Ramania leaves (Bouea macrophylla Griffith) are one of the local medicinal plants of South Kalimantan which is used as adjuvant therapy material. Previous research showed that ramania leaves ethanol extract showed that the extract has potential as adjuvant therapy material for anticancer, but the safety of this material is not yet known so it  needs to be tested with cytotoxicity test to know the toxic properties of the material toward normal cells in vitro. Objective: This research aims to determine the cytotoxicity properties of ramania leaves ethanol extract toward Vero cells using MTT assay method. Method: It was a true experimental research using posttest-only with control group design consist of 8 treatment groups: 31.25μg/mL, 62.5μg/mL, 125μg/mL, 250μg/mL, 500μg/mL, 1000μg/mL, 2000μg/mL and 4000μg/mL and 2 control groups which were cell control and media control. Study phases include phytochemical test, tannic acid test, cell culture, harvest and cell calculation and cytotoxicity test. The result test was read by ELISA reader, the absorbance will be calculated  by a  formula. Results: The test result showed that ramania leaves ethanol extract contained secondary metabolite such as phenol, flavonoid, steroid and terpenoid. The identification test of tannic acid compound  using TLC method (thin layer chromatography) showed the presence of tannic acid compound.The probit analysis showed IC50in 35.808μg/mL. Conclusion: Based on the research, it can be concluded that ramania leaves ethanol extract are found cytotoxic toward Vero cells after the analysis using MTT assay method (IC50<100μg/mL).
The role of ubiquinone supplementation on osteogenesis of nonvascularized autogenous bone graft Taufiqurrahman, Irham; Harijadi, Achmad; Simanjuntak, Roberto M.; D, Coen Pramono; Istiati, Istiati
Dental Journal (Majalah Kedokteran Gigi) Vol 48, No 2 (2015): (June 2015)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (888.306 KB) | DOI: 10.20473/j.djmkg.v48.i2.p59-63

Abstract

Background: Ubiquinone is one of food supplement which is known have positive effect in wound healing. However the study to evaluate the possible role of ubiquinone in bone healing in autogenous bone grafting after mandibular resection has not been studied. An in vitro study is required to evaluate whether ubiquinone or coenzyme Q-10 (CoQ10) has a positive effect on osteogenesis. Viability test of CoQ10 and a model of osteogenic-induced and hypoxic-condition mesenchymal stem cell culture were established to support the study. Purpose: The study was made to evaluate the role of ubiquinone in osteogenesis by analyzing the toxicity effect and the optimal dose of CoQ10 that might interfere in bone marrow derived mesenchymal stem cell (BM-MSC) that was dose in cell culture medium. The BM-MSC culture under hypoxia condition were also observed. Method: The toxicity and the optimum viability concentration of ubiquinone were observed using MTT assay. The osteogenic differentiation under hypoxic condition was done on BM-MSC in osteogenic medium that composed of ascorbic acid, glycerolphosphate and dexamethasone in hypoxia chamber for 21 days. Osteogenic differentiation and cellular hypoxia features were tested with immunocytochemical staining using anti-Runx2 and anti-HIF1α monoclonal antibody, respectively. Result: The maximum density value of  1.826 was found in the group of ubiquinone concentration of 75μM/ml, increasing of in concentration of ubiquinone resulted with the decrease ofoptical density of CoQ10. Statistic analysis using Anova showed with no significant difference among groups with various concentration. Immunocytochemical staining showed that Runx2 expression in 3% hypoxia group (p<0.05). Conclusion:Ubiquinone was found non toxic in its optimum dose of 75μM/ml, showed by optimum result in the expression ofRunx2 and HIF1α further study is necessary to evaluate the angiogenic and osteogenic effect ofubiquione.
