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Effect of oxygen hyperbaric therapy on malondialdehyde levels in saliva of periodontitis patients with type 2 diabetes mellitus Mulawarmanti, Dian; Widyastuti, Widyastuti
Dental Journal (Majalah Kedokteran Gigi) Vol 41, No 4 (2008): (December 2008)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.129 KB) | DOI: 10.20473/j.djmkg.v41.i4.p151-154

Abstract

Background: Lipid peroxidation (LPO) has implication in pathogenesis of several pathological disorders including periodontitis. Malondialdehyde (MDA) is end products of upid peroxidation. Hyperbaric Oxygen Therapy (HBOT) involves the administration of 100% oxygen under atmosphere pressure and has been used as an adjuvant therapy, while saliva is a diagnostic tool for many oral and systemic diseases. Purpose: The aim of this study was to examine the effect of HBOT on malondialdehyde in saliva to measure lipid peroxidation in periodontitis patients with type 2 Diabetes Mellitus (DM). Methods: Eight regulated type 2 DM subjects were compared to ten unregulated periodontitis patients type 2 DM (n = 18). Pre HBOT and after 10 days HBOT with 2.4 ATA dose, unstimulated whole saliva samples from study subjects were collected, centrifuged at 3000 g for 15 minutes and were then stored at −80° C until analyzed. The MDA level was determined with 2-thiobarbituric acid by a colorimetric method at 532 nm. Results: Data showed that regulated type 2 DM had lower level of MDA (3.08 ± 0.62 ug/mol) compared with unregulated periodontally- type 2 DM subjects (5.88 ± 1.04 ug/mol) (p>0.05). MDA levels were significantly lower after HBOT in regulated DM (2.30 ± 0.46 ug/mol) compared with unregulated periodontally type 2 DM (4.09 ± 0.77ug/mol) (p < 0.05). The regulated DM subjects and post HBOT showed MDA levels lower than the periodontally-unregulated group significantly. Conclusion: The saliva of periodontitis patients with unregulated type 2 DM showed more lipid peroxidation than regulated DM type 2. HBOT decreased MDA levels in regulated and unregulated type 2 DM with periodontitis.
Ekstrak daun mangrove (A.marina)mempercepat kesembuhanulkus traumatikus (Extract of mangrove leaf (A.marina) accelerates the healing of traumatic ulcer) Mendrofa, Arvian Novanolo; S, Isidora Karsini; Mulawarmanti, Dian
Journal of Dentomaxillofacial Science Vol 14, No 1 (2015): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v14i1.418

Abstract

Mangrove leaf (Avicennia marina) has a role in wound healing process, but its effect in wound healing in oral mucosa has not been researched yet. This study aimed to prove the effect of A.marina extract against traumatic ulcer and itseffective concentration in traumatic ulcer healing. The subjects of this research are 25 wistar rats that were randomized into 5 different groups; K0 control, K1 were given hyaluronic acid, P1 were given extract of A.marina10%, P2 were given extract of A.marina 20%, and P3 were given extract of A.marina 40%. After the subjects werewounded using amalgam stopper that has been heated before, they were given topical application once daily untilseven days. The ulcer diameter was measured at the second day and day 8 using caliper digital. The data, analyzedusing Kruskal-Wallis test showed that there’s difference in diameters between two groups (p&lt;0.05). The averagediameter differences of traumatic ulcer among rats are K0 = 0.5700 mm, K1 = 0.8380 mm, P1 = 0.7240 mm, P2 =0.8440 mm, and P3 = 0.9500 mm. It was concluded that A.marina extract of 10%, 20% and 40% concentrations haveeffect in the healing of traumatic ulcer, and 40% is the most effective concentration against traumatic ulcer.
Protection against periodontal destruction in diabetic condition with Sardinella longiceps fish oil: expression of matrix-metalloproteinase 8 and tissue inhibitor of metalloproteinase 1 Damaiyanti, Dian Widya; Mulawarmanti, Dian; Parisihni, Kristanti
Dental Journal (Majalah Kedokteran Gigi) Vol 52, No 1 (2019): (March 2019)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (530.323 KB) | DOI: 10.20473/j.djmkg.v52.i1.p51-56

