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Analisis Fasilitas Parkir Obyek Wisata Goa Gong, Pacitan Sulistiani, Sulistiani; Munawar, Ahmad
Jurnal Rekayasa Sipil Vol 14, No 1 (2018)
Publisher : Civil Engineering Departement, Andalas University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrs.14.1.1-12.2018

Abstract

Kabupaten Pacitan dikenal dengan “Kota 1001 Goa” dan salah satu ikonnya adalah Goa Gong. Ketersediaan fasilitas parkir yang memadai merupakan salah satu faktor yang menentukan tingkat kunjungan wisatawan. Bertambahnya jumlah wisatawan berpengaruh terhadap volume parkir. Penelitian ini bertujuan untuk mengevaluasi fasilitas parkir di Objek Wisata Goa Gong dan memberikan alternatif penanganan masalah fasilitas parkir dan aksesibilitas untuk pengembangan Objek Wisata Goa Gong. Data primer diperoleh dengan melakukan survei lapangan yang dilaksananakan pada hari Sabtu dan Minggu, berupa data nomor plat kendaraan, waktu kendaraan masuk dan waktu kendaraan keluar area parkir. Data sekunder diperoleh dari instansi terkait berupa data volume parkir tahun 2011-2016. Prediksi volume parkir tahun 2022 dilakukan dengan metode regresi berdasarkan data volume parkir tahun 2011-2016.Berdasarkan hasil analisis data, indeks parkir rata-rata di area parkir Objek Wisata Goa Gong tidak terlalu tinggi, tetapi pada jam-jam tertentu indeks parkir mencapai lebih dari 70 %. Jika indeks parkir lebih dari 70 %, pengguna parkir akan kesulitan dalam memperoleh ruang parkir. Hasil prediksi volume parkir pada tahun 2022 mencapai 20868 kendaraan untuk sepeda motor, 19776 kendaraan untuk mobil, dan 6120 untuk bus. Prioritas penanganan masalah adalah penambahan kapasitas parkir termasuk penyediaan fasilitas pendukung lainnya, misalnya pemisahan pintu masuk dan keluar area parkir, pemasangan mesin/pintu parkir otomatis pada pintu masuk dan keluar area parkir, pembuatan zebra cross serta penyediaan angkutan untuk wisata. Penerapan manajemen parkir dan penegakan peraturan yang tegas juga diperlukan dalam upaya pengembangan Objek Wisata Goa Gong.
PELAKSANAAN PERJANJIAN KERJA ANTARA KARYAWAN DENGAN PT. BAHARI SANDI PRATAMA PEKANBARU DIKAITKAN KINERJA KARYAWAN Sulistiani, Sulistiani; Jayakusuma, Zulfikar; Dasrol, Dasrol
Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum Vol 6, No 2 (2019): Juli - Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Ilmu Hukum

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Abstract

According to Law Number 13 of 2003 concerning Manpower contains the rights and obligations of workers. One of the obligations of workers contained in this Manpower Act, namely in Article 102 paragraph 2, the performance of workers is the obligation of workers after the company gives its rights to workers, PT. Bahari Sandi Pratama Pekanbaru is a sea transportation shipping company that has a target every year. The purpose of this study is to determine the implementation of work agreements between employees and PT. Bahari Sandi Pratama Pekanbaru is associated with employee performance. This type of research is classified in the type of sociological legal research, namely research on the effectiveness of existing law, the nature of this research is descriptive research that describes systematically, the facts and characteristics of the object under study appropriately. Research conducted at PT. Bahari Sandi Pratama Pekanbaru, while the population and sample are all parties involved in this study, both the chief financial officer and employees. Sources of data used are primary and secondary data, data collection techniques used by interviews, questionnaires and literature review. From the results of the research problem there are two main things that can be concluded, First, the implementation of the work agreement between the employee and PT. Bahari Sandi Pratama Pekanbaru was not carried out properly. Employees have defaulted by not carrying out what was promised. Secondly, internal factors, namely employees themselves, excessive workloads which lead to concurrent positions and multiple duties and the lack of strict application of sanctions from the company itself, listen to external factors namely a less comfortable work environment that does not increase employee performance. The author's suggestion, First, should the parties in the work agreement must fulfill what is their rights and obligations as agreed between the employee and PT. Bahari Sandi Pratama Pekanbaru. Second, to minimize the causes of employees not improving performance at PT. Bahari Sandi Pratama Pekanbaru should the parties be able to pursue all rights and obligations properly for the good of both parties Keywords: work agreements, performance, employees, employer.
ANALISIS USAHA TANI JAGUNG (ZEA MAYS L) DI DESA KUWOLU KECAMATAN BULULAWANG KABUPATEN MALANG Sulistiani, Sulistiani
ZIRAA'AH MAJALAH ILMIAH PERTANIAN Vol 40, No 3 (2015): Ziraa'ah Vol 40 No. 3 Oktober 2015
Publisher : Pusat Penerbitan Ilmiah Universitas Islam Kalimantan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/zmip.v40i3.238

