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Antibacterial Activity of Eel (Anguilla spp.) Mucus against Salmonella typhi Nurtamin, Tomy; Nurman, Resty Yulianita; Hafizah, Indria
The Indonesian Biomedical Journal Vol 8, No 3 (2016)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v8i3.231

Abstract

BACKGROUND: Typhoid fever has become one global health problem. Typhoid fever is caused by a Gram-negative bacterium, Salmonella typhi. Eel (Anguilla spp.) is a fish which lives in the sea or in freshwater. Several previous studies have found that Anguilla spp. mucus has the ability as antibacterial against Gram-positive and negative. Although the antibacterial activity of Anguilla spp. against various pathogens had been reported, very little is known about its activity against Salmonella typhi. The aim of this study was to investigate antibacterial activity of Anguilla spp. mucus against Salmonella typhi bacteria.METHODS: Present study was an experimental in vitro test. Antibacterial activity assays were carried out by the disc diffusion method. Antibacterial activity was determined by the clear zone formed around the paper disc and minimum inhibitory concentration was determined by observing the lowest concentration which could inhibit the growth of Salmonella typhi.RESULTS: Result of the present study showed that the Anguilla spp. mucus has inhibitory effects against Salmonella typhi. Minimum inhibitory concentration from the Anguilla spp. mucus was 12.5%.CONCLUSION: Anguilla spp. mucus has antibacterial activity against the Salmonella typhi bacteria.KEYWORDS: eel fish mucus, Anguilla spp., antibacterial activity, Salmonella typhi
In Vitro Anti-Inflammatory Activities of Ethanolic Extract Elephantopus Scaber Leaves Nurtamin, Tomy; Sudayasa, I Putu; Tien, Tien
Jurnal Kedokteran dan Kesehatan Indonesia JKKI, Vol 9, No 1, (2018)
Publisher : Faculty of Medicine, Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/JKKI.Vol9.Iss1.art9

Abstract

Background: Inflammation is a protective physiological response to tissue injury that can be caused by harmful stimuli. If the inflammatory process is prolonged and cannot restore to homeostatic conditions, this may lead to pathological effects that can damage cells and cause various diseases. Elephantopus scaber is a plant that can easily be found in Indonesia. Elephantopus scaber is a type of plant that is often used as a traditional medicines. Several studies have shown that the compound bioactive content contained in plants has enormous potential as alternative medicine.Objective: This present study was to investigate the anti-inflammatory activity of ethanolic extract of Elephantopus scabe leaves.Methods: The Elephantopus scaber leaves were extracted using ethanol solvent into different concentration (50 mg/mL, 100 mg/mL, and 120 mg/mL). Diclofenac sodium was used as the standard. Anti-inflammatory assays were performed by the human red blood cell (HRBC) membrane stabilization method and heat-induced hemolysis method. Phytochemical screening that used in the present study was a conventional method.Results: Phytochemical screening showed the presence of flavonoids, tannins, and saponins. In the present study, ethanolic extract of Elephantopus scaber leaves has anti inflammatory activity by protecting the stability of red blood cell membrane. The highest protection capability possessed by the ethanolic extract of Elephantopus scaber leaves in both human red blood cell (HRBC) membrane stabilization method and heatinduced hemolysis method was at a concentration of 100 mg/mL.Conclusion: The ethanolic extract of Elephantophus scaber has antiinflammatory activities by in vitro assays.
Hubungan Lingkungan Rumah dengan Kejadian Infeksi Saluran Pernapasan Akut (ISPA) pada Masyarakat Pesisir Kelurahan Lapulu Kecamatan Abeli Tahun 2014 Yusuf, Milawati; Sudayasa, I Putu; Nurtamin, Tomy
Medula Vol 3, No 2 (2016)
Publisher : Medula

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.265 KB)