Abstract

Background: There is strong evidence to support the claim that periodontitis may be more prevalent among diabetic individuals. Collagen degradation represents one of the key events in periodontal destructive lesions. The level of matrix metalloproteinase 8 (MMP-8) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are key to periodontal collagenolysis and associated with the severity of periodontal inflammation and disease. Host modulatory therapy has been proposed as a treatment for periodontal diseases. Sardinella longiceps (lemuru) fish oil containing polyunsaturated fatty acids (PUFAs), including omega 3 and 6, has been shown to possess therapeutic anti-inflammatory and protective properties effective against inflammatory diseases, including periodontitis. Purpose: The study aimed to examine the effect of dietary supplementation of Sardinella longiceps fish oil on protection against periodontal destruction resulting from the expression of MMP-8 and TIMP-1. Methods: Wistar rat samples are divided into four groups: a negative control group and three groups receiving Sardinella longiceps fish oil treatment (4 ml/ weight (Kg), 8 ml/ weight (Kg) and 16 ml/ weight (Kg). One week before treatment, all groups were administered with streptozotocin (STZ) 65 ml/ weight (Kg) and nicotinamide 110 ml/ weight (Kg) to induce diabetic conditions. Immunohistochemistry slides of periodontal tissues were prepared after three weeks of treatment. The expression of MMP-8 and TIMP-1 was counted using the HSCORE index, data was analyzed by means of non-parametric methods using Kruskal-Wallis, and Mann-Whitney tests. Results: Statistical analyses confirmed a significant increase in MMP-8 expression and a reduction in TIMP-1 expression in the negative control group compared to the treatment group (p<0.05). Meanwhile, the treatment group showed a significant reduction in MMP-8 expression and a marked increase in TIMP-1 expression, with the best result produced by the administering of 16 ml/ weight (Kg) Sardinella longiceps fish oil to the treatment group (p<0.05). Conclusion: Dietary supplementation of Sardinella longiceps fish oil can protect against periodontal destruction under diabetic conditions, by decreasing MMP-8 expression and increasing TIMP-1 expression.
Daya Hambat Minyak Hati Ikan Hiu Terhadap Pertumbuhan Bakteri Porphyromonas gingivalis Agustina, Felisitas Maria; Mulawarmanti, Dian; Wedarti, Yoifah Rizka
DENTA Jurnal Kedokteran Gigi Vol 9, No 2 (2015)
Publisher : FKG Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v9i2.9

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?Latar belakang : Porphyromonas gingivalis adalah bakteri paling dominan yang ditemukan pada periodontitis kronis. Antibakteri konvensional(antibiotik), terutama tetrasiklin sering digunakan untuk menunjang terapi periodontal, akan tetapi penggunaan antibiotik sering menimbulkan resisten, alergi, dan toksik.? Minyak hati ikan hiu spesies Cenctrophorus sp memiliki sifat antibakteri melalui kandungannya yaitu squalene dan squalamine, sehingga memiliki potensi untuk dikembangkan sebagai terapi ajuvan pada penyakit periodontal. Tujuan : Mengetahui daya hambat minyak hati ikan hiu terhadap pertumbuhan bakteri Porphyromonas gingivalis. Metode : Penelitian ini menggunakan rancangan penelitian post test only control group design. Subjek penelitian adalah bakteri Porphyromonas gingivalis strain ATCC 33277 sebanyak 30 sampel yang dibagi menjadi 5 kelompok. Tiga kelompok diberi minyak hati ikan hiu dengan konsentrasi 10%, 15%, dan 20%. Kontrol positif diberi tetrasiklin dan kontrol negatif diberi DMSO 1%. Daya hambat diperiksa dengan menggunakan metode difusi dan diinokulasikan pada media MH agar. Daya hambat dihitung dengan mengukur diameter zona jernih pada disk dengan menggunakan digital callipers dalam satuan milimeter. Hasil : Minyak hati ikan hiu dapat menghambat pertumbuhan bakteri Porphyromonas gingivalis pada setiap konsentrasi. Konsentrasi 10% (9,06 mm), konsentrasi 15% (11,31 mm), konsentrasi 20% (13,2 mm), sedangkan kontrol negatif (6,01 mm), dan? kontrol positif (30,09 mm). Simpulan : Minyak hati ikan hiu dapat menghambat pertumbuhan bakteri Porphyromonas gingivalis.?
Combination Administration of Stichopus hermaniiGel and Hyperbaric Oxygen Therapy the number of fibroblasts in diabetes mellitus rats with periodontitis Maulana, Faraziza; Mulawarmanti, Dian; Laihad, Fanny M.
DENTA Jurnal Kedokteran Gigi Vol 11, No 2 (2017)
Publisher : FKG Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v11i2.93

