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COMPARISON ABILITY OF POLYMERS ACRYCOAT S100 AND HPMC K4M TO ENTRAPMENT EFFICIENCY DOMPERIDONE IN MICROSPHERES Apridamayanti, Pratiwi; Sinaga, Nora Nurlina; Desnita, Rise
Indonesian Journal of Pharmaceutical Science and Technology Vol 7, No 1 (2020)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v7i1.18461

Abstract

Domperidone is a prokinetic and antiemetic agent which has low bioavaibility. To increase the bioavaibility of drug, it can be modified into microsphere that can hold drug more longer in gastric to improve the bioavaibility. The microsphere preparation requires a polymer that can make matrix system to protect and deliver the drugs. Acrycoat S100 and HPMC K4M are the usual polymers that used for encapsulation and  have biodegradable characteristic. The aim of this research is to know the comparison ability of two different polymers to entrapment the drug in microsphere. Microsphere domperidone made by solvent evaporation method in 6 formula. F1, F2 and F3 using 50 mg, 100 mg and 150 mg Acrycoat S100 polymer, while F4, F5 and F6 using 50 mg, 100 mg and 150 mg HPMC K4m polymer. The tests were conducted by the determination of the percentage of entrapment efficiency using UV spectrophotometer and evaluation of organoleptic, particle measurement and surface microsphere morphology. The results showed that F3 with Acrycoat S100 polymer has a greater entrapment efficiency of 78,712% ± 4,260% compared to the highest percentage efficiency of HPMC K4M polymer of 4,734±0,390.Key words: Acrycoat S100, domperidone, entrapment efficiency, HPMC K4M, microsphere
CEMARAN BAKTERI Eschericia coli DALAM BEBERAPA MAKANAN LAUT YANG BEREDAR DI PASAR TRADISIONAL KOTA PONTIANAK Sari, Rafika; Apridamayanti, Pratiwi
Kartika Jurnal Ilmiah Farmasi Vol 2, No 2 (2014)
Publisher : Universitas Jenderal Achmad Yani, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/kjif.v2i2.28

Abstract

Makanan laut merupakan salah satu jenis makanan yang banyak dikonsumsi oleh masyarakat selain sebagai komoditi ekspor. Tingginya kandungan protein dan air serta pH-nya yang mendekati netral menjadikannya media yang cocok untuk pertumbuhan bakteri sehingga menyebabkan makanan laut cepat mengalami proses pembusukan. Mengkonsumsi makanan laut yang telah terkontaminasi bakteri hidup atau toksin yang dihasilkannya dapat menyebabkan keracunan makanan. Tujuan penelitian ini adalah untuk mengetahui adanya kontaminasi bakteri koliform E.coli sebagai indikator pencemaran pada makanan laut dan memberikan informasi kelayakan dan keamanan konsumsi dari makanan laut di dua pasar tradisional terbesar di daerah Pontianak.  Sampel yang digunakan adalah ikan, sotong dan udang. Penelitian terhadap sampel dilakukan menggunakan uji Most Probable Number (MPN) yang dilengkapi dengan uji biokimia untuk mengidentifikasi jenis bakteri pada sampel melalui penanaman bakteri pada media agar Lactose Broth (LB) dan Briliant Green Lactose Bile Broth (BGLB). Hasil penelitian menunjukkan bakteri koliform E.coli terdeteksi pada 100% sampel dengan nilai MPN  yang tidak memenuhi kriteria kelayakan konsumsi, yakni >3/g, dengan nilai paling terbesar dimiliki oleh sampel sotong yang dijual di kedua pasar tradisional, yakni 220/g. Hasil uji biokimia menunjukkan sampel positif mengandung E.coli dengan terbentuknya cincin berwarna merah pada media uji.
Efektivitas SNEDDS Ekstrak Kulit Manggis Terhadap Bakteri P. mirabilis dan S. epidermidis yang Terdapat pada Ulkus Diabetik Sari, Rafika; Pratiwi, Liza; Apridamayanti, Pratiwi
Pharmaceutical Sciences and Research (PSR) Vol 3, No 3 (2016)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.871 KB) | DOI: 10.7454/psr.v3i3.3287

