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HUBUNGAN PANJANG DAN BOBOT IKAN NILA LOKAL, BEST F5 DAN F6 DI PANGKEP, SULAWESI SELATAN PADA UMUR 60 HARI PEMELIHARAAN Kusmini, Irin Iriana; Gustiano, Rudhy; Putri, Fera Permata
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.685

Abstract

Best tilapia is breed fish developed by Research Center and Development of Freshwater Aquaculture (BPPBAT) Bogor that has been launched since 2008. Best tilapia is the third generation of gift tilapia. In order to obtain a better growth performance, BPPBAT continued the process of breeding, and to date we have obtained the sixth generations of Best tilapia. This study was aimed to determine growth of tilapia through observation of the relationship between the length and weight of local tilapia, F5 and F6 BEST which were maintained in brackish waters in the Pangkep area, South Sulawesi. Fish samples were taken at random, 30 fishes were taken from each population for length and weight measurement. Calculation of the length-weight of fish in the Pangkep area obtained regression coefficient (b) for local fish 2.4361; BEST F5 3.1077 and F6 3.0522. These values indicated that the local tilapia was negatively allometric while BEST was positively allometric, with local fish condition factor ranged between 0.471-1.334, F5; 0.468-1.479 and 0.498-1.534 F6 respectively. This results has indicating the normal condition of the fish. The length-weight relationship of fish had a value of determinant (R 2) ranged from 0.7829-0.9182, and indicated fish variations influenced by other variables was rather small and has suggested close relationship between length and weight.
PERTUMBUHAN DAN PERKEMBANGAN GONAD IKAN BAUNG (HEMIBAGRUS NEMURUS) KETURUNAN G-1, G-2, DAN G-3 Kusmini, Irin Iriana; Kristanto, Anang Hari; Widiyati, Ani; Putri, Fera Permata
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.861 KB) | DOI: 10.15578/jra.14.4.2019.201-211

