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KARAKTERISASI ENZIM PHYTASE MIKROBA UNTUK MENINGKATKAN KUALITAS BAHAN PAKAN UNGGAS Purnamasari, Listya; Miswar, Miswar
Journal of Livestock Science and Production Vol 2, No 2 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

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Abstract

Asam phytat merupakan salah satu faktor antinutrisi yang terdapat dalam pakan ternak yang berasal dari serealia yang dapat menurunkan kecernaan nutrien bahan pakan sehingga mengganggu produktivitas ternak utamanya ternak monogastrik. Penurunan produktivitas disebabkan karena keterbatasan enzim yang mampu menguraikan asam phytat. Tujuan dari penelitian ini adalah mengetahui aktivitas enzim phytase yang berasal dari mikroba yang ditumbuhkan pada media padat. Media padat yang digunakan ada 4 macam yaitu jagung, dedak padi, dedak jagung dan beras merah. Mikroba yang digunakan yaitu bakteri Basillus subtilis. Pengamatan dilakukan pada rentang pH 4-7 dengan masa inkubasi selama 5 hari pada suhu ruang 25OC di atas shaker. Hasil penelitian menunjukkan bahwa aktivitas fitase dari Basillus subtilis memiliki rentang pH yang panjang yaitu 4-7. Aktivitas dari media padat yang digunakan tidak menunjukkan perbedaan yang nyata sehingga semua media dapat digunakan sebagai media pertumbuhan bakteri yang memproduksi enzim phytase. Enzim fitase yang dihasilkan pada media jagung yaitu 0,313 µg/ml media dedak padi yaitu 0,317 µg/ml media dedak jagung yaitu 0,313 µg/ml dan media beras merah yaitu.0,315 µg /ml. Kesimpulan dari penelitian ini yaitu bakteri Basillus subtilis dapat digunakan untuk memproduksi enzim phytase dan mampu menurunkan kandungan asam fitat pada bahan pakan.Kata kunci : Asam fitat, biji-bijian, enzim phytase, fermentasi media padat
RESPON FISIOLOGIS DAN PALATABILITAS DOMBA EKOR TIPIS TERHADAP LIMBAH TAUGE DAN KANGKUNG KERING SEBAGAI PAKAN PENGGANTI RUMPUT Purnamasari, Listya; Rahayu, Sri; Baihaqi, Muhammad
Journal of Livestock Science and Production Vol 2, No 1 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

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Abstract

Physiological response are influenced by environmental conditions, such as climate change and the feed. Limitied of land crop and farm cause difficulties to find grass as fiber feed. This research aimed to study the effect of dried kale and bean sprouts waste for substitution the grass on the physiological response of male javanese thin thailed sheep (under one years old) and their palatability. Feed treatment were : P1 (50% concentrate+50% grass), P2 (50% concentrate+50% dried kale), and P3 (50% concentrate+50% bean sprouts waste) respectively. The parameters were palatability, rectal temperature, heartbeat and respiration frequency. Analyze method used completely randomized design with three treatments of feeding and 4 replications was used. Data processed by ANOVA and Tukey’s for further test. The experimental result showed that feed treatment were not significant (P>0.05) to rectal temperature, heartbeat and respiration frequency. The best palatability seen on P2 feed (50% consentrate + 50% mung bean sprout waste). The conclusion were bean sprout waste and dried kale can be used to substitute grass, they had a good palatability and they did not give bad effect to physiological of javanese thin tailed sheep.The conclusion of this study, bean sprout waste and dried kale has a good palatability and were not significant to physiological response of javanese thin tailed sheep so it can be used to substitute grass.Keywords: dried kale, physiological responses, javanese thin tailed sheep, bean sprout waste, palatability
TEKNOLOGI PEMANFAATAN LIMBAH PADAT PENGOLAHAN KERIPIK SINGKONG MENJADI PAKAN PELET AYAM PEDAGING DI DESA BARATAN KABUPATEN JEMBER Purnamasari, Listya; Pratiwi, Nurul; Siswoyo, Tri Agus
Journal of Livestock Science and Production Vol 2, No 1 (2018): Journal of Livestock Science and Production
Publisher : Universitas Tidar

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Abstract

      Baratan Village, Patrang Sub-district, Jember District has a high potential production of cassava chips by Home Industry (IRT). Solid waste of cassava (cassava husk) from cassava processing into cassava chips has not been utilized properly. Cassava husk can be used as raw material for animal feed. The purpose of this program is the empowerment of the community of Baratan IRT in the processing of cassava husk into broiler feed and to increase the value of the waste and can improve the economic of the IRT in Baratan Village. Cassava husk has a deficiency because of the antinutrient content (Cyanide Acid) and dangerous when consumed. The methods used in this program are counseling and chipping of cassava with chipping tools, counseling and training of utilitation of cassava waste, counseling and training of broiler feed formulation, pelet making, mentoring, monitoring and evaluation. The result of this program is the IRT Baratan Village Jember Regency is able to process the solid waste from processing of cassava chips into broiler feed (pelet). Guidebooks can be used by IRT well so they can treat the waste into feed independently. IRT cassava chips as a partner of this program can implement and apply the technology that has been given and strive to always continuous in the manufacture of feed and pellet product development in an effort to improve the welfare and sustainability of the program.Keywords: cassava waste, pelet feed, Baratan Village
Physiological Response on Broiler Chicken’s Liver Supplemented Amino Acid Metionine-Cystine in Feed Contaminated with Aflatoxin B1 Purnamasari, Listya; Agus, Ali; Noviandi, Cuk Tri
Journal of Livestock Science and Production Vol 3, No 1 (2019): Journal of Livestock Science and Production
Publisher : Universitas Tidar

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Abstract

               The content of AFB1 in feed with low levels and spend a long time, will cause primary damage or primary liver carcinoma. This study aims to reduce the toxicity of AFB1 with amino acid methionine-cystine supplementation in broiler chicken feed. This study used a 3 × 3 factorial design with methionine-cystine amino acid levels (M + C: 75, 100 and 125%) and AFB1 levels (0, 200, and 400 ppb). The variables collected were liver physiology, liver histopathology, SGPT levels, and SGOT levels. Observations of liver physiology showed that feed containing aflatoxin without methionine-cystine amino acid had a paler yellowish color (T4, T7 and T8). Pathological examination resulted that aflatoxicosis will attack the liver. Transition amino acid cystines in chicken feed contaminated with AFB1 did not occur in blood SGPT levels. Blood SGOT levels were highest at 21 days of age, namely T2 (M + C 100%) and T3 (M + C 125%) at AFB1 0 ppb which showed excess liver damage. The administration of methionine-cystine amino acids of 75, 100 and 125% in chicken feed contaminated with AFB1 0, 200 and 400 ppb consumed by broilers carries a risk of physiological and pathological damage to chicken liver.