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MODIFIKASI MEDIA SPIRULINA PLATENSIS SEBAGAI UPAYA PEMANFAATAN AIR LIMBAH BUDIDAYA IKAN LELE Widyantoro, Hendro; Wijayanti, Marini; Dwinanti, Sefti Heza
Jurnal Akuakultur Rawa Indonesia Vol 6, No 2 (2018): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.565 KB) | DOI: 10.36706/jari.v6i2.7159

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ABSTRACTThe purpose of this study was to determine the best fertilizer compotition that was made from technical fertilizer and waste water of cat fish farming. This experimental was conducted at Aquaculture Laboratory, Faculty of Agriculture, Sriwijaya University. It used complete randomized design (CRD) method which had five treatments and three replications, the waste water from catfish pond (P0), waste water from catfish pond + 25% technical fertilizer (P1), waste water from catfish pond + 50% technical fertilizers (P2), the waste water from catfish pond + 75% technical fertilizers (P3), the waste water from catfish pond + 100% technical fertilizers (P4). The results showed that the best maximum density of S. platensis was P3 about 3.98 g l-1, and growth rate was equal to 2.80% day-1. The most significant parameter of water quality was ammonia which decreased until 82.78%. Besides, it was more beneficial than others based on Return Cost Ratio (R/C ratio) and Benefit Cost Ratio (B/C ratio) which were 3.66 and 2.66 respectively. Keywords: Spirulina platensis, waste water pond of catfish, modified  media
PENCEGAHAN INFEKSI AEROMONAS HYDROPHILA PADA IKAN PATIN (PANGASIUS SP.) MENGGUNAKAN TEPUNG PACI-PACI (LEUCAS LAVANDULAEFOLIA) DENGAN DOSIS YANG BERBEDA Putri Anggraini, Septika; Dwi Sasanti, Ade; Wijayanti, Marini
Jurnal Akuakultur Rawa Indonesia Vol 5, No 1 (2017): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.756 KB) | DOI: 10.36706/jari.v5i1.5823

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ABTRACT The aim of this research was to know the effect of paci-paci (L. lavandulaefolia) powder addition in feed suplemented for 14 days to the prevent A. hydrophila infection Pangasius sp. The research was conducted on August-September 2016 in Laboratorium Kolam Percobaan Budidaya Perairan, Aquaculture Study Program, Agriculture Faculty, Sriwijaya University and hematocrite examination was conducted in PMI Palembang. The research based on Completely Randomized Design (CRD) with six treatments and three replications. The fish was injected by A. hydrophila with density of 1,7x108 cfu.mL-1. The Pangasius sp. has been reared for 30 days and fed with pellet enriched  with 8%, 10%, 12 % and 14% Leucas lavandulaefolia  powder. Feeding frequency was four times a day. The parameters observed on this research were prevalence, hematocrite, survival, growth and water quality. The result showed that the addition the Laucas lavandulaefolia powder in feed effect on prevalence, survival rate and patin fish growth. The treatment that produced the best prevention was the addition of 12% Leucas lavandulaefolia powder in feed which produced 28.07% prevalence, 85.00% survival rate and 1.11 cm absolute length growth. Keywords: A. hydrophila, Pangasius sp, Dosage, Leucas lavandulaefolia,
Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum TRI-PANJI, .; SUHARYANTO, .; WIJAYANTI, Marini
E-Journal Menara Perkebunan Vol 77, No 1: Juni 2009
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.392 KB) | DOI: 10.22302/iribb.jur.mp.v77i1.113

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AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas
BACTERIAL COMMUNITIES IN AQUATIC SEDIMENT FROM BUKIT DUABELAS RAINTFOREST AND OIL PALM PLANTATION AT SUMATRA INDONESIA Wijayanti, Marini; Wahyudi, Aris Tri; Yuhana, Munti; Engelhaupt, Martin; Meryandini, Anja
HAYATI Journal of Biosciences Vol. 25 No. 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1289.48 KB) | DOI: 10.4308/hjb.25.2.85

