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PENEKANAN PERKEMBANGAN PENYAKIT BUSUK BATANG VANILI (FUSARIUM OXYSPORUM F.SP. VANILLAE) MELALUI SELEKSI ASAM FUSARAT SECARA IN VITRO Nurcahyani, Endang; Sumardi, Issirep; Hadisutrisno, Bambang; Suharyanto, E.
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 12, No 1 (2012): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.046 KB) | DOI: 10.23960/j.hptt.11212-22

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The most biological constrain on Vanilla planifolia plantation recently was caused by epidemical disease that laterdecrease vanilla production. The most important disease on vanilla is foot rot disease caused by Fusarium oxysporum f. sp.vanillae. So far, the disease has not been successfully controlled although some experiments had been conducted. Onealternative method has been introduced by using a new cultivar which was resistance to Fusarium). A mutant vanilla to thefungus has been initiated by in vitro selection on medium containing fusaric acid. The aims of this research were: (1) to investigate effective concentration of fusaric acid used for in vitro selection, (2) to characterize mutants which have been set up and also to test those mutants for their resistance to the fungus. The results showed that: (1) fusaric acid at the concentration of 110 ppm effectively suppressed the disease intensity up to 25% compared to the concentration of 90 ppm and 100 ppm. In other words, 110 ppm of fusaric acid has increased the category criterion from moderate to resistant, (2) there was an increase of the total phenol content and thickness of lignin in vanilla stem, and (3) the protein profile of vanilla plantlet was different from the control. There was an initiation of a new band of about 18 kD in a mutant predicted as a protein which is responsible for vanilla resistance to Fusarium.
EFEKTIVITAS EKSTRAK DAGING BUAH NANAS (ANANAS COMOSUS L.) DALAM PENURUNAN INDEKS BROWNING DARI UMBI KENTANG (SOLANUM TUBEROSUM L.) Wardanis, Putri; Zulkifli, Zulkifli; Lande, Martha L.; Nurcahyani, Endang
Jurnal Penelitian Pertanian Terapan Vol 19, No 2 (2019)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jppt.v19i2.1568

Abstract

The purpose of this study was to determine whether the pineapple fruit extract can inhibit theprocess of browning on the potato tubers. This study used Completely Randomized Design(RAL) with 5 levels of pineapple fruit concentration of 0% v/v, 25% v/v. 50% v/v, 75% v/v,and 100% v/v and consist of 5 replications. Qualitative Parameters in this study was the colosurface of potato tubers while quantitative parameters were browning index, total soluble carbohydrate content, and dehydrogenase enzyme activity homogeneity of variance, analysisof variance, and Tukey test were conducted at 5% significant level. Correlations betweendependent and independent variables were determined by linear regression. The resultshowed that the color surface of potato tuber treated with the concentration of pineappleextract 100% v/v was less brown than control and other concentrations. Index browning ofpotato tuber treated with concentration 75% v/v and 100% v/v was significantly decreased. Concentration of pineapple extract was negative linearly correlated to browning index ofpotato tubers. The concentration of pineapple extract 100% v/v increased significantly totalsoluble carbohydrate of potato tubers. The concentration of pineapple extract was positivelinearly correlated to total soluble carbohydrate. The activity of dehydrogenase enzyme ofpotato tuber treated with concentration 100% v/v was significantly increased. Theconcentration of pineapple extract was quadratic correlated to the activity of dehydrogenaseenzyme.
Aktivitas Enzim Peroksidase Daun Planlet Pisang Ketan (Musa paradisiaca L.) Hasil Pengimbasan Ketahanan terhadap Asam Salisilat secara In Vitro Muslimah, Imamah; Nurcahyani, Endang; Zulkifli, Zulkifli
Jurnal Penelitian Pertanian Terapan Vol 17, No 2 (2017)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.827 KB) | DOI: 10.25181/jppt.v17i2.288