Abstract

Acute Respiratory Infection (ARI) is a major cause of morbidity and mortality in children. ARI in Indonesia was ranked 6th in the world, reaching 6 million cases per year. One of factors that influence incidence of ARI is house environment. This study aimed to determine the relationship  bertween house environment with acute respiratory infections (ARI) incidence in coastal communities in Lapulu Village, Subdistrict of Abeli 2014. This study is analytical observational with cross sectional study design. The sample size was 88 samples by applying proportional sampling technique. The independent variable were household crowding, natural ventilation, natural lighting, humidity, floor type, wall type and location of the kitchen. The data were analyzed by using chi-square test. The result at  significance level α = 0,05 showed that there is relationship between  household crowding (p-value = 0,000, CC = 0,415), natural ventilation (p-value=0,000, CC=0,394) , natural lighting (p-value= 0,001, CC= 0,330) , and humidity (p-value=0,015, CC= 0,250) with ARI incidence. Meanwhile, floor type (p-value=0,880, CC= 0,016), wall type  (p-value=0,084, CC= 0,181), and location of the kitchen (p-value=0,582, CC= 0,059) does not show a relationship with ARI incidence. The conclusion that there are relationship between household crowding, natural ventilation, natural lighting and humidity with ARI incidence. In contrast, floor type, wall type, and location of the kitchen are not related with ARI incidence. Keywords : Acute Respiratory Infection (ARI), house environment, coastal communities
Hubungan Depresi dengan Kemampuan Dalam Aktivitas Dasar Sehari-Hari Pada Lanjut Usia di Panti Sosial Tresna Werdha Minaula Kendari Pamungkas, Dimas Aji; Nurtamin, Tomy; Junuda, Junuda
Medula Vol 3, No 2 (2016)
Publisher : Medula

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.597 KB)

Abstract

According to PPDGJ-III depression is a mental disorder that has three major symptoms of depressive affect, loss of interest and easily tired state, and has seven other symptoms such as reduced concentration, reduced confidence, feelng of guilty, pessimistic, suicidal ideas, sleep disturbance and loss of appetite. These problem could be acute or chronic and lead impaired ability of individuals in everyday activities. Activity Daily Living is work activities of daily routine. Activity Daily Living is a staple for self-care activities. These study aims to determine the relationship between depression and activity daily living the elderly in Panti Sosial Tresna Werdha “Minaula” Kendari. These study was an observational analytic study with cross sectional design. The population from  elderly in Panti Sosial Tresna Werdha "Minaula" Kendari. Sample in these study consist of  95 respondent. Elderly male cosist of 35 respondents and female 36 respondents. The sampling method is total sampling. The study instrument used questionnaires Beck Depression Inventory (BDI) to assess depression in the elderly. Assessment Activity Daily Living (ADL) using the Barthel Index questionnaire. Data were analyzed by Spearman test. Data was cosidered significant at p value ≤ 0,05. The Results of these study  that 71 respondents was mild depression (43.7%), no depression (33.8%) and moderate depression (22.5%).  The Respondent who can perform the Activity Daily Living consist of independently (42.3%), mild dependence (38.0%), moderate dependence (9.9%), heavy dependence (7.0%) and total dependence (2.8%). There were a significant relationship between depression on Activity Daily Living (p = 0.000) with negative correlation direction or opposite direction (r = -0.500). The conclusions of these study indicate that there were relationship of depression of activity daily living the elderly in Panti Sosial Tresna Werdha “Minaula” Kendari.Keywords: Activity Daily Living, Depression, Elderly
Immunomodulatory Effect of Momordica charantia L. Fruit Ethanol Extract on Phagocytic Activity and Capacity of Mice Peritoneal Macrophages Parawansah, Parawansah; Nurtamin, Tomy; Mulyawati, Sufiah Asri; Nuralifah, Nuralifah; Misnaeni, Wa Ode Arlina
The Indonesian Biomedical Journal Vol 10, No 2 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i2.390

Abstract

BACKGROUND: The purpose of this research is to understand the secondary metabolites of Momordica charantia L. extract, as well as to disclose the potential of M. charantia extract in the phagocytic activity and capacity of peritoneummacrophages.METHODS: Examination of immunomodulatory effect was done by giving M. charantia ethanol extract on 5 treatment groups, given intra-peritoneally to mice daily. Echinacea extract as positive control and double distilled water as negative control were also given. On the 8th day, mice were infected with Staphylococcus epidermidis. After 30 minutes, peritoneum fluid was obtained to observe the activity and capacity of macrophage cells.RESULTS: The results showed significant phagocytic activity (p<0.05) at a concentration of 1,200 ppm compare to the other groups. Meanwhile the macrophage cell capacity was found statistically insignificant (p>0.05). The highest phagocytic activity was the group treated with 1,200 ppm (62%), significantly higher than other groups.CONCLUSION: The secondary metabolite content of M. charantia is alkaloids, flavonoids, tannins, saponins, and triterpenoids. The 1,200 ppm M. charantia ethanol extract is potential in inducing phagocytic activity and capacity. These results indicate that the M. charantia can be suggested as a natural immunomodulator.KEYWORDS: pare fruit, Momordica charantia L., phagocytosis, macrophage, immunomodulator