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PENGAMATAN BIOAKUSTIK PERGERAKAN IKAN SIDAT ANGUILLA SP. DALAM KONDISI TERKONTROL (AKUARIUM) Pujiyati, Sri; Retnoaji, Bambang; Hananya, Ariel; Lubis, M. Zainuddin
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 10 No. 2 (2018): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.365 KB) | DOI: 10.29244/jitkt.v10i2.21276

Abstract

Pergerakan Ikan sidat menghasilkan suara yang disebut suara hidrodinamik yang merupakan suara atau getaran yang dihasilkan dari sebuah perubahan di medium karena pergerakan ikan. Intensitas suara hidrodinamik terjadi saat arah dan kecepatan renang ikan berubah secara cepat. Pembelokan posisi kepala akan menyebabkan perpindahan medium yang kuat.  Perpindahan tersebut menyebabkan perubahan gelombang tekanan pada medium, yang dapat terdeteksi sebagai suara oleh hydrophone. Penelitian ini bertujuan untuk menganalisis perbedaan suara ikan sidat fase elver dan fase yellow dalam kondisi terkontrol. Penelitian dilakukan di Laboratorium histologi dan embriologi Fakultas Biologi, Universitas Gadjah Mada. Waktu penelitiannya adalah 4-7 Agustus 2016. Alat yang digunakan dalam penelitian ini adalah aquarium kaca, headphone, hidrophone, Seaphone, baterai alkaline kotak, laptop. Bahan yang digunakan masing-masing 5 ekor sidat fase yellow dan fase elver.  Hasil penelitian menunjukkan bahwa Aktifitas ikan sidat fase elver dan fase yellow sangat beragam ditinjau dari  durasi suara ikan sidat saat pagi,siang dan malam.  Pola suara yang terekam berkisar dari frekuensi dari 0 sampai 22.03 KHz. Frekuensi suara ikan sidat dominan dikisaran 151 - 450 Hz, dan memiliki pola suara yang berbeda-beda untuk pagi, siang dan sore. Kesimpulan menunjukkan bahwa durasi dan pola suara hidrodinamika ikan  sidat fase elver maupun fase yellow berbeda nyata antara pagi - siang, juga siang ? sore, namun untuk pagi - sore tidak berbeda nyata.
Effects of Metaldehyde and Niclosamide on The Behavior and Reproductive Capacity of Radix Quadrasi Von Moellendorf (Basommatophora: Lymnaeidae) Retnoaji, Bambang
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 2 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12230

Abstract

The effects of molluscicides exposure, metaldehyde and niclosamide, on the behavior and reproductive capacity of Radix quadrasi was studied under laboratory conditions. R quadrasi were exposed to different concentration-rates of niclosamide: 0, 0.045, 0.058 and 0.075 mg/L, and of metaldehyde: 0, 100, 112 and 130 mg/L. The symptoms of poisoning of treated snails were observed closely throughout the duration of the experiment. Observations were made three times: at the beginning of treatment (0 hour), 12 hours, and 24 hours after exposure. The surviving R. quadrasi were maintained for one month to determine their reproductive capacity. Results indicated that there was immediate reaction of the snails to molluscicide exposure. Lower concentration-rates of molluscicide triggered water-leaving behavior. Such behavior occurred in all of the low and medium concentration-rates of niclosamide and metaldehyde, as well as in some snails from the control group. By contrast, higher concentration-rates caused almost instantaneous immobility. Niclosamide and metaldehyde also significantly reduced the reproductive capacity of R. quadrasi. The number of egg masses and eggs laid varied with the treatments. However, the incubation period of the treated eggs was not statistically different from the treated ones. This study showed that both niclosamide and metaldehyde significantly affected the behavior and reproductive capacity of the R. quadrasi. Between the two molluscicides used in this experiment, niclosamide was more toxic than metaldehyde.
Indeks Gonadosomatik dan Struktur Histologis Gonad Ikan Uceng (Nemacheilus fasciatus, Valenciennes in Cuvier and Valenciennes, 1846) Nurhidayat, Luthfi; Arfiani, Febrina Nanda; Retnoaji, Bambang
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 34, No 2 (2017)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2017.34.2.456