Abstract

Ikan baung (Hemibagrus nemurus) telah berhasil didomestikasi oleh Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan, Bogor. Hasil penelitian domestikasi, populasi Cirata memiliki fekunditas dan pertumbuhan yang lebih bagus, populasi ini dikembangkan hingga tiga generasi (G1, G2 dan G3) namun, ada informasi terbatas mengenai perbandingan antara perkembangan gonad dari berbagai generasi ikan. Tujuan penelitian ini adalah untuk mengukur pertumbuhan dan mengamati perkembangan gonad generasi pertama (G-1), kedua (G-2), dan ketiga (G-3). Parameter yang diukur dan diamati meliputi pengukuran panjang, berat, laju pertumbuhan spesifik, morfologi dan berat gonad, diameter oosit, dan indeks gonad somatik (IGS). Pengambilan sampel dilakukan setiap dua bulan dengan mengumpulkan sepuluh sampel ikan dari setiap generasi. Jaringan gonad disiapkan secara histologis dan diwarnai larutan asetokarmin sebagai zat pewarna. Pengamatan menggunakan mikroskop dengan perbesaran 4 x 10. Hasil penelitian menunjukkan bahwa pada usia 222 hari, gonad jantan dan betina secara fisik masih dalam bentuk benang halus. Namun, karakteristik sekunder pada ikan jantan seperti papila sudah mulai tampak meski tidak terlalu jelas. Pada usia 283 hari, ukuran oosit berkisar antara 0.025-0.05 mm. Pada usia 345 hari, telur mulai terlihat dengan ukuran oosit G-1, G-2, dan G-3 masing-masing berkisar antara 0.59 ± 0.24, 0.39 ± 0.15, dan 0.48 ± 0.15 mm. Setelah usia 406 hari, perkembangan gonad mencapai TKG-III dengan diameter rata-rata oosit G-1, G-2, dan G-3 berkisar antara 1.13 ± 0.11, 0.92 ± 0.18, dan 1.11 ± 0.10 mm dengan IGS dari G-1, G-2, dan G-3 dari 8.20%, 0.98 %, dan 4.8%, masing-masing. Tidak ada perbedaan yang signifikan antara diameter oosit G-1, G-2, dan G-3 (P£0,05). Keturunan kedua (G-2) merupakan generasi terbaik untuk dijadikan kandidat budidaya. Asian redtail catfish (Hemibagrus nemurus) has been successfully domesticated by the Research Institute for Freshwater Aquaculture and Fisheries Extension, Bogor. The results of the domestication of Cirata population have better fecundity and growth, this population has been developed for up to three generations (G1, G2 and G3) however, there is limited information regarding the comparison between the ovarian development of different generations of the fish]. This study?s aim was to measure the growth and observe the ovarian development of the first (G-1), second (G-2), and third generations (G-3). The parameters measured and observed included the measurements of length, weight, specific growth rate, morphology and gonad weight, oocyte diameter, and somatic gonad index (IGS). The sampling was carried out every two months by collecting ten fish samples of each generation. Gonad tissues were histologically prepared and stained asetocarmine solution as the coloring agent. The observations used a microscope with a magnification of 4 x 10. The results of the study showed that at the age of 222 days, the male and female gonads were physically still in the form of fine threads. However, secondary characteristics in male fish such as papillae have begun to appear though not very clear. At the age of 283 days, oocytes? sizes ranged between 0.025-0.05 mm. At the age of 345 days, the eggs were starting to be visible with the oocyte sizes of G-1, G-2, and G-3 ranged between 0.59 ± 0.24, 0.39 ± 0.15, and 0.48 ± 0.15 mm, respectively. After the age of 406 days, the development of gonads reached gonad maturating level III with the average diameter of the oocytes of G-1, G-2, and G-3 ranged between 1.13 ± 0.11, 0.92 ± 0.18, and 1.11 ± 0.10 mm with the IGS of G-1, G-2, and G-3 of 8.20%, 0.98%, and 4.8%, respectively. There was no significant difference among the oocyte diameters of G-1, G-2, and G-3 (P£0.05). The 2nd generation (G2) is the best generation to be a candidate aquaculture.
KARAKTERISASI GENETIK IKAN KELABAU (Osteochilus kelabau) DARI BERBAGAI LOKASI DI KALIMANTAN BARAT MENGGUNAKAN METODE RAPD (Random Amplified Polymorphism DNA) Kusmini, Irin Iriana; Gustiano, Rudy; Mulyasari, Mulyasari
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.725 KB) | DOI: 10.14203/beritabiologi.v10i4.762

Abstract

Kelabau fish (Osteochilus sp.) is an endemic fish to Kalimantan inland waters that is potential to be developed. The aim of this research was to characterize Kelabau fish and to study the data base (genetic character) of kelabau as well as its relationship. The result showed that the highest polymorphism and heterozigosity was on Kelabau Sintang and the lowest was on Kelabau Kapuas Hulu. The closest genetic distance value were between Kelabau Pontianak-Kapuas Hulu (0.5351) and the furthest were between Kelabau Pontianak-Sintang (0.6852).
NILAI HETEROSIS DAN PERANAN INDUK PADA KARAKTER PERTUMBUHAN HASIL PERSILANGAN INTERSPESIFIK Tor soro DAN Tor douronensis [Growth Heterosis Values and The Role of Parent Tor soro and Tor douronensis in Interspesific Crossed] Radona, Deni; Subagja, Jojo; Kusmini, Irin Iriana; Gustiano, Rudhy
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2784.425 KB) | DOI: 10.14203/beritabiologi.v15i2.2394

Abstract

Fish Tor is one of potential fish commodities to be developed because of their economic value. This on experiment was designed to evaluate the effect of male and female parent on morphological characters i.e length and weight growth and the value of heterosis from crosses of Tor soro and Tor douronensis. Crosses were performed in both directions (reciprocal) to form four populations. Observations lenght, weight gain, specific growth in length, specific growth in weight and value of heterosis were carried out for two months (January-March, 2014). The results showed an increase of  length, weight, specific growth in weight, specific growth in length occurred in seed produced from parent male of T. soro and female of T. douronensis with values of 0.54 cm, 0.09 g, 0.65 % and 3.79 %, rescpectively. Crossed of ? T. douronensis x ? T. soro yield heterosis value (partial) of length (40.90%) and weight (116.66%) was higher than crossed of ? T. soro x ? T. douronensis  that only have heterosis value of 0 and 33.33 %, and heterosis value (reciprocal) on the length of 20.45 % and the weight of 45.22 %.
RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS [Genetic Diversity of Three Populations of Snakeskin Gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan Based on RAPD Analysis and Truss Morphometrics Measurements] Iskandariah, Iskandariah; Soelistyowati, Dinar Tri; Gustiano, Rudhy; Kusmini, Irin Iriana; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.892 KB) | DOI: 10.14203/beritabiologi.v14i1.1866

Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.
ANALISIS RAGAM GENOTIP RAPD DAN FENOTIP TRUSS MORFOMETRIK PADA TIGA POPULASI IKAN GABUS [Channastriata(Bloch, 1793)] [Analysis of Genotype Variation and Truss Morphometricof Three Populations of Snakehead Fish [Channa striata (Bloch, 1793)] Gustiano, Rudhy; Oktaviani, Tia; Soelistyowati, Dinar Tri; Kusmini, Irin Iriana; Wahyutomo, Wahyutomo; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.515 KB) | DOI: 10.14203/bb.v12i3.641

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In order to manage genetic resources for aquaculture development of snakehead fishChannastriata(Bloch, 1793), genetic variability of three populations from different geographical areas is needed to be understood. The purpose of this study was to identify the genotype and fenotype of snakehead fish from Jawa, Sumatera and Kalimantan using RAPD and “Truss” morphometric. RAPD method used OPA-02, OPA-04 and OPA-07 primers.While twenty one measurement of truss morphometric was done on the body of fish observed. The results showed that population from Java had higher percentage of polymorphism and heterozygosity than those of Sumatera and Kalimantan, accounted for 83.33% and 0.3655 respectively. Population from Kalimantan and Sumatera had the lowest genetic distance of 0.1170.Meanwhile,the highest genetic distance (0.1908) was observed between population from Kalimantan and Java.Interpopulation relation based on the similarity of truss morphometric population from Sumatera and Kalimantan was 50%. However, those populations had similarity of 24.96% with population from Java. Coefficient variation of morphometric data showed that variation of population from Kalimantan was higher than those of Jawa and Sumatera.
KERAGAMAN FENOTIPA IKAN NILA BEST F4,F5 DAN IKAN NILA NIRWANA 2 HASIL SELEKSI DENGAN ANALISIS TRUSS MORFOMETRIK Kusmini, Irin Iriana; Soelistyowati, Dinar Tri; Gustiano, Rudhy; Pitriani, Peni; Prakoso, Vitas Atmadi
Jurnal Sains Natural Vol 3, No 2 (2013): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.39 KB) | DOI: 10.31938/jsn.v3i2.65