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The diversity of bacterial communities in aquatic sediment of rainforest and oil palm plantation at Sumatra was studied using pyrosequencing of 16S rRNA gene and common biodiversity indices.  Phylogenetic approach was used for revealing the community shift of bacterial phyla and genera in both areas.  Ecological approach used soil pH, total Carbon (TC), total Nitrogen (TN), available Phosphorus (AP) measurement and bacterial diversity with Shanon and Simpson indices, and bacterial richness with Chao1-ACE indices and OTUs.  Bacterial diversity and richness in aquatic sediment of forest area was higher than that ones in oil palm plantation area, although their pH, TC, and TN in both areas were not different significantly. The majority of sequences related to Proteobacteria (34.85%), Acidobacteria (32.67%), Nitrospirae (6.86%), Chloroflexi (4.31%), and Actinobacteria (4.02%) were from forest; whereas those related to Acidobacteria (46.10%), Proteobacteria (25.86%), Nitrospirae (9.20%), Chloroflexi (4.99%), and Actinobacteria (2.34%) invented from oil palm plantation. The genera of Alphaproteobacteria and Betaproteobacteria dominated in genera phylotype of bacterial 16S rRNA phylogenetic revealed both aquatic sediment of forest and oil palm plantation. The most genera in the phylogenetic tree from aquatic sediment of both areas was Burkholderia.  The bacterial community shift in aquatic sediment of forest transformation indicated higher bacterial diversity index, richness index, some of phyla and genera in aquatic sediment from forest than from oil palm plantation.
PEMELIHARAAN BENIH IKAN GURAME (OSPHRONEMUS GOURAMY) DENGAN FREKUENSI PEMBERIAN PAKAN YANG BERBEDA Wibawa, Yudha Galih; Amin, Mohamad; Wijayanti, Marini
Jurnal Akuakultur Rawa Indonesia Vol 6, No 1 (2018): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.723 KB) | DOI: 10.36706/jari.v6i1.7147

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ABSTRACT Feed management was a key factor influencing fish growth. One of the method that could be applied was the fish growth. The study aimed to determine the best of feeding frequency in rearing of gouramy fry. This research was conducted in February-March 2018 at Experimental Ponds Laboratorium of Aquculture Study Program, Faculty of Agriculture, Sriwijaya University. This study used a Randomized Completed Design (RCD) which consisted of four treatments and three replications, i.e provision of feed 2 times a day (P1), 3 times a day (P2), 4 times a day (P3), and 5 times a day (P4). The results showed that P3 (4 times a day) give the best values with the absolute weight, absolute length, feed efficiency and survival rate were 1.25 g, 1.63 cm, 90.86% and 98.89% respectively. The water quality parameters measured were temperature (26-29 oC), pH (6,5-6,7), dissolved oxygen (4,1-4,9 mg.L-1), and ammonia (0,03-0,011 mg.L-1).Keywords : Feed, Feeding frequency, Gourami fry, Growth
PENAMBAHAN SARI BUAH BELIMBING WULUH (AVERRHOA BILIMBI) PADA PAKAN UNTUK MENGOBATI IKAN LELE SANGKURIANG (CLARIAS SP.) YANG DIINFEKSI AEROMONAS HYDROPHILA Agustina, Herdia; Sasanti, Ade Dwi; Wijayanti, Marini
Jurnal Akuakultur Rawa Indonesia Vol 5, No 2 (2017): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (163.872 KB) | DOI: 10.36706/jari.v5i2.7140

