Sulistyawati, Purnamila
Center for Forest Biotechnology and Tree Improvement (CFBTI)

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DETEKSI KEBERADAAN Phytophthora spp. DI AIR Sulistyawati, Purnamila
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 3 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.3.199-212

Abstract

The genus of Phytophthora is a destructive plants pathogen. However the existence of these species in plant tissue was hardly to detect because the fungus may also be present as resistant propagules in soil or spread through waterways. This study aimed to test the FTA card as a direct bait to obtain the DNA of  Phytophthora spp and also to investigate the suitability of FTA card as a sampling method. This research used several Phytophthora baits including FTA card followed by DNA extraction and PCR used ITS primers and genus/species specific primers. The result of the study showed that FTA card can be used as a direct bait for Phytophthora spp. and to sample fungal DNA from other Phytophthora baits. However, further optimisation and validation are still required.
KERAGAMAN GENETIK ANAKAN Shorea leprosula BERDASARKAN PENANDA MIKROSATELIT Sulistyawati, Purnamila; Widyatmoko, AYPBC; Nurtjahjaningsih, ILG
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 3 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.3.171-183

Abstract

Genetic diversity value of offspring might indicate a reproductive success in a forest. Aim in this study was to access genetic diversity values of offspring of Shorea leprosula from different forest types. Leaf samples were collected from six population i.e. a plantation from Carita, and five natural forests from Gunung Bunga A and B, SBK, ITCI and Gunung Lumut. Using four microsatellite markers, the results showed that SBK population maintained high value of genetic diversity. Values of expected heterozygosity (HE) ranged between 0.717 (Carita) and 0.836 (SBK). Values of coefficient inbreeding (F) were insignificant deviated from Hardy-Weinberg Equilibrium, but the value was significant in SBK population. PCA analysis demonstrated a cluster among Gunung Bunga A, SBK, ITCI, and Gunung Lumut. Amova showed that different province significantly contributed 1% to the value of genetic diversity of S. leprosula.
KARAKTERISTIK PEMBUNGAAN DAN SISTEM PERKAWINAN NYAMPLUNG (Calophyllum inophyllum) PADA HUTAN TANAMAN DI WATUSIPAT, GUNUNG KIDUL Nurtjahjaningsih, ILG; Sulistyawati, Purnamila; Widyatmoko, AYPBC; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 2 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.2.65-78

Abstract

Flowering are influenced by internal factors, such as genetic and phytohormone, and environment factors, such as sunlight and nutrition intake. The flowering characteristics influence fruiting and genetic diversity seedlings through mating systems. This study aims to assess flowering and fruiting characteristics and to determine pattern of mating system of a Calophyllum inophyllum plantation at Watusipat, Gunung Kidul. Flowering and fruiting were observed at 4 locations, 3 parts of crown, and 4 main directions to know the effects of sunlight, nutrition intake and phytohormone in the flowering process. Mating system was assessed by comparing genetic diversity values between parent trees and offsprings. The values of genetic diversity were analyzed using 5 RAPD primers with 17 polymorphic loci. Analysis of variant showed that the locations, crown parts, directions and interaction between a location and direction significantly affected to differences number of flowers and fruits. Values of genetic diversity (h) of parent trees ranged between 0.1471 and 0.3056. The values increased at almost overall offsprings; it ranged between 0.2864 and 0.3750. Values of genetic distance (Da) between parent trees were high and very high (0.197 – 0.364), but the values was decreased between parent trees and their offspring, even between offspring populations. A dendrogram showed two main clusters; first cluster consisted parent trees at up edge with rare trees and second cluster consisted sub cluster parent trees at up edge; sub cluster parent trees at down middle; and sub cluster parent trees at down edge and overall offsprings. Flowering/ fruiting characteristics and pattern of mating systems of C. inophyllum were briefly discussed. Key words : Flowering characteristic, genetic diversity, Calophyllum inophyllum, RAPD analysis
SCREENING PENANDA MIKROSATELIT Shorea curtisii TERHADAP JENIS-JENIS SHOREA PENGHASIL TENGKAWANG Nurtjahjaningsih, ILG; Widyatmoko, AYPBC; Sulistyawati, Purnamila; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.49-56

Abstract

Screening primer is an effective method to develop microsatellite markers from related taxa. Aim of this study was to develop microsatellite markers of four Shorea producing tengkawang oil, i.e. Shorea gysbertiana, Shorea macrophylla, Shorea pinanga and Shorea stenoptera by screening microsatellite primers of Shorea curtisii. Leaf samples of the four Shorea were collected from nursery at Center for Forest Biotechnology and Tree Improvement Research in Yogyakarta. Four microsatellite primers of S. curtisii i.e. Shc-1, Shc-2, Shc-7 and Shc-9 had been used to screen. Results showed that numerous alleles were shared among the Shorea. The expected heterozygosity (HE) for locus Shc-1 ranged between 0.594 and 0.722; locus Shc-2 ranged between 0.219 and 0.611; locus Shc-7 ranged between 0.594 and 0.778; and locus Shc-9 ranged between 0.594 and 0.844. Coefficient of inbreeding (FIS) value was low and it was insignificant deviated from Hardy-Weinberg Equilibrium (HWE) at  almost all loci except Shc-1 of S.  pinanga. A dendrogram showed two clusters; S. gysbertsiana and S. macrophylla represented in one cluster, while S. pinanga and S. stenoptera represented in another cluster. Therefore the developed microsatellite markers are possible to be applied for studying population genetics and mating system of these species.
KEKERABATAN GENETIK ANAKAN ALAM ULIN (Eusideroxylon zwageri TEIJSM. & BINN.) MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA Nurtjahjaningsih, ILG; Sukartiningsih, Sukartiningsih; Amarta Saranti, Anna Puspa; Sulistyawati, Purnamila; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (411.592 KB) | DOI: 10.20886/jpth.2017.11.1.25-32