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Background: Periodontitis is the most severe complication in oral cavity to diabetes mellitus. Stichopus hermanii contain GAG, a polysaccharide which very useful in wound healing. Hyperbaric oxygen therapy is effective repairing ischemia, hypoxia, and is used as adjuvant therapy. Purpose: To determine effect of administration of Stichopus hermanii (SH) 3% gel and hyperbaric oxygen therapy 3x30 minutes with 5 minute intervals in 7 consecutive days to the number of fibroblasts in ligament periodontal diabetes mellitus rats with periodontitis. Materials and Methods: The experiment design is factorial design. Rats are inducted with STZ single dose 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis. 20 male wistar rats are divided into 5 groups. K- (control), K1 (STZ+P.Gingivalis), K2(STZ+P.Gingivalis+SH), K3(STZ+P.Gingivalis+HBO), K4(STZ+P.Gingivalis+SH+HBO). After 52 days treatment, rats is terminated to make a HPA Sample from the mandible and fibroblast is counted with 400x magnification. Result: One-way Annova here were significant differences (p&lt;0,05) in K- (32.300 ? 11.381), K1 (31.725 ? 5.590), K2 (54.225 ? 7.165), K3 (49.725 ? 10.088), K4 (72.075 ? 9.342). Conclusion: administration of Stichopus hermanii 3% gel combination and 2.4 ATA hyperbaric oxygen 3x30 minutes a day with 5 minutes intervals in 7 consecutive days can increase the number of fibroblasts in diabetes mellitus rats with periodontitis.?Keywords: Stichopus hermanii, hyperbaric oxygen, diabetes, periodontitis, fibroblasts?Correspondence: Dian Mulawarmanti, Department of Oral Biology,Faculty of Dentistry, , Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: dian.mulawarmanti@hangtuah.ac.id
Study of Hyperbaric Oxygen Theraphy and Gold sea Cucumber (Stichopus Hermanii) 3% for Collagen Density of Rat’s with Diabetes Mellitus Induced by Porphyromonas Gingivalis Bactery R, Dyah Lestarining; Mulawarmanti, Dian; Widyastuti, Widyastuti
DENTA Jurnal Kedokteran Gigi Vol 11, No 2 (2017)
Publisher : FKG Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v11i2.100

