Krisantini, Krisantini
Journal of Tropical Crop Science

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Evaluation of Growth, Flowering and Seed Morphology of Batflower, Tacca chantrieri Andre Ajisyahputra, Nikko Rizky; Palupi, Endah Retno; Krisantini, Krisantini; Wiendi, Ni Made Armini; Mulyono, Joko
Journal of Tropical Crop Science Vol 4 No 2 (2017): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

Tacca chantrieri is a perennial plant belongs to Taccaceae family that has the potential to be marketed as an ornamental plant due to its unique shape and dark color inflorescence. To date, very limited publication on T. chantrieri are available. The aim of this research was to examine plant growth pattern and flower phenology of T. chantrieri from West Borneo, Indonesia, and from Queensland, Australia in relation to their potential development as ornamental pot plants. The study was extended to examine T. chantrieri seed morphology with an inclusion of an accession from Thailand.  The field experiment was conducted at the Leuwikopo experimental station in Darmaga, Bogor, Indonesia. Seed morphology examination was conducted at the Histology Laboratory of Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. T. chantrieri from West Borneo blooms six times a year whereas the Queensland accession twice a year. West Borneo accession had smaller bractea, fewer numbers of flowers per inflorescence and shorter flower stalk than Queensland accession. In addition, T. chantrieri Queensland grew faster than Borneo with the one leaf formed every two to three weeks, whereas it was at least four weeks in the Borneo accession. Both accessions were susceptible to leaf brown spot disease caused by Cercospora taccae. T. chantrieri Queensland seeds are oval dark brown, whereas West Borneo and Thailand accessions were shaped like kidneys (reniform) with a lighter brown color. This study demonstrated that there is great diversity in T. chantrieri including plant morphology, seed shape and color, which are important for species identification and for development of T. chantrieri as ornamental pot plant.
Characterization of 13 Accessions of Purslane (Portulaca sp.) from Bogor, West Java, Indonesia Setiawan, Farensa Ikman Dedi; Aisyah, Syarifah Iis; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 3 No 3 (2016): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

Characterization is part of crop the breeding process aims to protect biodiversity and preserve local germplasm. Indonesia has two known species of Portulaca, Portulaca oleracea and P. grandiflora. Thirteen Portulaca accessions have been collected from Bogor area, comprising five accessions of Portulaca oleracea and eight accessions of Portulaca grandiflora. This study was aimed at the characterization of the 13 accessions and was undertaken at Indoflower BLST, Bogor, between October 2015 to February 2016. The results showed a significant difference in the color of flowers between the two species of Portulaca. The most significant difference is the overall appearance of P. oleracea species. Character color and shape of the flowers between the P. grandiflora white (GW) and P. oleracea are similar.  Significant differences between the species exist in relation to the root systems, but the root systems of accessions within each species are generally similar.  The branching characteristics between the two species and among accessions are generally similar, with branches emerging through the base of leaves or nodes.   However, there are differences in the leaf characteristics between the two species. This study has provided information on flower characteristics of P. grandiflora and P. oleraceae which will be valuable for future breeding efforts in Portulaca. 
Proliferation of Protocorm-Like Bodies of Dendrobium mannii and Dendrobium mirbelianum in Chitosan-containing Media In Vitro Rahmah, Syifaur; Wiendi, Ni Made Armini; Suwarno, Willy B.; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 2 No 3 (2015): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

Dendrobium mannii and Dendrobium mirbelianum were rare and endangered orchid species. In vitro micro propagation could be used to conserve and proliferate these species. The aims of this study were to determine the effects of chitosan on proliferation of the protocorm-like-bodies (PLBs) of in vitro grown Dendrobium mannii and D. mirbelianum. Chitosan has been reported as an important component to promote orchid growth in vitro. In this study chitosan was included in the in-vitro media at the concentrations of 0, 1, 2, 3, and 4 mg.L-1. The highest PLBs formation, clump diameter, and fresh weight on D. mannii were obtained from explants cultured on medium supplemented with 1 mg.L-1 chitosan. After 14 weeks of culture, fresh weight, clump diameter and average number of PLBs of D.mannii were 29.52 %, 33.00 % and 35.88% greater at chitosan (1 mg.L-1) compared to control media.  D. mirbelianum treated with low chitosan concentrations (1 and 2 mg.L-1) had 65.71% more leaves than those without chitosan treatment. Chitosan, however, had an adverse effects on growth of both species at  4 mg.L-1. Medium contain 4 mg.L-1 chitosan induced PLBs proliferation of D.mannii, but inhibited PLBs proliferation of D.mirbelianum.  These results suggested that chitosan had important roles in PLB proliferation and could be used for the propagation of D. mannii PLBs in vitro. Keywords: rare orchid species, in-vitro
Evaluation of Commercial Sunflower (Helianthus annuus L.) Cultivars in Bogor, Indonesia for Ornamental and Nursery Production Aisyah, Syarifah Iis; Khotimah, Khotimah; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 1 No 1 (2014): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