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Phenotype Diversity of BEST Nila Fish of F4, F5 and The Nirwana 2 Nila Fish Using Truss Morphometric Analysis          In the framework of the management of genetic resources for long-term development and sustainability of cultivating nila tilapia fish for the evaluation of population genetic resources needs to be done. The purpose of this research was to know the fish of nila tilapia BEST phenotype diversity of F4, F5 and the nila tilapia fish of Nirwana 2 using Truss Morfometrik. The fish from populations of Tilapia BEST fish of F4, F5 and a Nirwana 2 fish each sample taken as many as 20 tails. The measurement was done by specifying points along the body of the fish assay based on morfometrik truss method. The dots were connected one with the others so retrieved 21 characters measuring results. The analys was done using cluster analysis. The observations indicated levels of similarity of truss morfometrik BEST Fish of F4, F5 and the Nirwana 2 fish tilapia very high character except on B6, B3, A3, B1, C1 and D6 that significantly different (p ? 0.05).The results gave an indication of the quantity of fish body that  BEST fish was  shorter and rounder than the fish of Nirwana 2 tilapia. Based on the inter population relationship the fish of Nirwana 2 tilapia separated  from BEST nila of F4 and F5.Keywords : BEST tilapia, Nirwana tilapia, truss morfometrik, diversity ABSTRAK         Dalam rangka pengelolaan sumber genetik jangka panjang dan pengembangan budidaya untuk kelestarian ikan nila maka evaluasi sumber daya genetik populasi perlu dilakukan.Tujuan dari penelitian ini adalah mengetahui ragam fenotipe ikan nila BEST F4, F5 dan ikan nila Nirwana 2menggunakanTruss Morfometrik. Ikan nila dari populasi nila BEST F4,F5 dan nila Nirwana2 masing-masing diambil contoh sebanyak 20 ekor. Pengukuran dilakukan dengan menentukan titik-titikacuan sepanjang tubuh ikan uji berdasarkanmetode Truss Morfometrik. Titik-titik dihubungkan satu dengan lainnyasehingga diperoleh 21 karakter hasil pengukuran. Analisis dilakukan dengan menggunakan cluster analysis. Hasil pengamatan menunjukkan tingkat kemiripan truss morfometrik Ikan nila BEST F4, F5 dan ikan nila Nirwana 2 sangat tinggi kecuali pada karakter B6, B3, B1, A3, C1 dan D6 yang berbeda nyata (p?0,05).Hasil tersebut memberikan indikasi bahwapola badan ikan nila BEST lebih pendek dan bulat dibandingkan ikan nila Nirwana2 yang lebih panjang. Berdasarkan hubungan interpopulasi ikan nila Nirwana 2 terpisah dengan kelompok nila BEST F4 dan F5.Kata kunci : nila BEST, nila Nirwana, truss morfometrik, keragaman
PRODUKSI IKAN UNGGUL DI LAHAN MINAPADI SECARA INTENSIF Cahyanti, Wahyulia; Prakoso, Vitas Atmadi; Arifin, Otong Zenal; Kusmini, Irin Iriana
Jurnal Sains Natural Vol 4, No 1 (2014): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.811 KB) | DOI: 10.31938/jsn.v4i1.72

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Superior Fish Production in Intensive Paddy Cum Fish Culture         Aquaculture development in Indonesia is expected to push forward the fish farmers entrepreneurship and competitiveness of aquaculture products in a sustainable manner through improved efficiency and cooperative advantages. Development of fisheries resources in accordance with the management and the optimal use or to provide added value of the fish itself also to provide other products. One technology that can be used is through technology in paddy cum fish culture. This study aimed to improve of land productivity through superior tilapia production in paddy cum fish culture system. In general, the best results obtained in treatment A (no treatment). Productivity of rice and fish could be enhanced by applying paddy cum fish culture  system by 4 to 5.5 million or 10-15% per hectare rice field.Keywords: Minapadi, Productivity, Nila, Fertilizer ABSTRAK          Pengembangan perikanan budidaya di Indonesia ke depan diharapkan dapat  mendorong masyarakat perikanan/pembudidaya untuk meningkatkan jiwa kewirausahaan dan daya saing produk perikanan budidaya secara berkelanjutan melalui peningkatan efisiensi dan keunggulan koperatif. Pengembangan sumber daya perikanan yang sesuai dengan pengelolaan dan pemanfaatan yang optimal dan dapat memberikan nilai tambah selain dari ikannya itu sendiri juga produk lainnya. Salah satu teknologi yang dapat digunakan yaitu melalui teknologi minapadi. Penelitian ini bertujuan untukmeningkatkan produktivitas lahan melalui produksi ikan nila unggul dalam sistem minapadi. secara umum, hasil terbaik diperoleh pada perlakuan A (tanpa perlakuan). Dari hasil penelitian, didapatkan kesimpulan bahwa Ikan nila lebih baik pertumbuhannya dibanding mas.Secara umum hasil terbaik diperoleh dari lahan kontrol atau tanpa perlakuan. Produktivitas padi dan ikan dapat ditingkatkan dengan menerapkan sistem minapadi senilai 4-5,5 juta atau 10-15% tiap hektar sawah.Kata kunci : minapadi, produktifitas, Nila, pupuk
ANALYSIS OF GROWTH OF LALAWAK Barbonymus balleroides (VALENCIENNES, 1842) IN THREE CULTURE METHODS Radona, Deni; Prakoso, Vitas Atmadi; Kusmini, Irin Iriana
Indonesian Aquaculture Journal Vol 12, No 1 (2017): (June 2017)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.831 KB) | DOI: 10.15578/iaj.12.1.2017.15-20