THE EFFECT OF MAULI BANANA (Musa acuminata) STEM EXTRACT GEL APPLICATION WITH 37.5% CONCENTRATION ON FIBROBLAST CELL COUNT (In Vivo Study On Wound Healing Process of Male Wistar Rat (Rattus norvegicus) Buccal Mucosa) Rifasanto, Moh. Ihsan; Laillyza Apriasari, Maharani; Taufiqurrahman, Irham
Dentino Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Wound healing is the body process to improve tissue intergrity caused by wound. Mauli banana stem is a potential plant which can be used as a medicine to accelerate wound healing. Mauli banana stem extract has some contents such as tannin and saponin which has immunomodulatory properties. Previous study states that mauli banana stem extract gel with 37,5% concentration can accelerate the healing of traumatic ulcers. Purpose: To analyze the effect of mauli banana stem extract gel application with 37,5% concentration on fibroblast cell count in wound healing process of oral mucosa wistar rat on the 7th day. Material and Methods: This study was a true experimental design with posttest only control group design. It consisted of three treatment groups: a group which given mauli banana (Musa acuminata) stem extract gel with 37,5% concentration, hydroxypropyl methylcellulose gel (negative group) and patent drug gel containing Aloe vera (positive group). Result: The mean value of fibroblast cell count in wound healing process on the 7th day of the treatment group (28,57), negative group (20,14), and positive group (23). One-way Anova’s test had shown a significant difference. Post Hoc LSD test showed a significant difference between mauli banana stem extract gel with 37,5% concentration with hydroxypropyl methylcellulose gel and patent drug gel containing Aloe vera. Conclusion: The application of mauli banana stem extract gel with 37,5% concentration can increase fibroblast cell count on the 7th day in wound healing process of oral mucosa.
PENGARUH KUALITAS PELAYANAN KESEHATAN GIGI DAN MULUT TERHADAP KEPUASAN PASIEN BPJS DI LAYANAN PRIMER BANJARMASIN Aulia, Ridha; Adhani, Rosihan; Taufiqurrahman, Irham; Hatta, Isnur
Dentino Vol 2, No 1 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRACK Background: The patient satisfaction is an important thing that should be reached by every health facillty. As a spearhead of health service in National HealthF Insurance (JKN) era, public health center and dental practice were told to given a health service that coRuld be completed the patient satisfaction. BPJS health program is one of the effort to equalization of health standard for a whole Indonesian folk. It needed a good cooperation between the service provider and receiver to reaching the maximum of patient satisfaction. Purpose: To know the effect of oral health service quality to BPJS patient satisfaction in Banjarmasin primary service. Method: It used an observational analytic study with cross sectional design and the sample amounted to 400 patient consecutive sampling method. Analizing data were using product moment pearson correlation, simple linear regression and multiple linear regression. Result : The research showed that oral health service quality consisting of tangible, realiability, responsiveness, assurance and emphaty dimension had significant effect for patient (p<0,05). Conclusion : There’s a significant effect of oral health service quality to BPJS patient satisfaction in Banjarmasin primary service. Responsiveness dimension had dominant and very intense each order to patient satisfaction. A better service quality will increasing the patient satisfaction. The patient more satisfied in receiving a service can motivated them for revisit and reused the health service. Keywords: service quality, patient satisfaction, BPJS health
UJI EFEKTIVITAS EKSTRAK ETANOL DAUN RAMANIA (Bouea macrophylla Griffith) TERHADAP MORTALITAS LARVA Artemia salina Leach Aqiila, Gusti Rifda; Taufiqurrahman, Irham; Wydiamala, Erida
Dentino Vol 2, No 2 (2017)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Ramania is a typical local plant of Borneo. Ramania leaves contain secondary metabolic compounds i.e. flavonoid, saponin and triterpenoidwhich expected to be one of the alternatives for cancer treatment. Purpose: To analyzing the effectiveness of ethanol extract of ramania leaves against Artemia salina Leach larvae’s mortality using Brine Shrimp Lethality Test (BSLT). Methods: A true experimental research with post test only with control group design, using 8 treatment groups obtained by a preliminary test. Eight groups consisting of 7 extract concentrations i.e 156,25; 312,5; 625; 1250; 2500; 5000 dan 10000 mg/L and 1 control negative. Results: LC50 value by probit analysis test is 408,950 mg/L. The p-value of Kruskall-Wallis test is 0,000, there is a significant effect of ethanol extract of ramania leaves against Artemia salina Leach larvae’s mortality. The p-value of Mann-Whitney test is 0,021, there is a significant difference between negative control with all treatment groups. Conclusion: Ethanol extract of ramania leaves has effectiveness against Artemia salina Leach larvae’s mortality using Brine Shrimp Lethality Test (BSLT) with LC50 values of 408.950 mg/L.