Abstract

This article aim to know the efficiency of  corn production  to achieve the maximal corn product in relation using surface of farmland   in which can influences the level of production of corn in Kuwolu village of Kecamatan Bululawang of Kabupaten Malang. Result of analysis of farmer corn production in the village of  Kuwolu showed that there was a relation between production level of corn with the measure of surface of farmland of farmer.  The relation be measured with R/C rasio. According to result of analysis of R/C rasio we can understand that the wide surface of farmland of farmer can not improve the level of corn production (Rp. 2.879.114,62 ha-1 with R/C rasio 4,00), while narrow surface of farmland of farmer could improve the level of corn production (Rp. 3.054.909,12 ha-1 with R/C rasio 3,43). Because on the wide surface of farmland the program of cultivation of corn not done at all of surface of farmland.  For the farm of narrow tight agriculture, corn planted at all of its surface. Pursuant to the result of analysis, be suggested to goverment  to be can compile the agriculture policy to increase corn production for the narrow farmland and also wide farmland to improve the production of corn further
KARAKTERISASI PROTEASE BACILLUS SUBTILIS A1 INACC B398 YANG DIISOLASI DARI TERASI SAMARINDA Soeka, Yati Sudaryati; Sulistiani, Sulistiani
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.694

Abstract

Proteases is enzyme that breaks the peptide bond to produce amino acids and simpler peptides. This enzyme can be isolated from a variety of sources such as plants, animals and microbe. Alkaline proteases of microbial origin possess considerable industrial potential due to their biochemical diversity and wide applications in tannery, food, medicinal formulations and detergents. The objectives of the research was to determine the characteristics of the protease enzyme produced by strain A1, including incubation time, substrate concentration azokasein, the optimum temperature and pH also stability. The effect of some metal ions as activators or inhibitors of the protease enzyme activity measured with a spectrophotometer at ? 280 nm. Strain A1 was identified by using 16S rDNA sequencing and phylogenetic analysis based on Neighbor Joining method. Strain A1 protease activity was qualitatively demonstrated the presence of a clear zone around the colonies in the medium containing 1% skim milk. Result showed that the highest activity were incubation time of three days, temperature of 50 ºC and pH 8.5 were 87.35 U/mL, 83.44 U/mL and 93.11 U/mL, respectively. Effect of metal ions in the form of divalent and monovalent cations at a concentration of 1 mM on protease A1 activated by divalent cations CaCl2, MnCl2 while divalent cations CuCl2, HgCl2 and monovalent cations KCl, NaCl were inhibitors of each enzyme activity. Result from molecular identification based on 16S rDNA sequence and phylogenetic analysis using Neighbor Joining method suggested that strain A1 was Bacillus subtilis. The strain was registered in the InaCC collection (no. B 398).
SELEKSI, KARAKTERISASI, DAN IDENTIFIKASI BAKTERI PENGHASIL KITINASE YANG DIISOLASI DARI GUNUNG BROMO JAWA TIMUR Soeka, Yati Sudaryati; Sulistiani, Sulistiani
Jurnal Natur Indonesia Vol 13, No 2 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (72.571 KB) | DOI: 10.31258/jnat.13.2.155-161

Abstract

Selection, characterization and identification of bacteria that can produce chitinase enzyme were isolated fromBromo Mountain, East Java. The 48 isolates were tested for capability to degrade chitine qualitatively, semiquantitatively and quantitatively. The result showed that 2 isolates, B2-4 and NA S4-1 could degrade chitin, withactivities of 4.8. 10-3 and 3.1 . 10-3 U/ml, after 1 and 2 days incubation respectively. By using molecular characterizationmethods, partial sequences of 16S rDNA and the primers 9F & 1510R were identified as Stenotrophomonas sp.
MANAJEMEN PENGPROV PERSANI JAWA TENGAH DALAM PERSIAPAN MENGIKUTI PON XVIII TAHUN 2012 DI RIAU Sulistiani, Sulistiani; Soetardji, Soetardji; Junaidi, Said
Journal of Sport Sciences and Fitness Vol 1 No 2
Publisher : Journal of Sport Sciences and Fitness

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Abstract

Immobilization of Lactobacillus plantarum B134 Cells using Sodium Alginate for Lactose Hydrolysis in UHT Milk Hartono, Lusiana Kresnawati; Khusniati, Tatik; Artika, I Made; sulistiani, Sulistiani; Choliq, Abdul
Current Biochemistry Vol 1, No 2 (2014)
Publisher : Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/71-82

