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FORMULASI SEDIAAN KRIM ANTI JERAWAT EKSTRAK ETANOL BIJI PEPAYA MUDA (CARICA PAPAYA L.) SERTA UJI AKTIVITASNYA TERHADAP BAKTERI PSEUDOMONAS AERUGINOSAATCC 27825 DAN STAPHYLOCOCCUS AUREUSATCC 25923 (ANTI ACNE CREAM FORMULATION FOR YOUNG PAPAYA SEED (CARICA PAPAYA L.) ETHANOL EXTRACT AND ACTIVITY TEST AGAINST PSEUDOMONAS AERUGINOSA ATCC 27825 AND STAPHYLOCOCCUS AUREUS ATCC 25923) Nuralifah, Nuralifah; Indradewi, Feri; Parawansah, Parawansah; Nasrun, Satriana
MEDULA Vol 7, No 1 (2019)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.139 KB) | DOI: 10.33772/medula.v7i1.11493

Abstract

Background: Young papaya seeds (Carica papaya L.) contained terpenoids, alkaloids karpain and flavonoids that have been examined has antibacterial activity. Purpose: This study aims to obtain anti-acne cream from extract of young papaya seeds (Carica papaya L.) that possess antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27825. Methods: Extract of young papaya seeds (Carica papaya L.) was obtained by maceration process using ethanol 96% and the antibacterial activity of extract conducted by well diffusion method. then the extract formulated into anti acne cream dosage and then tested to characteristic properties including pH test, dispersive power test, organoleptic test, homogeneity test, viscosity test and irritation test. Result: The results showed anti acne creams of extract young papaya seeds (Carica papaya L.) produced qualified standard and did not cause irritation. Conclusion: Anti acne cream containing extract concentration of 10% can inhibited bacteria of S. aureus ATCC 25923 with inhibition zone of 17,5 mm and P. aeruginosa ATCC 27825 with inhibition zone of 19,3 mm.Keyword: seeds of papaya (Carica papaya L.), anti acne creamLatar Belakang: Biji pepaya muda (Carica papaya L) mengandung terpenoid, alkaloid karpain dan flavonoid, yang telah diteliti memiliki aktivitas antibakteri. Tujuan: Penelitian ini bertujuan untuk memperoleh sediaan krim anti jerawat dari ekstrak biji pepaya muda (Carica papaya L) yang memiliki aktivitas anti bakteri terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aureginosa ATCC 27825. Metode: Ekstrak biji pepaya muda (Carica papaya L) diperoleh melalui proses maserasi menggunakan pelarut etanol 96%, serta uji aktivitas antibakteri ekstrak dilakukan dengan metode sumuran. Ekstrak selanjutnya diformulasikan menjadi sediaan krim anti jerawat dan dilakukan uji karakterisasi meliputi uji pH, uji daya sebar, uji organoleptik, uji homogenitas, uji viskositas dan uji iritasi. Hasil: Hasil pengamatan menunjukkan sediaan krim anti jerawat ekstrak biji pepaya muda (Carica papaya L) yang dihasilkan memenuhi syarat standar nilai pH, daya sebar dan viskositas. Hasil uji iritasi memperlihatkan formula krim tidak menimbulkan iritasi. Simpulan: Sediaan krim anti jerawat dengan konsentrasi ekstrak 10% dapat menghambat bakteri S.aureus ATCC 25923 dengan hambatan sebesar 17,5 mm dan P. aeruginosa ATCC 27825 dengan hambatan sebesar 19,3 mm.Kata Kunci: biji pepaya muda (Carica papaya L), krim anti jerawat
Inhibition of Xanthine Oxidase Activity by Ethanolic Extract of Piperomia pellucida L., Acacypha indica L. and Momordica charantia L. Parawansah, Parawansah; Nuralifah, Nuralifah; Alam, Gemini; Natzir, Rosdiana
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v8i3.194

