Karlina Samadi, Karlina
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Inhibition effect of calcium hydroxide point and chlorhexidine point on root canal bacteria of necrosis teeth Je, Andry Leonard; Sudirman, Achmad; Samadi, Karlina
Dental Journal (Majalah Kedokteran Gigi) Vol 39, No 1 (2006): (March 2006)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.74 KB) | DOI: 10.20473/j.djmkg.v39.i1.p24-27

Abstract

Calcium Hydroxide point and Chlorhexidine point are new drugs for eliminating bacteria in the root canal. The points slowly and controly realease Calcium Hydroxide and Chlorhexidine into root canal. The purpose of the study was to determined the effectivity of Calcium hydroxide point (Calcium hydroxide plus point) and Chlorhexidine point in eleminating the root canal bacteria of nescrosis teeth. In this study 14 subjects were divided into 2 groups. The first group was treated with Calcium hydroxide point and the second was treated with Chlorhexidine poin. The bacteriological sampling were measured with spectrofotometry. The Paired T Test analysis (before and after) showed significant difference between the first and second group. The Independent T Test which analysed the effectivity of both groups had not showed significant difference. Although there was no significant difference in statistical test, the result of second group eliminate more bacteria than the first group. The present finding indicated that the use of Chlorhexidine point was better than Calcium hydroxide point in seven days period. The conclusion is Chlorhexidine point and Calcium hydroxide point as root canal medicament effectively eliminate root canal bacteria of necrosis teeth.
Differences in cytotoxicity between 5% tetracycline hydrochloride and 15% EDTA as root canal irrigant Juniarti, Devi Eka; Samadi, Karlina; Sudirman, Achmad
Dental Journal (Majalah Kedokteran Gigi) Vol 41, No 2 (2008): (June 2008)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.327 KB) | DOI: 10.20473/j.djmkg.v41.i2.p67-69

Abstract

5% tetracycline hydrochloride and 15% EDTA as a root canal irrigant have been proven to be able to remove smear layer, open dentinal tubules and have antimicrobial activity. An effective root canal irrigation solution must be able to dissolve organic and anorganic debris, lubricate endodontic instruments, disinfect microorganism and non toxic. The purpose of this laboratory experimental study was to determine cytotoxicity differences between 5% tetracycline hydrochloride and 15% EDTA. 21 samples were used and classified into 3 groups: control, 5% tetracycline hydrochloride and 15% EDTA groups. Cytotoxicity test was done using BHK21 cells. The data was analyzed using Bird and Forrester formula. It concluded that 5% tetracycline hydrochloride more toxic than 15% EDTA as a root canal irrigant.
The surface roughness difference between microhybrid and polycrystalline composites after polishing Prasetyo, Eric Priyo; Samadi, Karlina; Lunardhi, Cecilia Gerda Juliani
Dental Journal (Majalah Kedokteran Gigi) Vol 41, No 4 (2008): (December 2008)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.159 KB) | DOI: 10.20473/j.djmkg.v41.i4.p164-166

Abstract

Background: One of the success criteria for a composite resin restorative material is the surface roughness that can be achieved through polishing. Considering that there are so many types of composite resin materials on the market, including polycrystalline composites, information on this type of composite’s surface roughness is needed. Purpose: The aim of this laboratory experiment was to compare the surface roughness difference between microhybrid and polycrystalline composite after polishing. Methods: In order to obtain this, a laboratory experiment was done. Four groups of composites were produced, the first two groups consist of microhybrid composite and the second two groups consist of polycrystalline composite. Two groups with the same material were treated with two different treatments as follows: the first group was not finished (the surface is under celluloid matrix), the second group was finished and polished. After these treatments, each sample’s surface was measured using surface roughness measuring instrument and then the Results: were analyzed statistically using independent t-test (α = 0.05). Conclusion: The result showed that after polishing, the surface roughness of polycrystalline composite is lower than that of microhybrid composite.
Cytoxicity test of NaOCl and Mangosteen (Garcinia Mangostin L.) peel extract used as an irrigation solution in human periodontal ligament fibroblast cells (HPdLFc) Yuanita, Tamara; Ristyawati, Dina; Samadi, Karlina
Dental Journal (Majalah Kedokteran Gigi) Vol 51, No 3 (2018): (September 2018)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.227 KB) | DOI: 10.20473/j.djmkg.v51.i3.p133-137

