Juni Safitri Muljowati, Juni Safitri
Faculty of Biology, University of Jenderal Soedirman, Purwokerto, Indonesia

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Penggunaan Ekstrak Rimpang Lengkuas untuk Mengendalikan Busuk Leher Akar pada Tanaman Terong (Solanum Melongena L.) Muljowati, Juni Safitri; Sucianto, Eddy Tri
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 29, No 2 (2012)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2012.29.2.243

Abstract

A research to find antifungal activity of galanga rhizome  (Alpinia galanga L.) on Sclerotium rolfsii  as the causal agent of white mold and stem rot disease on eggplant has been done.  The galanga extract was obtained by extracting the rhizome using ethanol solvent.  The experiment design was Completely Randomized Design (CRD) in a factorial pattern, involving two factors, i.e. the rhizome extract concentration (E) with six levels of 0%, 5%, 10%, 15%, 20%, and 25%; and application time (W) with two levels of  three days interval and seven days interval.  The result showed that the best extract concentration was 20% that was applied in three days interval.
Pemanfaatan Ekstrak Daun Nimba (Azadirachta indica A. Juss) untuk Pengendalian Penyakit Layu Fusarium pada Tanaman Tomat (Lycopersicum esculentum Mill.) Muljowati, Juni Safitri; Mumpuni, Aris
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 24, No 2 (2007)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2007.24.2.275

Abstract

The aim of this study was to find out the effect of neem leaf extract (Azadirachta indica A. Juss.) on the growth medium of tomato plant toward fusarial wilt and to determine effective concentration and time of application of the extract to control the wilt. Experiments arranged in a Completely Randomized Design (CRD) was employed for in vitro antimicrobial assay and in a Randomized Complete Block Design (RCBD) for in planta antimicrobial assay, both of which were to find out the inhibitory effect of the extract on colony growth of Fusarium oxysporum (Schlecht). f.sp. lycopersici (Sacc.) Snyd. et Hans. (Fol).  The results indicated that neem leaf extract concentration of 5% applied at 7 DAP (Day After Planting) was the most effective treatment in inhibiting disease intensity of fusarial wilt on tomato plant showing inhibitory effect of up to 89.32%.
Pengaruh Kombinasi Jenis Bahan Pembawa dan Lama Masa Simpan yang Berbeda terhadap Produksi Pelet Biofungisida Trichoderma harzianum Muljowati, Juni Safitri; Purnomowati, Purnomowati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 27, No 1 (2010)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2010.27.1.188

Abstract

This research aimed to know effect of carrier material combination and different storage duration on viability of T. harzianum, also to know the material type and storage duration which yielding highest conidia.  Method used in this research was experimental by using Completely Randomized Design (CRD) with factorial pattern. The first factor was type of carrying material that were white sticky rice meal, mix of 75% white sticky rice meal and 25% mungbean meal, mix of 75% white sticky rice meal and 25% soybean meal, and mix of 75% white sticky rice meal and 25% skim milk that each of such material was inoculated by 108 conidia/ml of T. harzianum with storage duration levels that were 0 weeks, 3 weeks, 6 weeks, and 9 weeks.  Obtained data was analyzed by analysis of variance (F test) with significantly level 95% and 99% and followed by Honestly Significant Difference (HSD).  Result showed that there was interaction between types of carrying material with pellet storage period that showed significant effect on viability of T. harzianum.  Carrying material type of white sticky rice meal with pellet storage period of 9 weeks resulting highest percentage acerrage of conidia seedling (viability) of T. harzianum that was 78,19%.
Longevitas dan Efikasi Pelet Trichoderma harzianum terhadap Sclerotium rolfsii Penyebab Penyakit Layu pada Tanaman Tomat (Solanum lycopersicum L.) Muljowati, Juni Safitri; Dwiputranto, Uki; Budisantoso, Iman
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.221