Abstract

BACKGROUND: Uric acid is a final result of purine catabolism, the enzymatic reactions in the body cells from amino acids or ribonucleotide dinucleotide. Peperomia pellucida L. (P. pellucida), Acalypha indica L. (A. indica) and Momordica charantia L. (M. charantia) are plants which have efficacy to reduce levels of uric acid excess. The aim of this research is to find out the effect of ethanol extract of P. pellucida, A. indica and M. charantia in preventing the formation of uric acid excess by inhibiting the action of the enzyme xanthine oxidase and comparing the inhibition activity of xanthine oxidase on treatments.METHODS: The study design is experimental and conducted using the enzyme xanthine oxidase, xanthine (substrate), pH 7.5 phosphate buffer, samples (P. pellucida, A. indica and M. charantia ethanol extracts) and HCL as reaction breaker. Inhibition of xanthine oxidase was determined enzymatically and unreacted xanthine was measured by UV spectrophotometer at 290 nm. The data were expressed as percent inhibition and the inhibitory concentration (IC)50 were determined using linear regresion of inhibition activity vs. concentration.RESULTS: The IC50 of P. pellucida, A. indica and M. charantia ethanol extracts in inhibiting xanthine oxidase were 19.5 ppm, 77.6 ppm and 17.8 ppm, respectively. IC50 of allopurinol was 1.99 μg/ml, and negative control (combination of enzyme and substrate) has absorbance value of 0.75026.CONCLUSION: Ethanol extract of M. charantia showed the most potent inhibition toward xanthine oxidase compared to the other two extracts.KEYWORDS: xanthine oxidase, Peperomia pellucida L., Acalypha indica L., Momordica charantia L.
PENGARUH EKSTRAK LABU SIAM (SECHIUM EDULE (JACQ.) SWARTZ) TERHADAP WAKTU KOAGULASI PADA MUS MUSCULUS Nuralifah, Nuralifah; Wahid, Wa Ode Tika Ertia; Yusuf, Muhammad Ilyas
MEDULA Vol 2, No 2 (2015)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (344.014 KB) | DOI: 10.33772/medula.v2i2.2546

Abstract

Chayote (Sechium edule (Jacq.) Swartz) is a type of vegetable that contains most of the calcium, the calcium in the blood which stimulates the release of thromboplastin phospholipid that will catalyze changes that have not been activated prothrombin into thrombin active. The purpose of this study was to prove the existance fromthe effect of squash extracts against coagulation time in mice. This study is experimental, which is divided into 5 treatment consisted of 3 repetitions. Extracts of squash as the test material is made a concentration of 100 mg / kg, 200 mg / kg, and 300 mg / kg. Observations made by looking fibrin threads formed on the fracture capillary tube ± for 2-6 minutes after exiting the first blood. The study was conducted in the laboratory of Pharmacology Bina Husada Kendari. Statistical analysis of the results obtained from the ANOVA showed that extracts of squash has an effective effect with 15.33 seconds at a concentration of 300 mg / kg. Results of ANOVA and LSD test showed extracts of squash has an effect on coagulation time significantly.Keywords: coagulation time, extract of squash, Sechium edule (Jacq.) Swartz
UJI ANTIDIABETIK EKSTRAK BUAH PARE (MOMORDICA CHARANTIA L.) TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA MENCIT YANG DIINDUKSI STREPTOZOTOCIN Parawansah, Parawansah; Nuralifah, Nuralifah; Kholidha, Andi Noor
MEDULA Vol 5, No 1 (2017)
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.321 KB) | DOI: 10.33772/medula.v5i1.3889

