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PEMBENTUKAN GAHARU GYRINOPS VERSTEEGII OLEH BIOINDUKSI FUSARIUM SOLANI DENGAN TEKNIK SIMPORI Wahyuni, Resti; Prihantini, Amalia Indah; Anggadhania, Lutfi
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 1 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (666.085 KB) | DOI: 10.18343/jipi.25.1.152

Abstract

Agarwood is a non-timber forest product having high economic value. However, its population in nature is getting decrease. An effort to reduce the agarwood hunting in the nature is agarwood cultivation. One of agarwood inoculation methods is simpori as a modification of inoculation method using Fusarium solani and porous nails. The present study aimed to determine the effect of F. solani dosage to the quantity and quality of Gyrinops versteegii agarwood using simpori. The simpori technique used completely randomized design with 3 treatments and 10 replications. The treatments were F. solani at a dosage of 3 mL/porous nail (P0), F. solani at a dosage of 9 mL/porous nail (P1), and F. solani at a dosage of 6 mL/porous nail (P2).  The result showed that both dimensions of agarwood formation and the agarwood produced at 7 months after the first inoculation in all treatments were not significant. The highest length and the highest depth of agarwood formation were shown by P1 (5.1 cm) and P0 (7 cm) treatments, respectively. The highest agarwood production was observed in P1 and P2 treatments (0.032%). The visual quality of agarwood based on SNI 7631:2011 equal to kemedangan TG.C. The agarwood quality based on sesquiterpenes and chromone derivatives contents was different in all treatments. P2 treatment showed the highest content of sesquiterpenes and chromone derivatives (17.5%). The difference of F. solani dosages produce agarwood with the same quantity but different in quality when harvest 7 month after the first inoculation. Keywords: agarwood, phenylethyl chromone derivatives, sesquiterpenes, simpori
IDENTIFIKASI TIGA ISOLAT CENDAWAN PENGHASIL GAHARU DARI NUSA TENGGARA BARAT DENGAN MENGGUNAKAN PRIMER ITS DAN TEF 1-α Nugraheni, Y.M.M Anita; Anggadhania, Lutfi; Putranto, Riza Arief
Jurnal Pemuliaan Tanaman Hutan Vol 9, No 2 (2015): Jurnal pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2015.9.2.77-90

Abstract

Several isolates of agarwood-forming fungus in Gyrinops versteegii have been isolated from the result of exploration in Lombok and Sumbawa Islands. This study aimed to identify the three fungus associated with the agarwood formation in G. versteegii originated from Lombok Tengah, Alas, and Lombok Barat, Nusa Tenggara Barat. The three fungus cultured in liquid medium PDB (Potato Dextrose Broth) and incubated for 1 month in shaken culture. The mycelium of each fungus was harvested for DNA isolation. Amplification is done by Polymerase Chain Reaction (PCR) using the Primers ITS and TEF with obtained amplicon having base length ranging of 300-600 bs. BLAST analysis showed that the three fungus have similarity with Fusarium solani. These results were confirmed by phylogenetic tree where all fungus has genetic relationship with F. solani.
EFEK LAJU KARBONDIOKSIDA (CO₂) TERHADAP MORFOLOGI DAN LAJU PERTUMBUHAN POPULASI Spirulina platensis (Gomont) [The Effect Of Carbon Dioxide (Co₂) Rate To The Morphology And The Growth Rate Of Spirulina Platensis (Gomont) Population] Anggadhania, lutfi; Nugroho, Andhika Puspito
Journal Penelitian Kehutanan FALOAK Vol 1, No 2 (2017): Jurnal Penelitian Kehutanan FALOAK
Publisher : Balai Penelitian Dan Pengembangan Teknologi Hasil Hutan Bukan Kayu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpkf.2017.1.2.75-84

Abstract

ABSTRACTCarbon dioxide pollution occurs due to increase the use of fossil fuels for industry and transportation. Due to deforestry, the absorption of carbon dioxide by terrestrial environment is reduced. This will lead the increased of carbon dioxide absorption by the sea. The absorption of carbon dioxide by the ocean will lead the changes in ocean chemistry and affects the marine ecosystems. Spirulina platensis, as a cosmopolitan organism that can use inorganic carbon that absorbed by the ocean become one of microorganism that has effect by these changes. This research aims are to study the effect of carbon dioxide rate to the morphology and the growth rate of Spirulina platensis population. This research method is completely randomized design with three treatments and three replications. The period of treatment started in the exponential phase with carbon dioxide rate at 0.1 lpm, 0.2 lpm, and 0.4 lpm. The results showed that carbon dioxide is given can be used by S. platensis to stimulating the growth but this will also shorten the growth kinetics. This is also reflected in the results of the statistical analysis that there is no significant defference (p>0,05). And the morphological responses of S. platensis are fragmentation and lysis cell.ABSTRAK Pencemaran karbondioksida terjadi karena peningkatan penggunaan bahan bakar fosil untuk industri dan transportasi. Akibat terjadinya deforestri penyerapan karbondioksida oleh lingkungan terrestrial berkurang, sehingga terjadi peningkatan penyerapan karbondioksida oleh laut. Penyerapan karbondioksida oleh laut akan menyebabkan perubahan sifat kimia laut yang berdampak pada ekosistem laut. Spirulina platensis sebagai organisme kosmopolitan yang terdapat di laut dapat menggunakan karbon anorganik yang terserap dalam laut. Penelitian ini bertujuan untuk mempelajari efek laju karbondioksida terhadap morfologi dan laju pertumbuhan populasi Spirulina platensis. Metode penelitian ini adalah rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan. Masa perlakuan dimulai pada fase eksponensial dengan laju karbondioksida 0,1 lpm, 0,2 lpm, dan 0,4 lpm. Hasil penelitian menunjukkan bahwa karbondioksida yang diberikan mampu digunakan oleh S. platensis untuk meningkatkan pertumbuhan tetapi juga akan memperpendek kinetika pertumbuhan. Hal ini juga tercermin pada hasil analisis statistiknya yang tidak ada beda nyata (p>0,005). Secara morfologi respon S. platensis terhadap pemberian karbondioksida adalah terjadinya fragmentasi dan lisis sel.
UJICOBA PERBANYAKAN VEGETATIF SAMBUNG TANAMAN Gyrinops versteegii Setyayudi, Ali; Nugraheni, Y. M. M. Anita; Anggadhania, Lutfi; Mansyur, Mansyur
ULIN: Jurnal Hutan Tropis Vol 1, No 2 (2017)
Publisher : Fakultas Kehutanan Universitas Mulawarman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32522/u-jht.v1i2.799