Abstract

Trichoderma harzianum is one of the antagonist fungi that can be used as a biofungicide to control soil-borne pathogens such as Sclerotium rolfsii, the cause of wilt isease of tomato. To ease the application of T.harzianum fungi in the field, it should be prepared a formulation in form of pellet. The longevity of T.harzianum pellet is determined by the viability of T.harzianum fungi contained in it, whereas the efication/ effectivity of T.harzianum pellet is determined by the longevity of the pellet.  The objectives of this study are to investigate the interaction between storage period and application dosages in controlling wilt disease of tomato;  and the dosage and storage period of T.harzianum on the effectiveness in controlling wilt disease of tomato. The experiment was carried out experimentally by using a Completely Randomized Design (CRD) in a factorial pattern. The first factors were storage period (W) consisted of 5 levels, i.e. 0, 3, 6, 9, and 12 weeks; the second factors were theapplication dosages (D) consisted of 6 levels, i.e. 0, 25, 50, 75, 100, and 125 grams. They were replicated 3 times each. The viability observation of T.harzianum was completed at the end of storage period of the pellets, the disease occurence and severity was since the 4th day after inoculation with 4 day interval until the tomato plant produced flowers. Research result showed that the interaction between storage period and different application dosages of T.harzianum pellets has influenced the increase of wilt disease control of tomato. Furthermore, storage period of 6 weeks and T.harzianum application of 50 g were the most effective in controlling wilt disease of tomato.
Effectiveness of Pleurotus ostreatus Extract Through Cytotoxic Test and Apoptosis Mechanism of Cervical Cancer Cells Ekowati, Nuraeni; Mumpuni, Aris; Muljowati, Juni Safitri
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7546

Abstract

Pleurotus ostreatus is a common mushroom cultivated in Indonesia, and potential properties of bioactive compounds for medicinal mushroom. This study was aimed at obtaining P.ostreatus extract bioactive compounds potential in inhibiting the proliferation of cervical cancer cells (HeLa) and evaluating the HeLa cell proliferation kinetics and HeLa cell death mechanisms. The research was beneficial in making this product can be easily applied in a more controlled industrial scale. Anticancer activity test through a cytotoxic test using the MTT [3- (4,5-dimetiltiazol-2-yl) -2.5-diphenyl tertrazolium bromide], the kinetics proliferation of HeLa cells and HeLa cell death mechanism was performed. Linear regression analysis was used to analyze the data. Ethyl acetate extract of P. ostreatus isolated from Madiun showed the best results with IC 50 = 107.59 g / ml. HeLa cell proliferation kinetics analysis showed that the application of bioactive compounds 100 g / ml resulted in an increase of in death of HeLa cells along with length of incubation time. An important finding was that HeLa cells death by apoptosis was greater than by necrosis. In conclusion, the extracts of P. ostreatus has the potential to inhibit the growth of HeLa cells.
The Structural Resistance’s Anatomy of Sweet Potato Leaves to Fungal Pathogen Sphaceloma batatas Samiyarsih, Siti; Juwarno, Juwarno; Muljowati, Juni Safitri
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12116

Abstract

Anatomical characters can be used as instructions to the structural resistance of plants to pathogen attack. Various pathogens attack sweet potato plants, such as the Sphaceloma batatas fungus that causes scurvy disease (scab). The aims of this research t test the structural resistance of sweet potato plants based on leaf anatomical character and intensity of disease attack due to the inoculated of S. batatas. The research was conducted from June to November 2016, using the Completely Randomized Design Experimental (RAL) method with a factorial pattern. The first factor was ten sweet potato cultivars and the second factor was the inoculum of S. batatas fungus each treatment with five replications. The character of leaf anatomy observed was thick of cuticle, thick of mesophyll, size and number of stomata and number of trichomes per 1 mm2 leaf area. Based on the research result, it was concluded that the inoculation of the fungus of S. batatas caused the decrease of stomata length and width on ten sweet potato cultivars. The highest intensity of disease attack was 14.33% and correlated with stomata length (r = 0.49). The anatomical structural resistance to scurvy can be used as a basis for determining crosses for obtaining superior sweet potato cultivars. The benefit of the research is to advise the community to cultivate sweet potatoes that have structural resistance to scurvy, such as cuticle and thick mesophyll, high trichomata density.
HUBUNGAN INTENSITAS PENYAKIT KARAT DENGAN PRODUKTIVITAS TANAMAN KEDELAI (Glycine max (L.) Merr.) PADA BEBERAPA VARIETAS BERBEDA Maman, Maman; Muljowati, Juni Safitri; Rochmatino, Rochmatino
Scripta Biologica Vol 1, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.2.549