Abstract

Uceng fish is wild native fish of Indonesia that live in fresh water river. It is very common that people consume the fish for it high protein contain. High demand of fish availability cause the fish exploitation increasing rapidly in nature. The high intensity of fish exploitation, soon will induce declining population of the fish and leading to extinction. Uceng fish cultivation is one alternative to solve the tread. The purpose of this research is to examine the Gonadosomatic Index (GSI) and gonad histological structure of male and female uceng fish (Nemacheilus fasciatus) on the period of immature and mature. Fish were dissected , weighed and the gonads were measured for gonadosomatic index. Moreover, gonad were fixed in NBF 5% for histological preparation by paraffin method and which than were stained by Hematoxylin-Eosin. The result were analyzed for both qualitatively and quantitatively. Qualitative analysis was conducted for gonad histological structure of male and female fish, while quantitative analysis was for Gonadosomatic Index (GSI) of fish respectively. The result showed that there were differences on the gonadosomatic index of males and females fish, the histological structure of ovarian follicle and the composition of the spermatogenic cells of immature gonads and mature gonads of the fish respectively. It was conclude that there are differences on Gonadosomatic Index and gonad histological structure on immature and mature of male and female of Uceng fish (N. fasciatus).
Histological Study of Phyllidia coelestis (Nudibranch) Epidermal Tissue from Pasir Putih, Situbondo Permadani, Karunia Galih; Retnoaji, Bambang
Indonesian Journal of Biology Education Vol 1, No 1 (2018): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Nudibranch are a mollusk which can be found in all zones of ocean. Further, they are distributed widely from subtropical to tropical areas. The Nudibranch is characterized by lack of a shell and usually have a bright shiny color. Nudibranch has a potency to be used in the medical field because they have chemical compound inside their body. These chemical compound are the result of secondary metabolites from symbiosis with zooxanthellae in nudibranch tissues.  This research aims to study the structure of epidermal tissue of the Phyllidia coelestis collected from Pasir Putih, Situbondo, East Java Indonesia. The results showed that the epithelial structure of the Phyllidia coelestis composed of cuboidal epithelium with vacuoles spread regularly along the mantle layer below the epidermal layer. Gland cells are rarely found in the outer epithelial layer. The foot epidermal layer consisted of simple ciliated columnar epithelial cells, with goblet-shaped mucous secreting glands among the epithelium. Gill tissue structure was composed of cell layers of cuboidal ciliated epithelial cells resting on a basement membrane and connective tissues
PERKEMBANGAN OVARIUM IKAN WADER PARI (Rasbora lateristriata Bleeker, 1854): PENDEKATAN HISTOLOGI Zulfadhli, Zulfadhli; Wijayanti, Nastiti; Retnoaji, Bambang
JURNAL PERIKANAN TROPIS Vol 3, No 1 (2016)
Publisher : Universitas Teuku Umar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.612 KB) | DOI: 10.35308/jpt.v3i1.34

Abstract

This study was to determinte of development ovarian wader pari fish (Rasbora lateristriata) as part of the process of reproduction, which is the basic for information to R. lateristriata management. This study was done in october 2014 - april 2015 at Histology and Embryology Animal Laboratory of Gadjah Mada University. The object of the research was larva R. lateristriata with average weight of 0.02 grams. They were kept during 3 months. The result data of histology were analized and observed discussed descriptively. The results of observation showed that ovarian R. lateristriata indicated asincronous development patterns. The development of ovarian in the 1st month was at the chromatin nucleolar phase, while perinucleolar phase appeared in the 2nd and 3rd month.
THE EFFECT OF ETHANOLIC EXTRACT OF CASHEW FRUIT PEEL ON THE LIVER HISTOLOGICAL STRUCTURE IN RAT (RATTUS NORVEGICUS BERKENHOUT, 1769) Zusrina, Laili Mufli; Retnoaji, Bambang; Wijayanti, Nastiti
Journal of Tropical Biodiversity and Biotechnology Vol 4, No 3 (2019): Desember
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.39275

Abstract

Cashew fruit peel is a waste produced from the cashew nut industry, and it has not been utilized optimally yet. Cashew peel extract has the potential to be used as a contraceptive agent, which capable of reducing reproductive capacity. However, its side effects on other tissue and organ such as liver not clearly studied yet. This study aims to determine the effect of ethanolic extracts of cashew peel on the histological structure of the white rat liver. In this study, 21 female white rats were used and be grouped for control (6 mice) which were treated with CMCMa 0.5% and 15 mice were treated with peel extract of 500 mg/kg body every day for one month. Liver for examination was collected sequentially at 3rd, 5th, 8th, 11th, and 14th of the estrous cycle. The liver was processed for histological observation and stained with Hematoxylin Eosin and Mallory Acid Fuchsin staining solution. The liver hepatocyte was observed for it abnormality and be scored to calculate the number of cell damage or abnormality. The result showed that peel extract-treated mouse liver was similar to control ones; we did not witness any evidence of fibrosis, pyknosis and cellular necrosis on either control or treated mouse. Statistical analysis by SPSS showed that the p-value between the control and treatment groups was 0.078 (> 0.05) so there was no significant difference between control and treatment. It could be concluded that ethanolic extracts of cashew nuts peel with a concentration of 500 mg/kg body weight caused no effect on the mouse liver histological structure. application with reduced-dosages of NPK fertilizers were arranged in a random block design with three replicates. The results show that large quantities of silica bodies attached to the surface of EFB fibers and amounting to 0.44% soluble Si. The FFB data indicated that the application of 75% NPK + 500 kg composted EFB + 2 L BioSilAc/ha/year on a five-year-old plant resulted in higher yield than that obtained from 100% standard dosage of NPK. The study also revealed that the application of EFB compost reduced 50% of BioSilAc dosage.