Abstract

?Dalam pembudidayaan tanaman penghasil gaharu masyarakat banyak memanfaatkan anakan alam dan biji, namun sumber benih yang ada belum teruji serta belum dibangun atas dasar kemampuan menghasilkan gaharu sehingga produktivitas gaharu yang dihasilkan belum konsisten. Peningkatan produktivitas gaharu dapat didekati melalui peningkatan kemampuan tanaman merespon serangan dan membentuk gaharu, namun kemampuan setiap tanaman berbeda-beda meskipun dalam jenis yang sama. Oleh karena itu diperlukan teknik kloning agar diperoleh tanaman berkemampuan sama. Kloning tanaman dapat dilakukan melalui perbanyakan vegetatif, salah satu diantaranya adalah dengan teknik sambung. Penelitian ini ditujukan untuk mengetahui keberhasilan teknik perbanyakan sambung pada tanaman penghasil gaharu jenis Gyrinops versteegii. Penelitian dilakukan secara eksperimental dengan tiga perlakuan teknik sambung yaitu sambung atas, samping, dan menyusu. Ulangan berupa pohon asal scion sebanyak lima ulangan dengan setiap ulangan dibuat 4 unit sambungan sehingga total terdapat 60 unit percobaan. Hasil yang diperoleh adalah rata-rata keberhasilan teknik sambungan sebesar 36,67% dengan sambungan menyusu memiliki keberhasilan tertinggi sebesar 65%.
PELATIHAN TEKNOLOGI BIO-INDUKSI UNTUK PETANI GAHARU DI DESA PEJARING, KABUPATEN LOMBOK TIMUR Wangiyana, I Gde Adi Suryawan; Wanitaningsih, Sad Kurniati; Anggadhania, Lutfi
Agrokreatif: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 6 No. 1 (2020): Agrokreatif Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrokreatif.6.1.36-44

Abstract

Agar wood farmer of Pejaring Village have used traditional method to induce resin formation on agarwood tree. They used mechanical method induction by nail and machete tools as well as chemical method induction by chemical liquid that the called by ?infus?. These traditional methods influenced resin formation on agarwood tree at their agarwood plantation. That resin production was low in productivity and low in efficiency. The purpose of this community service was to conducted workshop of bio-induction technology for Pejaring Village agarwood farmer to solve their problem. Agarwood farmer of Pejaring Village were directly involved in all bio-induction training activity. This training was started by socialization of program through FGD with Agarwood Farmer of Pejaring Villange. Practical stage was started by selecting agarwood tree for sample by themselves. After selecting process has been done, training continued by bio-induction of agarwood sample using bore and injecting method also by themselves. The last stage was monitoring and evaluating of resin production on agarwood sample as result of bio-induction. In this stage, they got opportunity to give self-assessment of those resin production by organoleptic test. Bio-induction technology could stimulate resin formation on agarwood tree sample at 3 months after induction. This result was much better comparing to the result of traditional induction method that they used to conducted. Organoleptic test has confirmed that resin has quite good fragrance quality. It could be concluded that Pejaring Village agarwood farmers have understood about bio-induction technique and could applied that technology to replace traditional technology.
PENGOLAHAN SAMPAH BUAH DAN SAYUR DARI PASAR INDUK MANDALIKA KOTA MATARAM NUSA TENGGARA BARAT SEBAGAI MEDIA TUMBUH FUSARIUM SP INOKULAN GAHARU Wangiyana, I Gde Adi Suryawan; Sanjaya, Arbi; Anggadhania, Lutfi
IJEEM - Indonesian Journal of Environmental Education and Management Vol 5 No 2 (2020): IJEEM: Indonesian Journal of Environmental Education and Management, Volume 5 Nom
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/IJEEM.052.06

Abstract

The purpose of this research is to process fruit waste and vegetable waste from Mandalika Market at Mataram City as growth medium for Fusarium Sp agarwood inoculant. Collected fruit waste and vegetable waste were processed by grinding and filtrating into filtrate part and residue part. Filtrate part and residue part were formulated as combination growth media including: Fruit waste filtrate, vegetable waste filtrate, mix fruit-vegetable waste filtrate, fruit waste residue, vegetable waste residue and mix fruit-vegetable residue. Fusarium Sp. were sub-cultured into PDA before cultured on combination growth media. Colony diameter of Fusarium isolates were measured every day for seven days incubation period. Fusarium Sp. growth on media from fruit waste was better than its growth on media from vegetable waste. Fusarium Sp. has higher colony diameter on residue growth media compare to its colony diameter on filtrate growth media. On the other hand, filtrate growth media could support Fusarium Sp. to produce several pigmentations. This pigmentation could be an important key character for further taxonomical study. It could be concluded that fruit waste and vegetable waste from residue part could support maximum diameter colony of Fusarium Sp. while filtrate part of this waste could support Fusarium Sp. to produce pigmentation for taxonomical purpose.