Abstract

The study aimed to assess genetic diversity and genetic relationship of ulin wildlings randomly collected from a nursery and originated from Bukit Soeharto natural forest, East Kalimantan. DNA templates were extracted from leaf samples of 1.5 years old wildings. Five RAPD primers consisted 55 polymorphic loci were used for genetic studies. Genetic diversity and relationship were analyzed using GenAlex software. The results showed moderate mean value of genetic diversity (HE=0,345, SE 0,015) of the wildings. Forty eight wildings were clustered in only 3 groups; almost all wildings (65%) were clustered in one main cluster. Moreover, 4 wildlings were clones (8%). In conclusion, the 48 wildings of ulin consisted high genetic relationship and individual clones that reflects the low genetic diversity of this species.
THE USE OF FTA CARD ON DNA SAMPLE PREPARATION FOR MOLECULAR OF PLANT DISEASE IDENTIFICATION Sulistyawati, Purnamila; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 1, No 3 (2007): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2007.1.3.123-130

Abstract

Accurate and guick identification of pathogen is key to control the spread of plant disesases. Morphological identification is often ineffective because  it requires fruit body which often are not presence, rely on characters which may be highly variable within and among species and can be slow and time consuming. Molecular identification of plant disease can overcome most of the shortcomings of morphological identification. Application of FTA Cardn for sample collection is crucial for the success of molecular identification of phatogen. The study present an earlier results on the suitability of FTA Card to collect sample in situ. The study was done on 4 different sample namely pure culture of fusarium sp., infected leaf, soil and seeds which assumed to have mycelium. The result indicate that FTA Card is effective as a collecting media of pathogen for most samples, paticularly for mycelium culture. Furthur experiments are still required to ensure the suitability of the card for leaf, seeds and soil samples.
PENGUJIAN KARTU FTA SEBAGAI ALAT SAMPLING DNA JAMUR PATOGEN DARIBERBAGI BAGIAN TANAMAN YANG TERINFEKSI Sulistyawati, Purnamila
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 2 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.2.93-104

Abstract

FTA  card offers a simple and fast method for retrieval of DNA samples at room temperature and storage of DNA in the short and long term. This will fasiliate the detection and identification of plant pathogens rapidly; increasing the number of samples can be collected, stored and transported in the field, especially from remote locations. The purpose of this study is to investigate  the suitability of FTA cards as a new method for sampling DNA from multiple infected of  plant tissues such as leaves, bark and roots. The results of this study shows that the FTA card suitable for use as one method of smpling because its quick and accurate. Series of PCR using ITSIF/ITS4 and ITS3/ITS4 primer pairs were done to detect the fungal DNA from the FTA card Nested PCR has been done using species – specific primers to identify the fungus without cloning. Optimization of methods to maximize the use of FTA cards as a method of sampling and DNA extraction which is simple, fast, accurate and safe for the environment is still needed. 
STRUKTUR GENETIK Calliandra calothyrsus DI INDONESIA MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD) Nurtjahjaningsih, ILG; Sulistyawati, Purnamila; Rimbawanto, Anto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2016.10.1.31-38

Abstract

Calliandra calothyrsus was introduced to Indonesia from Guatemala in 1930s and had been planted widely throughout Indonesia for fuel wood. Genetic diversity within and among population is an important factor for initiating breeding program. Aim in this study was to evaluate genetic structure of C. calothyrsus in Indonesia, to obtain a better understanding of the history of the distribution and efficiency of breeding strategy of this species. Leaf samples were collected in a seedling seed orchard plot from 10 populations. Six RAPD markers consisted of 34 loci were used to analyze genetic diversity and genetic structure. The results showed that genetic diversity was in low to moderate level (mean HE= 0.321). The AMOVA analysis showed that genetic differentiation among geographical sources was significant, also among populations within geographical sources and among individual within populations as well. It was revealed that the 10 populations originated from only two ancestors. The limited number of ancestors resulted low to moderate genetic distances among populations (mean Da=0.070). In conclusion, C. calothyrsus has moderate genetic diversity within population and weak genetic structure among populations. Genetic diversity in population/individual level should be considered as a selection unit in the breeding strategies.
KERAGAMAN GENETIK POPULASI KAYU MERAH (Pterocarpus indicus Willd) MENGGUNAKAN PENANDA RAMDOM AMPLIFIED POLYMORPHISM DNA Sulistyawati, Purnamila; Widyatmoko, AYPBC
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.383 KB) | DOI: 10.20886/jpth.2017.11.1.67-76

Abstract

Kayu merah ( Pterocarpus indicus Willd) which listed as one of the IUCN Red List of Threatened Species in the category Vulnerable (VU A1D) is naturally distributed throughout Indonesia included Java, Sulawesi, Maluku, Bali, East Nusa Tenggara, West Nusa Tenggara and Papua. Random Amplified Polymorphic DNA (RAPD) molecular markers have been used to analyze the genetic diversity of six (6) natural populations of kayu merah from Seram Island, Manggarai Timur-Flores, Ngada-Flores, Soe-Timor Tengah Selatan, Kefamenanu-Timor Tengah Utara and Kupang. Ninety-nine (99) polymorphic loci were obtained from twelve RAPD primers. The mean of genetic diversity was 0.2024. Population with the highest genetic diversity was Soe (0.2925) and the lowest genetic diversity was Ngada (0.1212). The highest genetic distance among the populations was between Ngada and Kefamenanu (0.376), while the nearest genetic distance was between Ngada and Manggarai Timur (0.060). The informations resulted from this study are important to support the tree improvement and conservation programme of kayu merah.