Abstract

Banana plant production are decreased because it getting an attack from Fusarium oxysporum. “ketan” Banana plantlet that resistant to the Fusarium oxysporum were selected by in vitro in the solid Murashige and Skoog (MS) medium with added salicylic acid at 40 ppm, 50 ppm, 60 ppm, and 70 ppm concentration, compared with controls (0 ppm). The aim of this research is to determine the activity of peroxidase enzyme on leaves of banana ketan plantlet. The research was carried out in December 2015 to February 2016 in the Laboratory of Botani (in vitro room), Departement of Biology, Faculty of MIPA, Lampung University. This study used a completely randomized design with 5 replications. Data were analyzed with the variance (Anova) and if them different will be continued by LSD test performed at 5% significance level. Extraction of peroxidase method using Saravanan et al. (2004) with the mixture of pyrogallol and 𝐻2𝑂2. The absorbance was measured with a spectrophotometer (Shimudzu UV 800) at wavelength (λ) of 420 nm and readable from zero. The result showed that the activity of peroxidase enzyme on the leaves banana ketan plantlet has increased optimally in the salicylic acid concentration of 50 ppm compared with controls. Increased of Peroxidase enzyme activity showed that banana ketan plantlet was resistant to the salicylic acid and expected to be resistant to Fusarium oxysporum. Keywords: Salicylic acid, Peroxidase enzyme, Fusarium oxysporum, in vitro, Banana ketan
Uji Ketahanan Planlet Anggrek Bulan (Phalaenopsis amabilis (L.) Bl.) Hasil Seleksi dengan Asam Salisilat Terhadap Fusarium oxysporum Secara In Vitro Noviantia, Ria Aulia; Nurcahyani, Endang; Lande, Martha Lulus
Jurnal Penelitian Pertanian Terapan Vol 17, No 2 (2017)
Publisher : Politeknik Negeri Lampung.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.099 KB) | DOI: 10.25181/jppt.v17i2.292

Abstract

Phalaenopsis amabilis cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum. Plantlet P.amabilis which resistant to F. Oxysporum was selected in the solid Vacin and Went (VW) medium was added with salicylic acid at concentrations of 65 ppm, 75 ppm, and 85 ppm, compared with controls (0 ppm). The goals of the research were to study and determine of: 1) The SA concentration of planlet P. amabilis selection tolerant; 2) The proper concentration of AS during in vitro selection for suppressing the Fo. The research was carried out in December 2015 to February 2016 in the Botany Laboratory (in vitro research room), Departement of Biology, Faculty of MIPA, Lampung University. The result showed that: 1) The SA tolerant concentration for plantlet selection with P. amabilis was between 65 ppm - 85 ppm. 2) The 85 ppm of SA was effective for suppressing the Fo compared to 65 ppm and 75 ppm. Keywords: Phalaenopsis amabilis, salicylic acid, Fusarium oxysporum, In vitro, Resistance.
Uji Potensi Bioherbisida Ekstrak Daun Mahoni (Swietenia mahagoni (L.) Jacq) Terhadap Pertumbuhan Gulma Maman Ungu (Cleome rutidosperma D.C.) Kurniawan, Agung; Yulianty, Yulianty; Nurcahyani, Endang
Biosfer: Jurnal Tadris Biologi Vol 10, No 1 (2019): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v10i1.4232