Abstract

The severity of plant disease is closely related to the development of the disease itself, including rusts caused by pathogenic fungi. This research aimed to determine the difference of rusts disease severity and to determine the correlation between rusts disease severity with the productivity of different varieties of soybean. The material used was inoculum of rusts pathogenic fungi, Phakopsora pachyrhizi Syd., and four varieties of soybean, i.e., Slamet, Lokon, Bromo, and Ringgit. This research was a split-plot design experimental with subplot was four different varieties of soybean, and the main plot was the control without the treatment of inoculation (In0). The spores suspension density of inoculum of P. pachyrhizi Syd applied was 104 spores/ml (In1). The main parameters observed in this study were the height of plants, the number of pods per plant, the number of seeds per plant, the wet and dry weight of the plant, and the total weight of 100 grains seed. The supporting parameters were the disease severity, the soil acidity, the humidity, and the air temperature. The results showed the plant with lowest rusts disease severity was Slamet variety (18,38%), and the highest was Ringgit variety (35,92%). The correlation test showed the increment of rusts disease severity reduced the number of pods and dry weight of the plant in Lokon variety.
EFEKTIVITAS PELET BIOFUNGISIDA Trichoderma harzianum MENGENDALIKAN Fusarium sp. PENYEBAB PENYAKIT REBAH SEMAI PADA BIBIT TANAMAN CAISIM (Brassica rapa var. parachinensis L) Fadhilah, Rubiati; Muljowati, Juni Safitri; Purwati, Endang Sri
Scripta Biologica Vol 1, No 3 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2014.1.3.555

Abstract

This research aimed to determine the effect of the interaction of the dosage with the application model of Trichoderma harzianum bio-fungicide pellet to the effectiveness of damping-off disease control caused by Fusarium sp. on the green mustard, and also to determine the most effective dosage and application model to control that disease.  This research was experimental with the factorial completely randomized design. The factors were: the dosage of T. harzianum bio-fungicide pellet, i.e., 0 g (D0), 12,5 g (D1), 25 g (D2), 37,5 g (D3), 50 g (D4), 62,5 g (D5), and 75 g (D6); and the application model of pellet, i.e., T1, T2, T3 dan T4. This research analyzed the data using the test of variety difference with 5% and 1% degree of errors, followed by least significance difference test. The results showed the interaction between dosage and application model did not affect the effectiveness of the damping-off disease control on the green mustard seedling. The dosage of T. harzianum pellets and the application model of T. harzianum pellets independently gave a significant effect on the effectiveness of biological control agent of T. harzianum toward Fusarium sp. on the green mustard seedling. The dosage of 37,5 g per 50 green mustard individuals was the most efficient amount to control the damping-off disease. The 7-day continuous application and incubation of pellet to the seeding medium before pathogen inoculation and planting were better than any other application models.
KORELASI KARAKTER ANATOMI DAUN UBI JALAR (Ipomoea batatas L.) KULTIVAR TAHAN DAN TIDAK TAHAN TERHADAP INTENSITAS PENYAKIT KUDIS DAUN Pradana, Ade Winda; Samiyarsih, Siti; Muljowati, Juni Safitri
Scripta Biologica Vol 4, No 1 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.sb.2017.4.1.381

Abstract

Sweet potato (Ipomoea batatas L.) is one of alternative food sources beside than rice. Sweet potatoes are contains minerals, nutrients, sources of energy, protein, vitamins A and C. Sweet potatoes have lower productivity than rice and cassava. The low productivity of sweet potatoes due to several factors, which one of them is a leaf scab disease caused by pathogens Sphaceloma batatas Saw. The purpose of this study is to determine the character of the anatomy of sweet potato leaf cultivars that resistant and unresistant to the intensity of leaf scab disease, as well as the correlations between the anatomy character of sweet potato leaves with leaf scab disease intensity. The method used in this study is an experimental method with factorial completely randomized design. The first factor is the character of the anatomy of four cultivars sweet potato leaves, Cangkuang and Sukuh cultivars (cultivars resistant), cultivars Cilembu and Beta (unresistant cultivars). The second factor is the inoculation treatment S. batatas Saw. The parameters were observed thickness of cuticle, epidermis, mesophyll, the size (length and width) stomata, density of stomata and trikomata as well as the intensity of the disease were analyzed using analysis of variance (ANOVA). To determine the correlation between the anatomy character leaves with disease intensity using regression correlation analysis. The results showed that Cangkuang cultivar has cuticle, epidermis and mesophyll thickest. Beta cultivars have stomata size of the longest and widest. Cilembu cultivar has the highest density of stomata. Sukuh cultivars has the highest density. The anatomy characters include of a thick cuticle, epidermis, stomata size (length and width), as well as the density of stomata and trikomata correlated with intensity of leaf scab disease.