Abstract

Hydrolysis of lactose in milk by β-galactosidase from immobilized bacterial cells has the potential to alleviate the problem of lactose intolerance. The present study was aimed to immobilize cells of L. plantarum strain B134 and evaluate their efficiency in hydrolyzing lactose in ultra high temperature (UHT) milk. Immobilized cells were generated by mixing cell suspensions with solutions of sodium alginate and calcium chloride. The β-galactosidase activity of the immobilized cells was tested by determining their ability in hydrolyzing lactose in UHT milk (whole milk and skimmed milk). Results showed that cells of L. plantarum strain B134 were entrapped optimally using a combination of 1 % sodium alginate, 100 mM calcium chloride and 12 % w/v cell suspension. The highest β-galactosidase activity was achieved at pH 6.5 and a temperature of 45 ºC for 5 minutes incubation time. The immobilization efficiency achieved was 28.95 %. The immobilized cells could reduce lactose by up to 85.45 % in UHT whole milk and 91.26 % in UHT skimmed milk. The times required for that reduction of lactose in UHT whole milk and UHT skimmed milk were 12 hours and 9 hours respectively. The immobilized cells could be re-used up to 4 times for efficient lactose hydrolysis for both types of milk. Therefore, immobilized cells of L. plantarum B134 have the potential to be used for lactose hydrolysis in UHT milk.
KUALITAS NUTRISI ANEKA TEPUNG DAN KUE TALAM BERBASIS BAHAN PANGAN PULAU ENGGANO DENGAN PENAMBAHAN Lactobacillus plantarum B110 [Nutrition Quality of Various Flour and Talam Cake Based on Enggano Island Food Material Added with Lactobacillus plantarum B110] Khusniati, Tatik; Sulistiani, Sulistiani; Choliq, Abdul; Nanta, Dhea Loka; Wardani, Dita Kusuma; Saraswati, Dahniar
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2222

Abstract

Nine foodstuffs from Enggano island were processed to flour as wheat flour alternative. To increase flour quality and its derivative product, Lactobacillus-plantarum B110 was added those flour and talam cake made from such flour. They were forest cassava (Dioscorea sp.), ararut sago (Marantha arundinacea), tacca (Tacca Leontopetaloides), egg taro (Alocasia sp.), oil taro (Alocasia sp.), gadung (Dioscorea hispida), gogo rice (Oryza sativa), corn (Zea mays), and belinjo (Gnetum gnemon).  The quality assessment consisted of HCN detection (qualitative), nutrition contents (proximate analysis), acid  (titration method), glucose (GOD-POD kit), and organoleptic tests (20 panelists). The results show that acid and glucose contents of the nine flours increased after L. plantarum B110 addition. Acid content of  the flours was 0.0144-0.2475%, while  glucose was  0.056-0.449%.  Carbohydrate, energy and acid contents of L. plantarum B110 talam cake were higher than control, which those were 34.13%, 190.33 cal/100gr, 0.00082%, while protein, lipid, water, ash were 1.75%, 5.22%, 57.9 %, 1.01%, respectively. The talam cake was accepted by panelists with values: 5.50 (taste), 5.6 (colour), 4.55 (flavour), 4.00 (texture), 4.35 (homogeneous). It was concluded that the flour and talam cake quality increased with addition of L. plantarum B110. 
VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3242.558 KB) | DOI: 10.14203/beritabiologi.v16i2.2772

Abstract

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.
POTENSI ANTIBAKTERI TIGA SPESIES BAKTERI ASAM LAKTAT ASLI ENGGANO TERHADAP BAKTERI PATOGEN DAN PEMBUSUK MAKANAN [Antibacterial Potential of Three Indigenous Lactic Acid Bacteria Species from Enggano Against Pathogenic and Food Spoilage Bacteria] sulistiani, sulistiani; Khusniati, Tatik
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2879.838 KB) | DOI: 10.14203/beritabiologi.v15i3.2410

Abstract

Lactic acid bacteria (LAB) strains have been known for their antimicrobial compounds that are important for food industry,including extension of expired time for food products. In this study, LAB isolates were isolated anaerobically from coconut sap using MRSA medium. Antibacterial analysis was carried out using microdilution method on microplate. The antibacterial assay showed that 85 isolates of LAB exhibit antibacterial activities against Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes, and Bacillus cereus. Molecular identification of 13 physiologically characterized LAB strains based on 16S rDNA sequence determined three species, namely, Leuconostoc mesenteroides (strain EN17-1, EN17-8, EN17-12, EN17-15, EN17-34, EN17-41, EN17-43, EN17-45, EN17-46, EN38-34), Lactobacillus fermentum (strain EN17-2, EN38-44) and L. satsumensis strain EN38-32. The strong and wide broad spectrum antibacterial activity was produced by L. satsumensis strain EN38-32.