Abstract

Penelitian ini bertujuan untuk menguji ekstrak daun mahoni (Swietenia mahagoni (L.) Jacq.) terhadap pertumbuhan gulma maman ungu (Cleome rutidosperma D.C.). Penelitian menggunakan metode RAL (Rancangan Acak Lengkap) dengan konsentrasi 0%, 10%, 20%, 30%, 40%, 50%, dan 60% dengan ulangan sebanyak 4 kali. Variabel dalam penelitian ini adalah tinggi tanaman, berat basah, jumlah helai daun, kandungan klorofil a, kandungan klorofil b, dan kandungan klorofil total. Uji homogenitas dilakukan menggunakan uji Bartlett, selanjutnya dianalisis menggunakan metode statistik ANOVA (Analysis of Variance) pada taraf nyata 5% (p < 0,05) jika terdapat beda nyata dilanjutkan dengan uji BNT pada taraf nyata 5%. Hasil penelitian menunjukkan ekstrak daun mahoni dengan konsentrasi 10% efektif untuk menghambat pertumbuhan tinggi dan jumlah helai daun, sedangkan konsentrasi 20% efektif untuk menghambat berat basah gulma maman ungu. Semakin tinggi konsentrasi yang digunakan maka semakin efektif ekstrak daun mahoni untuk menghambat pertumbuhan gulma maman ungu. Ekstrak daun mahoni belum mempengaruhi kadar klorofil gulma maman ungu.
Efek Ekstrak Metanol Makroalga Cokelat (Sargassum sp.), Merah (Gracillaria sp.) dan Taurin Terhadap Gambaran Histopatologi Hepar Mencit Jantan (Mus musculus) yang Diinduksi Benzo(?)Piren Hervidea, Radella; Widiastuti, Endang Linirin; Nurcahyani, Endang; Sutyarso, Sutyarso; Susanto, G. Nugroho
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3670

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ABSTRACTCancer is a disease marked by the existence of abnormal growth of cell continuously and uncontroll in an organism body. Benzo(?)pyrene is one of the substances that can cause a cancer. The methanolic extract of Sargassum sp. and Gracillaria sp. has anticancer and antioxidant activity. Other compounds with antioxidant activity is taurine. The aim of this research is to examine the effect from methanolic extract of Sargassum sp. and Gracillaria sp .and also taurine on hepar histopatology of male mice (Mus musculus) induced by benzo(a)pyrene. This research used Completely Randomized Design. 30 male mice were divided into 6 groups of treatments, those were: K1 = (K-), K2= induced by benzo(?)pyrene for 10 days, K3= after induced by benzo(?)pyrene, were given the Sargassum sp. orally with a dose 8mg/mice during 15 days, K4=after induced by benzo(a)pyrene, were given the combination of Sargassum sp. (8mg/mice) and taurine(15,6mg/mice) orally during 15 days, K5= after induced by benzo (?)pyrene, were given the Gracillaria sp.orally with a dose 8mg/mice during 15 days, and K6=after induced by benzo(a)pyrene, were given the combination of Gracillaria sp. (8mg/mice) and taurine (15,6mg/mice) orally during 15 days. Data were analyzed using ANOVA (? = 5%). The result shows that the combination of Sargassum sp. and Gracillaria sp. and taurine can increase the number of erythrocyte , decrease the number of leucocyte and protect the damage of hepar histopatology of male mice (Mus musculus) induced by benzo (?)pyrene. Combination of extract Gracillaria sp. and taurine is the most effective to protect the damage hepar histopatology of male mice(Mus musculus) induced by benzo(? )pyrene.Keywords: male mice,Sargassum sp., Gracillaria sp., taurine, benzo(a)pyrene, hepar
The influence of magnetic field on the growth of tomato (Lycopersicum esculentum) infected with Fusarium oxysporum Agustrina, Rochmah; Nurcahyani, Endang; Pramono, Eko; Listiana, Ika; Nastiti, Eko
INSIST Vol 1, No 1 (2016)
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ins.v1i1.16