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Background: Periodontitis is complication of DM in chronic state. Periodontitis susceptibility increase threefold in patient DM compared non-DM. Debridement with SRP alone is less effective in DM. Hyperbaric Oxygen Therapy (HBO) has the effect of increasing insulin sensitivity and synthesis of collagen. Collagen is the largest extracellular matrix supporting periodontal tissue. Stchopus hermanii contains condrotin sulfate serves to inhibit the formation of mixed periodontopatogen bacteri. Purpose: To determine the therapeutic effects of ?HBO 2,4 ATA 3x3? interval 5? 7 days and stichopus hermanii against periodontal ligament collagen density in the healing process of periodontitis with DM. Materials and Methods:The experiment design is factorial design. Rats are inducted with STZ 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis, 20 male Wistar rats were divided into 4 groups. K0 (control group), K1(STZ+P. gingivalis), K2(STZ+P. gingivalis+Stichopus hermanii), K3(STZ+P. gingivalis+HBO), and K4(STZ+P.gingivalis+stichopus hermanii HBO) after treatment on day 52, all groups were sacrificed and done making preparations for the HPA of the mandible bone. Results: Data were analyzed using chi-square, Kruskal wallis, and Mann Whitney U with 95% significance level (p = 0.05). increase level of collagen significantly K1(1 ? 0.00) - K2(2?0.00), dan K1(1 ? 0.00) - K4(2.5 ? 0.289).Conclution:Therapy? HBO combination of 2.4 ATA 3 x 30 with 5 minutes air breaks for 7 days and gel Stichopus hermanii3% effective on the Densityof collagen in the periodontal ligament healing process periodontitis with DM. Keywords:OHB, Stichopus hermanii, periodontitis with DM, collagen?Correspondence: Dian Mulawarmanti, Department of Oral Biology, Faculty of Dentistry, Hang Tuah University,Arif Rahman Hakim 150, Surabaya,Phone 031-5945864, Email: dian.mulawarmanti@hangtuah.ac.id
THE EFFECTS OF SHARK LIVER OIL ON FIBROBLASTS AND COLLAGEN DENSITY IN THE PERIODONTAL LIGAMENTS OF WISTAR RATS INDUCED WITH PORPHYROMONAS GINGIVALIS Mulawarmanti, Dian; Andriani, Dwi; Damaiyanti, Dian Widya; Khoirunnisa, Farizia Putri; Juliatin, Alifati Nita
Dental Journal (Majalah Kedokteran Gigi) Vol 52, No 4 (2019): (December 2019)
Publisher : Faculty of Dental Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v52.i4.p209-214

Abstract

Background: Periodontitis is an infection in tooth-supporting tissues caused by a specific microorganism, Porphyromonas Gingivalis (PG), which can trigger collagen destruction. Generally, periodontal therapy employs a combination of mechanical (scaling root planning/SRP) and chemical (antibiotics) remedies, the latter of which can cause bacterial resistance. On the other hand, shark liver oil contains active natural ingredients such as alkylglycerols, squalene, squalamine, and omega-3, which have antibacterial and antioxidant effects. Purpose: This study aims to determine the impact of shark liver oil on fibroblasts and collagen density in the periodontal ligament of Wistar rats induced with PG. Methods: This study represents a laboratory experiment with post-test only control group design. The research subjects consisted of 35 Wistar rats divided into five groups, namely; a negative control group (K-); a positive control group with PG induction (K+); and three treatment groups induced with PG and shark liver oil once a day for seven days at varying doses of 0.2 g/gBB (P1), 0.3 g/gBB (P2), and 0.4 g/gBB (P3). Following treatment, the subjects were euthanized. The number of fibroblasts was then histologically examined with Hematoxylin Eosin (HE). Meanwhile, the collagen density was histologically analyzed with Masson?s Tricrome. Fibroblast cells were observed through a microscope at 400x magnification. Data was statistically analyzed with a one-way ANOVA and post hoc LSD. Collagen Density scoring was then performed. The results were analyzed with a non-parametric Kruskal-Wallis test (p=0.05), and subsequently with a Mann-Whitney U test (p<0.05). Results: The number of fibroblasts in the periodontal ligament areas of each group were 18.6 ± 1.21 for K-; 12 ± 1.26 for K;16.8 ± 1.72 for P1; 17.1 ± 1.94 for P2; and 23.16 ± 2.78 for P3. The results also indicated that there were significant differences between K- with K+ and P3, K+ with P1, P2, and P3, as well as P3 with P1 and P2. However, there was no significant difference between K- and P1 and P2 or P1 and P2. The results showed that collagen density in the negative control group did not significantly decrease compared to that in the positive control group in which PG was induced. Meanwhile, collagen density in all three treatment groups following doses of 0.2 g/gBB, 0.3 g/gBB, and 0.4 g/gBB being administered significantly increased compared to that in the negative control group and the positive control group subjected to PG induction. Conclusion: Shark liver oil can significantly increase fibroblast cells and collagen density in the periodontal ligament of Wistar rats induced with PG.
Efektvitas Diet Kombinasi Susu Kedelai dan Tepung Kulit Cangkang Kerang Hijau dalam Meningkatkan Panjang Lengkung Rahang Prameswari, Noengki; Razak, Arifzan; Mulawarmanti, Dian
Jurnal Material Kedokteran Gigi Vol 2 No 1 (2013): JMKG Vol 2 No 1 Maret 2013
Publisher : Ikatan Peminat Ilmu Material dan Alat Kedokteran Gigi (IPAMAGI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.891 KB)