Cytotoxicity test of binjai leaf (Mangifera caesia) ethanol extract in relation to Vero cells Dwidhanti, Fifi; Taufiqurrahman, Irham; Sukmana, Bayu Indra
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 3 (2018): (September 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (487.804 KB) | DOI: 10.20473/j.djmkg.v51.i3.p108-113

Abstract

Background: Binjai leaves (Mangifera caesia) constitute one part of a medicinal plant from South Borneo that contains potential anticancer and antioxidant flavonoids. Before using medicinal plants as adjuvant therapy material, a cytotoxicity test of a material extract needs to be conducted in order to establish the safety of natural ingredients that will be used in the production of medicinal products. Purpose: This research aimed to determine whether the ethanol extract of binjai leaves proved cytotoxic to Vero cells and determine the value of IC50 after the administering of ethanol extract of Binjai leaves by means of an MTT assay method. Methods: This research incorporated a true experimental method with posttest-only control design that consisted of ten groups. The Binjai leaf ethanol extract of varying concentrations was administered to eight groups, namely;1.25µg/mL, 62.5µg/mL, 125µg/mL, 250µg/mL, 500µg/mL, 1000µg/mL, 2000µg/mL and 4000µg/mL. The control groups consisted of two groups, one cell control group and one media control group. The cell viability percentage was calculated by an absorbent of ELISA reader. Results: The probit analysis result had an IC50 value of 2498.48µg/mL (IC50>1000µg/mL constituted a non-toxic category). Conclusion: Ethanol extract of Binjai leaves is not cytotoxic to Vero cells as shown by an assay MTT method which produced an IC50 value of 2498.48µg/mL.
Antioxidant activity test of ethyl acetate fraction of binjai (Mangifera Caesia) leaf ethanol extract Khairiah, K.; Taufiqurrahman, Irham; Putri, Deby Kania Tri
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 4 (2018): (December 2018) Article in press
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.714 KB) | DOI: 10.20473/j.djmkg.v51.i4.p164-168

Abstract

Background: Binjai (Mangifera caesia) is a herb derived from South Kalimantan possessing antioxidant properties which promote wound healing inhibiting oxidation radicals. The natural antioxidants present in binjai leaves can be extracted by fractionation. Purpose: This study aimed to analyze the antioxidant activity of ethyl acetate fraction in 96% ethanol extract of binjai leaf. Methods: The study constituted a pure experimental study incorporating a post-test design with only random sampling technique consisting of two groups, namely; an ethyl acetate fraction as the treatment group and ascorbic acid as the positive control group. The leaves were treated in accordance with the soxhlet method and subsequently fractionated to extract ethyl acetate fraction. This was used to measure antioxidant activity with DPPH radical damping method using a UV-Vis spectrophotometer. A linear regression calculation was performed with a standard curve to quantify the IC50 value, before the ethyl acetate fraction underwent a qualitative test of secondary metabolite. Results: An independent t-test indicated significant differences between groups, an average value of IC50 in ascorbic acid of 13.812 ppm with 0.996 linearity and a fraction of ethyl acetate 38.526 ppm with a linearity of 0.999. In contrast, at this linearity value ascorbic acid and ethyl fraction acetate demonstrate a very high linear connection between concentration and inhibition. A secondary metabolite test conducted on the ethyl acetate fraction produced positive results for flavonoid, tannins, and phenol. Conclusion: Based on the IC50 parameters, the fraction of ethyl acetate in 96% ethanol extract of binjai leaf produces very strong antioxidant activity in the content of the compounds in the fraction, namely: flavonoid, tannins and phenol.