Abstract

Mangosteen rind has the ability to conduct antibacterial activity. Under the development relating to drug delivery system, SNEDDS were known to boost the penetration of active compound. The aim of this research was to compare the effectiveness of the antibacterial activity at both SNEDDS preparation and the ethanol extract of mangosteen rind as the antibacteria against the bacteria that cause diabetic ulcers with the most prevalence whether it was Gram positive or Gram negative bacteria like Proteus mirabilis and Staphylococcus epidermidis. The research started by maceration process with the crude extract as the result continued with the phytochemical screening which subsequently prepared as SNEDDS preparations. The SNEDDS preparation prepared earlier then analyzed to see the content of the active compound using spectrophotometer  UV-Vis  and  the measuring of antibacteria activity with bacteria growth inhibitory parameter at ethanol extract SNEDDS preparations of mangosteen rind then compared to ciprofloxacin and were analyzed the data afterwards using ANOVA. The results shows that SNEDDS preparation of mangosteen extract have an activity against both typed of bacteria that cause diabetic ulcers shows by significant differences between both group which is P. mirabilis bacteria group and S. epidermidis bacteria group in ANOVA test  with significant number of 0,000 and shows significant differences between both group.
Antibiotic Sensitivity in Pseudomonas aeruginosa of Diabetic Patient’s Foot Ulcer Apridamayanti, Pratiwi; Meilinasary, Khairunnisa Azani; Sari, Rafika
Pharmaceutical Sciences and Research (PSR) Vol 3, No 2 (2016)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (15.981 KB) | DOI: 10.7454/psr.v3i2.3289

Abstract

Diabetes Mellitus (DM) patients are at risk to have the diabetic ulcer. The main reason for DM’s patient with ulcer complication to be treated and healed in hospital is bacterial infection. One of many bacteria that infects diabetic ulcer is Pseudomonas aeruginosa. This conditian can be treated by antibiotic. The using antibiotic is often inaccurate causing the microbe resistance. To choose the right antibiotic, it needs to test the antibiotic’s sensitivity towards Pseudomonas aeruginosa. The aimed of this study was to determine the sensitivity of antibiotics against Pseudomonas aeruginosa. Sample was taken from diabetic ulcers swab with grade III and IV Wagner. The identification of bacteria was managed using the biochemical test and Gram staining test. Antibiotic sensitivity was determined by Kirby Bauer method. Antibiotics that were found still sensitive towards Pseudomonas aeruginosa included ciprofloxacin, norfloxacin, imipenem, levofloxacin, meropenem, ceftriaxone, and cefotaxime, whereas cefadroxil and amikacin were resistant. Antibiotics that can be used for Pseudomonas aeruginosa in diabetic foot ulcer patients are ciprofloxacin, norfloxacin, imipenem, levofloxacin, meropenem, ceftriaxone, and cefotaxime.  
Skrining Aktivitas Antibakteri Bakteriosin dari Minuman Ce Hun Tiau Sari, Rafika; Deslianri, Lia; Apridamayanti, Pratiwi
Pharmaceutical Sciences and Research (PSR) Vol 3, No 2 (2016)
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.768 KB) | DOI: 10.7454/psr.v3i2.3272

Abstract

Lactic acid bacteria is one of the beneficial bacteria because it can produce an antimicrobial peptide called bacteriocin. Bacteriocin has been applied as a natural food preservative because it prevents effectively the growth of pathogenic bacteria on food or drink. The aimed of this study was to identify the lactic acid bacteria as producers of bacteriocin from Ce hun tiau which has inhibitory activity againts pathogenic bacteria such as Escherichia coli, Staphylococus aureus and Salmonella typhi. In this study, Lactic acid bacteria were isolated from Ce Hun Tiau using streak plate method on media deman Rogose Sharpe. Screening bacteriocin use disc diffusion method to find out the inhibitory activity of isolates against Escherichia coli, Staphylococus aureus and Salmonella typhi and for confirmation test using proteolytic enzymes. The results showed one isolate of lactid acid bacteria was succesfully isolated from Ce hun tiau, identified as Lactobacillus plantarum. This isolate could inhibit the growth of pathogenic bacteria Escherichia coli, Staphylococus aureus and Salmonella typhi which is a bacteriocin activity.  
Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia Apridamayanti, Pratiwi; Pratiwi, Rarastoeti; Purwestri, Yekti Asih; Sri Tunjung, Woro Anindito; Rumiyati, Rumiyati
Indonesian Journal of Biotechnology Vol 22, No 1 (2017)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (972.35 KB) | DOI: 10.22146/ijbiotech.26401

