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Kadar Logam Timbal (Pb) dalam Darah Penjual Klepon Kustiningsih, Yayuk; Fitriyanti, Noni; Nurlailah, Nurlailah
Medical Laboratory Technology Journal Vol 3, No 2 (2017): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (758.942 KB) | DOI: 10.31964/mltj.v3i2.168

Abstract

A kelepon seller is one of the groups susceptible to metal pollution from motor vehicle emissions from the tetraethyl-Pb and tetramethyl-Pb combustion products that are always added to motor vehicle fuel. Lead metal has a negative impact on the environment including human health. The first effect of chronic Pb poisoning before reaching the target organ is the presence of hemoglobin synthesis disorder so that the hemoglobin level decreases.The effect of lead poisoning in the blood can occur if the Pb content is more 70 ug/dl or 0.7 ppm which will lead to anemia. This study aims to determine a level of lead metal in the blood of kelepon seller that exceeds the threshold. Descriptive survey research method with cross-sectional design on the sample from 15 respondents. The lead metal (Pb) in the blood is measured using an Atomic Absorption Spectrophotometer (SSA) device. The result of the research shows that there are 13 respondents (86,7%) whose Pb metal content exceeds the blood lead threshold that is 20 ug / dL or 0,20 ppm. The highest level of lead in the blood of the respondents was 0.73 ppm and the lowest was 0.10 ppm. Two respondents with lead in blood less than 0.20 ppm are known to always use personal protective equipment while working, even though the working time is 6-10 hours/day. It is advisable to conduct further research by examining Hb levels and abnormalities of erythrocytes in the blood of the kelepon seller and conducting similar research
Potention of Active Charcoal from Musa Paradisiaca and Manihot Utilissima Shell in Degrading River Contamination Nurlailah, Nurlailah; Thuraidah, Anny; Kustiningsih, Yayuk
Medical Laboratory Technology Journal Vol 4, No 1 (2018): period Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.29 KB) | DOI: 10.31964/mltj.v4i1.180

Abstract

River water is one of type water surface which is a lot of finding in South Kalimantan. Generally, this water used by the citizen for cooking and bathing. But along with era growth, this river becomes as disposal of various industrial waste. The contamination of heavy metal like Fe, Mn, Pb, Cd, and turbidity enhance progressively and degrade the water quality. Some natural substance which can be used to improve river water quality is active charcoal from Musa paradisiaca and Manihot utilissima shell which is easy to get. Target research is determined to find optimum dose of active charcoal from Musa paradisiaca and Manihot utilissima shell that capable to degrade heavy metal contamination and turbidity in water river. It used experimentally with pretest and post-test with control group design. A result of research showed the optimum dose of active charcoal Musa paradisiaca shell was 15 gram , it could absorb Pb 28,8% and Mn 24,6%, but 10 gram can degrade turbidity until 83,8 %, while the optimum dose of Manihot utilissima shell to 25,4% Pb and degraded 77,5% of turbidity was 15 gram. Statistical test result with Kruskal Wallis got the p-value less than 0,05 it meant there was a difference between the treatment of active charcoal from Musa paradisiaca and Manihot utilissima shell in degrading the contamination. A conclusion is those active charcoal having a potential to enhance the water river quality. Suggested to use another activator to the potential like stirring, time of contact and the different mass.
Efektivitas Air Rebusan Daun Binahong (Anredera cordifolia) Terhadap Pertumbuhan Salmonella typhi Dwiyanti, Ratih Dewi; Nurlailah, Nurlailah; Widiningsih, Indah Kurnia
Medical Laboratory Technology Journal Vol 1, No 1 (2015): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (592.498 KB) | DOI: 10.31964/mltj.v1i1.7