FLAVONOID LEVEL ANALYSIS OF BINJAI LEAF EXTRACT (Mangifera caesia) IN ETHANOL, METHANOL, AND N-HEXANE SOLVENTS (Research report) Adham, Didy; Taufiqurrahman, Irham; Helmi, Zairin Noor
Dentino Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Many wounds can manifest in various complaints such as pain, swelling and bruising. However, almost all of these wounds can be healed faster by utilizing herbal medicine. Binjai is one of herbal medicine originated from Mangifera genus and Anarcadiaceae family which roots and stems has been proven to contain secondary metabolites. Flavonoids are one of the metabolites which have the antioxidant, antibacterial and anti-inflammatory effect. This effect may contribute in the acceleration of wound healing process especially through flavanoid compound mechanism. Flavonoids themselves are found in the form of glycosides that are more soluble in polar solvents. Thus, they can be obtained by dissolving binjai leaves in methanol and ethanol solvents. Purpose: To determine differences of total flavonoid in binjai leaf extract using ethanol and methanol solvent. Methods: This type of research is a true experimental study with post-test only control group design. The samples was comprised of 27 repetitions consisting three groups, namely 70% ethanol treatment groups and 70% methanol  treatment groups. 70% n-hexane group was used as control. Results: There were significant differences between ethanol to methanol group at p = 0.000, ethanol with n-hexane group at p = 0.000 and between methanol with n-hexane group at p = 0.000. Conclusions: There were differences of total flavonoid in binjai leaf extract using ethanol and methanol solvent.
FLAVONOID LEVEL TEST ON ETHANOL EXTRACT OF BINJAI LEAF (Mangifera Caesia) (Research report) Ansari, Ana Azizah; Taufiqurrahman, Irham; Dewi, Nurdiana
Dentino Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Binjai (Mangifera caesia) is a typical plant from South Kalimantan containing secondary metabolites such as flavonoids. Flavonoids possess antioxidant and anti-inflammatory effects that play an important role in wound healing process. Flavonoid compounds isolation from plants can be affected by many factors, one of which is the concentration of solvents. Therefore, determining solvent concentration that can optimally dissolve flavonoids is important. Purpose: The aim of this research was to discover ethanol solvent concentration that can dissolve flavonoid in Binjai leaf optimally. Methods: This study used a true experimental method with posttest-only control group design, using simple random sampling technique, consisting of seven treatment groups which are treatment group P1, P2, P3, P4, P5 and P6 using ethanol concentration of 50%, 60% , 70%, 80%, 90% and 95%, as well as a control group using n-hexane 95% in the process of maceration. Total flavonoids level is calculated using UV-Vis Spectrophotometer.  Results:  Average levels of total flavonoids contained in group P1, P2, P3, P4, P5, P6 and K were 0,025 mg; 0.055 mg; 0.112 mg; 0.068 mg; 0,049 mg; 0,139 mg and 0,026 mg respectively. One way ANOVA test showed significant differences in each group. Post Hoc LSD test revealed that there was no significant difference between 50% ethanol extract groups with 95% n-hexane extract group, whereas the other groups showed significance. Conclusion: The optimal solvent concentration to dissolve flavonoid in Binjai leaf is 95% of ethanol solvents.
ANTIOXIDANT ACTIVITY OF BINJAI LEAVES(Mangifera caesia) ETHANOL EXTRACTS (Research report) Putri, Anita Diana; Taufiqurrahman, Irham; Dewi, Nurdiana
Dentino Vol 4, No 1 (2019)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Binjai is one of Mangifera species which commonly found in South Kalimantan. Binjai leaves are known to contain flavonoids compounds, that have an effect as antioxidant that can accelerate wound healing process after tooth extraction. This study was conducted to examine the variation of  solvent concentration towards antioxidant activity of Binjai’s leaves extract using maceration method. Antioxidant activity is tested with DPPH (2,2-diphenyl-1-picrylhydrazyl) and examined using Spectrophotometer UV-Vis. Purpose: This study aims to determine solvent concentration which exxpres optimal antioxidant activity of Binjai leaves extract. Methods: This study is a true experimental with post-test only control group design. The sampling technique of Binjai leaves was determined by simple random sampling and 7 samples was opted for each treatment. Results: The study revealed that 96% ethanol extract of Binjai leaves obtained IC50 (Inhibitory Concentration) as much of 16.14 ppm (very active), 70% ethanol extract of the Binjai leaves obtained 37.94 ppm (very active), and 50% ethanol extract of Binjai leaves obtained 58.07 ppm (active). Data analysis was conducted using One Way ANOVA parametric test and LSD post hoc test which demonstrated a significant difference among 96%, 70% and 50% of ethanol  extracts of Binjai leaves with p = 0.000 (p <0.05). Conclusion: Binjai leaves extracted using 96% ethanol solvent possesses higher level of antioxidant activiy compared to 70% and 50% ethanol concentration.