Abstract

Background: Root canal irrigation is an important stage in root canal treatment as it is requires to eliminate necrotic and debris tissue as well as root canal wetting. Unfortunately, root canal irrigation can cause the material utilised to pass into the apical foramen leading to periapical complications. Consequently, the irrigation solution should have low toxicity. Sodium hypochlorite (NaOCl) is a commonly used irrigation solution since it has antibacterial properties. Moreover, NaOCl is also known to have the ability to dissolve necrotic tissue, vital pulp tissue and organic components of dentin and biofilms. Nevertheless, it can still cause damage when coming into contact with periapical tissues. On the other hand, Mangosteen peel extract (Garcinia mangostana L.), also has antibacterial activities. Hence, Mangosteen peel extract is assumed to be employable as an alternative irrigation solution. Purpose: This research aimed to reveal the toxicity levels of NaOCl and Mangosteen peel extract (Garcinia mangostin L.) used as irrigation solution in human periodontal ligament fibroblast cells (HPdLFc). Methods: HPdLFc were obtained from periapical tissues taken from one third of the first premolar teeth cultured. These cells were subsequently divided into several groups exposed to NaOCl and Mangosteen peel extract at certain concentrations. A toxicity test was then conducted using MTT assay. The results were analyzed with an Elisa reader. Cell deaths and LC50 were then calculated. Results: NaOCl became toxic at a concentration of 0.254 µl/ml or 0.025%, while Mangosteen peel extract became so at one of 2.099 ug/ml or 0.209%. Conclusion: NaOCl can be toxic at a concentration of 0.254 µl/ml or 0.025% and Mangosteen peel extract at one of 2.099 μg/ml or 0.209%.
Nonsurgical Endodontik Retreatment of Maxillary First Premolar W, Yongki Hadinata; Samadi, Karlina
DENTA Jurnal Kedokteran Gigi Vol 11, No 1 (2017)
Publisher : FKG Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v11i1.133

Abstract

Background : There are some factors can cause endodontic failure such as inadequate in cleaning or shaping step, non hermetic obturation, or poor restoration, which can cause bacteria multiply. Purpose : To report the management of endodontic failure with nonsurgical treatment. Case : 46-year-old woman came to Airlangga Dental Hospital Conservative Dentistry Department to treat her upper right tooth which show symptomatic pain in the last 2 weeks. The tooth has been treated and crowned with porcelain fused to metal about 10 years ago. Clinical examination show the presence of fistula on premolar buccal gingiva, react to percussion.? Radiographic examination show not hermetic obturation in one root canal and radiolucency in the periapical area. The diagnosis for maxillary first premolar is previously treated tooth with chronic periapical abscess.. Treatment : Crown and post was removed from the tooth, and endodontic retreatment was done. Follow up 6 months after the retreatment show no reaction to percussion, and radiographic examination show no enlargement periapical lesion. Conclusion : Nonsurgical endodontic retreatment always become the first choice to resolve endodontic failure for previously treated tooth.Keywords : endodontic failure, maxillary first premolar, nonsurgical endodontic retreatmentCorrespondence: Yongki Hadinata W., drg. PPDGS Ilmu Konservasi Gigi Fakultas Kedokteran Gigi Universitas Airlangga, Surabaya. Jl. Mayjen. Prof. Dr. Moestopo No. 47, Surabaya.
THE DIFFERENCES IN ROOT CANAL SMEAR LAYER REMOVAL BETWEEN 6,25% PINEAPPLE (ANANAS COMOCUS L. MERR.) PEEL EXTRACT AND 17% ETHYLENE DIAMINE TETRA-ACETIC ACID Pribadi, Nirawati; Samadi, Karlina; Astuti, Meliavita N. K.; Kurniawan, Hendy J.; Tandadjaja, Adelina K.; Hadi, Ratna Puspita
Dental Journal (Majalah Kedokteran Gigi) Vol 52, No 3 (2019): (September 2019)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i3.p122-125