Abstract

Typhus is one of acute febrile illness caused by the bacterium Salmonella typhi. Treatment of typhoid fever usually use antibiotics, the use of antibiotics can cause side effects. People today are using treatment with natural ingredients, one of which is Binahong (Anredera cordifolia) compounds containing alkaloids, polyphenols, flavonoids, saponin, and anthraquinone is efficacious as an antibacterial. This study aims to determine the effectiveness of the water decoction of leaves Binahong against Salmonella typhi growth in vitro. This type of research is true experiment with posttest study design Only Control Group Design and methods used are diffusion (wells) with 5 treatment. The concentration of the cooking water leaves the dgunakan Binahong is 20%, 40%, 60%, 80% and 100%. The result showed inhibition zone water decoction of the leaves Binahong against Salmonella typhi at a concentration of 20%, 40%, 60%, 80% is 0 mm, whereas at 100% concentration obtained inhibition zone of 11 mm. It is concluded that the water decoction of the leaves Binahong at a concentration of 100% has the ability to inhibit the growth of Salmonella typhi, but these results have not been effective because it is still in the category of resistance. It is suggested for further research to increase the concentration of water decoction of the leaves binahong or use alcohol extract of leaves binahong to inhibit the growth of Salmonella typhi. Keywords: Water decoction of leaves Binahong, Salmonella typhi, antibacterial. Abstrak: Penyakit tifus atau dikenal dengan demam tifoid atau demam enterik adalah salah satu penyakit demam akut yang disebabkan oleh bakteri Salmonella typhi. Pengobatan demam tifoid biasanya menggunakan antibiotik, penggunaan antibiotik dapat menimbulkan efek samping. Masyarakat saat ini banyak menggunakan pengobatan dengan bahan alami, salah satunya adalah Binahong (Anredera cordifolia) yang mengandung senyawa Alkaloid, Polifenol, Flavonoid, Saponin, dan Antrakuinon yang berkhasiat sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui efektifitas air rebusan daun Binahong terhadap pertumbuhan Salmonella typhi secara in vitro. Jenis penelitian ini adalah true eksperiment dengan rancangan penelitian Posttest Only Control Group Design dan metode yang digunakan adalah difusi (sumuran) dengan 5 perlakuan. Konsentrasi air rebusan daun Binahong yang dgunakan adalah 20%, 40%, 60%, 80% dan 100%. Hasil penelitian didapatkan zona hambat air rebusan daun Binahong terhadap Salmonella typhi pada konsentrasi 20%, 40%, 60%, 80% adalah 0 mm, sedangkan pada konsentrasi 100% didapatkan zona hambat sebesar 11 mm. Kesimpulan penelitian ini adalah air rebusan daun Binahong pada konsentrasi 100% memiliki kemampuan menghambat pertumbuhan Salmonella typhi, namun hasil ini belum efektif karena masih dalam kategori resisten. Disarankan bagi peneliti selanjutnya untuk meningkatkan konsentrasi air rebusan daun binahong atau menggunakan ektrak alkohol daun binahong untuk menghambat pertumbuhan Salmonella typhi. Kata kunci : Air rebusan daun Binahong, Salmonella typhi, antibakteri.
Analisis Kadar Siklamat pada Es Krim di Kota Banjarbaru Nurlailah, Nurlailah; Alma, Nurhayati Aslami; Oktiyani, Neni
Medical Laboratory Technology Journal Vol 3, No 1 (2017): periode Juni
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.806 KB) | DOI: 10.31964/mltj.v3i1.148

Abstract

Sweeteners are one of the components which are often added to foodstuffs. Synthetic sweeteners are widely circulated in the community is cyclamate. Consumption of cyclamate that exceeds the dose will lead to bladder cancer. It will also cause lung, liver, and lymph tumors. The purpose of this study was to determine the presence of cyclamate in ice cream that exceeded the required threshold. The type of this research is the descriptive survey. The sample of this research is ice cream that produced by household companies from all ice cream traders in North Banjarbaru with 11 ice cream sellers. The variables in this study were cyclamate content found in ice cream. The results showed that 11 samples of ice cream were examined, 9 samples containing cyclamate with the highest level of 7.37 g / kg as cyclamic acid. The conclusion of this study is ice cream containing cyclamate found as much as 82% (9 samples), while 18% (2 samples) others negative, from 9 samples containing cyclamate positive, 89% did not qualify the requirements of PERMENKES Number 208 in 1985 that exceeds 2 gr/Kg as cyclamate acid. More specific checks are required for the analysis of cyclamate levels by other methods such as High Performance Liquid Chromatography.
Pemanfaatan Arang Aktif Dalam Menurunkan Logam Berat Terlarut(Pb)Padalimbahlaboratorium haitami, Haitami; muslim, Muhammad; nurlailah, Nurlailah
Jurnal Skala Kesehatan Vol 7 No 1 (2016): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.893 KB)