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ABSTRACT Motile Aerhomonas Septicemia (MAS) is one of bacteria diseases which is often attack freshwater fish. Motile Aerhomonas Septicemia (MAS) or hemmorage septicemia caused by bacteria A. hydrophila. The aim of this reserch examine Averrhoa bilimbi juice mixed on feed in order to effect sangkuriang catfish by A. hydrophila. The research was conducted at September until October 2016 in Laboratorium Budidaya Perairan, Fakultas Pertanian, Universitas Sriwijaya and Laboraturium UPT Klinik Universitas Sriwijaya. Method of the research uses Completely Ranomized Design with six treatments and three replications. This research was conducted for 25 days of rearing. The catfish given feed mixed A. b          ilimbi juice about 150 mL.kg-1 (P1), 200 mL.kg-1 (P2), 250 mL.kg-1 (P3) dan 300 mL.kg-1 (P4) with frequency was three times a day. The parameters of this reserch is wound diameter, the percentage of cured fish, hematocrit, growth, food convertion ratio, survival rate, and chemical properties (temperature, pH, DO and ammonia). The results showed that the addition of A. bilimbi juice at P1 be obtained the percentage of cured fish was 64.47% and survival rate was 71.11%. P2 be obtained the percentage of cured fish was 68.89% and survival rate was 73.33%. P3 be obtained the percentage of cured fish was 72.53% and survival rate was 80.00%. P4 be obtained the percentage of cured fish was 95.40% and survival rate was 95.56%. P4 with dose 300 mL.kg-1 feed is the best treatment bacterial A. hydrophila infection of hematocrit was 32%, the percentage of cured fish was 95.40%, and survival rate was 95.56%. Water quality parameters during maintenance is still in a state of optimum range. Keywords: Aeromonas hydrophila, Sangkuriang catfish, Averrhoa bilimbi juice.
ISOLASI FUNGI ASAL RAWA LEBAK UNTUK BIOREMEDIASI AIR RAWA TERCEMAR BAHAN ORGANIK Saputra, Ade Bayu; Wijayanti, Marini; Jubaedah, Dade
Jurnal Akuakultur Rawa Indonesia Vol 7, No 1 (2019): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.312 KB) | DOI: 10.36706/jari.v7i1.9025

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Aquaculture sometimes only depends on water absorption without water exchange therefore the accumulation of organic material give impact on water quality in aquaculture and cause illness and death of fish. Fungi have many variations in water that can be selected by isolation and identification to maintain water quality swamp cultured. This research aims to get swamp fungi isolates as candidate bioremediator of swamp water contaminated with organic matter. Research was conducted from December 2016 to April 2017 in Laboratory of Microbiology, Department of Biology, Faculty of Science and Mathematics, Sriwijaya University and Basic Laboratory of Fisheries, Department of Aquaculture, Faculty of Agriculture, University of Sriwijaya. The methods of research started from isolation and selection of fungi, analysis of isolates capability to improve the quality of black water, and observations of swamp water quality.This research method started from isolation and selection lipolytic, cellulolytic, proteolytic fungi, the test isolates ability to improve swamp water quality and observation of water swamp quality. Monitoring  of  water  quality done  at  the sampling time, beginning and end of the research, including pH, temperature, ammonia and TDS. The results showed that the best fungi growth rate in contaminated swamp water of organic matter was 93%.day-1. Fungi isolates are able to play a role in the ammonification of bioremediation process.  
KOMBINASI LARVA LALAT BUNGA (HERMETIA ILLUCENS L.) DAN PELET UNTUK PAKAN IKAN PATIN JAMBAL (PANGASIUS DJAMBAL) Hariadi, Soikar; Irsan, Chandra; Wijayanti, Marini
Jurnal Akuakultur Rawa Indonesia Vol 2, No 2 (2014): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.407 KB) | DOI: 10.36706/jari.v2i2.2091

