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The Spermicide Effect from Methanol Extract of Kalangkala Seed (Litsea angulata) to Spermatozoa Mice (Mus musculus) Akmal, Rommy; Kuswanto, Kuswanto; Fahrunnisa, Syarifah; Rahmi, Ridha Aulia; Bayanil, Nur Ema Putri; Nurliani, Anni
Journal of Agromedicine and Medical Sciences Vol 2, No 2 (2016)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Abstract

Men involvement in family planning needs to be increased by providing natural spermicide from natural source which is safer. One of them is kalangkala seed which is endemic plant in South Kalimantan. The spermicide effect from methanol extract of kalangkala seed to mice spermatozoa was studied in this research by in vitro method. Spermatozoa suspension was taken from epididymis cauda of twenty five male Balb/c mice. This research used a complete randomized design with five treatments i.e control which without any addition; spermatozoa suspension + NaCMC 0.5%; spermatozoa suspension + methanol extract of kalangkala seed 0.1%; spermatozoa suspension + methanol extract of kalangkala seed 0.3%; and spermatozoa suspension + methanol extract of kalangkala seed 0.5% with five repetitions for each treatment. The result of this study showed that methanol extract of kalangkala seed caused decrease of motility and movement velocity mice spermatozoa until achieve zero value in 0.5% concentration. Key words: kalangkala seed, mice, spermatozoa, natural spermicide
THE POTENTIAL OF KALANGKALA (Litsea angulata) SEED EXTRACT AS A NATURAL SPERMICIDE MATERIAL Akmal, Rommy; Rusmiati, Rusmiati; Santoso, Heri Budi
TROPICAL WETLAND JOURNAL Vol 3, No 2 (2017)
Publisher : The Journal is published by Graduate Programe of Lambung Mangkurat University

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Abstract

Kalangkala (Litsea angulata) is one of the endemic plants in the wetlands area inKalimantan which seeds are can reduce the quality of male mice spermatozoa such as motility and mobility. The purpose of this research is to know the potential of kalangkala seed extract on other parameters of spermatozoa quality, which are viability and morphology of male mice spermatozoa in an effort to obtain natural spermicide material. This research used five treatments by using twentyfive male mice. The design of this research was spermatozoa suspensions without being treated (Control = 0), spermatozoa suspensions +Na-CMC 0,5% (P1), spermatozoa suspensions + extract of kalangkala seed 0,1% (P2), spermatozoa suspensions + extract of kalangkala seed 0,3% (P3), and spermatozoa suspensions + extract of kalangkala seed 0,5% (P4), with five repititions for each treatment. The result of this research showed that thekalangkala seed extract reduce the percentage of viability of spermatozoa and increased the percentage of abnormal morphology of spermatozoa male mice. Therefore, kalangkala seed extract has potential as a natural spermicide.
THE POTENTIAL OF KALANGKALA (Litsea angulata) SEED EXTRACT AS A NATURAL SPERMICIDE MATERIAL Akmal, Rommy; Rusmiati, Rusmiati; Santoso, Heri Budi
TROPICAL WETLAND JOURNAL Vol 3 No 2 (2017): Tropical Wetland Journal
Publisher : Post-Graduate ULM Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/twj.v3i2.45

Abstract

Kalangkala (Litsea angulata) is one of the endemic plants in the wetlands area in Kalimantan which seeds are can reduce the quality of male mice spermatozoa such as motility and mobility. The purpose of this research is to know the potential of kalangkala seed extract on other parameters of spermatozoa quality, which are viability and morphology of male mice spermatozoa in an effort to obtain natural spermicide material. This research used five treatments by using twentyfive male mice. The design of this research was spermatozoa suspensions without being treated (Control = 0), spermatozoa suspensions +Na-CMC 0,5% (P1), spermatozoa suspensions + extract of kalangkala seed 0,1% (P2), spermatozoa suspensions + extract of kalangkala seed 0,3% (P3), and spermatozoa suspensions + extract of kalangkala seed 0,5% (P4), with five repititions for each treatment. The result of this research showed that the kalangkala seed extract reduce the percentage of viability of spermatozoa and increased the percentage of abnormal morphology of spermatozoa male mice. Therefore, kalangkala seed extract has potential as a natural spermicide.