Abstract

Laboratorium kimia pada pendidikan tenaga kesehatan merupakan sarana yang berperan penting dalam meningkatkan kualitas dan kuantitas pelaksanaan tridharma perguruan tinggi civitas akademika. Tingkat penggunaan laboratorium tersebut sangat tinggi tergantung aktifitas dari pendidikan tinggi tersebut. Laboratorium pendidikan tenaga kesehatan seperti program studi Analis Kesehatan, Gizi dan Kesehatan Lingkungan Poltekkes Kemenkes Banjarmasin banyak mengandung logam berat dan bahan-bahan berbahaya lainnya dalam aktifitas praktikum mahasiswa, sehingga menghasilkan limbah B3. Tujuan dari penelitian ini adalah Untuk mengetahui variasi konsentrasi, lama waktu dan kecepatan pengadukan arang aktif. Hipotesis dalam penelitian ini adalah konsentrasi arang aktif, lama waktu dan kecepatan pengadukan mampu menurunkan kadar timbal dalam limbah laboratorium. Jenis penelitian yang digunakan adalah true eksperiment dengan menggunakan rancangan pretest and post test with control group design. Bahan penelitian yang digunakan adalah arang aktif komersial yang digunakan untuk pengolahan limbah kimia sisa hasil kegiatan praktikum mahasiswa.Data yang diperoleh dari pengukuran Pb berskala ukur ratio, dianalisa secara deskriptif dengan menghitung prosentasi rata-rata penurunan kadar Pb pada setiap varian. Untuk mengetahui kemampuan tiap varian ditentukan dengan menghitung prosentasi optimum dari tiap varian, di antaranya berapa variasi konsentrasi arang aktif. Hasil penelitian pengolahan limbah laboratorium dengan menggunakan arang aktif diperoleh konsentrasi optimum arang aktif adalah 25,0 g, dengan lama pengadukan selama 45 menit pada kecepatan pengadukan 150 rpm. Yang dapat menurunkan kadar Pb sampai 89,24%.  Dari hasil penelitian yang telah dilakukan dapat disarankan bahwa limbah laboratorium sisa hasil kegiatan praktikum, sebelum dibuang kelingkungan sebaiknya diolah dulu dengan menggunakan arang aktif. Untuk penelitian selanjutnya dapat melakukan penelitian dengan menggunakan arang aktif yang telah diaktivasi, misalnya dengan menggunakan asam dan lain sebagainya. Kata Kunci: Logam Berat, Arang Aktif
Kandungan Asam Oksalat Sayur Bayam Fitriani, Herlena; Nurlailah, Nurlailah; Rakhmina, Dinna
Medical Laboratory Technology Journal Vol 2, No 2 (2016): periode Desember
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (673.028 KB) | DOI: 10.31964/mltj.v2i2.95

Abstract

Spinach is one of vegetable that is often used as processed food by the people of Indonesia. Besides containing many nutrients, spinach also contains chemical compounds that are negative, that is oxalic acid. Oxalic acid and its salts are water soluble that can be harmful because these compounds are toxic. This study aimed to determine differences in levels of oxalic acid in spinach when the water is allowed to stand at room temperature. The type of research was pre-experiment with one group pretest-posttest design. Samples of spinach water were divided into 4 treatment and the level of oxalic acid was examined by using permanganometry titration method. Results of research on each treatment showed oxalic acid levels on 0 hour standing was 3753.2 mg/L, 2 hours standing was 3980.0 mg/L, 4 hours standing was 4066.5 mg/L, and the 6 hours standing was 4254.5 mg/L. Repeated ANOVA statistical test results stated there were significant differences in the levels of oxalic acid in spinach water between 0 hour standing and room temperature-standing with a significance value of p
Relationship Between Gender, Age, Duration And Frequency Of Hemodialysis Therapy With The Creatinine Level reduction Of Pre And Post Hemodialysis Thuraidah, Anny; Misbawati, Misbawati; Nurlailah, Nurlailah; Haitami, Haitami
Medical Laboratory Technology Journal Vol 5, No 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.302 KB) | DOI: 10.31964/mltj.v5i1.222