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In fish culture, optimal growth could be influenced by various culture methods. Aim of the study was to evaluate the productivity of Barbonymus balleroides, lalawak in floating net cages, concrete ponds, and earthen ponds. Cultivation was designed with the circulation water system. Experiment was conducted using completely randomized design with three treatments and three replications for each treatment. The experimental fish, sized of 4.20 ± 0.64 cm SL and weight of 2.14 ± 0.99 g, were obtained from induced breeding. The stocking density used was 20 individuals/m3. Fish were fed 3% of total weight two times every day using commercial pellet with 35% protein content for 90 day. The result showed that lalawak reared in earthen pond was no significant difference on length, weight, and biomass compared with that one in concrete pond (P>0.05), but significantly different (P<0.05) with floating net cages. There were no different (P>0.05) among the three different culture systems for survival rate and FCR. Lalawak reared on earthen pond system supported with optimal water quality could increase productivity value.
KINERJA PERTUMBUHAN DAN EFISIENSI PAKAN IKAN Tor tambroides YANG DIBERI PAKAN KOMERSIAL DENGAN KANDUNGAN PROTEIN BERBEDA Radona, Deni; Subagja, Jojo; Kusmini, Irin Iriana
Media Akuakultur Vol 12, No 1 (2017): (Juni, 2017)
Publisher : Pusat Penelitian dan Pengembangan Perikanan, Badan Penelitian dan Pengembangan Kelautan da

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.454 KB) | DOI: 10.15578/ma.12.1.2017.27-33

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Protein merupakan nutrien yang sangat berperan dalam pertumbuhan ikan. Penelitian ini bertujuan mengetahui kebutuhan protein optimal untuk pertumbuhan ikan Tor tambroides dan pengaruh kandungan protein pakan terhadap efisiensi pakannya. Benih ikan Tor yang digunakan berukuran panjang (1,5 ± 0,1 cm) dan bobot (0,08 ± 0,01 g). Benih ikan dipelihara dalam akuarium berukuran 40 cm x 30 cm x 30 cm dengan ketinggian air 20 cm sebanyak 50 ekor. Selama 40 hari pemeliharaan benih ikan diberi pakan komersil berupa crumble dengan kandungan protein, (A) 25%, (B) 35%, dan (C) 50% sebanyak 20% per hari dari total biomassa ikan, pemberian pakan dengan frekuensi tiga kali sehari. Rancangan penelitian menggunakan rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan. Hasil penelitian menunjukkan ikan Tor tambroides yang diberi pakan dengan kandungan protein sebesar 35% dan 50% memiliki pertumbuhan panjang, bobot, laju pertumbuhan harian (LPH), biomassa, nisbah konversi pakan (FCR), dan efisiensi pakan yang sama (P>0,05) dan berbeda nyata pada pakan dengan kandungan protein 25% (P<0,05).Protein is a nutrient is which plays a major role in the growth of fish. This study was aimed to determine the optimal protein requirement for growing of Thai mahseer and the influence of different protein levels of feed on feed efficiency. The average length and weight of fish used were 1.5 ± 0.1 cm and 0.08 ± 0.01 g. The fish were reared in the aquarium (dimension= 40 cm x 30 cm x 30 cm filled with 20 cm of water and the stocking density of each aquarium was 50 individuals. During 40-day reared, seedling fish were fed by commercial crumble with different protein levels, (A) 25%, (B) 35%, and (C) 50%, as much as 20% of total weight every day with a feeding frequency of three times per day. This experiment was conducted by using completely randomized design (CRD) with three treatments and three replications for each treatment. The results showed that Tor tambroides fed diets with protein levels of 35% and 50% was not significantly different on the growth value (length and weight), specific growth rate, biomass, feed conversion ratio (FCR), and feed efficiency (P>0.05) and was significantly different on feed protein levels 25% (P<0.05).