Tamad, Tamad
Fakultas Biologi | Universitas Jenderal Soedirman

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Journal : Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal

Seleksi Isolat Bakteri Pelarut Fosfat (BPF) untuk Mengembangkan Inokulum Efektif Tamad, Tamad; Radjagukguk, Bostang; Hanudin, Eko; Widada, Jaka
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.265

Abstract

The phosphate solubilizing bacteria (PSB) can release insoluble phosphate in soil by anorganik-P dissolution, organic-P mineralization, and blocking of soil adsorption site.  The selection of effective PSB, therefore,  is urgently required to study the effectiveness of PSB.  The research was arranged to determine: 1) P-solubi-lization or P-mineralization, 2)  physiology, and 3) PSB inoculant improvement.  The research was arranged in complete random design, with PSB isolate as treatment.  The variables  were: soluble-P, mineralize-P, adsorption-P, pH, PSB population, phosphatase and phythase activity. The  result showed that PSB isolate 1 = Pseudomonas trivialis, 5 = Pseudomonas putida, and 9 = Pseudomonas fluorescens, were the best in P solubilization or P mineralization.  Solubilization efficiency of isolate were 1 = 291%, 5 = 280%, and 9 = 347%.  Five days incubation (the end of log phase or early of stationary phase) was the best time to culture harvest for PSB inoculant formulate.  Within twelve months age of culture, population stability of PSB inoculant decreased between 81 and 88%,  and P solubilizing stability PSB inoculant decrease between 65 and 81%. Decreasing of P solubility to P source types of PSB inoculant was AlP > FeP > PR > CaP.
Karakter dan Potensi Inokulum Bakteri Fosfat dalam Melepaskan Fosfor Tamad, Tamad
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 2 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.2.212

Abstract

Soil bacteria group which can release soil P-adsorbed is phosphate bacteria (PB). PB can release soil P-adsorbed through: 1) acidification, 2) chelating 3) ligand exchange 4) mineralization and 5) compete colloid sorption site. PB have a potential to release soil P-adsorbed. This study was aimed to determining the character and PB inoculant potential in soil P-adsorbed release. The results of BLASTn PB showed that  isolate 1 was Pseudomonas trivialis, isolate 5 was Pseudomonas putida and  isolate 9 was Pseudomonas fluorescens. Based on the growth curve on day 5th (the end log. phase) population of Pseudomonas trivialis was 1010 CFU/mL, Pseudomonas putida was 1014 CFU/mL and Pseudomonas fluorescens was 1017 CFU/mL. Storage inoculant PB population decrease 97-84 % and 80-65 % PB lowering capabilities.
N-Acyl Homoserine Lactones sebagai Signal Quorum Sensing untuk Meningkatkan Efektifitas Bakteri Fosfat Tamad, Tamad
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 31, No 1 (2014)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2014.31.1.220

Abstract

Phosphate bacteria (PB) are able to release P-adsorption by soil. PB  effectiveness in releasing adsorption P  controled by quorum sensing (QS) signal. PB  produces a QS signal  as N-acyl homoserine lactones (N-HSL). The aim of this study are to determine the type of N-HSL as QS signal of PB (Pseudomonas trivialis, P. putida and P. fluorescens) and find the source of N-HSL from root extracts of some plants (rice, corn, bamboo, banana and peanuts). Analysis of N-HSL using HPLC (Hitachi UV-VIS detector L-2420), L-2200 autosampler (20 mL), L-2130 pump and column C OOF-4250-CO/10 µm LaChrom Ultra 18 (2 µm) 100 A 150 x 4.60 mm 10 m KPOW 490065-1 (Phenomenex), temperature 60° C, flow rate of 0.9 mL/minute  and a gradient of 30-100 % in 1.0 minutes. Standard N-HSL  is C4-HSL, 6, 8, 10, 12 homoserine lactones (Sigma-Aldrich, Germany) was dissolved in acetonitrile (Merch, India) with a concentration of 50 mM. P-dissolved by PB determined by staining NVM and a spectrophotometer at a wavelength of 413 nm. PB population is determined by the OD (optical density) at a wavelength of 600 nm. PB  populations  on medium Pikovskaya influenced by PB isolates, the type of P sources and duration of incubation. N-HSL generated by PB highest is Butanoyl (C4) homoserine lactones. PB isolates 9 and Ca-phosphate sources produce N-HSL most. Root extract of rice, corn, bamboo, bananas and peanuts can be a source of   N-HSL. Soluble phosphorus from medium Pikovskaya influenced by the type of PB isolates and source of P.