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Optimalization of DNA isolation from oral epithelial mucous cell with smear method Nasroen, Saskia L.; Maskoen, Ani Melani; Nurwiadh, Agus
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.335 KB) | DOI: 10.24198/pjd.vol21no3.14110

Abstract

Deoxyribonucleic acid (DNA) is a genetic material which is found in all living organisms. On the human cell or eukaryotes cell, the DNA is found in the nucleus cell and the mitochondria. The DNA arrangement on each cell in human body is the same, that is why, for the analysis meaning, DNA can be isolated from any cell in the body. The source of DNA to be analyzed usually coming from the blood sample by an injection method, such a way resulting in pain and bringing about constraint. Therefore, a study was carried out to look for an alternative of DNA isolation. The aim of this experimental study was to get an optimal DNA isolation method by using oral mucous smear method with a purpose to get a quick and easy DNA isolation. The investigation materials were in the form of samples which were taken from the oral epithelial mucous cells out of three different subjects. The epithelial cells were obtained by the oral mucous smear method which in a variation of two, four and six times of smear applications, respectively. The DNA was then isolated using buffer extraction method. The concentrations of DNA were measured by using ultraviolet spectrophotometer at 260 nm wavelength. The results of DNA isolation were analyzed by Polymerase Chain Reaction (PCR) technique. The optimal DNA isolation could be analyzed by PCR technique. The experimental results show that from three different subjects of study, DNA can be isolated optimally by oral mucous smear method with six times of smear applications.
Hemimandibulectomy of mandibular large odontogenic myxofibroma in adolescence girl and immediate reconstruction with AO plate Pamolango, Victor T.; Nurwiadh, Agus; Sylvyana, Melita; Kasim, Alwin
Journal of Dentomaxillofacial Science Vol 1, No 3 (2016): (Available online: 1 December 2016)
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v1i3.319

Abstract

Odontogenic myxofibroma is a rare, benign, but locally aggressive neoplasm found exclusively in the jaws. It has a predilection to occurs in the 2nd–3rd decade. The lesion often grows without symptoms and presents as a painless swelling. Most common clinical variant is associated with the impacted tooth and shows local invasion. The surgical treatment of these tumors consists of complete enucleation or radical excision. 15th year old girl with a slow growing, painless swelling in the left mandibular region since 2 years. Results of a radiological examination revealed a multilocular radiolucency extending from first molar up to coronoid process with impacted 3rd molar. Biopsy was done, and the histopathological examination showed a myxofibroma. Hemimandibulectomy was done and the tumor mass was resected along with it. Then the mandibular was reconstructed with AO plate. In this case hemimandibulectomy was done due to high rate of recurrence, surgical treatment through bone resection is the most indicated treatment modality and the patient must be followed‑up closely for years. AO Plate for mandibular reconstruction was considered because it was safe, rapid, easy and tissue tolerance is good. The neoplasm diagnosed on the basis of radiographic imaging modalities and histopathological examination.
Sepsis-induced acute kidney injury in oral and maxillofacial infection with upper airway obstruction due to retropharyngeal abscess a case report Kurniawan, Irsan; Nurwiadh, Agus; Lukman, Kiki
Journal of Dentomaxillofacial Science Vol 1, No 3 (2016): (Available online: 1 December 2016)
Publisher : DiscoverSys Inc

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v1i3.318

Abstract

Left untreated or not properly managed odontogenic infection can cause spreading into facial spaces. Severe oral and maxillofacial infection can spread systemically and cause sepsis.1 Sepsis can induce unfavorable condition for the kidney, which is Acute Kidney Injury (AKI).2,3 The aim of this paper is to report a rare case of an oral and maxillofacial  infection with sepsis-induced AKI and upper airway obstruction which have high mortality rate. The standard treatment consists of proper monitoring of vital function, fluid resuscitation, drainage of accumulated purulence, empirical use of antibiotics chosen to cover the spectrum of potential pathogens until culture results are available, and supplemental oxygen.1,4 This is a case report of  a 48 year old female patient with oral and maxillofacial infection suffers sepsis-induced AKI and upper airway  obstruction. This patient was diagnosed as right submandibular abscess  which has extended into right buccal space, with sepsis, stage I AKI, and upper airway obstruction due to retropharyngeal abscess. We managed this patient with oxygenation, fluid resuscitation, antibiotics and analgesic administration, incision and drainage, and also extraction of mandibular right third molar. Tracheostomy, incision and drainage of retropharyngeal abscess were supposed to be performed by ENT department, but the patient refused. However the patient still survived. In case of oral and maxillofacial infection, the involvement of distant organ should be investigated, because this conditions result in high mortality rate. Early diagnosis and prompt treatment are important and able to lead to a better survival. 
Description of odontogenic infection cases at the Oral Surgery Polyclinic of Hasan Sadikin Hospital Bandung, Indonesia Savitri, Ravitha; Karasutisna, Tis; Nurwiadh, Agus
Padjadjaran Journal of Dentistry Vol 24, No 1 (2012): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.558 KB) | DOI: 10.24198/pjd.vol24no1.15382