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ABSTRACT Diabetes mellitus is a chronic disease in which the patient has excess glucose levels in the blood. Pare (Momordica charantia L.) is one of the plants that have efficacy in treating various diseases one of them is antidiabetes. Activity of active substances from the pare may play a role in lowering blood sugar levels. This research is done to know the effect of increasing concentration of pare extract to decrease blood glucose level in mice induced by streptozotocin.This study was experimental laboratory, the study was done by measured  the initial blood sugar level in mice, sugar content after induction of steptozotosin, and after administration of 100 mg / kgBW extract test, 250 mg / kgBW and 400 mg / kgBW with observation time for 7 days , as well as gave metformin suspension as a positive control.The results showed that the optimum value of antidiabetic activity of ethanol extract of pare fruit at a concentration of 400 mg / kgBW and had an average decrease of 122 mg / dl.Keywords: Antidiabetes, Momordica charantia L., Streptozotocin
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia Linn.) TERHADAP LARVA UDANG (Artemia salina Leach) DENGAN METODE BRINE SHRIMPLETHALITY TEST (BSLT) Parawansah, Parawansah; Nuralifah, Nuralifah; Akib, Nurlliyin; Antrie, Geong
Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017 Vol 1, No 1 (2017): Seminar Nasional Riset Kuantitatif Terapan 2017
Publisher : Prosiding Seminar Nasional Riset Kuantitatif Terapan 2017

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Abstract

Buas-buas (Premna serratifolia Linn.) belongs to the family Verbenaceae has been used as a traditional medicine. The aims of this research are to determine the compound, extract characteristics and potential toxicity (LC50) of ethanol extract of buas-buas leaves. The used method is Brine Shrimp Lethality Test (BSLT). The test consisted of six concentration treatments namely 1000, 750, 500, 250, 100 ppm and negative control with three replications. In each concentration used 10 Artemia salina leach larvae, and observations were made during 24 hours of larvae mortality. The content of phytochemical compounds from the buas-buas leaves are alkaloids, flavonoids, saponins and tannins. Characterization extract showed 62,21% contents of ethanol soluble extract, 41,5% contents of water soluble extract, 14,1% contents moisture and 4,1% contents ash. LC50 value was determined by probit analysis. The result of LC50 is 133,96 ppm. LC50<1000 ppm showed that the ethanol extract of buas-buas leaves as toxic.Kata kunci— Premna serratifolia Linn., Brine Shrimp Lethality Test (BSLT), LC50, Artemia salina Leach
Uji Toksisitas Akut Ekstrak Etanol Daun Notika (Archboldiodendron calosercium (Kobuski)) Terhadap Larva Artemia salina Leach dengan Menggunakan Metode Brine Shrimp Lethality Test (BSLT) Nuralifah, Nuralifah; Jabbar, Asriullah; Parawansah, Parawansah; Iko, Ria Agus
Pharmauho Vol 4, No 1 (2018): Pharmauho
Publisher : Pharmauho

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Abstract

Daun notika merupakan tumbuhan yang berasal dari Papua, yang tumbuh di daerah perbukitan yang telah terjadi erosi.Daun notikasecara empiris memiliki manfaat menyembuhkan penyakit malaria dan mempermudah proses persalinan. Penelitian ini bertujuanuntuk mengetahui kandungan metabolit sekunder dari daun notika, karakterisasi simplisia daun notika dan toksisitas akutmenggunakan metode BSLT terhadap larva udang Artemia salina Leach. Ekstrak daun notika diperoleh dengan menggunakanmetode maserasi dengan pelarut etanol 96%. Pengujian skrining fitokimia dilakukan dengan metode perubahan warna yangmeliputi uji alkaloid, uji flavonoid, uji terpenoid, uji tannin dan uji saponin. Pengujian karakterisasi simplisia dilakukan denganmenentukan parameter spesifik yang meliputi penetapan sari larut air dan penetapan sari larut etanol sedangkan parameter nonspesifik meliputi penetapan kadar air dan penetapan kadar abu. Pengujian toksisitas akut menggunakan metode Brine ShrimpLethality Test (BSLT) dengan menggunakan larva udang Artemia salina Leach. Hasil pengujian skrining fitokimia daun notikamenunjukkan hasil positif pada flavonoid, terpenoid, saponin dan tanin. Hasil pengujian karakterisasi simplisia pada daun notikaadalah kadar sari larut etanol adalah 50,04%, kadar sari larut air adalah 32,01%, kadar air 3,99 % dan kadar abu adalah 5,11%.Hasil pengujian toksisitas akut dari ekstrak etanol daun notika secara BSLT menunjukan bahwa daun notika tidak bersifat toksikdengan nilai LC501024,19 µg/mL sehingga dapat dimanfaatkan sebagai obat tradisional.Kata kunci: daun notika, BSLT, toksisitas akut, Artemia salina, obat tradisional
Uji Aktivitas Antibakteri Sediaan Krim Antijerawat Ekstrak Etanol Terpurifikasi Daun Sirih (Piper betle L.) Dengan Basis Vanishing Cream Terhadap Propionibacterium acne Nuralifah, Nuralifah; Armadany, Fery Indradewi; Parawansah, Parawansah; Pratiwi, Aulif
Pharmauho Vol 4, No 2 (2018): Pharmauho
Publisher : Pharmauho