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ABSTRACT The objective of study was to know the effect of combination of black soldier fly larva and pellet for growth and survival rate of Pangasius djambal and also to know the optimal food proportion combination of black soldier fly larva and pellet for growth and survival rate of P. djambal. The experiment was arranged has been done for 56 days in the Jambi Freshwater Aquaculture Development Center (JFADC). Fish that used were P. djambal with average weight of 10.17 g. They were placed in aquarium size 100x50x40 cm3 with the density 10 fish/aquarium. The experiment was arranged in a completly randomized design (CRD) with five treatments. Food combination tested were as follows : P1 (100% pellet), P2 (75% pellet and 25% black soldier fly larva), P3 (50% pellet and 50% black soldier fly larva), P4 (25% pellet and 75% black soldier fly larva), P5 (100% black soldier fly larva). The measured parameters were daily growth rate, food convertion ratio, protein efficiency ratio and survival rate of P. djambal. The result of this current experiment indicated that combination of black soldier fly and pellet influenced daily growth rate, food convertion ratio, protein efficiency ratio, but not influenced survival rate of P, djambal. The optimal food combination proportion wich gave the best daily growth rate, food convertion ratio, protein efficiency ratio was 97.0-82.5% pellet and 17.5-21.0% black soldier fly larva. The survival rate of P. djambal for all treatment was 100%. Keywords: Pangasius djambal, growth, survival rate, black soldier fly larva, feed
MASKULINISASI IKAN CUPANG (BETTA SPLENDENS) MENGGUNAKAN MADU ALAMI MELALUI METODE PERENDAMAN Siregar, Annisa; Syaifudin, Mochamad; Wijayanti, Marini
Jurnal Akuakultur Rawa Indonesia Vol 6, No 2 (2018): JURNAL AKUAKULTUR RAWA INDONESIA
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (340.795 KB) | DOI: 10.36706/jari.v6i2.7158

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ABSTRACT This research aims to determine the effect of natural  honey to male percentage of betta fish by masculinization. This researchwas conducted at FisheriesBasic Laboratory,  Faculty of Agriculture, Sriwijaya University on January? February 2018. This research  usedcompletely randomized design (CRD) with 5 treatments and 3 replications. The treatment in this research was4 day of betta fish larvae  that submersed in 5 ml/L  natural honey with different immersiontime.The treatmentconsisted of P0 (without natural honey), P1 (12 hours of immersion), P2 (16 hours of immersion), P3 (20 hours of immersion),and P4 (24 hours of immersion). The observed parameters were post-immersion survival percentage, percentage of male betta fish, post- rearing survival percentage and water quality. The result showed the percentage of male betta fish was 47,50 % (P0), 66,35 % (P1), 73,51% (P2), 75,37 (P3), and 85,14 % (P4). Water quality during maintenace were temperature 27,4-28,8 oC, pH 5,3-5,8, DO 3,43-3,59 mg/L and amonia 0?001-0,002 mg/L. Treatment of P4 (24 hours of immersion) indicatedthe best treatment with larval survival percentage andmale percentage were 90.00% and 85.14% respectirely. Keywords : Betta fish (Betta splendens), masculinization,natural honey
Optimization of Spirulina platensis Culture Media as an Effort for Utilization of Pangasius Farming Waste Water Wijayanti, Marini; Jubaedah, Dade; Gofar, Nuni; Anjastari, Devi
Sriwijaya Journal of Environment Vol 3, No 3 (2018): Sustainable Resources
Publisher : Program Pascasarjana Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22135/sje.2018.3.3.108-112

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Pangasius is a fast-growing fish species that has great potential for production and export growth in Indonesia. Their farming produces a lot of organic material and ammonia which potentially make polution in freshwater body. The wastewater can be used for high value microalgal cultivation media.  The microalgae are used in various fields, one of those is Spirulina platensis, a spiral blue green algae.  This aims of this study was to determine the best composition of the technical fertilizer in the pond waste of Pangasius farming pond to obtain maximum density and know the spesific growth rate of Spirulina platensis. The research method used Completely Randomized Design (CRD) with 6 treatments (3 replication), P0 using 100% technical fertilizer without using Pangasius waste water while P1-P5 use 0% (P1), 25% (P2), 50% (P3), 75% (P4) and 100% (P5) technical fertilizer using Pangasius farming waste water. The most efficient treatment obtained 23.90 gL-1 maximum density, 6.22%.d-1 specific growth rate and 87.77% ammonia removal.