Kurnianingsih, Nia
Departement of Physiology, Faculty of Medicine, Brawijaya University

Published : 2 Documents
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MONOSODIUM GLUTAMATE EXPOSURE AT EARLY DEVELOPMENTAL STAGE INCREASES APOPTOSIS AND STEREOTYPIC BEHAVIOR RISKS ON ZEBRAFISH (DANIO RERIO) LARVAE Kurnianingsih, Nia; Utami, Juliyatin Putri; Nurdiana, Nurdiana; Lyrawati, Diana
INDONESIAN JOURNAL OF PHARMACY Vol 27 No 3, 2016
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1107.374 KB) | DOI: 10.14499/indonesianjpharm27iss3pp128

Abstract

Excessive glutamate may give neurotoxic effects and contribute to Autism spectrum disorder(ASD). In this study, we investigated prolonged exposure effects of 10 µg/mL Monosodium Glutamate (MSG) on intracellular calcium level, bax, bcl-2, ratio of bax/bcl-2 genes expression, caspase-3, apoptosis of brain cells and stereotypic behavior of Zebrafish (Danio rerio) larvae at early developmental stages. Genes expression were determined by real time PCR, caspase-3 using ELISA, intracellular Ca2+ and apoptotic cells of brain using confocal microscopy, locomotor activity by using crossing lines assay whereas stereotypic behavior by circle swimming. The results indicated that MSG exposure increased brain bax and bcl-2; and caspase-3; intracellular Ca2+; and apoptosis; stereotypic behavior; and decreased locomotor activity. Termination of MSG treatments resulted in recovery of bax, bcl-2, caspase-3 basal levels and stereotypic behavior. In conclusion, MSG exposure at early embryonic stage increased brain cell damage and risk of behavior changes.
Association Between Time Spent for Internet Gaming, Grade Point Average and Internet Gaming Disorder Risk Among Medical Students Kurnianingsih, Nia; Ratnawati, Retty; Yudhantara, Dearisa Surya; Prawiro, Rizqi Bagus Setyo; Permatasari, Maryam; Rachma, Heidyana; Ariadi, Adilla Surya
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.701 KB) | DOI: 10.21776/ub.rjls.2018.005.03.1

Abstract

The negative and positive consequences of internet games were less explored among medical students’ population. The aim of this current study was to investigated the association between time spent for playing internet games and Grade Point Average (GPA) as well as Internet Gaming Disorder (IGD) risk among medical students. A total of 438 students in academic year 2016-2017 completed the self-report questionnaire, that covered socio-demographic information, GPA, time spent for internet gaming/day, and IGD screening. The statistical analysis used c2 test. The average age of the respondents was 20.2 ±1.57 year. The respondents comprised of 37.13% male and 62.87% female students. The percentage of male and female players was 97.5% and 93.8% respectively. Male students were more at risk of IGD (29.56%) compared to their female counterparts (10.04%). Although time spent for internet gaming/day was associated with IGD risk (p<0.001), it was not associated with GPA (p=0.501). There was no significant GPA difference between students who played internet games <3 hours/day and >3 hours/day and those who never play at all. Further study is required to explore the factors related to GPA among medical students.