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AbstrakJerawat merupakan gangguan pada kulit yang ditandai dengan adanya peradangan yang disertai penyumbatan pada saluran kelenjar minyak dalam kulit yang disebabkan oleh bakteri Propionibacterium acne. Salah satu tanaman obat yang dapat dimanfaatkan sebagai obat jerawat adalah daun sirih. Daun sirih diekstraksi dengan metode maserasi menggunakan pelarut etanol 96%. Ekstrak dipurifikasi dari klorofilnya dengan metode ekstraksi cair-cair menggunakan pelarut n-heksan. Penelitian ini bertujuan untuk mengetahui efek antibakteri terhadap ekstrak etanol daun sirih terpurifikasi dan sediaan krim dengan basis vanishing cream terhadap bakteri P. acne dengan metode sumuran. Uji stabilitas fisik sediaan krim meliputi pemeriksaan organoleptis, homogenitas, daya sebar, viskositas, pH, dan tipe krim m/a.  Hasil penelitian menunjukkan bahwa ekstrak etanol daun sirih terpurifikasi dengan konsentrasi 0,5% memiliki daya hambat yang lemah, 1% memiliki daya hambat yang sedang, 1,5% dan 2% memiliki daya hambat pertumbuhan P. acne yang kuat. Konsentrasi ekstrak dalam pembuatan krim antijerawat dengan basis vanishing cream sebesar 0,5% memiliki daya hambat yang lemah, 1% dan 1,5% memiliki daya hambat yang sedang, 2% memiliki daya hambat yang kuat sedangkan kontrol positif (asam retinoat) yang digunakan memiliki daya hambat yang kuat terhadap pertumbuhan P. acne. Krim antijerawat dengan basis vanishing cream dengan konsentrasi ekstrak 2% memiliki aktivitas antibakteri yang paling baik terhadap P. acne.Kata kunci: Antibakteri, Daun Sirih, Krim Antijerawat Tipe m/a, Propionibacterium acne.
Immunomodulatory Effect of Momordica charantia L. Fruit Ethanol Extract on Phagocytic Activity and Capacity of Mice Peritoneal Macrophages Parawansah, Parawansah; Nurtamin, Tomy; Mulyawati, Sufiah Asri; Nuralifah, Nuralifah; Misnaeni, Wa Ode Arlina
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.390