Abstract

Calcium and magnesium are the important nutrients especially for bone and teeth growth. Cow?s milk has a high calcium, but some people cannot consume it. Soy milk as a cow?s milk alternative has a low calcium, so it might be combined with green mussel shell flour which has a high calcium. To compare diet cow?s milk, soy milk, and combination diet soy milk with green mussel shell flour to increase length of arch. Thirty two 3 weeks old male wistar rats, each weighting between 50-80 grams. This research used post test only control group design. They were randomly divided into 4 groups. K1 group was given aquadest personde 0,0225ml/gBB/day. K2 group was given cow?s milk personde 0,0225ml/gBB/day. K3 group was given soy milk personde 0,0225ml/gBB/day. K4 group was given soy milk personde 0,0225ml/gBB/day with 0,04mg/gBB green mussel shell flour. After 8 weeks, all rats were killed,took their mandible, and and measuredlength of arch by cephalometry analysis. Data was analyzed using one way Anova (p=0,05) showed significant increasing of the length of arch by cephalometry analysis compare to control group.0,00 (p&gt;0,05). Conclusion: Combination of green mussel shell flour with soy milk has proved to increase length of arch by cephalometry analysis in mandible growth.
COMPARISON OF THROMBOCYTE COUNTS DURING THE POST-ORAL ADMINISTRATION OF ASPIRIN AND THE HOLOTHURIA SCABRA ETHANOL EXTRACT IN WISTAR RATS (RATTUS NORVEGICUS) Mulawarmanti, Dian; Sari, Rima Parwati
Dental Journal (Majalah Kedokteran Gigi) Vol 53, No 2 (2020): (June 2020)
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i2.p111-114

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Background: Long bleeding time is a risk factor in dental treatment, especially in patients who consume aspirin or other antithrombotic drugs. Holothuria scabra (H. scabra) are mostly echinodermata and have been studied in Indonesia; they contain omega-3 and glycosaminoglycans, with an influence of an antithrombotic drug. Purpose: This study aimed to investigate the thrombocyte counts during the post-administration of aspirin and the H. scabra extract in Wistar rats (Rattus norvegicus). Methods: This study was true experimental with a post-test control group design. The sample consisting of 30 healthy male Wistar rats (R. norvegicus) with a bodyweight of 150?250 g was divided into three groups (n = 10). The rats in Group 1 were given sodium carboxymethyl cellulose (Na CMC). The rats in Group 2 were given aspirin, and the rats in Group 3 were given the H. scabra ethanol extract with a 25 mg/200 g dose as per their body weight (BW). Oral administration was given for seven days. The rats? blood was taken on the eighth day. The amount of thrombocyte was measured using Wright?s stain methods. The analysis of variance (ANOVA) and the Least Significant Difference (LSD) tests were conducted for data analysis (p < 0.05). Results: The thrombocyte counts (179.00 ± 10.56) in aspirin administration were lower than those in H. scabra (265.00 ± 18.54) and control groups (334.17 ± 13.9), with a significant difference between the groups (p = 0.0001; p < 0.05). Conclusion: This study indicates that the oral administration of aspirin and H. scabra decreases thrombocyte counts, whereas the administration of aspirin reduces thrombocyte counts to levels lower than those in H. scabra in Wistar rats (R. norvegicus).