Parawansah Parawansah, Parawansah
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Journal : The Indonesian Biomedical Journal

Inhibition of Xanthine Oxidase Activity by Ethanolic Extract of Piperomia pellucida L., Acacypha indica L. and Momordica charantia L. Parawansah, Parawansah; Nuralifah, Nuralifah; Alam, Gemini; Natzir, Rosdiana
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v8i3.194

Abstract

BACKGROUND: Uric acid is a final result of purine catabolism, the enzymatic reactions in the body cells from amino acids or ribonucleotide dinucleotide. Peperomia pellucida L. (P. pellucida), Acalypha indica L. (A. indica) and Momordica charantia L. (M. charantia) are plants which have efficacy to reduce levels of uric acid excess. The aim of this research is to find out the effect of ethanol extract of P. pellucida, A. indica and M. charantia in preventing the formation of uric acid excess by inhibiting the action of the enzyme xanthine oxidase and comparing the inhibition activity of xanthine oxidase on treatments.METHODS: The study design is experimental and conducted using the enzyme xanthine oxidase, xanthine (substrate), pH 7.5 phosphate buffer, samples (P. pellucida, A. indica and M. charantia ethanol extracts) and HCL as reaction breaker. Inhibition of xanthine oxidase was determined enzymatically and unreacted xanthine was measured by UV spectrophotometer at 290 nm. The data were expressed as percent inhibition and the inhibitory concentration (IC)50 were determined using linear regresion of inhibition activity vs. concentration.RESULTS: The IC50 of P. pellucida, A. indica and M. charantia ethanol extracts in inhibiting xanthine oxidase were 19.5 ppm, 77.6 ppm and 17.8 ppm, respectively. IC50 of allopurinol was 1.99 μg/ml, and negative control (combination of enzyme and substrate) has absorbance value of 0.75026.CONCLUSION: Ethanol extract of M. charantia showed the most potent inhibition toward xanthine oxidase compared to the other two extracts.KEYWORDS: xanthine oxidase, Peperomia pellucida L., Acalypha indica L., Momordica charantia L.
Immunomodulatory Effect of Momordica charantia L. Fruit Ethanol Extract on Phagocytic Activity and Capacity of Mice Peritoneal Macrophages Parawansah, Parawansah; Nurtamin, Tomy; Mulyawati, Sufiah Asri; Nuralifah, Nuralifah; Misnaeni, Wa Ode Arlina
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.390

Abstract

BACKGROUND: The purpose of this research is to understand the secondary metabolites of Momordica charantia L. extract, as well as to disclose the potential of M. charantia extract in the phagocytic activity and capacity of peritoneummacrophages.METHODS: Examination of immunomodulatory effect was done by giving M. charantia ethanol extract on 5 treatment groups, given intra-peritoneally to mice daily. Echinacea extract as positive control and double distilled water as negative control were also given. On the 8th day, mice were infected with Staphylococcus epidermidis. After 30 minutes, peritoneum fluid was obtained to observe the activity and capacity of macrophage cells.RESULTS: The results showed significant phagocytic activity (p<0.05) at a concentration of 1,200 ppm compare to the other groups. Meanwhile the macrophage cell capacity was found statistically insignificant (p>0.05). The highest phagocytic activity was the group treated with 1,200 ppm (62%), significantly higher than other groups.CONCLUSION: The secondary metabolite content of M. charantia is alkaloids, flavonoids, tannins, saponins, and triterpenoids. The 1,200 ppm M. charantia ethanol extract is potential in inducing phagocytic activity and capacity. These results indicate that the M. charantia can be suggested as a natural immunomodulator.KEYWORDS: pare fruit, Momordica charantia L., phagocytosis, macrophage, immunomodulator