Abstract

Background: The smear layer is attached to dentine and occludes the orifice and, consequently, must be removed in order to improve the success of inroot canal treatment. The ideal irrigation material removes both the organic and inorganic smear layers. Ethylene diamine tetra-acetic acid (EDTA) is one of the most commonly used root canal irrigation materials, but removes only inorganic smear layer. To overcome this problem, Pineapple (Ananas comosus L. Merr.) peel extract, which contains saponins, bromelain, polyphenol and flavonoid, is used during root canal irrigation. Purpose: The study aimed to analyze the difference in smear layer removal between the use of 6.25% pineapple peel extract and 17% EDTA. Methods: 27 samples of mandibular premolar teeth with straight root canals were divided randomly into three groups (n = 9) and subsequently prepared using protaper. Irrigation was performed on the control group (aquadest), group I (17% EDTA) and group II (6.25% pineapple peel extract). The samples were dried, temporarily compressed and cut horizontally from the apical to the coronal. Samples were fixed with holder before the smear layer was observed through a scanning electron microscope (SEM). The resulting data was analyzed by means of an ANOVA test. Results: The highest score of root canal hygiene was recorded by group II, followed by group I and, finally, the control group. There were significant differences between the groups (p< 0.000). Conclusion: 6.25% pineapple peel extract produces a higher smear layer removal effect than 17%EDTA on the apical 1/3 of the root canal.
THE DIFFERENCES BETWEEN MANGOSTEEN PEEL EXTRACT IRRIGANT AND NAOCL 2.5% ON ROOT CANAL CLEANLINESS Adi Santoso, Cornelia Melinda; Samadi, Karlina; Prasetyo, Eric Priyo; Wahjuningrum, Dian Agustin
Conservative Dentistry Journal Vol 10, No 1 (2020): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.001 KB) | DOI: 10.20473/cdj.v10i1.2020.40-43

Abstract

Background: Preparation, one of the stages in root canal treatment, always producesdebrisas aresultof instrumentation onroot canalwalls. Debrisconsistsoforganicand inorganicmaterials. Irrigation need to be performed in every preparation so that debrisandmicroorganisms can be removed from root canal wallsthrough flushingmechanism. NaOCl 2.5% is the most popular irrigant used in root canal treatment. However, NaOCl2.5% onlyworks onorganic tissue. Mangosteenpeel extractcontainsvariousactive compounds, such as saponin. Saponin acts assurfactantso as tolower thesurface tension and remove debris from the root canalwalls. Purpose: to compare the cleanliness of root canal walls following irrigation with NaOCl 2.5% and mangosteen peel extract. Method: Eighteen mandible premolar extracted for orthodontics necessity were used in this study. The teeth were divided into three groups, in which each group consists of six teeth. All of them were instrumented with ProTaper for Hand Use and irrigated. Group 1 used aquadest as irrigant, group 2 used NaOCl 2,5%, and group 3 used mangosteen peel extract 400 ug/ml. The roots were split longitudinally into halves and in 1/3 of apex. The surface of the canal walls were examined using scanning electron microscope. Photomicrographs were scored by three independent observers and statistically tested. Result: There were significant differences between three groups (p<0.05). Group 3, irrigated with mangosteen peel extract 400 ug/ml, had the smallest median score that was 1. Conclusion: Mangosteen peel extract is moreeffective thanNaOCl 2.5% incleaningroot canalwall from debris.
DAYA ANTIBAKTERI EKSTRAK DAUN BELIMBING WULUH (AVERRHOA BILIMBI LINN) TERHADAP BAKTERI ENTEROCOCCUS FAECALIS (ANTIBACTERIAL ACTIVITY OF AVERRHOA BILIMBI LINN LEAF EXTRACT AGAINST ENTEROCOCCUS FAECALIS) Astuti, Ranggi Hardian Nugro; Samadi, Karlina; Prasetyo, Eric Priyo
Conservative Dentistry Journal Vol 6, No 2 (2016): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.762 KB) | DOI: 10.20473/cdj.v6i2.2016.35-40