Abstract

The kidneys are organs of the body that function to regulate water balance in the body, the concentration of electrolytes in the blood and acid-base balance and secretion of waste material. If the kidney fails to function, the patient will need immediate treatment and even undergo hemodialysis (HD) therapy. A critical indicator in determining whether a person with impaired kidney function requires HD therapy is to know the creatinine level. The study aimed to ascertain the differences in creatinine levels pre and post HD also study the relationship between the age, gender, duration and frequency of HD therapy of respondents with the decrease of creatinine levels pre and post. Type of research detailed survey with a cross-sectional design. The sample was taken using a total sampling technique of 35 respondents from H BadaruddinKasimHospitalin Tanjung with a sample examination technique using the Jaffe method. The examination results of creatinine levels average for pre and post hemodialysis was11,36 and 5,58 mg/dl, which decreased 51%. The analysis statistically used Paired T-Test has a p-value = 0,000, indicating a significant difference for creatinine levels pre and post HD. Relationship between Age, Gender, Duration, and Frequency of HD Therapy with the decrease of creatinine levels pre and post HD was analyzed with Spearmen correlation and had p values more than 0,05. It means there was no relation between them. Conclusion there were significant differences in creatinine levels reduction pre and post HD while the relationship between the four of respondent characteristics to the magnitude of creatinine reduction show that there was no significant relationship.
BIOLARVASIDES OF CANANGA ODORATA FLOWER AND ZYZYGIUM POLYANTHUM LEAVES ETHANOL EXTRACT ON AEDES AEGYPTI LARVA Nurlailah, Nurlailah; Thuraidah, Anny
Medical Laboratory Technology Journal Online First Article
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v1i1.304

Abstract

Dengue hemorrhagic fever (DHF) is one of the diseases caused by Aedes Aegypti mosquitoes. DHF is still a public health problem, including in Indonesia, due to the high mortality rate caused by this mosquito. The risk caused by the bite of the Aedes sp mosquito is quite dangerous, including acute fever, bleeding, shock, and even death, so that efforts are needed to control it. One of the natural ingredients that can use to eradicate Aedes Aegypti mosquitoes without causing negative impacts on the environment is the Cananga flower and Zyzigium Leaves. This study purposes of determining the optimum concentration of Cananga Flowers and Zyzigium Leaves Ethanol extract of which was having biolarvaside effect to Aedes Aegypti larvae. The method used a simple experiment with Posttest Only With Control Group Design. The data result of the research analyzed with Logit and Wilcoxon test. Achievement of those tests was LC50 Zyzigium leaf extract on contact for 24 hours was 0.609-0.935, and LC99 was between 1.064-1.973 while Cananga flower extract obtained LC50 of 0.228-0.261 and the LC99 was 0.446 to 0.558. Wilcoxon test for compared bioalarvaside effect results between Cananga Flower and Zyzigium Leaf received a significance value less than ? (0.001
CITRUS HYSTRIX D.C JUICE INHIBITS THE GROWTH OF STAPHYLOCOCCUS AUREUS Kusumawardhani, Novina; Thuraidah, Anny; Nurlailah, Nurlailah
Tropical Health and Medical Research Vol 2 No 1 (2020): Tropical Health And Medical Research
Publisher : Baiman Bauntung Batuah Center

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35916/thmr.v0i0.17

Abstract

Citrus hystrix D.C. is a citrus-type citrus tribal plant, one of the most favored and developed fruits by the people of South Kalimantan Indonesia. Citrus hystrix D.C. contains useful chemical compounds, one of which is flavonoids, which function as antibacterial and play a critical role in inhibiting bacterial growth. The purpose of this study was to determine the antibacterial activity of Citrus hystrix D.C. juice at 25%, 50%, 75%, and 100% concentration on the growth of Staphylococcus aureus. This research is experimental with Posttest Only Control Group Design. The material used was local Citrus hystrix D.C., fresh and clean green, obtained from the Astambul area, Banjar Regency, South Kalimantan Indonesia. The results showed that there was a zone of inhibition of Citrus hystrix D.C. juice on Staphylococcus aureus growth which began at 25%, 50%, 75% and 100% concentrations of 13.75mm, 17.25mm, 19.75mm, and 21.75mm, so it can conclude that the juice of Citrus hystrix D.C. influences the growth of Staphylococcus aureus which has the largest inhibitory zone at a concentration of 100%.