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Abstract— This Previous research suggests that magnetic field (MF) can enhance seedling vigor, growth, and the production of tomato plants. This study examines the influence of MF on the growth of tomato infected by Fusarium oxysporum, fungal pathogen that causes Fusarium wilt in tomatoes. This factorial study arranged in a stripe split plot design. Factors tested consists of long exposure to the MF, M (control; 7'48 "; II'42" and 15'36 "), how to infect fusarium, F (through soaking the seeds and by infecting fusarium through the stem at the age 28 days after sowing (das), and soaking the seeds, S (soaking and without soaking for 15 minutes before MF treatment). Each unit of experiment was repeated 3 times. Parameters measured were percentage of germination, growth rate based on plant height, and fresh weight. Observations were made on the plant until 42 das. Data were analyzed of variance followed by the least significant difference (LSD) test at α = 1 and 5%. The results showed that the interaction between MF and fusarium (MxF), fusarium and soaking (FxS), and MF, fusarium, and soaking (MxFxS) did not produce a different response on all parameters measured. Differences in response to the treatments derived from the treatment of MF (M), Fusarium (F), and the treatment interaction of the MF x soaking (MxS). The MF (M) and the interaction of MF and soaking (MxS) significantly effect on all parameters measured at each measurement. The treatment of Fusarium (F) significantly effect on germination percentage, and fresh weight at 7 das.Keywords— magnetic field, fusarium, germination percentage, growth rate, fresh weight.
EFEKTIVITAS PEMBERIAN PUPUK ORGANIK CAIR DARI TIGA JENIS REBUNG BAMBU TERHADAP PERTUMBUHAN TANAMAN TOMAT (SOLANUM LYCOPERSICUM MILL.) Setiawan, Alfian Bagus; Yulianty, Yulianty; Nurcahyani, Endang; Lande, Martha Lulus
Biosfer: Jurnal Tadris Biologi Vol 10, No 2 (2019): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v10i2.4591