Abstract

Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml  Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony?s growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.
EFEK ANTIBIOFILM GLASS IONOMER CEMENTS DAN RESIN MODIFIED GLASS IONOMER CEMENTS TERHADAP LACTOBACILLUS ACIDOPHILUS Halim, Elsandra Novita; Samadi, Karlina; Kunarti, Sri
Conservative Dentistry Journal Vol 7, No 2 (2017): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.278 KB) | DOI: 10.20473/cdj.v7i2.2017.120-129

Abstract

Background: Risk factors for developing secondary caries are similar to those resulting in primary caries. The marginal seal of a restoration is one of the important factors predicting clinical success. The antibiofilm effect of materials used for the luting cement of oral function affects oral health. Antibiofilm properties of dental luting materials such as Glass Ionomer Cement (GIC) and Resin Modified Glass Ionomer Cement (RMGIC) may improve the restorative treatment outcome. Purpose: This experiment evaluates the antibiofilm effect of GIC and RMGIC on Lactobacillus acidophilus in vitro. Method: Lactobacillus acidophilus served as test microorganism. The quantitative microtiter plate biofilm assays were used to evaluate the antibiofilm effect of the dental luting materials on early-stage biofilm using a direct contact test (DCT) then continued by reading of Optical Density (OD) of biofilm using ELISA reader at a wavelength of 570nm. Result: GIC and RMGIC showed a decrease of OD value from negative control in all groups. The materials? elute had effect on both bacterial growth with GIC higher then RMGIC to inhibit Lactobacillus acidophilus biofilm formation. Conclusion: The antibiofilm effect of GIC more effective than RMGIC to inhibit Lactobacillus acidophilus biofilm formation. 
PERBEDAAN KETEBALAN ODONTOBLAST-LIKE CELLS SETELAH APLIKASI CAPE DAN KALSIUM HIDROKSIDA Prawitasari, Putri Galuh; Samadi, Karlina; Subiyanto, Ari
Conservative Dentistry Journal Vol 8, No 2 (2018): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.899 KB) | DOI: 10.20473/cdj.v8i2.2018.60-64

Abstract

Background : CAPE is the main component of propolis, it has several biology and fpharmocological advantages as antioxidant, antiinflammation, anticancer and as an immunomodulator. There were the research before of CAPE is capable to stimulate the production of the TGF-? 1 and collagen sintesis by the pulp tissue with many superiority compare to Ca(OH)2, recently Ca(OH)2 is  the gold standard for pulp capping treatment procedure. Objective : To determine and compare the effectiveness of CAPE and Ca(OH)2 to the thickness of odontoblast-like cells in rat pulp tissue. Methods : Maxillary first molar tooth of wistar mice was class 1 preparated until the pulp opened, then Ca(OH)2 was aplicated for 14 and 28 days, CAPE for 14 and 28 days. After application of Ca(OH)2 and propolis extract, the tooth was filled with RMGIC. Teeth were extracted on defined day and processed for histological evaluation. Result : There is a significant difference in the thickness of odontoblast-like cells after application of CAPE for 14 days with Ca(OH)2 for 14 days and CAPE for 28 days with Ca(OH)2 for 28 days. Conclusion : odontoblast-like cells after aplication of CAPE extract material is thicker than Ca(OH)2.