A wide range of imported ornamental sunflower seeds have been marketed in Indonesia. A field evaluation was conducted on seven sunflower cultivars to investigate their germination, growth and development in humid tropical environment in Bogor, West Java, Indonesia and to determine their potential uses. Most of the tested cultivars, except for ‘Sungold Double’ and ‘Velvet Queen’, demonstrated good germination and growth. ‘Eclipse’ was highly susceptible to stem rot, which resulted in only 30% of the plants survived. ‘Little Leo’, ‘Teddy Bear’ and ‘Sungold Double’ were naturally compact, dwarf, and produced attractive blooms so they might be suitable as pot plants. ‘Hallo’, ‘Velvet Queen’, ‘Sunburst’, and ‘Eclipse’s plant height were more than 100 cm, had long stem and large blooms. These cultivars might be suitable as landscape plants.Keywords: morphological characteristics, West Java, pot plants
Photoautotrophic System: A Review and Potential Application for Plant Propagation In Vitro Krisantini, Krisantini; Wiendi, Ni Made Armini
Journal of Tropical Crop Science Vol 5 No 2 (2018): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

AbstractThe standard method of in vitro plant micro propagation uses of tightly closed culture bottles using agar media containing macro and micro nutrients and sucrose as a source of carbon for the explants. The closed bottle culture is usually kept in a temperature and light controlled environment which is lower and of different quality from the natural sunlight, resulting in high relative humidity and no air exchange inside the bottles.  Explants produced in vitro have malfunctioned stomata, undeveloped cuticles and lower leaf chlorophyll levels, and hyper hydration of the plantlets. Photoautotrophic tissue culture is micro propagation without or with a reduced sugar level in the culture media, so the growth or accumulation of carbohydrates of the explants is dependent fully upon photosynthesis and inorganic nutrient uptake. This method is usually combined with ventilation or CO2 enrichment, and recently, with incorporating porous materials such as vermiculite, gum or paper pulp to the agar media to promote better root system of the explants. This article discuss the advantages and disadvantages of the photoautotrophic micro propagation compared to the standard micro propagation methods, and provided the results of the photo autotrophic micro propagation studies conducted at Laboratory of Tissue Culture II of the Department of Agronomy and Horticulture, Bogor Agricultural University, Indonesia.
In Vitro Mutagenesis of Etlingera elatior by Gamma Ray Intermittent Irradiation Azzahra, Elmi Irmayanti; Aisyah, Syarifah Iis; Dinarti, Diny; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 5 No 3 (2018): Journal of Tropical Crop Science
Publisher : Journal of Tropical Crop Science

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Abstract

Etlingera elatior is tropical ornamental plant commonly called “torch ginger” from Zingiberaceae family. Conventional breeding of E. elatior is limited by cross incompatibility, poor fruit set and low seed production.  In this study irradiation of E. elatior with Gamma ray performed to induce mutation. This study was aimed to increase morphological diversity and to obtain unique morphological characters to increase the aesthetic value of E. elatior as ornamental plants and cut flower. Two genotypes of E. elatior, red and white flowers, were tested. The LD20, LD35, and LD50 were determined following intermittent (split dose) Gamma irradiation with a two-hour gap between each gamma ray shot. Red genotype E. elatior explants were irradiated with dose of 3 + 3 Gy (LD20); 4 + 4 Gy (LD35) and 5 + 5 Gy (LD50); white genotype were irradiated with a dose of 2 + 2 Gy (LD20); 2.8 + 2.8 Gy (LD35) and 3.7 + 3.7 Gy (LD50). Non-irradiated explants were set as control.  The results of this study indicated that the increase in dose of gamma ray irradiation changed shoot length and number of leaves in the red genotype per explant as compared to control. Morphological changes occurred in leaf shape at 5 + 5 Gy and 3.7 + 3.7 Gy and formation of variegated leaves at 2.8 + 2.8 Gy and 5 + 5 Gy. Keywords: mutation breeding, ornamental plant, split irradiation dose, torch ginger, Zingiberaceae