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui efek dari pemberian pupuk organik cair rebung bambu terhadap pertumbuhan tanaman tomat (Solanum lycopersicum Mill.) dan mengetahui konsentrasi yang paling baik untuk pertumbuhannya. Penelitian ini telah dilaksanakan pada bulan Januari 2018 sampai bulan Februari 2018. Penelitian dilakukan di laboratorium lapang terpadu, Fakultas Pertanian Universitas Lampung dan laboratorium botani Fakultas Matematika dan Ilmu Pengetahuan Alam. Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 16 perlakuan. Rebung Bambu Betung (Dendrocalamus asper (Schult) Backer e.x Heyne), rebung Bambu Tali (Gigantochloa apus (Schulft.f.) Kurz.), dan rebung Bambu Kuning (Bambusa Vulgaris) dengan konsentrasi pupuk organik cair masing-masing jenis bambu terdiri dari 6 konsentrasi yaitu : kontrol (0%), 5%, 10%, 15%, 20%, dan 25%, dilakukan ulangan sebanyak 2 kali. Analisis data menggunakan langkah-langkah yaitu uji homogenitas menggunakan uji levene dengan taraf 5% dan uji ragam (ANOVA). Hasil penelitian menunjukkan perlakuan pupuk organik cair rebung bambu betung dengan konsentrasi 10% (A1B2) perlakuan yang terbaik terhadap tinggi tanaman, panjang akar, berat kering, kandungan klorofil a dan kandungan klorofil b, namun tidak berpengaruh terhadap kandungan klorofil total tanaman tomat.
PENGARUH LARUTAN ATONIK TERHADAP KANDUNGAN KARBOHIDRAT TERLARUT TOTAL PLANLET JERUK SIAM PONTIANAK (Citrus nobilis Lour. var. microcarpa Hassk.) SECARA IN VITRO Rosyalina, Nadya; Nurcahyani, Endang; Qudus, Hardoko Insan; Zulkifli, Zulkifli
Analit: Analytical and Environmental Chemistry Vol 3, No 1 (2018): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Jeruk siam pontianak (Citrus nobilis Lour. var. microcarpa Hassk.) salah satu komoditas buah-buahan penting di Indonesia. Jeruk yang mempunyai nilai ekonomis yang cukup tinggi baik dalam bentuk segar maupun olahan serta sebagai sumber vitamin dan mineral. Penelitian ini bertujuan untuk mengetahui konsentrasi larutan atonik yang optimum; konsentrasi Polyethylene Glycol (PEG) yang toleran terhadap cekaman kekeringan untuk seleksi planlet jeruk siam secara in vitro; mengetahui interaksi antara larutan atonik dengan PEG 6000 untuk mengetahui dan menganalisis karakter ekspresi spesifik pada planlet jeruk siam yang toleran terhadap cekaman kekeringan meliputi kandungan karbohdrat terlarut total. Penelitian ini dilakukan dalam percobaan faktorial 3 x 3. Faktor A adalah larutan atonik dengan 3 taraf konsentrasi : 0 mL/L, 1 mL/L, dan 2 mL/L. Faktor B adalah PEG 6000 dengan 3 taraf konsentrasi: 0% b/v, 3 % b/v, dan 5 % b/v. Uji Levene, analisis ragam pada taraf nyata 5%. Analisis ragam dilakukan pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa larutan atonik dan tingginya tingkat kekeringan berpengaruh nyata menurunkan kandungan karbohidrat terlarut total. Dari hasil penelitian dapat disimpulkan bahwa larutan atonik tidak berpengaruh nyata dalam peningkatan kandungan karbohidrat terlarut total pada planlet jeruk siam Pontianak.?Doi:?http://dx.doi.org/10.23960/aec.v3.i1.2018.p61-68
ANALISIS KANDUNGAN PROLIN PLANLET JERUK KEPROK BATU 55 (Citrus reticulata Blanco var. crenatifolia) SETELAH DIINDUKSI LARUTAN ATONIK DALAM KONDISI CEKAMAN KEKERINGAN SECARA IN VITRO Ashari, Anis; Nurcahyani, Endang; Qudus, Hardoko Insan; Zulkifli, Zulkifli
Analit: Analytical and Environmental Chemistry Vol 3, No 1 (2018): Analit: Analytical and Environmental Chemistry
Publisher : Universitas Lampung Jl. Prof. Dr. Sumatri Brojonegoro No.1 Bandar Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Jeruk keprok batu 55 merupakan salah satu tanaman yang dibudidayakan di Indonesia umumnya didaerah Jawa Timur yang berada pada ketinggian 700 - 1200 m dpl. Salah satu masalah utama budidaya jeruk di Indonesia adalah terdapat beberapa daerah di Indonesia yang kondisi tanahnya kering. Penelitian ini bertujuan untuk mengetahui larutan atonik yang optimum dan konsentrasi PEG yang toleran untuk pertumbuhan planlet jeruk keprok batu 55 (Citrus reticulata Blanco var. crenatifolia) terhadap cekaman kekeringan menggunakan (PEG) 6000, serta mengetahui karakter spesifik pada planlet jeruk keprok batu 55 meliputi kandungan prolin. Penelitian ini telah dilaksanakan pada bulan November-Desember 2017 di Laboratorium Botani Ruang Kultur Jaringan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Lampung menggunakan Rancangan Acak Lengkap Faktorial, yang terdiri dari 2 faktor yaitu faktor A larutan atonik (0 mL/L, 2 mL/L, 3 mL/L), faktor B konsentrasi PEG ( 0%, 2%, 4% ), sehingga didapatkan 9 kombinasi perlakuan yang masing-masing perlakuan di ulang sebanyak 3 kali. Homogenitas ragam menggunakan uji Levene dilanjutkan dengan analisis ragam taraf nyata 5% dan uji lanjut dengan BNT taraf nyata 5%. Hasil Penelitian menunjukkan bahwa konsentrasi larutan atonik yang optimum adalah 3 mL/L, sedangkan konsentrasi PEG 6000 yang toleran terhadap seleksi planlet jeruk keprok batu 55 adalah 4%. Karakter ekspresi planlet jeruk keprok batu 55, semakin tinggi konsentrasi PEG 6000 maka kandungan prolin semakin meningkat.?Doi:?http://dx.doi.org/10.23960/aec.v3.i1.2018.p69-78