Abstract

BACKGROUND: The purpose of this research is to understand the secondary metabolites of Momordica charantia L. extract, as well as to disclose the potential of M. charantia extract in the phagocytic activity and capacity of peritoneummacrophages.METHODS: Examination of immunomodulatory effect was done by giving M. charantia ethanol extract on 5 treatment groups, given intra-peritoneally to mice daily. Echinacea extract as positive control and double distilled water as negative control were also given. On the 8th day, mice were infected with Staphylococcus epidermidis. After 30 minutes, peritoneum fluid was obtained to observe the activity and capacity of macrophage cells.RESULTS: The results showed significant phagocytic activity (p<0.05) at a concentration of 1,200 ppm compare to the other groups. Meanwhile the macrophage cell capacity was found statistically insignificant (p>0.05). The highest phagocytic activity was the group treated with 1,200 ppm (62%), significantly higher than other groups.CONCLUSION: The secondary metabolite content of M. charantia is alkaloids, flavonoids, tannins, saponins, and triterpenoids. The 1,200 ppm M. charantia ethanol extract is potential in inducing phagocytic activity and capacity. These results indicate that the M. charantia can be suggested as a natural immunomodulator.KEYWORDS: pare fruit, Momordica charantia L., phagocytosis, macrophage, immunomodulator
ANTIBACTERIAL ACTIVITIES OF KACAPIRING LEAF ETHANOL EXTRACT (GARDENIA JASMINOIDES ELLIS) ON THE BACTERIA OF STAPHYLOCOCCUS AUREUS AND PROPIONIBACTERIUM ACNES Nuralifah, Nuralifah; Armadani, Fery Indradewi; Astari, Ni Nyoman Fitri
MEDULA Vol 6, No 3 (2019): Edisi Suplemen
Publisher : Halu Oleo University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.208 KB) | DOI: 10.33772/medula.v6i3.9652

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ABSTRACTBackground and Objective(s): Infection is the process of entering pathogenic bacteria or microorganisms into the body capable of causing disease. Bacterial infections can cause skin infections such as acne. Acne is inflammation that is accompanied by blockage of the oil glands of the skin and hair (pilosebasea duct). Gardenia jasminoides Ellis is a plant that empirically can be used to treat infectious diseases caused by bacteria. This study was conducted to determine the antibacterial activity of the extracts of kacapiring leaves (Gardenia jasminoides Ellis) against Staphylococcus aureus and Propionibacterium acnes bacteria. Methods: Making extracts was done by maceration method  using  96% ethanol. The concentrations of extracts  used  were 5%, 10% and  15%,  with chloramphenicol and clindamycin as positive controls and distilled water as negative controls. Antibacterial activity was tested by the Cup-plate technique. Results: The antibacterial activity of kacapiring leaves ethanol extract to S. aureus showed that at concentrations of 5%, 10% and 15% the inhibitory power diameters values were respectively 5.58 mm, 9.3 mm and 11.41 mm while the antibacterial activity of extracts kacapiring leaves ethanol to P. acnes bacteria at concentrations of 5%, 10% and 15% had inhibitory power diameters of 6.33 mm, 9.25 mm and 11 mm respectively. Conclusion: Ethanol extract of kacapiring leaves has antibacterial activity against Staphylococcus aureus and Propionibacterium acnes bacteria. At a concentration of 5% and 10%, it shows the moderate category and at a concentration of 15% shows a strong category.Keywords: antibacterial, cup-plate technique , gardenia jasminoides
Uji Aktivitas Antihiperlipidemia Ekstrak Etanol Daun Notika (Arcboldiodendron calosericeum Kobuski) Terhadap Kadar Kolesterol Total Tikus (Rattus norvegicus) Jantan Galur Wistar Nuralifah, Nuralifah; Wahyuni, Wahyuni; Parawansah, Parawansah; Dwi Shintia, Ulan
Journal Syifa Sciences and Clinical Research Vol 2, No 1 (2020): Maret 2020
Publisher : State University of Gorontalo

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Abstract

Hyperlipidemia is an increase of lipid due to excessive fat consumption, that the intake and alteration of lipid is not balanced. Notika are plants that contain secondary metabolites in the form of flavonoids, tannins, saponins and triterpenoids which are antioxidant compounds that are thought to have the effect of reducing blood lipids. This study aims to determine the effect of ethanol extract of leaves of Notika on decreasing total cholesterol levels in rats induced by egg and propylthiouracil (PTU). This research was experimental in 6 groups, namely 4 groups given ethanol extract of leaves of Notika at a dose of 50 mg/kgBB, 100 mg/kgBB, 200 mg/kgBB, and 300 mg/kgBB, one group was given Na CMC 0.5% as a negative control, and one group was given simvastatin 10 mg as a positive control. The results showed that the ethanol extract of the leaves of Notika significantly reduced the total cholesterol level of rats with a significant value