Abstract

One of the most difficult cases to be managed in dentistry is an odontogenic infection. The study was aimed to finding the description of the odontogenic infection cases at the Oral Surgery Polyclinic of Hasan Sadikin Hospital, Bandung, Indonesia, according to the odontogenic infection types, genders, ages, sources of infection, and type of treatment given. This study was a retrospective-descriptive study with a survey method conducted from January 2009 to December 2010. The results of this study showed that there were total 8 types of odontogenic infection cases. The periapical abscess was the most frequent abscess found in 89 patients (30.6%). Male patients were having more often cases than women with the ratio of 53:47. The most frequent age group was the 21-30 years old age group in 57 patients (22.7%). The highest number of patients from 8 different cases of odontogenic infection (30.2%). The source of odontogenic infection was found most commonly by the mandibular first molar teeth, with as much as 26.6%.
Optimalization of DNA isolation from oral epithelial mucous cell with smear method Nasroen, Saskia L.; Maskoen, Ani Melani; Nurwiadh, Agus
Padjadjaran Journal of Dentistry Vol 21, No 3 (2009): November
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (644.335 KB) | DOI: 10.24198/pjd.vol21no3.14110

Abstract

Deoxyribonucleic acid (DNA) is a genetic material which is found in all living organisms. On the human cell or eukaryotes cell, the DNA is found in the nucleus cell and the mitochondria. The DNA arrangement on each cell in human body is the same, that is why, for the analysis meaning, DNA can be isolated from any cell in the body. The source of DNA to be analyzed usually coming from the blood sample by an injection method, such a way resulting in pain and bringing about constraint. Therefore, a study was carried out to look for an alternative of DNA isolation. The aim of this experimental study was to get an optimal DNA isolation method by using oral mucous smear method with a purpose to get a quick and easy DNA isolation. The investigation materials were in the form of samples which were taken from the oral epithelial mucous cells out of three different subjects. The epithelial cells were obtained by the oral mucous smear method which in a variation of two, four and six times of smear applications, respectively. The DNA was then isolated using buffer extraction method. The concentrations of DNA were measured by using ultraviolet spectrophotometer at 260 nm wavelength. The results of DNA isolation were analyzed by Polymerase Chain Reaction (PCR) technique. The optimal DNA isolation could be analyzed by PCR technique. The experimental results show that from three different subjects of study, DNA can be isolated optimally by oral mucous smear method with six times of smear applications.
Description of odontogenic infection cases at the Oral Surgery Polyclinic of Hasan Sadikin Hospital Bandung, Indonesia Savitri, Ravitha; Karasutisna, Tis; Nurwiadh, Agus
Padjadjaran Journal of Dentistry Vol 24, No 1 (2012): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.558 KB) | DOI: 10.24198/pjd.vol24no1.15382

Abstract

One of the most difficult cases to be managed in dentistry is an odontogenic infection. The study was aimed to finding the description of the odontogenic infection cases at the Oral Surgery Polyclinic of Hasan Sadikin Hospital, Bandung, Indonesia, according to the odontogenic infection types, genders, ages, sources of infection, and type of treatment given. This study was a retrospective-descriptive study with a survey method conducted from January 2009 to December 2010. The results of this study showed that there were total 8 types of odontogenic infection cases. The periapical abscess was the most frequent abscess found in 89 patients (30.6%). Male patients were having more often cases than women with the ratio of 53:47. The most frequent age group was the 21-30 years old age group in 57 patients (22.7%). The highest number of patients from 8 different cases of odontogenic infection (30.2%). The source of odontogenic infection was found most commonly by the mandibular first molar teeth, with as much as 26.6%.
Hemimandibulectomy and intermaxillary fixation: surgical treatment of ameloblastoma in mandible: a case report Sandiah, Jihad H.; Priyanto, Winarno; Adiantoro, Seto; Nurwiadh, Agus
Journal of Case Reports in Dental Medicine Vol 1, No 3 (2019)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5420.54 KB) | DOI: 10.20956/jcrdm.v1i3.101

Abstract

Objective: Ameloblastoma is a tumor derived from epithelial, gingival mucosa or gingivomaxillary that appear on a teeth. It is a benign yet destructive tumor with high recurrence rate. Hemimandibulectomy is one of therapies selected when the pathologic lesion had involved the coronoid processus and the condyle, although the therapy could results in facial defects and mandibular deviation.The purpose of this article is to report a hemimandibulectomy with bridging plate reconstruction and inter-maxillary fixation as a therapy for left mandibular ameloblastoma performed to reduce deviation and facial defects.Method: Fourty-two years old female patient came to the Oral and Maxillofacial Surgery Clinic at Hasan Sadikin Hospital Bandung, with a lump at the left side of lower jaw, it happened since 3 years ago, with small size in the beginning and grow gradually, painless, hard on palpation, and same colored with surrounding tissue.Result: The histopathologic examination results was plexiform type ameloblastoma at left mandible. Hemimandibulectomy is the removal of most or half of the mandible including the entire condyloideus process, coronoid processus, ramus and some mandibular corpus on one side of the jaw. Ameloblastoma is the most common odontogenic tumor in the mandible and maxilla. Ameloblastoma has several variations of histopathologic appearance, but the most commonly seen is the follicular and plexiform type. In most cases, ameloblastoma is usually asymptomatic, growing slowly, and can expand in the affected jaw.Conclusion: Hemimandibulectomy performed to remove pathologic lesions radically to prevent recurrence. Bridging plate is an act used as a mandibular reconstruction. Intermaxillary fixation is one of the ways to reduce mandibular deviation after hemimandibulectomy.Keywords: hemimandibulectomy, intermaxillary fixation, ameloblastoma