Abstract

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.
POTENSI SENYAWA ANTIOKSIDAN TANAMAN ENDEMIK PADA MASYARAKAT DAYAK SEKAJANG DI KALIMANTAN BARAT Apridamayanti, Pratiwi; Kurniawan, Hadi
Jurnal Pendidikan Informatika dan Sains Vol. 7 No. 1 (2018): Jurnal Pendidikan Informatika dan Sains
Publisher : IKIP PGRI Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.524 KB) | DOI: 10.31571/saintek.v7i1.770

Abstract

Penelitian ini dilakukan untuk mengetahui khasiat tanaman obat yang digunakan secara empiris pada masyarakat dayak di dusun Sekajang. Kelompok masyarakat adat yang berada di dusun Sekajang yaitu Dayak Semuh. Menurut hasil wawancara yang dilakukan terhadap Batra (Pengobat Tradisional) pada penelitian Riset Tanaman Obat dan Jamu tahun 2015 diperoleh ramuan yang digunakan dalam pengobatan nyeri oleh masyarakat dayak sekajang. Ramuan yang digunakan terdiri  daun buluh (Bambusa vulgaris), daun sak (Melastoma malabathricum), kulit batang langsat (Lansium domesticum correa), daun saoh, daun empedu. Pada penelitian ini proses ekstraksi dilakukan secara maserasi menggunakan etanol 95%. Dilakukan uji metabolit kandungan senyawa kimia dan uji aktivitas antioksidan dengan menggunakan DPPH. Hasil identifikasi terhadap kandungan metabolit sekunder senyawa diperoleh adalah alkaloid, fenol, tannin, flavonoid, saponin, dan terpenoid. Nilai aktivitas antioksidan yang dimiliki masing-masing ekstrak yaitu Daun Buluh (Bambusa vulgaris) 67%, Daun Sak (Melastoma malabathricum) 64%,  Kulit Batang Langsat (Lansium domesticum correa) 55,6%, Daun Empedu 64,98 %, dan Daun Saoh 73,02%.Kata Kunci:  Antioksidan , DPPH, Dusun Sekajang, Tanaman Endemik.
PELATIHAN PEMBUATAN SABUN CAIR LIDAH BUAYA PADA KELOMPOK ASPELIYA PONTIANAK Robiyanto, Robiyanto; Sari, Rafika; Apridamayanti, Pratiwi; Untari, Eka Kartika
GERVASI: Jurnal Pengabdian kepada Masyarakat Vol 1, No 1 (2017): GERVASI: Jurnal Pengabdian Kepada Masyarakat
Publisher : IKIP PGRI Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.571 KB)

Abstract

Kecamatan Pontianak Utara merupakan pusat industri di Kota Pontianak yang salah satunya dikenal sebagai produsen minuman lidah buaya. Hasil samping produk olahan lidah buaya adalah kulit lidah buaya yang sering tidak dimanfaatkan oleh pihak produsen. Limbah kulit lidah buaya diketahui memiliki potensi sebagai antibakteri. Tujuan kegiatan pengabdian adalah untuk memanfaatkan limbah kulit lidah buaya menjadi sabun cair dalam bentuk pelatihan kepada produsen industri rumah tangga lidah buaya yang tergabung dalam kelompok Aspeliya (Asosiasi Pengusaha Lidah Buaya) Kota Pontianak. Formulasi sabun cair lidah buaya terdiri dari infusa kulit lidah buaya 30%, basis terdiri dari 3 minyak (minyak zaitun, minyak kelapa, dan minyak dengan perbandingan 1:1:1), asam stearate 2%, HPMC 3%, gliserin 18,75%, larutan KOH 10%, BHT 0,02%, dan akuades ad 100 ml. Uji pH, uji kadar asam lemak bebas dan alkali bebas, uji organoleptik, tinggi busa, viskositas serta bobot jenis dilakukan setelahnya. Saat pelatihan, peserta diminta untuk praktik langsung membuat sabun cair dengan bimbingan dari narasumber. Pelatihan diakhiri dengan sesi tanya jawab dan penyebaran kuesioner. Kesimpulan dari kegiatan pengabdian yaitu seluruh peserta merasakan manfaat dan termotivasi dengan adanya pelatihan. Para peserta juga berharap untuk mendapatkan pelatihan produk olahan lainnya untuk menambah pengetahuan. Kata Kunci: sabun cair, lidah buaya. AbstractPontianak Utara District is industrial centres of Pontianak city, one of them is known as aloe vera drink producing centre. Waste of the aloe vera drink product is the leave skin which is unused by producer. The aloe vera leave skin has been reported to have antibacterial effect. The purpose of this community service activity was to utilize unused aloe vera leave skin to be liquid soap by giving a workshop to home industry of aloe vera producers whom are members of Aspeliya group in Pontianak. The formulation of aloe vera liquid soap consisted of skin infusum 30%, bases (olive oil, coconut oil, and castor oil = 1:1:1), stearic acid 2%, HPMC 3%, glycerin 18,75%, KOH soln 10%, BHT 0,02%, and aquadest ad 100 ml. Acidity test (pH), free fatty acid level and free alkaline test, organoleptic test, foaming height, viscosity and density test were also conducted. During the workshop, participants were asked to practice making liquid soap with tutorial from the speaker.This workshop was finished with Q&A session and questionnaire distribution. The conclusion of this workshop was all participants feltthe benefit and motivated.They also expected to join another similar workshop to make other processed products to enrich their knowledge. Keywords:liquid soap, aloe vera.
Penetrasi Natrium Askorbil Fosfat dalam Sistem Niosom Span 40 secara In Vitro DESNITA, RISE; LESTIAWATI, VENI; APRIDAMAYANTI, PRATIWI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 2 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1381.569 KB)

Abstract

Sodium ascorbyl phosphate is a hydrophilic compound that is difficult to pass through the stratum corneum. One of the efforts to increase the penetration of the drug through the stratum corneum is to develop in niosome system. This research aims to determine the optimal concentration of span 40 to improve the entrapment efficiency of niosome sodium ascorbyl phosphate formulated in gel and determine the stability and penetration ability of gel formulas. Concentrations of span 40 were varied into three formulas: F1 (100 μmol), F2 (150 μmol) and F3 (200 μmol). Gel formulation was made in two formulas using gel base viscolam MAC 10: G1 (sodium ascorbyl phosphate in niosome system) and G2 (sodium ascorbyl phosphate without niosome system). The stability tests performed for 28 days include organoleptic, pH and determination levels of sodium ascorbyl phosphate in the gel. Penetration test was performed by in-vitro test using shed snake skin membrane. The result showed the optimum entrapment efficiency of F1 formula is 99.13±0.10%. G1 formula is stable for 28 days of storage than G2 formula. The use of span 40 as niosome composer can increase the penetration of sodium ascorbyl phosphate in the gel formulation were 89.04±0.01%.
PENENTUAN NILAI MIC EKSTRAK ETANOL KULIT LIDAH BUAYA (Aloe vera Linn) TERHADAP ISOLAT BAKTERI Pseudomonas aeruginosa RESISTEN ANTIBIOTIK Sari, Novita; Apridamayanti, Pratiwi; Sari, Rafika
Jurnal Pendidikan Informatika dan Sains Vol. 7 No. 2 (2018): Jurnal Pendidikan Informatika dan Sains
Publisher : IKIP PGRI Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.203 KB) | DOI: 10.31571/saintek.v7i2.1062

Abstract

Resistensi merupakan kegagalan pengobatan suatu antibiotika dengan dosis terapi. Pseudomonas aeruginosa diketahui resisten terhadap beberapa antibiotik. Selain antibiotik, bahan alam dapat digunakan sebagai antibakteri dengan pemanfaatan kulit lidah buaya karena mengandung senyawa antrakuinon yang berpotensi sebagai antibakteri. Penelitian bertujuan mengetahui nilai Minimum Inhibitory Concentration (MIC) dari ekstrak etanol kulit lidah buaya terhadap bakteri Pseudomonas aeruginosa. Metode uji yang digunakan adalah metode disc diffusion (Kirby Bauer) dan menggunakan simplisia kulit lidah buaya dimaserasi dengan etanol 96% sehingga didapat ekstrak kental. Ekstrak etanol kulit lidah buaya dibuat konsentrasi larutan uji 10; 9,75; 9,5; 9; 8,75; 8,5; 8,25; 8; 7,75; 7,5; 5; 2,5 dan 1%. Kontrol negatif menggunakan metanol pa. Uji KLT untuk mengetahui kandungan senyawa pada ekstrak etanol kulit lidah buaya, dengan fase diam silika gel GF254 dan fase gerak etil asetat:metanol:air (10:0,7:0,3). Hasil penelitian ini menunjukkan zona hambat minimum ialah pada konsentrasi 8,5% (6mm). Dapat disimpulkan bahwa ekstrak etanol kulit lidah buaya mempunyai efek sebagai antibakteri terhadap Pseudomonas aeruginosa.